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The map label for this gene is thiO [H]

Identifier: 152974442

GI number: 152974442

Start: 707778

End: 708887

Strand: Direct

Name: thiO [H]

Synonym: Bcer98_0617

Alternate gene names: 152974442

Gene position: 707778-708887 (Clockwise)

Preceding gene: 152974441

Following gene: 152974443

Centisome position: 17.32

GC content: 39.37

Gene sequence:

>1110_bases
ATGAGTAAGAAGTATGATGTAGCTATTATTGGCGGTGGTGTAATTGGTAGTTCAGTTGCACATTTTCTAGCAGAAAGAAG
TTGCCATGTTGCAATTGTTGAGAAGCAAAGGGTTGCTTCGGAAGCATCAAAAGCAGCGGCAGGACTTCTTGGAGTACAGG
CGGAATGGGATGTATATAACCCGCTCTTTGAACTTGCTAGAGAAAGCCGTGCAATATTTCCGCAACTTGCAAAGGCGTTA
CGAGAAAAAACGGGAATTGATATTGGATATGAAGAGAAAGGGATATATCGGATTGCTCAAAATGAAGAGGAGAAAACAAG
AATACGAAATATAATGAACTGGCAACAAGAAACAGGTGAGGAATCTTATTTTCTAACAGGAGATGAGCTTCGCCAAAAGG
AACCGTTTCTTTCTTCCTCCATTATCGGCGCTGTCTATTATCCCAAAGATGGTCATGTTATTGCGCCAGAACTAACGAAA
GCGTTCGCACACTCTGCGGCCATTTCTGGTACTGATATATATGAACAAACAGAAGTATTTGATATTCGAGTTGAAAAAAA
TAGAGTGTGCGGAATTGTTACGAGCGAAGGCTTTATTTCATGTGAAAAAGTAGTCATTGCAGGTGGTTCATGGAGTACAA
AACTACTTCAATATTTTCATAGAGATTGGGGTACTTATCCTGTAAAAGGGGAAGTAGTCGCAGTAAAAAGCTATAAGCCA
TTAGTAAGAGCTCCGATTTTCCAAGACAGATTTTACATCGCACCAAAACGTGGTGGTCGCTATGTAATTGGAGCAACAAT
GAAACCTCATACGTTTAACAAATCTGTACAACCAAAAAGTATCATTTCGATATTAGAACGTGCGTATTCAATTTTACCAG
CTTTAAAAGATGCGGAGTGGGAAACCGCATGGGCAGGGCTTCGTCCGCAATCCAATCATGACGTTCCTTATATGGGAGAG
CATGAGGAAATAAAAGGGTTATATGCTTGTACAGGTCATTACCGAAATGGTATTTTATTAAGTCCTATTTCTGGTCAATA
TATGGCCGATATCATAGAAGGAAAACAAACTAATCATTTGTTAGATTCATTGTTTTCAAAGTCAGTTTAG

Upstream 100 bases:

>100_bases
GTGAGTGGTATTGCAGTTATGTCTGGAATTGTAAGCGATCGTAATCCATACGAGAAAGCAAGATTGTATAAGGAAATAAT
AAGAAAGTGGGCGGAAAATC

Downstream 100 bases:

>100_bases
AAGGGGGATGGAAGCTTGTATTTGAAAATTAATGGTAGGCAAGTAGAGGTGCCAGAACATATTAAAACGGTAGCGGAATT
GCTTACTCACTTAGAGTTGG

Product: glycine oxidase ThiO

Products: NA

Alternate protein names: GO [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MSKKYDVAIIGGGVIGSSVAHFLAERSCHVAIVEKQRVASEASKAAAGLLGVQAEWDVYNPLFELARESRAIFPQLAKAL
REKTGIDIGYEEKGIYRIAQNEEEKTRIRNIMNWQQETGEESYFLTGDELRQKEPFLSSSIIGAVYYPKDGHVIAPELTK
AFAHSAAISGTDIYEQTEVFDIRVEKNRVCGIVTSEGFISCEKVVIAGGSWSTKLLQYFHRDWGTYPVKGEVVAVKSYKP
LVRAPIFQDRFYIAPKRGGRYVIGATMKPHTFNKSVQPKSIISILERAYSILPALKDAEWETAWAGLRPQSNHDVPYMGE
HEEIKGLYACTGHYRNGILLSPISGQYMADIIEGKQTNHLLDSLFSKSV

Sequences:

>Translated_369_residues
MSKKYDVAIIGGGVIGSSVAHFLAERSCHVAIVEKQRVASEASKAAAGLLGVQAEWDVYNPLFELARESRAIFPQLAKAL
REKTGIDIGYEEKGIYRIAQNEEEKTRIRNIMNWQQETGEESYFLTGDELRQKEPFLSSSIIGAVYYPKDGHVIAPELTK
AFAHSAAISGTDIYEQTEVFDIRVEKNRVCGIVTSEGFISCEKVVIAGGSWSTKLLQYFHRDWGTYPVKGEVVAVKSYKP
LVRAPIFQDRFYIAPKRGGRYVIGATMKPHTFNKSVQPKSIISILERAYSILPALKDAEWETAWAGLRPQSNHDVPYMGE
HEEIKGLYACTGHYRNGILLSPISGQYMADIIEGKQTNHLLDSLFSKSV
>Mature_368_residues
SKKYDVAIIGGGVIGSSVAHFLAERSCHVAIVEKQRVASEASKAAAGLLGVQAEWDVYNPLFELARESRAIFPQLAKALR
EKTGIDIGYEEKGIYRIAQNEEEKTRIRNIMNWQQETGEESYFLTGDELRQKEPFLSSSIIGAVYYPKDGHVIAPELTKA
FAHSAAISGTDIYEQTEVFDIRVEKNRVCGIVTSEGFISCEKVVIAGGSWSTKLLQYFHRDWGTYPVKGEVVAVKSYKPL
VRAPIFQDRFYIAPKRGGRYVIGATMKPHTFNKSVQPKSIISILERAYSILPALKDAEWETAWAGLRPQSNHDVPYMGEH
EEIKGLYACTGHYRNGILLSPISGQYMADIIEGKQTNHLLDSLFSKSV

Specific function: Catalyzes the FAD-dependent oxidative deamination of various amines and D-amino acids to yield the corresponding alpha- keto acids, ammonia/amine, and hydrogen peroxide. Oxidizes sarcosine (N-methylglycine), N-ethylglycine and glycine. Can also oxidize th

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DAO family [H]

Homologues:

Organism=Homo sapiens, GI197927446, Length=372, Percent_Identity=24.1935483870968, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI21361378, Length=372, Percent_Identity=24.1935483870968, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI24797151, Length=388, Percent_Identity=22.9381443298969, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI194306651, Length=211, Percent_Identity=27.0142180094787, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1787438, Length=410, Percent_Identity=24.6341463414634, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71994045, Length=390, Percent_Identity=23.3333333333333, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71994052, Length=397, Percent_Identity=22.9219143576826, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI32563613, Length=382, Percent_Identity=22.7748691099476, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI20130091, Length=369, Percent_Identity=24.9322493224932, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR012727 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.3.19 [H]

Molecular weight: Translated: 41201; Mature: 41070

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKYDVAIIGGGVIGSSVAHFLAERSCHVAIVEKQRVASEASKAAAGLLGVQAEWDVYN
CCCCEEEEEEECCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHEECCCCCCHHH
PLFELARESRAIFPQLAKALREKTGIDIGYEEKGIYRIAQNEEEKTRIRNIMNWQQETGE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCC
ESYFLTGDELRQKEPFLSSSIIGAVYYPKDGHVIAPELTKAFAHSAAISGTDIYEQTEVF
CCEEECCHHHHHCCCCHHCCCEEEEEECCCCCEECHHHHHHHHHHHCCCCCCCCCCCEEE
DIRVEKNRVCGIVTSEGFISCEKVVIAGGSWSTKLLQYFHRDWGTYPVKGEVVAVKSYKP
EEEEECCCEEEEEECCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCC
LVRAPIFQDRFYIAPKRGGRYVIGATMKPHTFNKSVQPKSIISILERAYSILPALKDAEW
HHHCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
ETAWAGLRPQSNHDVPYMGEHEEIKGLYACTGHYRNGILLSPISGQYMADIIEGKQTNHL
HHHHCCCCCCCCCCCCCCCCHHHHCEEEEEECCCCCCEEECCCCCHHHHHHHCCCCHHHH
LDSLFSKSV
HHHHHHCCC
>Mature Secondary Structure 
SKKYDVAIIGGGVIGSSVAHFLAERSCHVAIVEKQRVASEASKAAAGLLGVQAEWDVYN
CCCEEEEEEECCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHEECCCCCCHHH
PLFELARESRAIFPQLAKALREKTGIDIGYEEKGIYRIAQNEEEKTRIRNIMNWQQETGE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCC
ESYFLTGDELRQKEPFLSSSIIGAVYYPKDGHVIAPELTKAFAHSAAISGTDIYEQTEVF
CCEEECCHHHHHCCCCHHCCCEEEEEECCCCCEECHHHHHHHHHHHCCCCCCCCCCCEEE
DIRVEKNRVCGIVTSEGFISCEKVVIAGGSWSTKLLQYFHRDWGTYPVKGEVVAVKSYKP
EEEEECCCEEEEEECCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCC
LVRAPIFQDRFYIAPKRGGRYVIGATMKPHTFNKSVQPKSIISILERAYSILPALKDAEW
HHHCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
ETAWAGLRPQSNHDVPYMGEHEEIKGLYACTGHYRNGILLSPISGQYMADIIEGKQTNHL
HHHHCCCCCCCCCCCCCCCCHHHHCEEEEEECCCCCCEEECCCCCHHHHHHHCCCCHHHH
LDSLFSKSV
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 9827558; 11744710 [H]