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The map label for this gene is dnaN [H]

Identifier: 152973856

GI number: 152973856

Start: 2043

End: 3188

Strand: Direct

Name: dnaN [H]

Synonym: Bcer98_0002

Alternate gene names: 152973856

Gene position: 2043-3188 (Clockwise)

Preceding gene: 152973855

Following gene: 152973857

Centisome position: 0.05

GC content: 32.37

Gene sequence:

>1146_bases
ATGCGTTTTTCAATTCAAAAAGACTATCTTGTAAGAAGTGTACAAGATGTAATGAAAGCTGTTTCCTCTCGCACCACAAT
TCCGATCCTTACTGGAATTAAAGTTGTAGCTACAGAAGAAGGAGTTACATTAACAGGAAGTGATTCAGATATTTCAATCG
AATCATTTATTCCTGTTGAAGAAGATGGAAAAGAAATCGTAGAAGTTAGTCAATCAGGTAGTATTGTTTTACAAGCAAAA
TATTTTAGTGAAATCGTAAAAAAATTACCAAAAGACATTGTCGAAATTTCCGTCGAAAATCATTTTATGACAAAAATAAA
ATCTGGAAAATCAGAGTTTAATTTAAATGGATTAGATTCTGCGGAATATCCATTATTACCACAAATCGAAGAACATCATG
TATTTAAAATACCAACAGATTTACTAAAACATATGATTCGTCAAACTGTATTTGCAGTATCAAGTTCTGAAACTCGCCCA
ATCTTGACAGGTGTAAACTGGAAGGTATATAACAGTGAGTTAACTTGTATTGCAACAGATAGTCACAGATTGGCACTTCG
CACAGCGAAAATTGAAGGACATAACATTTCTGATGAATTTCAAGCAAATGTTGTCATTCCAGGTAAAAGTTTAAATGAGT
TAAGTAAAATTTTAGATGAGTCTGAAGAAATGGTGGATATTGTTATTACAGAGTATCAAGTATTATTCCGAACAAAACAT
TTATTATTCTTCTCTCGATTATTGGAAGGGAATTACCCTGATACAACTCGCTTAATTCCTGCTGAAAGTAAGACGGATAT
CTTTGTCAATACGAAAGAGTTTTTGCAAGCTATTGATCGCGCTTCCTTGTTGGCAAGAGATGGGCGTAACAACGTTGTAA
AACTATCAACATTTGAACAGAAAATGCTAGAAATTTCTTCGAATTCACCGGAAATTGGGAAAGTAGTAGAAGAAGTTCAA
TGTGAAAAAATAGATGGTGAAGAATTAAAAATATCATTTAGTGCAAAATATATGATGGATGCATTAAAAGCTTTAGATAG
TACTGAAATTAAAATTAGCTTTACTGGTGCGATGAGACCTTTCTTAATTCGTACAGTAAATGATAACTCCATTATTCAAC
TAATTTTACCGGTTCGTACTTACTAA

Upstream 100 bases:

>100_bases
GGGGTTATCCACATATCCACAAGCCCTATTACTATTACTACTACTTTTTATCTTTATTAAATAAATAAATAAAATCTTAT
ACTTACCGGAGGTTTTCACT

Downstream 100 bases:

>100_bases
AAAGAATGAGGGTTGCTAGTTTTTATGTACTAGCGACCCTTGTTTGATTTTGGGTATTACTTTCCTAATGCTAGTTTATT
TAGTACAATGAAAGAATGAA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MRFSIQKDYLVRSVQDVMKAVSSRTTIPILTGIKVVATEEGVTLTGSDSDISIESFIPVEEDGKEIVEVSQSGSIVLQAK
YFSEIVKKLPKDIVEISVENHFMTKIKSGKSEFNLNGLDSAEYPLLPQIEEHHVFKIPTDLLKHMIRQTVFAVSSSETRP
ILTGVNWKVYNSELTCIATDSHRLALRTAKIEGHNISDEFQANVVIPGKSLNELSKILDESEEMVDIVITEYQVLFRTKH
LLFFSRLLEGNYPDTTRLIPAESKTDIFVNTKEFLQAIDRASLLARDGRNNVVKLSTFEQKMLEISSNSPEIGKVVEEVQ
CEKIDGEELKISFSAKYMMDALKALDSTEIKISFTGAMRPFLIRTVNDNSIIQLILPVRTY

Sequences:

>Translated_381_residues
MRFSIQKDYLVRSVQDVMKAVSSRTTIPILTGIKVVATEEGVTLTGSDSDISIESFIPVEEDGKEIVEVSQSGSIVLQAK
YFSEIVKKLPKDIVEISVENHFMTKIKSGKSEFNLNGLDSAEYPLLPQIEEHHVFKIPTDLLKHMIRQTVFAVSSSETRP
ILTGVNWKVYNSELTCIATDSHRLALRTAKIEGHNISDEFQANVVIPGKSLNELSKILDESEEMVDIVITEYQVLFRTKH
LLFFSRLLEGNYPDTTRLIPAESKTDIFVNTKEFLQAIDRASLLARDGRNNVVKLSTFEQKMLEISSNSPEIGKVVEEVQ
CEKIDGEELKISFSAKYMMDALKALDSTEIKISFTGAMRPFLIRTVNDNSIIQLILPVRTY
>Mature_381_residues
MRFSIQKDYLVRSVQDVMKAVSSRTTIPILTGIKVVATEEGVTLTGSDSDISIESFIPVEEDGKEIVEVSQSGSIVLQAK
YFSEIVKKLPKDIVEISVENHFMTKIKSGKSEFNLNGLDSAEYPLLPQIEEHHVFKIPTDLLKHMIRQTVFAVSSSETRP
ILTGVNWKVYNSELTCIATDSHRLALRTAKIEGHNISDEFQANVVIPGKSLNELSKILDESEEMVDIVITEYQVLFRTKH
LLFFSRLLEGNYPDTTRLIPAESKTDIFVNTKEFLQAIDRASLLARDGRNNVVKLSTFEQKMLEISSNSPEIGKVVEEVQ
CEKIDGEELKISFSAKYMMDALKALDSTEIKISFTGAMRPFLIRTVNDNSIIQLILPVRTY

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=380, Percent_Identity=25.5263157894737, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42913; Mature: 42913

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSIQKDYLVRSVQDVMKAVSSRTTIPILTGIKVVATEEGVTLTGSDSDISIESFIPVE
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECEEEEEECCCEEEECCCCCEEHEEECCCC
EDGKEIVEVSQSGSIVLQAKYFSEIVKKLPKDIVEISVENHFMTKIKSGKSEFNLNGLDS
CCCHHHHHCCCCCCEEEEEHHHHHHHHHCCHHHHEEECCHHHHHHHHCCCCCCCCCCCCC
AEYPLLPQIEEHHVFKIPTDLLKHMIRQTVFAVSSSETRPILTGVNWKVYNSELTCIATD
CCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCEEECCEEEEECCEEEEEEEC
SHRLALRTAKIEGHNISDEFQANVVIPGKSLNELSKILDESEEMVDIVITEYQVLFRTKH
CCEEEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LLFFSRLLEGNYPDTTRLIPAESKTDIFVNTKEFLQAIDRASLLARDGRNNVVKLSTFEQ
HHHHHHHHCCCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEHHHH
KMLEISSNSPEIGKVVEEVQCEKIDGEELKISFSAKYMMDALKALDSTEIKISFTGAMRP
HHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEEEECCCCC
FLIRTVNDNSIIQLILPVRTY
EEEEEECCCCEEEEEEECCCC
>Mature Secondary Structure
MRFSIQKDYLVRSVQDVMKAVSSRTTIPILTGIKVVATEEGVTLTGSDSDISIESFIPVE
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECEEEEEECCCEEEECCCCCEEHEEECCCC
EDGKEIVEVSQSGSIVLQAKYFSEIVKKLPKDIVEISVENHFMTKIKSGKSEFNLNGLDS
CCCHHHHHCCCCCCEEEEEHHHHHHHHHCCHHHHEEECCHHHHHHHHCCCCCCCCCCCCC
AEYPLLPQIEEHHVFKIPTDLLKHMIRQTVFAVSSSETRPILTGVNWKVYNSELTCIATD
CCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCEEECCEEEEECCEEEEEEEC
SHRLALRTAKIEGHNISDEFQANVVIPGKSLNELSKILDESEEMVDIVITEYQVLFRTKH
CCEEEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LLFFSRLLEGNYPDTTRLIPAESKTDIFVNTKEFLQAIDRASLLARDGRNNVVKLSTFEQ
HHHHHHHHCCCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEHHHH
KMLEISSNSPEIGKVVEEVQCEKIDGEELKISFSAKYMMDALKALDSTEIKISFTGAMRP
HHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEEEECCCCC
FLIRTVNDNSIIQLILPVRTY
EEEEEECCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377; 2846289 [H]