Click here to switch to the map view.

The map label for this gene is vanS [H]

Identifier: 152974100

GI number: 152974100

Start: 275554

End: 276933

Strand: Direct

Name: vanS [H]

Synonym: Bcer98_0256

Alternate gene names: 152974100

Gene position: 275554-276933 (Clockwise)

Preceding gene: 152974099

Following gene: 152974101

Centisome position: 6.74

GC content: 33.19

Gene sequence:

>1380_bases
ATGGGAGTGTGGGCATCATCTCCGTTTTTTCAAGGAATAAATAAATATTCTGTAATTGATTATTCATCTGGTATGCGATT
TATTGATACAACCGCTGAAAGTCTTGTAGCGGCTATTGTAAGGGAAGGGGCTTCTGTTAACGTCCAAGAAGTTTTGGAAC
GTCCAGAATATCAAAGAGAGTTAAAAGTTCTAATTGTTGATGAGAACGGGAAAGTTATATATAAGACGAAAGGTGCTCAA
GAAGAACAAATTAATCTTCATAGTACAATTCGTAATATTATGAGTTTCAAAATAAATCGTTCTCAATATGAATATCAAAA
AGATGTAATAGATAATGAAATAGAAAGAGAACGTAAAGAGTTTACAACTTTTTATCCTTTGACAATTGAAGAGAAAAATA
TGTATATGATTGCAAGTGGTATACCACAAGGGAATATTCGAGAAGTACAGAATGATGGCCCGTTTCCGTTTCTAATAGGA
TTTGTTCTTTTTTTATTTTCTTTCTTTTATCTAACAAAAAGAAAAATGAAGCAGATTGAGGCAATGGCCGAAGGGGTAAA
AGAAATTACAAAAGGAAATCTACAACATAGAATTCCAGAGAAGGGACAAGATGAGTTAAGTTTGTTGGCTGTGAATATGA
ATCAGATGGCAGAAGAATTGTTTTCTAATATAGAAAAAGAAAGACGGATAGAAAAGCAAAAAAATGAATTGATTACAAAC
GTATCTCATGATTTACGAACACCCCTTACTTCTATTATGGGGTATTTACGATTGCTTCGTGATTCTAAATACGAAAATAA
AGAACAGCATGATGAATATACAAGAATTGCTTTTACGAAGTCGGAGCAGTTAAAGAATTTAATAGAAGATTTATTCGAGT
ATACGAAATTAACGAATGAAAAGGTCATATTAGAAAAGCAAGAAGTATGTATCAATGAACTTCTAGAACAATTAATAGAA
GAGTTAGTCCCGCAGGCTGAAGAAGAAGGACTGACATTCATAAAACATTTTCCTGAAGAACGTATGTATGCTACGATTGA
TTCGGAGAAGATGGTTCGTGTGTTTGAGAATTTATTAATGAATGCGATTAAGTATAGTAAAGATAATGGAGAGATTAAAG
TATCACTTCAAAGACAAAGACGTGATATCCAAATCATTGTGGCAAATTATAGTGAAGAATTTACAAAAGAAGAATTGGCT
AATTTATTCGAGCGTTTTTATAAGAAAGATCAATCGCGAAGTAGAGTCAAAGAAGGATCTGGGCTTGGTCTCGCGATTGC
AAAAAGCATTGTAGAACTCCAGGGAGGTCAAATTCGAGCTCATTATGAAGATGGAGAGATTCAGTTTATCGTTTCATTAC
CTATCATGGAAGAGAAGTAA

Upstream 100 bases:

>100_bases
GATTTTAAAAAAAATAATATATAAAGGAATATGTGGGGTACAAAAGAGTTTGCGTGTACAACTGATAACGACGTTTGCTC
TCTGTAGCTTGCTGGGAATC

Downstream 100 bases:

>100_bases
ATGAAGGTGTTTTATCCCGCTGTTCTCGAGCAGTAAGACTCCCACCTCAAGGTTCAAAGAGTAGCAAGAAGATAGGTGGA
AGTAGGGCTGCCCGTAACAG

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MGVWASSPFFQGINKYSVIDYSSGMRFIDTTAESLVAAIVREGASVNVQEVLERPEYQRELKVLIVDENGKVIYKTKGAQ
EEQINLHSTIRNIMSFKINRSQYEYQKDVIDNEIERERKEFTTFYPLTIEEKNMYMIASGIPQGNIREVQNDGPFPFLIG
FVLFLFSFFYLTKRKMKQIEAMAEGVKEITKGNLQHRIPEKGQDELSLLAVNMNQMAEELFSNIEKERRIEKQKNELITN
VSHDLRTPLTSIMGYLRLLRDSKYENKEQHDEYTRIAFTKSEQLKNLIEDLFEYTKLTNEKVILEKQEVCINELLEQLIE
ELVPQAEEEGLTFIKHFPEERMYATIDSEKMVRVFENLLMNAIKYSKDNGEIKVSLQRQRRDIQIIVANYSEEFTKEELA
NLFERFYKKDQSRSRVKEGSGLGLAIAKSIVELQGGQIRAHYEDGEIQFIVSLPIMEEK

Sequences:

>Translated_459_residues
MGVWASSPFFQGINKYSVIDYSSGMRFIDTTAESLVAAIVREGASVNVQEVLERPEYQRELKVLIVDENGKVIYKTKGAQ
EEQINLHSTIRNIMSFKINRSQYEYQKDVIDNEIERERKEFTTFYPLTIEEKNMYMIASGIPQGNIREVQNDGPFPFLIG
FVLFLFSFFYLTKRKMKQIEAMAEGVKEITKGNLQHRIPEKGQDELSLLAVNMNQMAEELFSNIEKERRIEKQKNELITN
VSHDLRTPLTSIMGYLRLLRDSKYENKEQHDEYTRIAFTKSEQLKNLIEDLFEYTKLTNEKVILEKQEVCINELLEQLIE
ELVPQAEEEGLTFIKHFPEERMYATIDSEKMVRVFENLLMNAIKYSKDNGEIKVSLQRQRRDIQIIVANYSEEFTKEELA
NLFERFYKKDQSRSRVKEGSGLGLAIAKSIVELQGGQIRAHYEDGEIQFIVSLPIMEEK
>Mature_458_residues
GVWASSPFFQGINKYSVIDYSSGMRFIDTTAESLVAAIVREGASVNVQEVLERPEYQRELKVLIVDENGKVIYKTKGAQE
EQINLHSTIRNIMSFKINRSQYEYQKDVIDNEIERERKEFTTFYPLTIEEKNMYMIASGIPQGNIREVQNDGPFPFLIGF
VLFLFSFFYLTKRKMKQIEAMAEGVKEITKGNLQHRIPEKGQDELSLLAVNMNQMAEELFSNIEKERRIEKQKNELITNV
SHDLRTPLTSIMGYLRLLRDSKYENKEQHDEYTRIAFTKSEQLKNLIEDLFEYTKLTNEKVILEKQEVCINELLEQLIEE
LVPQAEEEGLTFIKHFPEERMYATIDSEKMVRVFENLLMNAIKYSKDNGEIKVSLQRQRRDIQIIVANYSEEFTKEELAN
LFERFYKKDQSRSRVKEGSGLGLAIAKSIVELQGGQIRAHYEDGEIQFIVSLPIMEEK

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=283, Percent_Identity=27.208480565371, Blast_Score=121, Evalue=9e-29,
Organism=Escherichia coli, GI1786783, Length=304, Percent_Identity=26.9736842105263, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790346, Length=283, Percent_Identity=28.2685512367491, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788713, Length=242, Percent_Identity=27.6859504132231, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789149, Length=325, Percent_Identity=26.1538461538462, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI145693157, Length=245, Percent_Identity=27.3469387755102, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI48994928, Length=222, Percent_Identity=27.4774774774775, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786912, Length=283, Percent_Identity=27.208480565371, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787894, Length=322, Percent_Identity=27.0186335403727, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87082128, Length=304, Percent_Identity=26.6447368421053, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786600, Length=209, Percent_Identity=25.3588516746411, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788279, Length=254, Percent_Identity=27.1653543307087, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790436, Length=227, Percent_Identity=26.431718061674, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788549, Length=247, Percent_Identity=24.6963562753036, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790861, Length=387, Percent_Identity=21.9638242894057, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789808, Length=297, Percent_Identity=26.2626262626263, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI87081816, Length=235, Percent_Identity=24.2553191489362, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53611; Mature: 53480

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVWASSPFFQGINKYSVIDYSSGMRFIDTTAESLVAAIVREGASVNVQEVLERPEYQRE
CCCCCCCHHHHCCCCCEEEEECCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCC
LKVLIVDENGKVIYKTKGAQEEQINLHSTIRNIMSFKINRSQYEYQKDVIDNEIERERKE
EEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
FTTFYPLTIEEKNMYMIASGIPQGNIREVQNDGPFPFLIGFVLFLFSFFYLTKRKMKQIE
HHEEECEEEECCCEEEEECCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AMAEGVKEITKGNLQHRIPEKGQDELSLLAVNMNQMAEELFSNIEKERRIEKQKNELITN
HHHHHHHHHHCCCHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSHDLRTPLTSIMGYLRLLRDSKYENKEQHDEYTRIAFTKSEQLKNLIEDLFEYTKLTNE
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEECHHHHHHHHHHHHHHHHHCCCC
KVILEKQEVCINELLEQLIEELVPQAEEEGLTFIKHFPEERMYATIDSEKMVRVFENLLM
CEEHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHCCCHHEEEECCHHHHHHHHHHHHH
NAIKYSKDNGEIKVSLQRQRRDIQIIVANYSEEFTKEELANLFERFYKKDQSRSRVKEGS
HHHHHCCCCCCEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCC
GLGLAIAKSIVELQGGQIRAHYEDGEIQFIVSLPIMEEK
CCHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCC
>Mature Secondary Structure 
GVWASSPFFQGINKYSVIDYSSGMRFIDTTAESLVAAIVREGASVNVQEVLERPEYQRE
CCCCCCHHHHCCCCCEEEEECCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCC
LKVLIVDENGKVIYKTKGAQEEQINLHSTIRNIMSFKINRSQYEYQKDVIDNEIERERKE
EEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
FTTFYPLTIEEKNMYMIASGIPQGNIREVQNDGPFPFLIGFVLFLFSFFYLTKRKMKQIE
HHEEECEEEECCCEEEEECCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AMAEGVKEITKGNLQHRIPEKGQDELSLLAVNMNQMAEELFSNIEKERRIEKQKNELITN
HHHHHHHHHHCCCHHHCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSHDLRTPLTSIMGYLRLLRDSKYENKEQHDEYTRIAFTKSEQLKNLIEDLFEYTKLTNE
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEECHHHHHHHHHHHHHHHHHCCCC
KVILEKQEVCINELLEQLIEELVPQAEEEGLTFIKHFPEERMYATIDSEKMVRVFENLLM
CEEHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHCCCHHEEEECCHHHHHHHHHHHHH
NAIKYSKDNGEIKVSLQRQRRDIQIIVANYSEEFTKEELANLFERFYKKDQSRSRVKEGS
HHHHHCCCCCCEEEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCC
GLGLAIAKSIVELQGGQIRAHYEDGEIQFIVSLPIMEEK
CCHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]