Click here to switch to the map view.

The map label for this gene is pth [H]

Identifier: 152973899

GI number: 152973899

Start: 53942

End: 54502

Strand: Direct

Name: pth [H]

Synonym: Bcer98_0046

Alternate gene names: 152973899

Gene position: 53942-54502 (Clockwise)

Preceding gene: 152973898

Following gene: 152973900

Centisome position: 1.32

GC content: 36.54

Gene sequence:

>561_bases
ATGAAATTAATAGTAGGACTTGGGAACCCTGGTAGAGAATATGAATTAACAAGGCATAATATTGGATTTATGGCGATTGA
TGAACTTGCGAAGCGATGGAACATTTCTTTAAATGAACAAAAGTTTAAGGGGATGTTTGGAGCAGGTTTTGTGAATGGCG
AAAAAGTGATTTTATTAAAGCCGCTTACATATATGAATTTGTCTGGAGAAAGCATCCGTCCGCTTATGGATTATTATAAG
ATTGATTTAGAAGACTTTATTATTATGTATGATGATTTAGATCTTCCTGTAGGGAAATTACGCCTTCGTATGAAAGGAAG
TGCGGGTGGTCATAACGGAGTAAAGTCGACAATCGCTCATTTAGGAACACAAGAGTTCCAACGTATTCGTATGGGAATTG
ATCGCCCTAAAAATGGAATGAAGGTAGTAGACTATGTGCTAGGACGTTTTACAGCGGAAGAAATGGTAGATGTAAATCAT
GCTATTGAGAAAGCTGCTAATGCATGTGAGGAGTGGCTTAACAAATCGTTTCTTCAAGTAATGAACGATTTTAATAACTA
A

Upstream 100 bases:

>100_bases
AGAATCTGTAAGTGTATTATTCAATTAATTGGATAGAATGAGACGTAACCAAATTTGGTTACGTCTTTTCGTATCGTAGA
AAGAAAGTAGTGGTGGAAGA

Downstream 100 bases:

>100_bases
TTTTATAAATTCGAATATTTTATCATGTTTTGATCCATACTAGTAGTAATTGTACCTTTAGGAGGATAGTATGGACGGAC
ATTATTATTGTGGGCATTGT

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MKLIVGLGNPGREYELTRHNIGFMAIDELAKRWNISLNEQKFKGMFGAGFVNGEKVILLKPLTYMNLSGESIRPLMDYYK
IDLEDFIIMYDDLDLPVGKLRLRMKGSAGGHNGVKSTIAHLGTQEFQRIRMGIDRPKNGMKVVDYVLGRFTAEEMVDVNH
AIEKAANACEEWLNKSFLQVMNDFNN

Sequences:

>Translated_186_residues
MKLIVGLGNPGREYELTRHNIGFMAIDELAKRWNISLNEQKFKGMFGAGFVNGEKVILLKPLTYMNLSGESIRPLMDYYK
IDLEDFIIMYDDLDLPVGKLRLRMKGSAGGHNGVKSTIAHLGTQEFQRIRMGIDRPKNGMKVVDYVLGRFTAEEMVDVNH
AIEKAANACEEWLNKSFLQVMNDFNN
>Mature_186_residues
MKLIVGLGNPGREYELTRHNIGFMAIDELAKRWNISLNEQKFKGMFGAGFVNGEKVILLKPLTYMNLSGESIRPLMDYYK
IDLEDFIIMYDDLDLPVGKLRLRMKGSAGGHNGVKSTIAHLGTQEFQRIRMGIDRPKNGMKVVDYVLGRFTAEEMVDVNH
AIEKAANACEEWLNKSFLQVMNDFNN

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis [H]

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family [H]

Homologues:

Organism=Homo sapiens, GI50897284, Length=155, Percent_Identity=32.9032258064516, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI1787455, Length=184, Percent_Identity=40.2173913043478, Blast_Score=125, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321983, Length=176, Percent_Identity=28.4090909090909, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001328
- InterPro:   IPR018171 [H]

Pfam domain/function: PF01195 Pept_tRNA_hydro [H]

EC number: =3.1.1.29 [H]

Molecular weight: Translated: 21163; Mature: 21163

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1 ; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIVGLGNPGREYELTRHNIGFMAIDELAKRWNISLNEQKFKGMFGAGFVNGEKVILLK
CEEEEECCCCCCCEEEHHCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCEECCCEEEEEC
PLTYMNLSGESIRPLMDYYKIDLEDFIIMYDDLDLPVGKLRLRMKGSAGGHNGVKSTIAH
CCEEECCCCCCHHHHHHHHHCCHHHEEEEEECCCCCHHHEEEEECCCCCCCCHHHHHHHH
LGTQEFQRIRMGIDRPKNGMKVVDYVLGRFTAEEMVDVNHAIEKAANACEEWLNKSFLQV
HCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNDFNN
HHHCCC
>Mature Secondary Structure
MKLIVGLGNPGREYELTRHNIGFMAIDELAKRWNISLNEQKFKGMFGAGFVNGEKVILLK
CEEEEECCCCCCCEEEHHCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCEECCCEEEEEC
PLTYMNLSGESIRPLMDYYKIDLEDFIIMYDDLDLPVGKLRLRMKGSAGGHNGVKSTIAH
CCEEECCCCCCHHHHHHHHHCCHHHEEEEEECCCCCHHHEEEEECCCCCCCCHHHHHHHH
LGTQEFQRIRMGIDRPKNGMKVVDYVLGRFTAEEMVDVNHAIEKAANACEEWLNKSFLQV
HCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNDFNN
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]