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The map label for this gene is cysK [H]

Identifier: 152973916

GI number: 152973916

Start: 73830

End: 74753

Strand: Direct

Name: cysK [H]

Synonym: Bcer98_0063

Alternate gene names: 152973916

Gene position: 73830-74753 (Clockwise)

Preceding gene: 152973915

Following gene: 152973917

Centisome position: 1.81

GC content: 40.37

Gene sequence:

>924_bases
ATGCGAGTGGCACAATCAGTTTCAGAATTAATCGGGAAAACGCCGATCGTTAAGTTGAACCGCATCGTAGAGCAAGACAG
TGCAGATATATATTTAAAATTAGAATTTATGAATCCTGGAAGTAGCGTGAAAGATCGTATTGCATTATCTATGATCGAAG
CTGCTGAGAAAAAAGGTTTATTAAAAGAAGGCGATACAATCATTGAGCCAACAAGTGGTAACACAGGGATTGGCTTAGCG
ATGGTGGCAGCTGCAAAAGGATATAAAGCAATTTTAGTAATGCCGGAGACAATGAGTATGGAACGCCGTAATTTACTTCG
AGCTTACGGGGCAGAATTGGTACTAACTCCAGGTTCAGAAGGAATGGGTGGCGCAATTCGTAAGGCGACGGAATTAGCAA
AAGAGAACGGTTATTTCATGCCACAACAATTCCAAAATCCAGCAAACCCAGAAATTCATCGTTTAACAACAGGTCCAGAA
ATTGTTGAGCAGATGGGTGACCAATTAGATGCATTTATCGCAGGAATTGGTACTGGCGGCACAATTACTGGTGCTGGGGA
AGTATTAAAAGAAACGTATAAAGACATTAAAATTTATGCAGTCGAGCCAGCAGACTCTCCTGTACTATCGGGTGGAAAAC
CAGGTCCACATAAAATCCAAGGAATTGGAGCTGGATTTGTTCCGGAAACATTGGACGTAGAAATATATGATGATATCATT
CAAGTGAAGACAGAACAAGCATTTGAATATGCAAGAAAAGTAGCTCGTGAAGAAGGGATTTTAGTCGGTATTTCTTCAGG
TGCTGTTATTTATGCTGCAACAGAAGTTGCGAAAAAACTTGGTAAAGGGAAAAAGGTTCTTGTGGTCATTCCAAGTAACG
GTGAACGCTATTTAAGTACCCCTCTTTACCAATTTGAATCATAA

Upstream 100 bases:

>100_bases
ATAACATGTTGTTTAAATAGATTGACAAATGAAGAATTTTCTGACAAGATATTAATGTGGAGAAAACCAATAAAAATACT
CGGTGTTAGGAGTGACAGGA

Downstream 100 bases:

>100_bases
TTTGTGTTGCTAGAAAAAAGCATTCCTGAAAAGGGATGCTTTTTTCTTTTGGAAATAGGAAAAGAGTGAATGACTAGAAA
ACTTCATGTAAAATAAGTAG

Product: cysteine synthase A

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; Superoxide-inducible protein 11; SOI11 [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MRVAQSVSELIGKTPIVKLNRIVEQDSADIYLKLEFMNPGSSVKDRIALSMIEAAEKKGLLKEGDTIIEPTSGNTGIGLA
MVAAAKGYKAILVMPETMSMERRNLLRAYGAELVLTPGSEGMGGAIRKATELAKENGYFMPQQFQNPANPEIHRLTTGPE
IVEQMGDQLDAFIAGIGTGGTITGAGEVLKETYKDIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPETLDVEIYDDII
QVKTEQAFEYARKVAREEGILVGISSGAVIYAATEVAKKLGKGKKVLVVIPSNGERYLSTPLYQFES

Sequences:

>Translated_307_residues
MRVAQSVSELIGKTPIVKLNRIVEQDSADIYLKLEFMNPGSSVKDRIALSMIEAAEKKGLLKEGDTIIEPTSGNTGIGLA
MVAAAKGYKAILVMPETMSMERRNLLRAYGAELVLTPGSEGMGGAIRKATELAKENGYFMPQQFQNPANPEIHRLTTGPE
IVEQMGDQLDAFIAGIGTGGTITGAGEVLKETYKDIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPETLDVEIYDDII
QVKTEQAFEYARKVAREEGILVGISSGAVIYAATEVAKKLGKGKKVLVVIPSNGERYLSTPLYQFES
>Mature_307_residues
MRVAQSVSELIGKTPIVKLNRIVEQDSADIYLKLEFMNPGSSVKDRIALSMIEAAEKKGLLKEGDTIIEPTSGNTGIGLA
MVAAAKGYKAILVMPETMSMERRNLLRAYGAELVLTPGSEGMGGAIRKATELAKENGYFMPQQFQNPANPEIHRLTTGPE
IVEQMGDQLDAFIAGIGTGGTITGAGEVLKETYKDIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPETLDVEIYDDII
QVKTEQAFEYARKVAREEGILVGISSGAVIYAATEVAKKLGKGKKVLVVIPSNGERYLSTPLYQFES

Specific function: Catalyzes the conversion of O-acetylserine to cysteine. Also acts as a sensor of cysteine availability in the signal transduction pathway modulating CymR activity. When cysteine is present, the pool of O-acetylserine (OAS) is low, which leads to the forma

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=303, Percent_Identity=41.2541254125413, Blast_Score=228, Evalue=5e-60,
Organism=Homo sapiens, GI295821200, Length=303, Percent_Identity=41.2541254125413, Blast_Score=228, Evalue=5e-60,
Organism=Homo sapiens, GI4557415, Length=303, Percent_Identity=41.2541254125413, Blast_Score=228, Evalue=5e-60,
Organism=Escherichia coli, GI1788754, Length=313, Percent_Identity=53.3546325878594, Blast_Score=285, Evalue=2e-78,
Organism=Escherichia coli, GI2367138, Length=298, Percent_Identity=41.6107382550336, Blast_Score=231, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI17535051, Length=299, Percent_Identity=49.8327759197324, Blast_Score=285, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI115535073, Length=305, Percent_Identity=50.4918032786885, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17562970, Length=306, Percent_Identity=48.0392156862745, Blast_Score=270, Evalue=6e-73,
Organism=Caenorhabditis elegans, GI32566674, Length=300, Percent_Identity=48.3333333333333, Blast_Score=265, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI17561720, Length=300, Percent_Identity=46, Blast_Score=233, Evalue=6e-62,
Organism=Caenorhabditis elegans, GI32566672, Length=213, Percent_Identity=48.3568075117371, Blast_Score=194, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI17534315, Length=317, Percent_Identity=39.7476340694006, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI25147552, Length=315, Percent_Identity=37.4603174603175, Blast_Score=165, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17561716, Length=117, Percent_Identity=45.2991452991453, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71996324, Length=301, Percent_Identity=26.9102990033223, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6321594, Length=315, Percent_Identity=38.4126984126984, Blast_Score=192, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6321449, Length=330, Percent_Identity=35.1515151515151, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24643623, Length=309, Percent_Identity=40.1294498381877, Blast_Score=205, Evalue=3e-53,
Organism=Drosophila melanogaster, GI20129101, Length=309, Percent_Identity=40.1294498381877, Blast_Score=205, Evalue=3e-53,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32918; Mature: 32918

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVAQSVSELIGKTPIVKLNRIVEQDSADIYLKLEFMNPGSSVKDRIALSMIEAAEKKGL
CCHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCC
LKEGDTIIEPTSGNTGIGLAMVAAAKGYKAILVMPETMSMERRNLLRAYGAELVLTPGSE
CCCCCEEEECCCCCCCCHHHEEECCCCCEEEEEECCHHHHHHHHHHHHCCCEEEECCCCC
GMGGAIRKATELAKENGYFMPQQFQNPANPEIHRLTTGPEIVEQMGDQLDAFIAGIGTGG
CCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCC
TITGAGEVLKETYKDIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPETLDVEIYDDII
EECCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHH
QVKTEQAFEYARKVAREEGILVGISSGAVIYAATEVAKKLGKGKKVLVVIPSNGERYLST
HHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEHHHHHHHHCCCCEEEEEECCCCCCEECC
PLYQFES
CCHHCCC
>Mature Secondary Structure
MRVAQSVSELIGKTPIVKLNRIVEQDSADIYLKLEFMNPGSSVKDRIALSMIEAAEKKGL
CCHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCC
LKEGDTIIEPTSGNTGIGLAMVAAAKGYKAILVMPETMSMERRNLLRAYGAELVLTPGSE
CCCCCEEEECCCCCCCCHHHEEECCCCCEEEEEECCHHHHHHHHHHHHCCCEEEECCCCC
GMGGAIRKATELAKENGYFMPQQFQNPANPEIHRLTTGPEIVEQMGDQLDAFIAGIGTGG
CCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCC
TITGAGEVLKETYKDIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPETLDVEIYDDII
EECCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHH
QVKTEQAFEYARKVAREEGILVGISSGAVIYAATEVAKKLGKGKKVLVVIPSNGERYLST
HHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEHHHHHHHHCCCCEEEEEECCCCCCEECC
PLYQFES
CCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 8755892; 9298659 [H]