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The map label for this gene is topX [H]

Identifier: 152974785

GI number: 152974785

Start: 1097955

End: 1098245

Strand: Direct

Name: topX [H]

Synonym: Bcer98_0977

Alternate gene names: 152974785

Gene position: 1097955-1098245 (Clockwise)

Preceding gene: 152974784

Following gene: 152974786

Centisome position: 26.86

GC content: 34.71

Gene sequence:

>291_bases
TTGTTTAAGGAGGAAAAAGTTGTGGGGAAAACGTTATTTATTGCGGAAAAACCGAAAGTTGCCAATGAAATTATGAAATT
GCCGCGATTCCATCACTCACAAAAGTACATTAGTAGCAAACCTTATTATGAAAATAATCATTATATTGTCAGTTGGTGCC
GTGGGCATCTGCTGGAACTAAAAAATCCGGAAGAGATGGATCCAATGTATAAAGTGTTTAAGTTAGAGCATTTACCCTTA
ATTTATCAGCCAGATTATAAAGTGAAACAAGAAAAAGCTGAACAATTGTAG

Upstream 100 bases:

>100_bases
AAATAGAGGGAATGTTATCATATAAAGTAATCCAATACTAAAAAATCATATTGCAAAAGGACATACCTACGCATAACCAT
AGCGTGGGTGTGTCCTTTTT

Downstream 100 bases:

>100_bases
ATTGTCAACCAAATTTGACCCACCTACTACCAATCGTTGTCGCATCAGTCCAGCTCAAGGGTATCGTGAATCTATTATTC
GTCTTGAAAAAGAGGGAAAA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 96; Mature: 96

Protein sequence:

>96_residues
MFKEEKVVGKTLFIAEKPKVANEIMKLPRFHHSQKYISSKPYYENNHYIVSWCRGHLLELKNPEEMDPMYKVFKLEHLPL
IYQPDYKVKQEKAEQL

Sequences:

>Translated_96_residues
MFKEEKVVGKTLFIAEKPKVANEIMKLPRFHHSQKYISSKPYYENNHYIVSWCRGHLLELKNPEEMDPMYKVFKLEHLPL
IYQPDYKVKQEKAEQL
>Mature_96_residues
MFKEEKVVGKTLFIAEKPKVANEIMKLPRFHHSQKYISSKPYYENNHYIVSWCRGHLLELKNPEEMDPMYKVFKLEHLPL
IYQPDYKVKQEKAEQL

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. May play an important role in either the maintenance of, or expression from, the virulence plasmid. Its influence on expression could be specific

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 11611; Mature: 11611

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKEEKVVGKTLFIAEKPKVANEIMKLPRFHHSQKYISSKPYYENNHYIVSWCRGHLLEL
CCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCHHHHCCCCCCCCCCEEEEECCCCEECC
KNPEEMDPMYKVFKLEHLPLIYQPDYKVKQEKAEQL
CCCCCCCHHHHHHHHCCCCEEECCCCCCHHHHHCCC
>Mature Secondary Structure
MFKEEKVVGKTLFIAEKPKVANEIMKLPRFHHSQKYISSKPYYENNHYIVSWCRGHLLEL
CCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCHHHHCCCCCCCCCCEEEEECCCCEECC
KNPEEMDPMYKVFKLEHLPLIYQPDYKVKQEKAEQL
CCCCCCCHHHHHHHHCCCCEEECCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8152371; 10515943; 12004073 [H]