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The map label for this gene is nagP [H]

Identifier: 152974260

GI number: 152974260

Start: 480514

End: 482007

Strand: Direct

Name: nagP [H]

Synonym: Bcer98_0427

Alternate gene names: 152974260

Gene position: 480514-482007 (Clockwise)

Preceding gene: 152974257

Following gene: 152974261

Centisome position: 11.76

GC content: 37.28

Gene sequence:

>1494_bases
ATGTTACAGTTTTTACAACGTATTGGTAAAGCATTAATGCTTCCAATTGCAGTATTGCCAGCAGCAGGATTATTGCTTCG
TTTAGGGCAACCAGATGTATTTGATATACCTGTTATGGCACAAGCCGGTGCAGCTATTTTTGATAACTTAGCACTTATTT
TTGCAATTGGCGTTGCAATCGGTTTGTCAGTTGATGGTAGTGGTGCTGCAGGTCTTGCAGGTGCGATTGGTTATCTAGTT
ATGAAGAATACAACTAATACTTTAAGTGTTAGTTATTCGACAGCAGAACTAAATGATAAATTAAAAAGTGTTCAAGACTT
ATTGGGGTCAGTTGATCCAAGTAAGTTAGCGGATACAATGACAAAAGTTTCAAAAGCAGCAGCGTTAACACCTAAAATAA
ATATGGCGATCCTTGGTGGGATTATTGCCGGGATTGTTGCTGGGTTATTATATAACAAATTCCATAAGATCAAATTGCCA
GAATGGTTAGGTTTCTTTGCAGGAAAACGCTTTGTTCCAATTATTACATCCGTCGTTATGCTTCTTTTAGGATTAGTATT
TGGTCAAATTTGGCCAACAATTCAAAGTGGCATTGATGCAGTAGCTCATGGTATTGTCAACTTAGGTTCAATTGGATCTG
GTTTATTCGGTTTATTGAACCGTTTATTGATTCCAATTGGCTTGCACCATGTAATGAACACATACTTCTGGTTTGTGCTT
GGTGACTTTACAAATGCAGCAGGCGATATTGTTCATGGTGATATTGCTCGTTTCTTTGCGAAAGATCCATCAGCAGGTAT
GTTTATGACTGGTTTCTTCCCAGTTATGATGTTTGGTTTACCAGCAGCATGTTTCGCAATGATTGCAGCTGCGAAACCAG
AAAAACGTAAAATGGTTACAGGAATGTTAGCGGGTCTTGCACTAACTTCATTTTTAACTGGTATTACAGAACCAATTGAA
TTCTCGTTCATGTTCTTATCTCCAGTATTATATGGTATCCATGCTGTATTAACAGGTATTTCACTATTTGTAACAACATC
ACTTGGCATTCATGATGGTTTTTCATTTAGTGCTGGTGCCATTGATTATTTCTTAAACTTTGGTATTGCAACAAAACCAT
TATTATTAGCAGGAATTGGATTAATTTATGCAGCTATTTACTTTGTTGTATTCTACTTCTTAATTAAGAAATTTAACTTG
AAAACTCCAGGGCGTGAAGATGATGAGGAATTAGCAACAGATGATGAAGCGCCAGTTGCAGGTTCGATTGGAGAAGCATA
TGTAACTGCACTCGGCGGAAAAGACAACTTAACAGTGATTGATAACTGTGCGACTCGTCTCCGTCTACAAGTAAAAGATG
CAAACTTAGTAAATGAATCAGCGTTAAAACGTGCTGGTGCGAGAGGTGTTATGAAATTAAGTAACACAAGTGTGCAAGTT
ATCGTTGGAACAAACGTTGAATCAGTTGCTGAAGATATGAAAAAACACGTATAA

Upstream 100 bases:

>100_bases
TCTATACAACTGTATGTTATGATGGTTTCAAAAGATAAAGCGTTTTCTTAAATATGTATATAATGGTAACGATTTCAGAA
ATTGGATAGGGGGAATCATA

Downstream 100 bases:

>100_bases
ATTGAAGAGAAAAGAGGATATCCGAAAAGATGGATAAAATGCATCTTTTAGAGGATATCCTCTTTCTTATATGTGAATCT
TACTTTTGAAAAATTTCTTT

Product: PTS system, N-acetylglucosamine-specific IIBC subunit

Products: NA

Alternate protein names: EIICB-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MLQFLQRIGKALMLPIAVLPAAGLLLRLGQPDVFDIPVMAQAGAAIFDNLALIFAIGVAIGLSVDGSGAAGLAGAIGYLV
MKNTTNTLSVSYSTAELNDKLKSVQDLLGSVDPSKLADTMTKVSKAAALTPKINMAILGGIIAGIVAGLLYNKFHKIKLP
EWLGFFAGKRFVPIITSVVMLLLGLVFGQIWPTIQSGIDAVAHGIVNLGSIGSGLFGLLNRLLIPIGLHHVMNTYFWFVL
GDFTNAAGDIVHGDIARFFAKDPSAGMFMTGFFPVMMFGLPAACFAMIAAAKPEKRKMVTGMLAGLALTSFLTGITEPIE
FSFMFLSPVLYGIHAVLTGISLFVTTSLGIHDGFSFSAGAIDYFLNFGIATKPLLLAGIGLIYAAIYFVVFYFLIKKFNL
KTPGREDDEELATDDEAPVAGSIGEAYVTALGGKDNLTVIDNCATRLRLQVKDANLVNESALKRAGARGVMKLSNTSVQV
IVGTNVESVAEDMKKHV

Sequences:

>Translated_497_residues
MLQFLQRIGKALMLPIAVLPAAGLLLRLGQPDVFDIPVMAQAGAAIFDNLALIFAIGVAIGLSVDGSGAAGLAGAIGYLV
MKNTTNTLSVSYSTAELNDKLKSVQDLLGSVDPSKLADTMTKVSKAAALTPKINMAILGGIIAGIVAGLLYNKFHKIKLP
EWLGFFAGKRFVPIITSVVMLLLGLVFGQIWPTIQSGIDAVAHGIVNLGSIGSGLFGLLNRLLIPIGLHHVMNTYFWFVL
GDFTNAAGDIVHGDIARFFAKDPSAGMFMTGFFPVMMFGLPAACFAMIAAAKPEKRKMVTGMLAGLALTSFLTGITEPIE
FSFMFLSPVLYGIHAVLTGISLFVTTSLGIHDGFSFSAGAIDYFLNFGIATKPLLLAGIGLIYAAIYFVVFYFLIKKFNL
KTPGREDDEELATDDEAPVAGSIGEAYVTALGGKDNLTVIDNCATRLRLQVKDANLVNESALKRAGARGVMKLSNTSVQV
IVGTNVESVAEDMKKHV
>Mature_497_residues
MLQFLQRIGKALMLPIAVLPAAGLLLRLGQPDVFDIPVMAQAGAAIFDNLALIFAIGVAIGLSVDGSGAAGLAGAIGYLV
MKNTTNTLSVSYSTAELNDKLKSVQDLLGSVDPSKLADTMTKVSKAAALTPKINMAILGGIIAGIVAGLLYNKFHKIKLP
EWLGFFAGKRFVPIITSVVMLLLGLVFGQIWPTIQSGIDAVAHGIVNLGSIGSGLFGLLNRLLIPIGLHHVMNTYFWFVL
GDFTNAAGDIVHGDIARFFAKDPSAGMFMTGFFPVMMFGLPAACFAMIAAAKPEKRKMVTGMLAGLALTSFLTGITEPIE
FSFMFLSPVLYGIHAVLTGISLFVTTSLGIHDGFSFSAGAIDYFLNFGIATKPLLLAGIGLIYAAIYFVVFYFLIKKFNL
KTPGREDDEELATDDEAPVAGSIGEAYVTALGGKDNLTVIDNCATRLRLQVKDANLVNESALKRAGARGVMKLSNTSVQV
IVGTNVESVAEDMKKHV

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=505, Percent_Identity=52.2772277227723, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1787343, Length=501, Percent_Identity=42.315369261477, Blast_Score=377, Evalue=1e-105,
Organism=Escherichia coli, GI1787908, Length=535, Percent_Identity=37.5700934579439, Blast_Score=306, Evalue=2e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 52693; Mature: 52693

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EECCCHHHHHHHHHHCC
>Mature Secondary Structure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EECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]