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The map label for this gene is tenA [H]

Identifier: 152974437

GI number: 152974437

Start: 703994

End: 704689

Strand: Direct

Name: tenA [H]

Synonym: Bcer98_0612

Alternate gene names: 152974437

Gene position: 703994-704689 (Clockwise)

Preceding gene: 152974434

Following gene: 152974438

Centisome position: 17.23

GC content: 36.21

Gene sequence:

>696_bases
ATGAAATTTTGTGAAAGATTATTTGAAGTCGTGCAGCCTGTTTGGGAAAAAAGTCATAATCATCCTTTTGTAACGGGGAT
GGGAGATGGCACGTTAGAAAAGGATAAGTTCCAATATTACATCATACAAGATTATTTATACTTGCTAGATTATGCAAAAA
TTTATGCAATTGGTGTTGTAAAAGCAACGAATCCACAAGTTATGGCAAAATTTGCGGAACAAATTGATGGCATTTTAAAT
GGAGAAATGACCATTCATAAGCAGTATGCAAAGCGTCTTGGAATTTCTATAAAAGAGATCGAATCAGCGAAGCCATCAGC
TAAAAATTTAGCGTACACAAACTATATGATGTCAGTATCGCAAAATGGCACACTAGCTGAGCTTATTGCAGCGCTTCTTC
CGTGTATGTGGAGTTATTGGGAAATTGGAAAACGTTTAAATGATATTCCAGGAGCAAGAGATCATGAGTTCTTTGGAGAG
TGGATTCAAGGATATAGTTCAGAAGAGTATGGGAATCTTTGCATTTGGTTAATCGATTTATTAAACGAACTAGCTGAAGG
AAAAACAGAACAAGAATTAGCCCGCTTAGAAGAGATTTTCCTATATTCCAGCCGCTTTGAATATTTATTCTGGGATATGG
CATATAGCAAGGAGATGTGGGGATTTGAAGACAAAGAGCATACTACAGTTTCATAA

Upstream 100 bases:

>100_bases
TGGAGCAATGCATATATATTTTTTTATTTGCTGTAGCCACTTCTTATATAGGAGTGGCTTTTTATTTGGATAAAAAGATA
TAAAGGAAGGGGAAATATAT

Downstream 100 bases:

>100_bases
TGTTTCCTTTCATTATGATGAAAATCCTATTATAAAGGACCTAGATGCTTCCATAAAGGAAAAAGAATTTGTTAGCATCA
TCGGTCCGAGTGGCTGTGGC

Product: TenA family transcription regulator

Products: NA

Alternate protein names: Thiaminase II; Transcriptional activator tenA [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MKFCERLFEVVQPVWEKSHNHPFVTGMGDGTLEKDKFQYYIIQDYLYLLDYAKIYAIGVVKATNPQVMAKFAEQIDGILN
GEMTIHKQYAKRLGISIKEIESAKPSAKNLAYTNYMMSVSQNGTLAELIAALLPCMWSYWEIGKRLNDIPGARDHEFFGE
WIQGYSSEEYGNLCIWLIDLLNELAEGKTEQELARLEEIFLYSSRFEYLFWDMAYSKEMWGFEDKEHTTVS

Sequences:

>Translated_231_residues
MKFCERLFEVVQPVWEKSHNHPFVTGMGDGTLEKDKFQYYIIQDYLYLLDYAKIYAIGVVKATNPQVMAKFAEQIDGILN
GEMTIHKQYAKRLGISIKEIESAKPSAKNLAYTNYMMSVSQNGTLAELIAALLPCMWSYWEIGKRLNDIPGARDHEFFGE
WIQGYSSEEYGNLCIWLIDLLNELAEGKTEQELARLEEIFLYSSRFEYLFWDMAYSKEMWGFEDKEHTTVS
>Mature_231_residues
MKFCERLFEVVQPVWEKSHNHPFVTGMGDGTLEKDKFQYYIIQDYLYLLDYAKIYAIGVVKATNPQVMAKFAEQIDGILN
GEMTIHKQYAKRLGISIKEIESAKPSAKNLAYTNYMMSVSQNGTLAELIAALLPCMWSYWEIGKRLNDIPGARDHEFFGE
WIQGYSSEEYGNLCIWLIDLLNELAEGKTEQELARLEEIFLYSSRFEYLFWDMAYSKEMWGFEDKEHTTVS

Specific function: Stimulates the production of several extracellular degradative enzymes at the transcriptional level, probably by interfering with degS and degU, which are required for this effect. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324517, Length=206, Percent_Identity=32.5242718446602, Blast_Score=99, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6324997, Length=208, Percent_Identity=30.7692307692308, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6325378, Length=206, Percent_Identity=28.6407766990291, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: =3.5.99.2 [H]

Molecular weight: Translated: 26905; Mature: 26905

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFCERLFEVVQPVWEKSHNHPFVTGMGDGTLEKDKFQYYIIQDYLYLLDYAKIYAIGVV
CHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEE
KATNPQVMAKFAEQIDGILNGEMTIHKQYAKRLGISIKEIESAKPSAKNLAYTNYMMSVS
ECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHCC
QNGTLAELIAALLPCMWSYWEIGKRLNDIPGARDHEFFGEWIQGYSSEEYGNLCIWLIDL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHH
LNELAEGKTEQELARLEEIFLYSSRFEYLFWDMAYSKEMWGFEDKEHTTVS
HHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKFCERLFEVVQPVWEKSHNHPFVTGMGDGTLEKDKFQYYIIQDYLYLLDYAKIYAIGVV
CHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEE
KATNPQVMAKFAEQIDGILNGEMTIHKQYAKRLGISIKEIESAKPSAKNLAYTNYMMSVS
ECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHCC
QNGTLAELIAALLPCMWSYWEIGKRLNDIPGARDHEFFGEWIQGYSSEEYGNLCIWLIDL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHH
LNELAEGKTEQELARLEEIFLYSSRFEYLFWDMAYSKEMWGFEDKEHTTVS
HHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]