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The map label for this gene is ntcA [H]

Identifier: 152974188

GI number: 152974188

Start: 401315

End: 402004

Strand: Direct

Name: ntcA [H]

Synonym: Bcer98_0352

Alternate gene names: 152974188

Gene position: 401315-402004 (Clockwise)

Preceding gene: 152974187

Following gene: 152974189

Centisome position: 9.82

GC content: 31.3

Gene sequence:

>690_bases
ATGAATAGGCGGAATGTGGTTAAAGATTTAAAGCAGTTTGAATTATTTTCTCATGTAACAGACAAAAAGTTAAAAGGTTT
AACGGAATTTATTTATTGGCGCACCTATAAGAAAGGACAATTTTTATTTTTAGAAGGAGATTCAAGAGAACGAATTTACC
TTATGTTAGATGGTTTTGTAAAGTTAGAACGAGTAAATCAAAGTGGAAATTTATTATATGAAGACTATGTAAAGAGATAT
TCTATTTTTCCTTATAGTGGTATGTTTACTGATAAAGGATACAGCTATACGGCAGAAGCTATAACTGATGTCGATGTGTA
TTATATTCCGACAGTAATTTTTGAAGATATGGTGAAGGCAAATCGAACACAATTAATGCACATTGTTCAGCAGTTATCCT
CAATATTAAAGTTGAATGAAAATCGTGTGCAAAACATTACGATTCCTAATGCACAAGATCGTGTTATTCAAACATTAAAT
TATTTAATGCATGACTTAGGCGAACCAAACGGTGAAGAAATTGTAATCCCTTGTCCACTGACAACAATTGAAATTTCTAA
AATATCTGGAACATCTCGAGAGACAGTTAGCAGCGTATTAAAACAATTGAAAAATGACCGTATTGTTACCGTGTTAGGTA
AAAAAATTACAATACATAATCCTACTTATTTTGAAGAAAGCGCTATGTGA

Upstream 100 bases:

>100_bases
ATTATGAAATTAAGAGATGTGGATGGGTTATGATAAAATTTACTTGAATGCGCTTTCTTGATGTTTTAATACTATGTATA
GCAAGAAAGAGGGATTTCTT

Downstream 100 bases:

>100_bases
AAAATACAATAATGAATGTGAGAGAAAAGAAGCATTGAGACATAAAGATGTATCAATGCTTTTATCATTTTAATTGTACA
TGAATGAATATATATTTATG

Product: CRP/FNR family transcriptional regulator

Products: NA

Alternate protein names: DNA-binding protein VF1; Nitrogen-responsive regulatory protein [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MNRRNVVKDLKQFELFSHVTDKKLKGLTEFIYWRTYKKGQFLFLEGDSRERIYLMLDGFVKLERVNQSGNLLYEDYVKRY
SIFPYSGMFTDKGYSYTAEAITDVDVYYIPTVIFEDMVKANRTQLMHIVQQLSSILKLNENRVQNITIPNAQDRVIQTLN
YLMHDLGEPNGEEIVIPCPLTTIEISKISGTSRETVSSVLKQLKNDRIVTVLGKKITIHNPTYFEESAM

Sequences:

>Translated_229_residues
MNRRNVVKDLKQFELFSHVTDKKLKGLTEFIYWRTYKKGQFLFLEGDSRERIYLMLDGFVKLERVNQSGNLLYEDYVKRY
SIFPYSGMFTDKGYSYTAEAITDVDVYYIPTVIFEDMVKANRTQLMHIVQQLSSILKLNENRVQNITIPNAQDRVIQTLN
YLMHDLGEPNGEEIVIPCPLTTIEISKISGTSRETVSSVLKQLKNDRIVTVLGKKITIHNPTYFEESAM
>Mature_229_residues
MNRRNVVKDLKQFELFSHVTDKKLKGLTEFIYWRTYKKGQFLFLEGDSRERIYLMLDGFVKLERVNQSGNLLYEDYVKRY
SIFPYSGMFTDKGYSYTAEAITDVDVYYIPTVIFEDMVKANRTQLMHIVQQLSSILKLNENRVQNITIPNAQDRVIQTLN
YLMHDLGEPNGEEIVIPCPLTTIEISKISGTSRETVSSVLKQLKNDRIVTVLGKKITIHNPTYFEESAM

Specific function: Has affinity for the xisA upstream region. Binds to a 66 bp region containing three repeats of the consensus recognition sequence 5'-ACATT-3' [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=206, Percent_Identity=25.7281553398058, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR022299
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26657; Mature: 26657

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRNVVKDLKQFELFSHVTDKKLKGLTEFIYWRTYKKGQFLFLEGDSRERIYLMLDGFV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCEEEEEECCCE
KLERVNQSGNLLYEDYVKRYSIFPYSGMFTDKGYSYTAEAITDVDVYYIPTVIFEDMVKA
EEEEECCCCCEEHHHHHHHHCCCCCCCEECCCCCCEEHHHHHCCHHHHHHHHHHHHHHHH
NRTQLMHIVQQLSSILKLNENRVQNITIPNAQDRVIQTLNYLMHDLGEPNGEEIVIPCPL
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
TTIEISKISGTSRETVSSVLKQLKNDRIVTVLGKKITIHNPTYFEESAM
CEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCEEEECCCCCCCCCCC
>Mature Secondary Structure
MNRRNVVKDLKQFELFSHVTDKKLKGLTEFIYWRTYKKGQFLFLEGDSRERIYLMLDGFV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCEEEEEECCCE
KLERVNQSGNLLYEDYVKRYSIFPYSGMFTDKGYSYTAEAITDVDVYYIPTVIFEDMVKA
EEEEECCCCCEEHHHHHHHHCCCCCCCEECCCCCCEEHHHHHCCHHHHHHHHHHHHHHHH
NRTQLMHIVQQLSSILKLNENRVQNITIPNAQDRVIQTLNYLMHDLGEPNGEEIVIPCPL
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
TTIEISKISGTSRETVSSVLKQLKNDRIVTVLGKKITIHNPTYFEESAM
CEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11114927 [H]