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The map label for this gene is cysH

Identifier: 152974946

GI number: 152974946

Start: 1254353

End: 1255057

Strand: Direct

Name: cysH

Synonym: Bcer98_1143

Alternate gene names: 152974946

Gene position: 1254353-1255057 (Clockwise)

Preceding gene: 152974945

Following gene: 152974947

Centisome position: 30.69

GC content: 37.59

Gene sequence:

>705_bases
ATGTTGACATATGAAACGTGGGAAGAAGACAGTATGTCTTTTTCTGAGAGAGATGAAACGAAAGGGGCGTTAGCTGTATT
AGAGTGGGCGTATAAACAATATAATGATGAAATTGTATATGCGTGCAGCTTTGGGGTAGAAGGGATGGTTCTTTTACATC
TTATCAATGAAGTAAATCCATTTGCTCAAGTTGTCTTTTTAGATACAAATGTTCATTTTTGCGAAACGTATGCGCTAATT
GAGCGAGTGCGTCAACGATTTCCAAAGTTGAATATTATTGAGAAACAACCCGGACTGACGCTCGAAGAACAAGAGGATAA
GTATGGAAAAGATTTATGGGAACATAATCCTAATCTTTGTTGTAAGCTTCGGAAAATTTTACCTCTTGAAGAATTGTTAG
CGAATAAAAATGCATGGATTTCAGGATTAAGAAGGGAACAATCTGAGACGAGGAAGCATACAAAATTTATAAATCAAGAT
CATCGCTTTCAATCGATTAAAATTTGTCCGCTTATTCATTGGACATGGAAAGAAGTGTGGCGTTATGTGTATAAACATAA
TTTGCCATACAACCCATTACATGACGTTGGTTATCCAAGTATAGGTTGTGAGAAATGTACATTACCTGTAGGCAAATATG
GAAGTTCAAGGGATGGCCGGTGGGCTGGATCGGTGAAAACGGAATGTGGACTTCATGACCAATGA

Upstream 100 bases:

>100_bases
GTACGCTTGAAATAAAAGCGCTGACTAGAAAAACTAGAGGCGGTTTTAATCTATTTTATTGGATTAAAACCGTCTTTTTG
CTTTTTATAGGGGGAAAAAC

Downstream 100 bases:

>100_bases
AGTTTTTAGTGATCTGAAATAGAGAAAAGAAGGAGTATAGAAACATGACTGCAACAAATTTTTTAGTAAATCGTATTGAT
GAAACATATGATACAGCAAA

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MLTYETWEEDSMSFSERDETKGALAVLEWAYKQYNDEIVYACSFGVEGMVLLHLINEVNPFAQVVFLDTNVHFCETYALI
ERVRQRFPKLNIIEKQPGLTLEEQEDKYGKDLWEHNPNLCCKLRKILPLEELLANKNAWISGLRREQSETRKHTKFINQD
HRFQSIKICPLIHWTWKEVWRYVYKHNLPYNPLHDVGYPSIGCEKCTLPVGKYGSSRDGRWAGSVKTECGLHDQ

Sequences:

>Translated_234_residues
MLTYETWEEDSMSFSERDETKGALAVLEWAYKQYNDEIVYACSFGVEGMVLLHLINEVNPFAQVVFLDTNVHFCETYALI
ERVRQRFPKLNIIEKQPGLTLEEQEDKYGKDLWEHNPNLCCKLRKILPLEELLANKNAWISGLRREQSETRKHTKFINQD
HRFQSIKICPLIHWTWKEVWRYVYKHNLPYNPLHDVGYPSIGCEKCTLPVGKYGSSRDGRWAGSVKTECGLHDQ
>Mature_234_residues
MLTYETWEEDSMSFSERDETKGALAVLEWAYKQYNDEIVYACSFGVEGMVLLHLINEVNPFAQVVFLDTNVHFCETYALI
ERVRQRFPKLNIIEKQPGLTLEEQEDKYGKDLWEHNPNLCCKLRKILPLEELLANKNAWISGLRREQSETRKHTKFINQD
HRFQSIKICPLIHWTWKEVWRYVYKHNLPYNPLHDVGYPSIGCEKCTLPVGKYGSSRDGRWAGSVKTECGLHDQ

Specific function: Reduction of activated sulfate into sulfite

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily

Homologues:

Organism=Escherichia coli, GI1789121, Length=223, Percent_Identity=33.6322869955157, Blast_Score=110, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6325425, Length=203, Percent_Identity=32.512315270936, Blast_Score=108, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSH_BACCN (A7GMW0)

Other databases:

- EMBL:   CP000764
- RefSeq:   YP_001374463.1
- ProteinModelPortal:   A7GMW0
- SMR:   A7GMW0
- STRING:   A7GMW0
- EnsemblBacteria:   EBBACT00000037449
- GeneID:   5344600
- GenomeReviews:   CP000764_GR
- KEGG:   bcy:Bcer98_1143
- eggNOG:   COG0175
- GeneTree:   EBGT00050000002870
- HOGENOM:   HBG758022
- OMA:   KVEPAHR
- ProtClustDB:   CLSK916236
- BioCyc:   BCER315749:BCER98_1143-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00063
- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00434

Pfam domain/function: PF01507 PAPS_reduct

EC number: =1.8.4.8

Molecular weight: Translated: 27514; Mature: 27514

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTYETWEEDSMSFSERDETKGALAVLEWAYKQYNDEIVYACSFGVEGMVLLHLINEVNP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCC
FAQVVFLDTNVHFCETYALIERVRQRFPKLNIIEKQPGLTLEEQEDKYGKDLWEHNPNLC
CCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCHH
CKLRKILPLEELLANKNAWISGLRREQSETRKHTKFINQDHRFQSIKICPLIHWTWKEVW
HHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEHHHHHHHH
RYVYKHNLPYNPLHDVGYPSIGCEKCTLPVGKYGSSRDGRWAGSVKTECGLHDQ
HHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLTYETWEEDSMSFSERDETKGALAVLEWAYKQYNDEIVYACSFGVEGMVLLHLINEVNP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCC
FAQVVFLDTNVHFCETYALIERVRQRFPKLNIIEKQPGLTLEEQEDKYGKDLWEHNPNLC
CCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCHH
CKLRKILPLEELLANKNAWISGLRREQSETRKHTKFINQDHRFQSIKICPLIHWTWKEVW
HHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEHHHHHHHH
RYVYKHNLPYNPLHDVGYPSIGCEKCTLPVGKYGSSRDGRWAGSVKTECGLHDQ
HHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA