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The map label for this gene is spsI [H]

Identifier: 152974747

GI number: 152974747

Start: 1052485

End: 1053258

Strand: Direct

Name: spsI [H]

Synonym: Bcer98_0937

Alternate gene names: 152974747

Gene position: 1052485-1053258 (Clockwise)

Preceding gene: 152974746

Following gene: 152974748

Centisome position: 25.75

GC content: 37.21

Gene sequence:

>774_bases
TTGATTACTAGTATAAGGAGTAAAGGAGAGATTTGGGTGAAAGGGATTATTTTAGCTGGAGGAACGGGATCGCGGCTATA
CCCCATTACAAAAGTAACGAATAAGCATTTGCTTCCAGTTGGTCGTTATCCGATGATTTATCATGCTGTTTATAAGATGA
AACAATGCGGTATTACCGATATTATGATTGTTACGGGAAAAGAACATATGGGGGATGTTGTTAATTTTTTAGGAAGCGGT
CATGATTTTCATGTTTCATTCACGTATCGCGTGCAGGATAGAGCCGGAGGGATAGCTGAGGCGCTCGGACTTTGTGAACA
ATTTGTTGGAAAAGATCGGATGCTTGTTATACTAGGAGATAATATTTTTTCAGATAATATTTATCCATACGTTCAGGAAT
ATGTGAAGCAAGAAAAAGGAGCAAAGGTATTATTGCAGGAAGTGGAAGATCCAGAGAGATTTGGAGTCCCGGTTATACAA
AATGATAAAATTATTGAAATTGAAGAAAAGCCTAAAAAACCGAAAAGTTCATATGCAGTGACAGGAATTTATATGTATGA
CGCTGAGGTGTTTTCGTATATTAAGCAATTAACACCTTCAGAAAGAGGAGAGCTTGAGATTACGGATTTAAATAATCGGT
ATTTGAAACGTGGCTTATTATCTTATAATATTATGTCTGGTTGGTGGACAGACGCAGGAACACATGCATCATTGCAGAGA
GCAAATGCATTAGCGTGTCATTTAGATTTTGGTCAACAATTTAATGGGGAATAG

Upstream 100 bases:

>100_bases
TATGAGAAATATAGACGAAAATTTGTAGAACACTATATGAAACCACTATTTTCATTATAAAGTAGTGGTTTCATAGTTAA
GTATAGAGATAAGGAATAGA

Downstream 100 bases:

>100_bases
GTGGGTATATGGAAATAGTTGAAACACATTTTCAGCATGCTTTATTATTACAACCTCGTTTATTTGAAGATCATCGTGGT
TTTTTTACAGAAAGTTATAA

Product: nucleotidyl transferase

Products: diphosphate; dTDPglucose

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MITSIRSKGEIWVKGIILAGGTGSRLYPITKVTNKHLLPVGRYPMIYHAVYKMKQCGITDIMIVTGKEHMGDVVNFLGSG
HDFHVSFTYRVQDRAGGIAEALGLCEQFVGKDRMLVILGDNIFSDNIYPYVQEYVKQEKGAKVLLQEVEDPERFGVPVIQ
NDKIIEIEEKPKKPKSSYAVTGIYMYDAEVFSYIKQLTPSERGELEITDLNNRYLKRGLLSYNIMSGWWTDAGTHASLQR
ANALACHLDFGQQFNGE

Sequences:

>Translated_257_residues
MITSIRSKGEIWVKGIILAGGTGSRLYPITKVTNKHLLPVGRYPMIYHAVYKMKQCGITDIMIVTGKEHMGDVVNFLGSG
HDFHVSFTYRVQDRAGGIAEALGLCEQFVGKDRMLVILGDNIFSDNIYPYVQEYVKQEKGAKVLLQEVEDPERFGVPVIQ
NDKIIEIEEKPKKPKSSYAVTGIYMYDAEVFSYIKQLTPSERGELEITDLNNRYLKRGLLSYNIMSGWWTDAGTHASLQR
ANALACHLDFGQQFNGE
>Mature_257_residues
MITSIRSKGEIWVKGIILAGGTGSRLYPITKVTNKHLLPVGRYPMIYHAVYKMKQCGITDIMIVTGKEHMGDVVNFLGSG
HDFHVSFTYRVQDRAGGIAEALGLCEQFVGKDRMLVILGDNIFSDNIYPYVQEYVKQEKGAKVLLQEVEDPERFGVPVIQ
NDKIIEIEEKPKKPKSSYAVTGIYMYDAEVFSYIKQLTPSERGELEITDLNNRYLKRGLLSYNIMSGWWTDAGTHASLQR
ANALACHLDFGQQFNGE

Specific function: Catalyzes The Formation Of Dtdp-Glucose, From Dttp And Glucose 1-Phosphate, As Well As Its Pyrophosphorolysis. [C]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788351, Length=240, Percent_Identity=44.1666666666667, Blast_Score=194, Evalue=5e-51,
Organism=Escherichia coli, GI1790224, Length=247, Percent_Identity=42.1052631578947, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI1787488, Length=261, Percent_Identity=23.7547892720307, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133931050, Length=251, Percent_Identity=22.3107569721116, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320148, Length=226, Percent_Identity=26.5486725663717, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: 2.7.7.24

Molecular weight: Translated: 28978; Mature: 28978

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITSIRSKGEIWVKGIILAGGTGSRLYPITKVTNKHLLPVGRYPMIYHAVYKMKQCGITD
CCCCCCCCCCEEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEE
IMIVTGKEHMGDVVNFLGSGHDFHVSFTYRVQDRAGGIAEALGLCEQFVGKDRMLVILGD
EEEEECCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECC
NIFSDNIYPYVQEYVKQEKGAKVLLQEVEDPERFGVPVIQNDKIIEIEEKPKKPKSSYAV
CCCCCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHCCCEECCCCEEEECCCCCCCCCCEEE
TGIYMYDAEVFSYIKQLTPSERGELEITDLNNRYLKRGLLSYNIMSGWWTDAGTHASLQR
EEEEEECHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHEEEECCCCCCCCCCCHHHHH
ANALACHLDFGQQFNGE
CCEEEEEEECCCCCCCC
>Mature Secondary Structure
MITSIRSKGEIWVKGIILAGGTGSRLYPITKVTNKHLLPVGRYPMIYHAVYKMKQCGITD
CCCCCCCCCCEEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEE
IMIVTGKEHMGDVVNFLGSGHDFHVSFTYRVQDRAGGIAEALGLCEQFVGKDRMLVILGD
EEEEECCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECC
NIFSDNIYPYVQEYVKQEKGAKVLLQEVEDPERFGVPVIQNDKIIEIEEKPKKPKSSYAV
CCCCCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHCCCEECCCCEEEECCCCCCCCCCEEE
TGIYMYDAEVFSYIKQLTPSERGELEITDLNNRYLKRGLLSYNIMSGWWTDAGTHASLQR
EEEEEECHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHEEEECCCCCCCCCCCHHHHH
ANALACHLDFGQQFNGE
CCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTTP; alpha-D-glucose 1-phosphate

Specific reaction: dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-glucose

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]