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The map label for this gene is ybbF [H]

Identifier: 152974496

GI number: 152974496

Start: 757059

End: 758426

Strand: Direct

Name: ybbF [H]

Synonym: Bcer98_0671

Alternate gene names: 152974496

Gene position: 757059-758426 (Clockwise)

Preceding gene: 152974495

Following gene: 152974497

Centisome position: 18.52

GC content: 41.3

Gene sequence:

>1368_bases
ATGAAGAAAGAACAGCGGATGGCAAATGACATTACAGAGCAACTTGGGGGAATACAAAATGTACGCAGCATTGCACATTG
TATGACGAGATTACGTCTTACACTTCATGACGAAAGTAAAGTGAATATGGATCAATTGAAAAAAGTTGACGGTGTAATGG
GCGTTATTGAAGATGAAACATTACAAGTTGTAGTTGGACCGGGAACTGTCAATAAAGTAGCGGCTGAAATGGAAGCGTTG
ACAGGGCTGCGAATGGGGGAAGTGGCTCATGATAACCATCTAGAAGACATTGGACAAGAAATGAAATCCGCCATTAAGAA
GAAAAATAATACACCAGTGAAAAACTTTTTGCGAAAAGTAGGAAGTATCTTTATTCCGTTAATTCCTGGTCTTGTTGCTT
CAGGAATTATAAATGGGGTTGCGAATTTTGCTAAAAATGCAGGGGCAGATCCGAATGCGACGTGGCTGCAAATCTTGCTT
TTAATGGGTGGCGGTATTTTTACATTTTTAGGGATTTTAGTGGGGTGGAATACAGCGAAAGAATTTGGTGGTACACCAGT
TCTCGGCGCAATCGCGGGTATTTTAATTTTCAACCCGGCTATGGCGGATGTGAAAGTATTTGGTGAAGCGTTAGTACCGG
GGCGCGGTGGTTTATTTGCAGTAATCTTTGCAGCATGGCTCATGGTTATTGTGGAAAGACAAGTTCGAAAAGCGGTTCCA
AATGCTGTTGATATTATAGTGACGCCGCTTATTACAGTATTAGTTGTCAGTTTAGTAACGATGCTAGCTATTCAGCCAGT
TGCGGGATTATTATCCGATGGAATTACAAGTGGAATAAATGCAATTTTAAATATTGGAGGAGTTATTGCAGGTGCGGTGC
TTGCAGGAACATTCTTGCCCCTCGTTATGGTTGGACTACATCACGGACTTACACCGATTCACATGGAATTTATTAACCAA
ACACATGTAACGCCACTGCTACCAGTTTTGGCAATGGCAGGAGCTGGGCAAGTAGGGGCAGCGATTGCGATTTTCGTGAA
AACAAAAAATAAACGCCTTCGTAATGTCATTAAAGGGGGACTGCCAGTTGGATTTTTAGGAATTGGCGAACCGTTATTAT
ACGGGGTGACATTACCGCTTGGGAAACCATTTATTACAGCTTGTTTAGGTGCGGCTGTTGGCGGTGCTTTTCAAGCAGTT
ATGAAAACGGCTTCACTTGGAATCGGCGTATCAGGGATATCGTTAATTCCGCTCATTGCCGATAATAAATATTTACTCTA
TTTCCTTGGCTTAGTAATCGCTTATATTTTCGGCTTTATTTTTACGTATTTCTTCGGATTTAAAGAAGAAATGGCAGAAA
ATATATAG

Upstream 100 bases:

>100_bases
TATTACTACAGTGTGAATATGGGGAAGCACTTGAAAAATTAAAAGATGCAAAAGGATTTGTGAAGAAGGCGCTATAGAAA
TGGAGAGGAGAGATTCTACC

Downstream 100 bases:

>100_bases
GGGCTATCCTCTTTTTTTCCAGGGGAACTTGAACTGTACCCTATAAAATGGACAGTTTCATAAAAAGACCTTTAAAAATC
AAGCACTTAAAAGATGGTTC

Product: PTS system, sucrose-specific IIBC component

Products: NA

Alternate protein names: Phosphotransferase enzyme IIB component; PTS system EIIB component; Permease IIC component; PTS system EIIC component [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MKKEQRMANDITEQLGGIQNVRSIAHCMTRLRLTLHDESKVNMDQLKKVDGVMGVIEDETLQVVVGPGTVNKVAAEMEAL
TGLRMGEVAHDNHLEDIGQEMKSAIKKKNNTPVKNFLRKVGSIFIPLIPGLVASGIINGVANFAKNAGADPNATWLQILL
LMGGGIFTFLGILVGWNTAKEFGGTPVLGAIAGILIFNPAMADVKVFGEALVPGRGGLFAVIFAAWLMVIVERQVRKAVP
NAVDIIVTPLITVLVVSLVTMLAIQPVAGLLSDGITSGINAILNIGGVIAGAVLAGTFLPLVMVGLHHGLTPIHMEFINQ
THVTPLLPVLAMAGAGQVGAAIAIFVKTKNKRLRNVIKGGLPVGFLGIGEPLLYGVTLPLGKPFITACLGAAVGGAFQAV
MKTASLGIGVSGISLIPLIADNKYLLYFLGLVIAYIFGFIFTYFFGFKEEMAENI

Sequences:

>Translated_455_residues
MKKEQRMANDITEQLGGIQNVRSIAHCMTRLRLTLHDESKVNMDQLKKVDGVMGVIEDETLQVVVGPGTVNKVAAEMEAL
TGLRMGEVAHDNHLEDIGQEMKSAIKKKNNTPVKNFLRKVGSIFIPLIPGLVASGIINGVANFAKNAGADPNATWLQILL
LMGGGIFTFLGILVGWNTAKEFGGTPVLGAIAGILIFNPAMADVKVFGEALVPGRGGLFAVIFAAWLMVIVERQVRKAVP
NAVDIIVTPLITVLVVSLVTMLAIQPVAGLLSDGITSGINAILNIGGVIAGAVLAGTFLPLVMVGLHHGLTPIHMEFINQ
THVTPLLPVLAMAGAGQVGAAIAIFVKTKNKRLRNVIKGGLPVGFLGIGEPLLYGVTLPLGKPFITACLGAAVGGAFQAV
MKTASLGIGVSGISLIPLIADNKYLLYFLGLVIAYIFGFIFTYFFGFKEEMAENI
>Mature_455_residues
MKKEQRMANDITEQLGGIQNVRSIAHCMTRLRLTLHDESKVNMDQLKKVDGVMGVIEDETLQVVVGPGTVNKVAAEMEAL
TGLRMGEVAHDNHLEDIGQEMKSAIKKKNNTPVKNFLRKVGSIFIPLIPGLVASGIINGVANFAKNAGADPNATWLQILL
LMGGGIFTFLGILVGWNTAKEFGGTPVLGAIAGILIFNPAMADVKVFGEALVPGRGGLFAVIFAAWLMVIVERQVRKAVP
NAVDIIVTPLITVLVVSLVTMLAIQPVAGLLSDGITSGINAILNIGGVIAGAVLAGTFLPLVMVGLHHGLTPIHMEFINQ
THVTPLLPVLAMAGAGQVGAAIAIFVKTKNKRLRNVIKGGLPVGFLGIGEPLLYGVTLPLGKPFITACLGAAVGGAFQAV
MKTASLGIGVSGISLIPLIADNKYLLYFLGLVIAYIFGFIFTYFFGFKEEMAENI

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788769, Length=481, Percent_Identity=38.2536382536383, Blast_Score=262, Evalue=4e-71,
Organism=Escherichia coli, GI1790159, Length=425, Percent_Identity=29.4117647058824, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI2367362, Length=437, Percent_Identity=23.7986270022883, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI48994906, Length=477, Percent_Identity=25.7861635220126, Blast_Score=125, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 48045; Mature: 48045

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEQRMANDITEQLGGIQNVRSIAHCMTRLRLTLHDESKVNMDQLKKVDGVMGVIEDET
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCC
LQVVVGPGTVNKVAAEMEALTGLRMGEVAHDNHLEDIGQEMKSAIKKKNNTPVKNFLRKV
EEEEECCCHHHHHHHHHHHHHCCHHCCHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHH
GSIFIPLIPGLVASGIINGVANFAKNAGADPNATWLQILLLMGGGIFTFLGILVGWNTAK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCHHH
EFGGTPVLGAIAGILIFNPAMADVKVFGEALVPGRGGLFAVIFAAWLMVIVERQVRKAVP
HCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NAVDIIVTPLITVLVVSLVTMLAIQPVAGLLSDGITSGINAILNIGGVIAGAVLAGTFLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVMVGLHHGLTPIHMEFINQTHVTPLLPVLAMAGAGQVGAAIAIFVKTKNKRLRNVIKGG
HHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHCC
LPVGFLGIGEPLLYGVTLPLGKPFITACLGAAVGGAFQAVMKTASLGIGVSGISLIPLIA
CCEEECCCCCHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
DNKYLLYFLGLVIAYIFGFIFTYFFGFKEEMAENI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKEQRMANDITEQLGGIQNVRSIAHCMTRLRLTLHDESKVNMDQLKKVDGVMGVIEDET
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCC
LQVVVGPGTVNKVAAEMEALTGLRMGEVAHDNHLEDIGQEMKSAIKKKNNTPVKNFLRKV
EEEEECCCHHHHHHHHHHHHHCCHHCCHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHH
GSIFIPLIPGLVASGIINGVANFAKNAGADPNATWLQILLLMGGGIFTFLGILVGWNTAK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCHHH
EFGGTPVLGAIAGILIFNPAMADVKVFGEALVPGRGGLFAVIFAAWLMVIVERQVRKAVP
HCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NAVDIIVTPLITVLVVSLVTMLAIQPVAGLLSDGITSGINAILNIGGVIAGAVLAGTFLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVMVGLHHGLTPIHMEFINQTHVTPLLPVLAMAGAGQVGAAIAIFVKTKNKRLRNVIKGG
HHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHCC
LPVGFLGIGEPLLYGVTLPLGKPFITACLGAAVGGAFQAVMKTASLGIGVSGISLIPLIA
CCEEECCCCCHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
DNKYLLYFLGLVIAYIFGFIFTYFFGFKEEMAENI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]