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The map label for this gene is queE [H]

Identifier: 152974870

GI number: 152974870

Start: 1181937

End: 1182653

Strand: Direct

Name: queE [H]

Synonym: Bcer98_1066

Alternate gene names: 152974870

Gene position: 1181937-1182653 (Clockwise)

Preceding gene: 152974869

Following gene: 152974871

Centisome position: 28.92

GC content: 35.7

Gene sequence:

>717_bases
ATGAGTAAAATCCCCGTTTTAGAAATATTCGGTCCAACCATTCAAGGAGAAGGAATGGTTGTGGGGCAAAAAACGATGTT
TATTCGTACAGCGGGATGTGATTATAGTTGCGCATGGTGTGATTCTGCTTTTACTTGGGATGGATCGGCGAAAGATCAGA
TTCGACAAATGACCCCAGAAGAAATTTGGAAGGAACTTATTGAGATTGGTGGAGAAAACTTTTCGCATGTAACAATTTCA
GGTGGAAATCCAGTGTTACTGAAACATATGAAAGAACTTTTAGAGAGATTAAAAGAGAATAACATTCGGACCGCCATTGA
AACGCAAGGGAGTAAATGGCAAGAATGGCTTTTGGACATAGATGAAATTACAATTTCACCGAAACCACCAAGTTCAAAAA
TGAAAACAAATTTTACGATGTTAGACTTAGTAGTAGAAAGATTAGTAGATAAAAATATGAGTTTAAAAGTAGTTATATTT
GATGATGATGACTTTGAATATGCAAAGAACGTTCATGAACGTTATCCTCATGTACCTTTTTTCCTACAGGTTGGAAACGA
TGATACAAAAACAGCAGAGGACGCAGTGTTAATTCCTAAATTATTACAGAAATATGAATGGTTAATTGAAAAGGCAGTTC
ATAGTAAAGAAATGAATGATGTAAAAGTATTACCTCAGCTTCATGCCTTAGTATGGGGAAATAAGCGAGGCGTATAA

Upstream 100 bases:

>100_bases
GGAGGCCGAGCAGAAGAGTATAAAGGAGCTCGAGTTGAATTTGTTCGTCTGTTTGAAACGCCGACAAGTTATACAGAAGT
AAGACGGGAGTGGATGCTCG

Downstream 100 bases:

>100_bases
GTGAATAGAAGGGATGTTTAAAGATGGCAGGAAGATTAGATGAAGATTTAAAAGATGTAACGTTGCTTGGAAATCAAAAT
ACAAAATATTTATTTGAATA

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: CDG synthase; Queuosine biosynthesis protein queE [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MSKIPVLEIFGPTIQGEGMVVGQKTMFIRTAGCDYSCAWCDSAFTWDGSAKDQIRQMTPEEIWKELIEIGGENFSHVTIS
GGNPVLLKHMKELLERLKENNIRTAIETQGSKWQEWLLDIDEITISPKPPSSKMKTNFTMLDLVVERLVDKNMSLKVVIF
DDDDFEYAKNVHERYPHVPFFLQVGNDDTKTAEDAVLIPKLLQKYEWLIEKAVHSKEMNDVKVLPQLHALVWGNKRGV

Sequences:

>Translated_238_residues
MSKIPVLEIFGPTIQGEGMVVGQKTMFIRTAGCDYSCAWCDSAFTWDGSAKDQIRQMTPEEIWKELIEIGGENFSHVTIS
GGNPVLLKHMKELLERLKENNIRTAIETQGSKWQEWLLDIDEITISPKPPSSKMKTNFTMLDLVVERLVDKNMSLKVVIF
DDDDFEYAKNVHERYPHVPFFLQVGNDDTKTAEDAVLIPKLLQKYEWLIEKAVHSKEMNDVKVLPQLHALVWGNKRGV
>Mature_237_residues
SKIPVLEIFGPTIQGEGMVVGQKTMFIRTAGCDYSCAWCDSAFTWDGSAKDQIRQMTPEEIWKELIEIGGENFSHVTISG
GNPVLLKHMKELLERLKENNIRTAIETQGSKWQEWLLDIDEITISPKPPSSKMKTNFTMLDLVVERLVDKNMSLKVVIFD
DDDFEYAKNVHERYPHVPFFLQVGNDDTKTAEDAVLIPKLLQKYEWLIEKAVHSKEMNDVKVLPQLHALVWGNKRGV

Specific function: Catalyzes the conversion of 6-carboxy-5,6,7,8- tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG) [H]

COG id: COG0602

COG function: function code O; Organic radical activating enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. 7-carboxy-7- deazaguanine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1789139, Length=159, Percent_Identity=29.559748427673, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017742
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 27236; Mature: 27104

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIPVLEIFGPTIQGEGMVVGQKTMFIRTAGCDYSCAWCDSAFTWDGSAKDQIRQMTPE
CCCCCEEEEECCCCCCCCEEEECEEEEEEECCCCCEEEECCCCCCCCCCCHHHHHCCCHH
EIWKELIEIGGENFSHVTISGGNPVLLKHMKELLERLKENNIRTAIETQGSKWQEWLLDI
HHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHH
DEITISPKPPSSKMKTNFTMLDLVVERLVDKNMSLKVVIFDDDDFEYAKNVHERYPHVPF
HHEEECCCCCCCHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCCCE
FLQVGNDDTKTAEDAVLIPKLLQKYEWLIEKAVHSKEMNDVKVLPQLHALVWGNKRGV
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SKIPVLEIFGPTIQGEGMVVGQKTMFIRTAGCDYSCAWCDSAFTWDGSAKDQIRQMTPE
CCCCEEEEECCCCCCCCEEEECEEEEEEECCCCCEEEECCCCCCCCCCCHHHHHCCCHH
EIWKELIEIGGENFSHVTISGGNPVLLKHMKELLERLKENNIRTAIETQGSKWQEWLLDI
HHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHH
DEITISPKPPSSKMKTNFTMLDLVVERLVDKNMSLKVVIFDDDDFEYAKNVHERYPHVPF
HHEEECCCCCCCHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCCCE
FLQVGNDDTKTAEDAVLIPKLLQKYEWLIEKAVHSKEMNDVKVLPQLHALVWGNKRGV
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]