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The map label for this gene is fumA [H]

Identifier: 152974243

GI number: 152974243

Start: 464966

End: 466546

Strand: Direct

Name: fumA [H]

Synonym: Bcer98_0409

Alternate gene names: 152974243

Gene position: 464966-466546 (Clockwise)

Preceding gene: 152974242

Following gene: 152974244

Centisome position: 11.38

GC content: 39.85

Gene sequence:

>1581_bases
ATGAAGTTTTCATCTAACGACGTTTTGGTAGCGTTCACAATTGAGGGGGAAGTAACAATGGAAAAGCTTCAAGAAAGCAT
GTATCAACTTATTGTTGAAACGTCGACGAATTTACCAAAGGATGTTCGTCGTGCAATTCAACAAGCAAAGGAACGAGAGA
ATGCAGGGACTCGTTCCGCGATGGCACTTGGCACAATTACAAAGAATATTAAAATGGCAGATGACAACATTTCGCCAATT
TGCCAAGATACAGGGATGCCAACATTTAAAATCTATACACCAGTTGGTGTGAATCAATTAAAAATGAAAGAAGCTATCTA
TAGTGCGCTTGAGCAGGCGACCAAGGATGGAAAATTACGTCCGAACTCTGTCGATTCCCTTTTTGGAGACAACAGTGGAA
ACAACTTAGGGCCCGGAACACCTGTTATCAAATTTGAACAGTGGGAAAAAGATTATATCGATGCTCGTTTAATTTTAAAA
GGAGGCGGCTGTGAAAATAAAAATATTCAATATAGCTTGCCTTGCGAACTAGAAGGGCTTAGACGAGCAGGACGTGATTT
AGAAGGGATTCGTAAATGTCTTCTTCATGCGGTATATCAAGCACAAGGACAAGGATGTAGTGCAGGTGTAATTGGTGTCG
GTATCGGGGGAGACCGCACGTCGGGATATGAATTAGCTAAAAATCAATTATTCCGTCCATTAGATGATGTGAATCCAATT
CCAGAATTGCAGAAATTAGAAGAATATGTATTAGAGAATGCTAATAAACTTGGGATTGGTACAATGGGATTTGGTGGAGA
AACGACTTTACTTGGTTGTAAAATTGGAGTATACAATCGTTTGCCAGCGAGCTTCTATGTGTCTGTAGCATATAATTGTT
GGGCATATCGCCGTCTTGGTGTAACACTTCACCCAGCAACAGGTGAAATTATCGAGTGGTTATATCAAGAAGGAGAAGAT
ACGCTTCAACAAGAGGTTGTACAAGAAAAACAAGAACAACGTGAAATCGTATTACAAGCCCCAATTACAGAAGAACAAAT
TCGTGAACTGCGCGTTGGGGATGTTGTAACAATTAACGGTATGATGTACACAGGTCGTGATGCGATTCATAAACATTTAA
TGGATCATGACTGCCCTGTAGATTTAAATGGTCAAATCATCTATCATTGTGGGCCAGTTGTAGTGAAAGATGAAAATGAG
AACTGGAAAATTAAAGCGGCAGGCCCAACAACAAGTATCCGTGAAGAACCATATCAAGGAGATATTATGAAGAAATTCGG
TATCCGTGCAGTCATTGGTAAAGGTGGTATGGGTGCAAAGACATTAGCTGCACTGAAAGAACATGGCGGTGTCTATTTAA
ATGCAATTGGCGGTGCTGCGCAGTATTATGCCGAATGTATTAAAGAAGTAAAAGATGTAGATTTTCTACAGTTTGGTATT
CCAGAAGCAATGTGGCATTTACGCATTGATGGTTTTAAAGCAGTTGTAACAATGGATTCTCATGGTAATAGCTTGCATGC
TGACGTGGATAAAACATCACTTGAAAAGTTGGCGGAGTTCAAAGAACCGGTATTTAAATAA

Upstream 100 bases:

>100_bases
TTTTATCAAATAGAATGAATATAAAAAAAAGGGTATAATCAAGTTGTGAAAAATTTCATTTTACTTTCAGATGATAGACA
TACAAACAAGGAAGGATAGA

Downstream 100 bases:

>100_bases
AGAAAGAAAAAAATGCATCTCGAGTTACTGGAGATGCATTTTTTGGTATATGTAGAAAAATATGCTCAGCATGAAAGGAA
GATTAAAAACAGAAACTATC

Product: tartrate/fumarate subfamily Fe-S type hydro-lyase alpha subunit

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MKFSSNDVLVAFTIEGEVTMEKLQESMYQLIVETSTNLPKDVRRAIQQAKERENAGTRSAMALGTITKNIKMADDNISPI
CQDTGMPTFKIYTPVGVNQLKMKEAIYSALEQATKDGKLRPNSVDSLFGDNSGNNLGPGTPVIKFEQWEKDYIDARLILK
GGGCENKNIQYSLPCELEGLRRAGRDLEGIRKCLLHAVYQAQGQGCSAGVIGVGIGGDRTSGYELAKNQLFRPLDDVNPI
PELQKLEEYVLENANKLGIGTMGFGGETTLLGCKIGVYNRLPASFYVSVAYNCWAYRRLGVTLHPATGEIIEWLYQEGED
TLQQEVVQEKQEQREIVLQAPITEEQIRELRVGDVVTINGMMYTGRDAIHKHLMDHDCPVDLNGQIIYHCGPVVVKDENE
NWKIKAAGPTTSIREEPYQGDIMKKFGIRAVIGKGGMGAKTLAALKEHGGVYLNAIGGAAQYYAECIKEVKDVDFLQFGI
PEAMWHLRIDGFKAVVTMDSHGNSLHADVDKTSLEKLAEFKEPVFK

Sequences:

>Translated_526_residues
MKFSSNDVLVAFTIEGEVTMEKLQESMYQLIVETSTNLPKDVRRAIQQAKERENAGTRSAMALGTITKNIKMADDNISPI
CQDTGMPTFKIYTPVGVNQLKMKEAIYSALEQATKDGKLRPNSVDSLFGDNSGNNLGPGTPVIKFEQWEKDYIDARLILK
GGGCENKNIQYSLPCELEGLRRAGRDLEGIRKCLLHAVYQAQGQGCSAGVIGVGIGGDRTSGYELAKNQLFRPLDDVNPI
PELQKLEEYVLENANKLGIGTMGFGGETTLLGCKIGVYNRLPASFYVSVAYNCWAYRRLGVTLHPATGEIIEWLYQEGED
TLQQEVVQEKQEQREIVLQAPITEEQIRELRVGDVVTINGMMYTGRDAIHKHLMDHDCPVDLNGQIIYHCGPVVVKDENE
NWKIKAAGPTTSIREEPYQGDIMKKFGIRAVIGKGGMGAKTLAALKEHGGVYLNAIGGAAQYYAECIKEVKDVDFLQFGI
PEAMWHLRIDGFKAVVTMDSHGNSLHADVDKTSLEKLAEFKEPVFK
>Mature_526_residues
MKFSSNDVLVAFTIEGEVTMEKLQESMYQLIVETSTNLPKDVRRAIQQAKERENAGTRSAMALGTITKNIKMADDNISPI
CQDTGMPTFKIYTPVGVNQLKMKEAIYSALEQATKDGKLRPNSVDSLFGDNSGNNLGPGTPVIKFEQWEKDYIDARLILK
GGGCENKNIQYSLPCELEGLRRAGRDLEGIRKCLLHAVYQAQGQGCSAGVIGVGIGGDRTSGYELAKNQLFRPLDDVNPI
PELQKLEEYVLENANKLGIGTMGFGGETTLLGCKIGVYNRLPASFYVSVAYNCWAYRRLGVTLHPATGEIIEWLYQEGED
TLQQEVVQEKQEQREIVLQAPITEEQIRELRVGDVVTINGMMYTGRDAIHKHLMDHDCPVDLNGQIIYHCGPVVVKDENE
NWKIKAAGPTTSIREEPYQGDIMKKFGIRAVIGKGGMGAKTLAALKEHGGVYLNAIGGAAQYYAECIKEVKDVDFLQFGI
PEAMWHLRIDGFKAVVTMDSHGNSLHADVDKTSLEKLAEFKEPVFK

Specific function: Unknown

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1789443, Length=191, Percent_Identity=34.0314136125654, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1789442, Length=287, Percent_Identity=27.8745644599303, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1787897, Length=476, Percent_Identity=25, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1790564, Length=467, Percent_Identity=24.4111349036403, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 58219; Mature: 58219

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSSNDVLVAFTIEGEVTMEKLQESMYQLIVETSTNLPKDVRRAIQQAKERENAGTRSA
CCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHH
MALGTITKNIKMADDNISPICQDTGMPTFKIYTPVGVNQLKMKEAIYSALEQATKDGKLR
HHHHHHHCCCEECCCCCCCHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC
PNSVDSLFGDNSGNNLGPGTPVIKFEQWEKDYIDARLILKGGGCENKNIQYSLPCELEGL
CCCHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCCEEEECCCCHHHH
RRAGRDLEGIRKCLLHAVYQAQGQGCSAGVIGVGIGGDRTSGYELAKNQLFRPLDDVNPI
HHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCHHCCCCC
PELQKLEEYVLENANKLGIGTMGFGGETTLLGCKIGVYNRLPASFYVSVAYNCWAYRRLG
HHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEECCCCCCCHHEEEEEHHHHEEEEEEC
VTLHPATGEIIEWLYQEGEDTLQQEVVQEKQEQREIVLQAPITEEQIRELRVGDVVTING
EEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHCCCCEEEECC
MMYTGRDAIHKHLMDHDCPVDLNGQIIYHCGPVVVKDENENWKIKAAGPTTSIREEPYQG
EEECCHHHHHHHHHCCCCCCCCCCEEEEEECCEEEEECCCCEEEEECCCCCCCCCCCCCC
DIMKKFGIRAVIGKGGMGAKTLAALKEHGGVYLNAIGGAAQYYAECIKEVKDVDFLQFGI
CHHHHCCCEEEECCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCHHHHHCC
PEAMWHLRIDGFKAVVTMDSHGNSLHADVDKTSLEKLAEFKEPVFK
CHHHEEEEECCEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFSSNDVLVAFTIEGEVTMEKLQESMYQLIVETSTNLPKDVRRAIQQAKERENAGTRSA
CCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHH
MALGTITKNIKMADDNISPICQDTGMPTFKIYTPVGVNQLKMKEAIYSALEQATKDGKLR
HHHHHHHCCCEECCCCCCCHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC
PNSVDSLFGDNSGNNLGPGTPVIKFEQWEKDYIDARLILKGGGCENKNIQYSLPCELEGL
CCCHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCCEEEECCCCHHHH
RRAGRDLEGIRKCLLHAVYQAQGQGCSAGVIGVGIGGDRTSGYELAKNQLFRPLDDVNPI
HHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCHHCCCCC
PELQKLEEYVLENANKLGIGTMGFGGETTLLGCKIGVYNRLPASFYVSVAYNCWAYRRLG
HHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEEECCCCCCCHHEEEEEHHHHEEEEEEC
VTLHPATGEIIEWLYQEGEDTLQQEVVQEKQEQREIVLQAPITEEQIRELRVGDVVTING
EEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHCCCCEEEECC
MMYTGRDAIHKHLMDHDCPVDLNGQIIYHCGPVVVKDENENWKIKAAGPTTSIREEPYQG
EEECCHHHHHHHHHCCCCCCCCCCEEEEEECCEEEEECCCCEEEEECCCCCCCCCCCCCC
DIMKKFGIRAVIGKGGMGAKTLAALKEHGGVYLNAIGGAAQYYAECIKEVKDVDFLQFGI
CHHHHCCCEEEECCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCHHHHHCC
PEAMWHLRIDGFKAVVTMDSHGNSLHADVDKTSLEKLAEFKEPVFK
CHHHEEEEECCEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8473853 [H]