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The map label for this gene is rbsB [H]

Identifier: 152974391

GI number: 152974391

Start: 644177

End: 645100

Strand: Direct

Name: rbsB [H]

Synonym: Bcer98_0564

Alternate gene names: 152974391

Gene position: 644177-645100 (Clockwise)

Preceding gene: 152974390

Following gene: 152974392

Centisome position: 15.76

GC content: 40.91

Gene sequence:

>924_bases
ATGAAAAAATGGTTACTTGTACTTGTTGCACTCATTATGGTCGTTACTACTGGTTGCTCGATGGAACCACCAGAATGGGC
AAAGGATTCTAGCGATAAAGGGCGAAATAAGACTATTAAAGTTGGATTCTCTGTTTCTACCTTAAATAATCCATTTTTTG
TTACATTGAAAAAAGGAGCAGAAAAGAAAGCGAAGGATATTGGCATAGAATTAATTTCAGTTGATGCACAAAATGATGCA
GCTAAGCAAACGAACGATGTCGAAGACTTGGTTCAAAAAGGTGTTGATATAGTTGTTATTAACCCAACGGATTCAGATGC
TGTTGCTTCAGCGGTAAGTGCAGCCAATGCAGCAAATATTCCTGTAATTACAGTGGACCGCGTTGCAAATTCAGGAAAAG
TTGTCTCTCATATTGCTTCTGATAATGTGGCAGGAGGGAAATTAGCTGGAGATTATATTCGTGAGCTCGTTGGTGAGGGA
GCTGCTGTGGCTGAATTAGAAGGAATTCCGGGATCATCTGCCGCACGCGAGAGAGGGAAAGGATTTCATGAAGTGGCAGA
CACTGGATTGAAAGTAGCAGCTAAGCAAACTGCTGATTTTGACCGTGCGAAAGGATTATCTGTTATGGAAAATATTTTGC
AGGCCAATAGTGATGTGAAGGCAGTATTCGCTCATAATGATGAGATGGCATTAGGTGCGCTAGAAGCGTTAAAATCTGCA
GGCAAAATGGATGTAGCTGTCGTTGGCTTTGATGCAACAGATGATGCCGTGAAAGCGGTTAAAGAAGGACGTATGGCAGC
AACCGTCGCACAAAAGCCAGAATTAATCGGAGAAAAGGCGATGGAAACAGCAAAACAAGTAAATCAAGGTAAAAAAGTGG
AGAAATTCATTCCGATTGGATTGGATCTTATTAAGAAAAAATAA

Upstream 100 bases:

>100_bases
TAGGCGTGTCATCTTTCTTCCAACAAGTTGTAAAAGGGCTTGTTATCCTACTTGCTGTATTAATTGATCGTCGAAAAGAA
GCGTAATGGAGGGATAATTC

Downstream 100 bases:

>100_bases
AATATGAAAAATAAGGAAGGAAGAATTACAAATGAAATTCTTTATTGATACAGCAAATATTAATGAAATTAAAGAAGCAA
ATGAATTAGGTATATTAGCA

Product: monosaccharide-transporting ATPase

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MKKWLLVLVALIMVVTTGCSMEPPEWAKDSSDKGRNKTIKVGFSVSTLNNPFFVTLKKGAEKKAKDIGIELISVDAQNDA
AKQTNDVEDLVQKGVDIVVINPTDSDAVASAVSAANAANIPVITVDRVANSGKVVSHIASDNVAGGKLAGDYIRELVGEG
AAVAELEGIPGSSAARERGKGFHEVADTGLKVAAKQTADFDRAKGLSVMENILQANSDVKAVFAHNDEMALGALEALKSA
GKMDVAVVGFDATDDAVKAVKEGRMAATVAQKPELIGEKAMETAKQVNQGKKVEKFIPIGLDLIKKK

Sequences:

>Translated_307_residues
MKKWLLVLVALIMVVTTGCSMEPPEWAKDSSDKGRNKTIKVGFSVSTLNNPFFVTLKKGAEKKAKDIGIELISVDAQNDA
AKQTNDVEDLVQKGVDIVVINPTDSDAVASAVSAANAANIPVITVDRVANSGKVVSHIASDNVAGGKLAGDYIRELVGEG
AAVAELEGIPGSSAARERGKGFHEVADTGLKVAAKQTADFDRAKGLSVMENILQANSDVKAVFAHNDEMALGALEALKSA
GKMDVAVVGFDATDDAVKAVKEGRMAATVAQKPELIGEKAMETAKQVNQGKKVEKFIPIGLDLIKKK
>Mature_307_residues
MKKWLLVLVALIMVVTTGCSMEPPEWAKDSSDKGRNKTIKVGFSVSTLNNPFFVTLKKGAEKKAKDIGIELISVDAQNDA
AKQTNDVEDLVQKGVDIVVINPTDSDAVASAVSAANAANIPVITVDRVANSGKVVSHIASDNVAGGKLAGDYIRELVGEG
AAVAELEGIPGSSAARERGKGFHEVADTGLKVAAKQTADFDRAKGLSVMENILQANSDVKAVFAHNDEMALGALEALKSA
GKMDVAVVGFDATDDAVKAVKEGRMAATVAQKPELIGEKAMETAKQVNQGKKVEKFIPIGLDLIKKK

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=308, Percent_Identity=54.8701298701299, Blast_Score=311, Evalue=3e-86,
Organism=Escherichia coli, GI1790526, Length=268, Percent_Identity=35.0746268656716, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1790674, Length=251, Percent_Identity=32.6693227091633, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788473, Length=274, Percent_Identity=29.1970802919708, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790194, Length=224, Percent_Identity=27.6785714285714, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789990, Length=264, Percent_Identity=28.030303030303, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1786540, Length=228, Percent_Identity=28.5087719298246, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32222; Mature: 32222

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWLLVLVALIMVVTTGCSMEPPEWAKDSSDKGRNKTIKVGFSVSTLNNPFFVTLKKGA
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCEEEEECCCC
EKKAKDIGIELISVDAQNDAAKQTNDVEDLVQKGVDIVVINPTDSDAVASAVSAANAANI
CHHHHHCCEEEEEEECCCCHHHCCCCHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCC
PVITVDRVANSGKVVSHIASDNVAGGKLAGDYIRELVGEGAAVAELEGIPGSSAARERGK
CEEECCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCC
GFHEVADTGLKVAAKQTADFDRAKGLSVMENILQANSDVKAVFAHNDEMALGALEALKSA
CHHHHHHCCHHHHHHHHCCHHHHCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCC
GKMDVAVVGFDATDDAVKAVKEGRMAATVAQKPELIGEKAMETAKQVNQGKKVEKFIPIG
CCCEEEEEECCCCHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHCCC
LDLIKKK
HHHHCCC
>Mature Secondary Structure
MKKWLLVLVALIMVVTTGCSMEPPEWAKDSSDKGRNKTIKVGFSVSTLNNPFFVTLKKGA
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCEEEEECCCC
EKKAKDIGIELISVDAQNDAAKQTNDVEDLVQKGVDIVVINPTDSDAVASAVSAANAANI
CHHHHHCCEEEEEEECCCCHHHCCCCHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCC
PVITVDRVANSGKVVSHIASDNVAGGKLAGDYIRELVGEGAAVAELEGIPGSSAARERGK
CEEECCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCC
GFHEVADTGLKVAAKQTADFDRAKGLSVMENILQANSDVKAVFAHNDEMALGALEALKSA
CHHHHHHCCHHHHHHHHCCHHHHCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCC
GKMDVAVVGFDATDDAVKAVKEGRMAATVAQKPELIGEKAMETAKQVNQGKKVEKFIPIG
CCCEEEEEECCCCHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHCCC
LDLIKKK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]