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The map label for this gene is ytsJ [H]

Identifier: 152974330

GI number: 152974330

Start: 565760

End: 566965

Strand: Direct

Name: ytsJ [H]

Synonym: Bcer98_0499

Alternate gene names: 152974330

Gene position: 565760-566965 (Clockwise)

Preceding gene: 152974329

Following gene: 152974336

Centisome position: 13.84

GC content: 38.31

Gene sequence:

>1206_bases
ATGTTAGAAAAGCAAATTAATGAGCGATCATTATTACTTCATAAAGAGTTAGTAGGAAAGATTGAAATTACGAGTAAAGT
AGAAGTAAATTCAGCAGAGGATTTAAGTTTGACATATACACCAGGAGTAGCGGAGTCTTGTAAAGCTATCGCGGCCGATG
AAGAGACAGCGTATGATTACACAGCACGTGGTAATATGGTGGCGGTTGTATCCGATGGGACAGCTGTACTTGGTTTAGGT
GATATTGGACCGAAAGCTGCGATGCCTGTTATGGAAGGAAAGTGTATTTTATTTAAGAAATTCGCGAACGTAGATGCATT
TCCGTTATGTTTGGGAACAAAAGATGTAGATGAAATCGTTACCCTTGTGAAAAATTTAGAGCCGACATTTGCTGGAATCA
ATTTAGAAGATATTGCAGCACCGCGTTGCTTTGAAATTGAAAAACGTCTAAAAGAGGAAACAAATATTCCAGTTTTTCAT
GATGATCAGCATGGAACGGCAATTGTCGTGTTAGCTGCTGTAATTAATGCATTAAAAGTTGTAAAGAAGCAAATGAATAC
AGTGAAAATTGTCATTAATGGTGCGGGATCAGCGGGAATAGCAATTGGTAACTTGTTATTAAAAGCCGGAGTAGAACATA
TAACATTAGTAAGTTTAGAAGGCATTGTTTGTGAGGGAGAATTATGGATGAATCCAGAACAAATAGAAATTGCGAAGAAA
ACAAATCGAGAACATGTGCGAGGATCATTAAAAGAGGCAATTCGTGAGGCGGATATTTTTATCGGTGTATCTGCTCCAAA
TGTATTAACAAAAGAGCTTGTACAGGCGATGAATAAAAAGCCAATTGTATTTGCGATGGCAAATCCAATTCCAGAGATAT
TTCCAGATGAAGCATTAGCAGCCGGAGCAGCTGTTGTTGGCACAGGAAGATCTGATTATCCAAATCAGGTAAATAATGTA
TTGGCGTTTCCCGGTATCTTCCGAGGGGCATTAGATGTACGTGCAACGGATGTTACAGAAGAAATGAAATTAGCAGCAGC
ATATGGAATTGCTAACATTATTACGGATGAAGAAAGAAATGAAAACTATGTTATCCCAAATCCGCTTGATAAAAGAGTTG
TGCCAAGTGTGGCAGCGGCAGTAGCAAAAGCAGCAATTGAGTCAGGAGTAGCGCAAATTAAAAAAATACCAAGTTATGAA
TGCTAA

Upstream 100 bases:

>100_bases
CCGCAACAATTTTATTAAAAATGTTTTCTTAAATAACAATCGGGCAAGCTAGCTATAGAAAGATATGGCTAGCTTGTCAA
ATTGAGGGGAGAATATACCG

Downstream 100 bases:

>100_bases
AAAGGAGCCTTCTTACGAAAGAAGTCTCCTTTTTAGCAGATAAGAAATCTTTAATACAAGGAATAAGTTGTTATTCTTTA
TAGCATTATGAATTTTACTT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME 4 [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MLEKQINERSLLLHKELVGKIEITSKVEVNSAEDLSLTYTPGVAESCKAIAADEETAYDYTARGNMVAVVSDGTAVLGLG
DIGPKAAMPVMEGKCILFKKFANVDAFPLCLGTKDVDEIVTLVKNLEPTFAGINLEDIAAPRCFEIEKRLKEETNIPVFH
DDQHGTAIVVLAAVINALKVVKKQMNTVKIVINGAGSAGIAIGNLLLKAGVEHITLVSLEGIVCEGELWMNPEQIEIAKK
TNREHVRGSLKEAIREADIFIGVSAPNVLTKELVQAMNKKPIVFAMANPIPEIFPDEALAAGAAVVGTGRSDYPNQVNNV
LAFPGIFRGALDVRATDVTEEMKLAAAYGIANIITDEERNENYVIPNPLDKRVVPSVAAAVAKAAIESGVAQIKKIPSYE
C

Sequences:

>Translated_401_residues
MLEKQINERSLLLHKELVGKIEITSKVEVNSAEDLSLTYTPGVAESCKAIAADEETAYDYTARGNMVAVVSDGTAVLGLG
DIGPKAAMPVMEGKCILFKKFANVDAFPLCLGTKDVDEIVTLVKNLEPTFAGINLEDIAAPRCFEIEKRLKEETNIPVFH
DDQHGTAIVVLAAVINALKVVKKQMNTVKIVINGAGSAGIAIGNLLLKAGVEHITLVSLEGIVCEGELWMNPEQIEIAKK
TNREHVRGSLKEAIREADIFIGVSAPNVLTKELVQAMNKKPIVFAMANPIPEIFPDEALAAGAAVVGTGRSDYPNQVNNV
LAFPGIFRGALDVRATDVTEEMKLAAAYGIANIITDEERNENYVIPNPLDKRVVPSVAAAVAKAAIESGVAQIKKIPSYE
C
>Mature_401_residues
MLEKQINERSLLLHKELVGKIEITSKVEVNSAEDLSLTYTPGVAESCKAIAADEETAYDYTARGNMVAVVSDGTAVLGLG
DIGPKAAMPVMEGKCILFKKFANVDAFPLCLGTKDVDEIVTLVKNLEPTFAGINLEDIAAPRCFEIEKRLKEETNIPVFH
DDQHGTAIVVLAAVINALKVVKKQMNTVKIVINGAGSAGIAIGNLLLKAGVEHITLVSLEGIVCEGELWMNPEQIEIAKK
TNREHVRGSLKEAIREADIFIGVSAPNVLTKELVQAMNKKPIVFAMANPIPEIFPDEALAAGAAVVGTGRSDYPNQVNNV
LAFPGIFRGALDVRATDVTEEMKLAAAYGIANIITDEERNENYVIPNPLDKRVVPSVAAAVAKAAIESGVAQIKKIPSYE
C

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=394, Percent_Identity=24.6192893401015, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI62420882, Length=426, Percent_Identity=24.4131455399061, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI62420880, Length=426, Percent_Identity=24.4131455399061, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI239049447, Length=426, Percent_Identity=24.4131455399061, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI4505145, Length=359, Percent_Identity=25.9052924791086, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI270265879, Length=318, Percent_Identity=26.7295597484277, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788806, Length=409, Percent_Identity=48.1662591687042, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI87081919, Length=447, Percent_Identity=27.2930648769575, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17537199, Length=458, Percent_Identity=23.3624454148472, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322823, Length=392, Percent_Identity=26.7857142857143, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI78707242, Length=439, Percent_Identity=26.1958997722096, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI78707238, Length=439, Percent_Identity=26.1958997722096, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI78707240, Length=446, Percent_Identity=26.0089686098655, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI281362674, Length=448, Percent_Identity=25.2232142857143, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI281362672, Length=448, Percent_Identity=25.2232142857143, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24646386, Length=448, Percent_Identity=25.2232142857143, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24646388, Length=448, Percent_Identity=25.2232142857143, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI21356279, Length=448, Percent_Identity=25.2232142857143, Blast_Score=95, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 43009; Mature: 43009

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEKQINERSLLLHKELVGKIEITSKVEVNSAEDLSLTYTPGVAESCKAIAADEETAYDY
CCCCCCCHHHHHHHHHHHCEEEEEEEEECCCCCCCEEEECCCHHHHHHHHCCCCCCCEEE
TARGNMVAVVSDGTAVLGLGDIGPKAAMPVMEGKCILFKKFANVDAFPLCLGTKDVDEIV
ECCCCEEEEEECCEEEEEECCCCCHHCCCCCCCCEEEEEECCCCCCEEEEECCCCHHHHH
TLVKNLEPTFAGINLEDIAAPRCFEIEKRLKEETNIPVFHDDQHGTAIVVLAAVINALKV
HHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHH
VKKQMNTVKIVINGAGSAGIAIGNLLLKAGVEHITLVSLEGIVCEGELWMNPEQIEIAKK
HHHHCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCEEECCCEECCHHHHHHHHH
TNREHVRGSLKEAIREADIFIGVSAPNVLTKELVQAMNKKPIVFAMANPIPEIFPDEALA
CCHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHH
AGAAVVGTGRSDYPNQVNNVLAFPGIFRGALDVRATDVTEEMKLAAAYGIANIITDEERN
CCCEEEECCCCCCCHHHHCEEECCHHHHCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCC
ENYVIPNPLDKRVVPSVAAAVAKAAIESGVAQIKKIPSYEC
CCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLEKQINERSLLLHKELVGKIEITSKVEVNSAEDLSLTYTPGVAESCKAIAADEETAYDY
CCCCCCCHHHHHHHHHHHCEEEEEEEEECCCCCCCEEEECCCHHHHHHHHCCCCCCCEEE
TARGNMVAVVSDGTAVLGLGDIGPKAAMPVMEGKCILFKKFANVDAFPLCLGTKDVDEIV
ECCCCEEEEEECCEEEEEECCCCCHHCCCCCCCCEEEEEECCCCCCEEEEECCCCHHHHH
TLVKNLEPTFAGINLEDIAAPRCFEIEKRLKEETNIPVFHDDQHGTAIVVLAAVINALKV
HHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHH
VKKQMNTVKIVINGAGSAGIAIGNLLLKAGVEHITLVSLEGIVCEGELWMNPEQIEIAKK
HHHHCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCEEECCCEECCHHHHHHHHH
TNREHVRGSLKEAIREADIFIGVSAPNVLTKELVQAMNKKPIVFAMANPIPEIFPDEALA
CCHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHH
AGAAVVGTGRSDYPNQVNNVLAFPGIFRGALDVRATDVTEEMKLAAAYGIANIITDEERN
CCCEEEECCCCCCCHHHHCEEECCHHHHCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCC
ENYVIPNPLDKRVVPSVAAAVAKAAIESGVAQIKKIPSYEC
CCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]