| Definition | Klebsiella pneumoniae subsp. pneumoniae MGH 78578 chromosome, complete genome. |
|---|---|
| Accession | NC_009648 |
| Length | 5,315,120 |
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The map label for this gene is ilvI [H]
Identifier: 152971607
GI number: 152971607
Start: 3381456
End: 3383165
Strand: Direct
Name: ilvI [H]
Synonym: KPN_03085
Alternate gene names: 152971607
Gene position: 3381456-3383165 (Clockwise)
Preceding gene: 152971606
Following gene: 152971609
Centisome position: 63.62
GC content: 64.27
Gene sequence:
>1710_bases ATGGCCTCTATCACATTATCGGCTGCTGAACTTTTACTGCATCGGCTACAGGCGCTGGACGTGGCGTATATTTTTATCAA CTCGGGCACCGATTATCCTCCAGTCATTGAAGCCTGGGCAAAGGCCCGGGCGACCGGGCAAAAGGTACCGGAGTTAGTGA TCTGCCCGCATGAAAACGCCGCCATCGGCATGGCCCATGGCTATTACCTCGGCACCGGCAAGGTGCAGGCGGTGATGGTT CACACCAACGTCGGGCTAGCCAACGCCGCCTGCGGCGTCATCAATCTGGCGAACAGCAATATTCCGGTGCTGATTTTTGG CGGCCGCACGCCGATAAGCGAACATTCCCACTTCGGCTGCCGTAATACGCCGATTGGCTACGGCCAGGAGATGCGCGACC AGGCGGCGCTGATCCGCGAATCCGTGAAGTGGGATTTTGAACTGCGGCTGGCCGATCAGATCGGCGAGCATGTTGACCGG GCCTGGGCGATCGCCAGCTCCCTGCCGAAAGGGCCGGTCTACCTGAGCTTACCGCGTGAGCCACTGTGCGAAACCTTTGC CGTCGATGAGGCCGCGCTGCAGGCTGGCCCTTCGCAGCAGCCGGTCCGCTATGCGCCAGTCCGGGAAGATATCGCCCGCG CAGCCGAGGCAATCGCCTGCGCCAGACACCCGGTCATCTTCGCCCAGCGCGGCGCCCGAACGGCGGAAGGCTTTGCGCGC CTCGATAGTCTGGTGCGCGAATGGGCGATCCCTCTGGTGGAATACTGGGGCACCGAGGTTACCCTGAGCGCCGATAATCC CCTGCTCGCCGGGGCTGATCCCAGCGTCTGGCTCGCTGACGCCGATGTGATTCTGGTGGTCGACTCGCAGGCACCATGGA TGATCGCCGAGGGCGACTGTCGCCAGGACTGCACCGTGATCCAGATGGGGCCCGATCCGCTCTTCTCCCGCTACCCGGTG CGCGGCTATCGCGCGGACATCAACCTGGCGGGCGAGACCGATGAAGTCTTCAGCCTGCTGGAAGAGGCGCTGCGCCCGCT GCAGGCCGCCCGCCAGCAGCATGTGGCGGAGCGGGCGGCATATACGCAGAGCCGCATCCAGCAACAGAAAAACCAGCGCG ACGCGCTTCTCCATGCCAGCCAGAGCGGGGCCATTACCAAACCCTGGCTCAGCTACTGCCTCGGCCATCTGGCTAATCAA CACCGCGGCCGGATCGTTTCTGAACTGACCACGCTGCCGCAGTTTGCCGGACTGACCCATGCCGAGAGCTATTACCAGGA AGCGCTGGCCGGCGGCCTGGGCGAAGCCCTGCCCATCGCGCTGGGGCTGCAGCTTGCGCGCCGGGAAGAGCTGATTATTG CCGCCGTCGGCGACGGGTCGTACCTTTTCGCCAACCCTGCGGTCTGCCACCATATTGCGGAGGTAATGAAGCTTCCTGTC CTCGTCGTGGTCGGAAATAACGGCGGATGGGGCGCCGTCGCGGGCGGCACCAAAGCGCTCTACCCGGACGGCTACGCGGC CCGCGCGGAAACCATTCCGGCCACCGCCTTTACCACCTCGCCGGATTTCGCCGCGATCGCCGCCTCCAGCCGCGCGGCGG CCCTGTCGGTTTCCCGGGCCGAAGATCTCCCCGGCGTGCTGGAAGAAGCGGTATCCCTCATACGCACCCGCCGACAAAGC GTGCTGGTTGACGTGCAGTTAGCCCGCTAA
Upstream 100 bases:
>100_bases CGATGGCGCTGAGTATTATTGCCTCCATCATTACGATGGGCGGATTTATTTTCTTAACCTTTTAATTACCGCGATTACAC CGCGTGAATACGGAGAGAAT
Downstream 100 bases:
>100_bases CGGTTTATCCTCCGGGCGCTTGTCGTCCGGAGGGGGTTGTCACCCCTTCTGGTCACCTTTATCCACATGGCCCAGCGGCT TCTCCGGGAAGCAGACATCG
Product: acetolactate synthase catalytic subunit
Products: NA
Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]
Number of amino acids: Translated: 569; Mature: 568
Protein sequence:
>569_residues MASITLSAAELLLHRLQALDVAYIFINSGTDYPPVIEAWAKARATGQKVPELVICPHENAAIGMAHGYYLGTGKVQAVMV HTNVGLANAACGVINLANSNIPVLIFGGRTPISEHSHFGCRNTPIGYGQEMRDQAALIRESVKWDFELRLADQIGEHVDR AWAIASSLPKGPVYLSLPREPLCETFAVDEAALQAGPSQQPVRYAPVREDIARAAEAIACARHPVIFAQRGARTAEGFAR LDSLVREWAIPLVEYWGTEVTLSADNPLLAGADPSVWLADADVILVVDSQAPWMIAEGDCRQDCTVIQMGPDPLFSRYPV RGYRADINLAGETDEVFSLLEEALRPLQAARQQHVAERAAYTQSRIQQQKNQRDALLHASQSGAITKPWLSYCLGHLANQ HRGRIVSELTTLPQFAGLTHAESYYQEALAGGLGEALPIALGLQLARREELIIAAVGDGSYLFANPAVCHHIAEVMKLPV LVVVGNNGGWGAVAGGTKALYPDGYAARAETIPATAFTTSPDFAAIAASSRAAALSVSRAEDLPGVLEEAVSLIRTRRQS VLVDVQLAR
Sequences:
>Translated_569_residues MASITLSAAELLLHRLQALDVAYIFINSGTDYPPVIEAWAKARATGQKVPELVICPHENAAIGMAHGYYLGTGKVQAVMV HTNVGLANAACGVINLANSNIPVLIFGGRTPISEHSHFGCRNTPIGYGQEMRDQAALIRESVKWDFELRLADQIGEHVDR AWAIASSLPKGPVYLSLPREPLCETFAVDEAALQAGPSQQPVRYAPVREDIARAAEAIACARHPVIFAQRGARTAEGFAR LDSLVREWAIPLVEYWGTEVTLSADNPLLAGADPSVWLADADVILVVDSQAPWMIAEGDCRQDCTVIQMGPDPLFSRYPV RGYRADINLAGETDEVFSLLEEALRPLQAARQQHVAERAAYTQSRIQQQKNQRDALLHASQSGAITKPWLSYCLGHLANQ HRGRIVSELTTLPQFAGLTHAESYYQEALAGGLGEALPIALGLQLARREELIIAAVGDGSYLFANPAVCHHIAEVMKLPV LVVVGNNGGWGAVAGGTKALYPDGYAARAETIPATAFTTSPDFAAIAASSRAAALSVSRAEDLPGVLEEAVSLIRTRRQS VLVDVQLAR >Mature_568_residues ASITLSAAELLLHRLQALDVAYIFINSGTDYPPVIEAWAKARATGQKVPELVICPHENAAIGMAHGYYLGTGKVQAVMVH TNVGLANAACGVINLANSNIPVLIFGGRTPISEHSHFGCRNTPIGYGQEMRDQAALIRESVKWDFELRLADQIGEHVDRA WAIASSLPKGPVYLSLPREPLCETFAVDEAALQAGPSQQPVRYAPVREDIARAAEAIACARHPVIFAQRGARTAEGFARL DSLVREWAIPLVEYWGTEVTLSADNPLLAGADPSVWLADADVILVVDSQAPWMIAEGDCRQDCTVIQMGPDPLFSRYPVR GYRADINLAGETDEVFSLLEEALRPLQAARQQHVAERAAYTQSRIQQQKNQRDALLHASQSGAITKPWLSYCLGHLANQH RGRIVSELTTLPQFAGLTHAESYYQEALAGGLGEALPIALGLQLARREELIIAAVGDGSYLFANPAVCHHIAEVMKLPVL VVVGNNGGWGAVAGGTKALYPDGYAARAETIPATAFTTSPDFAAIAASSRAAALSVSRAEDLPGVLEEAVSLIRTRRQSV LVDVQLAR
Specific function: Valine and isoleucine biosynthesis; first step. [C]
COG id: COG0028
COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the TPP enzyme family [H]
Homologues:
Organism=Escherichia coli, GI87081685, Length=584, Percent_Identity=25.3424657534247, Blast_Score=98, Evalue=1e-21, Organism=Saccharomyces cerevisiae, GI6320816, Length=582, Percent_Identity=20.9621993127148, Blast_Score=65, Evalue=3e-11,
Paralogues:
None
Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012846 - InterPro: IPR012000 - InterPro: IPR012001 - InterPro: IPR000399 - InterPro: IPR011766 [H]
Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]
EC number: =2.2.1.6 [H]
Molecular weight: Translated: 61245; Mature: 61114
Theoretical pI: Translated: 5.73; Mature: 5.73
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.6 %Cys (Translated Protein) 1.2 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 1.6 %Cys (Mature Protein) 1.1 %Met (Mature Protein) 2.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MASITLSAAELLLHRLQALDVAYIFINSGTDYPPVIEAWAKARATGQKVPELVICPHENA CCEEEHHHHHHHHHHHHHCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCC AIGMAHGYYLGTGKVQAVMVHTNVGLANAACGVINLANSNIPVLIFGGRTPISEHSHFGC EEEEECCEEEECCCEEEEEEEECCCCCHHCCCEEEECCCCCCEEEECCCCCCCCCCCCCC RNTPIGYGQEMRDQAALIRESVKWDFELRLADQIGEHVDRAWAIASSLPKGPVYLSLPRE CCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEECCCC PLCETFAVDEAALQAGPSQQPVRYAPVREDIARAAEAIACARHPVIFAQRGARTAEGFAR HHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHH LDSLVREWAIPLVEYWGTEVTLSADNPLLAGADPSVWLADADVILVVDSQAPWMIAEGDC HHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCCCEEEECCCEEEEECCCCCEEEECCCC RQDCTVIQMGPDPLFSRYPVRGYRADINLAGETDEVFSLLEEALRPLQAARQQHVAERAA CCCCEEEEECCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH YTQSRIQQQKNQRDALLHASQSGAITKPWLSYCLGHLANQHRGRIVSELTTLPQFAGLTH HHHHHHHHHHCHHHHHEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHH AESYYQEALAGGLGEALPIALGLQLARREELIIAAVGDGSYLFANPAVCHHIAEVMKLPV HHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEECHHHHHHHHHHHCCCE LVVVGNNGGWGAVAGGTKALYPDGYAARAETIPATAFTTSPDFAAIAASSRAAALSVSRA EEEEECCCCCEEECCCCCEECCCCCCCCCCCCCCEEEECCCCCCEEECCCCHHEEEHHHH EDLPGVLEEAVSLIRTRRQSVLVDVQLAR HCCHHHHHHHHHHHHHHHHHEEEEEEECC >Mature Secondary Structure ASITLSAAELLLHRLQALDVAYIFINSGTDYPPVIEAWAKARATGQKVPELVICPHENA CEEEHHHHHHHHHHHHHCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCC AIGMAHGYYLGTGKVQAVMVHTNVGLANAACGVINLANSNIPVLIFGGRTPISEHSHFGC EEEEECCEEEECCCEEEEEEEECCCCCHHCCCEEEECCCCCCEEEECCCCCCCCCCCCCC RNTPIGYGQEMRDQAALIRESVKWDFELRLADQIGEHVDRAWAIASSLPKGPVYLSLPRE CCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEECCCC PLCETFAVDEAALQAGPSQQPVRYAPVREDIARAAEAIACARHPVIFAQRGARTAEGFAR HHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHH LDSLVREWAIPLVEYWGTEVTLSADNPLLAGADPSVWLADADVILVVDSQAPWMIAEGDC HHHHHHHHHHHHHHHCCCEEEEECCCCEEECCCCCEEEECCCEEEEECCCCCEEEECCCC RQDCTVIQMGPDPLFSRYPVRGYRADINLAGETDEVFSLLEEALRPLQAARQQHVAERAA CCCCEEEEECCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH YTQSRIQQQKNQRDALLHASQSGAITKPWLSYCLGHLANQHRGRIVSELTTLPQFAGLTH HHHHHHHHHHCHHHHHEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHH AESYYQEALAGGLGEALPIALGLQLARREELIIAAVGDGSYLFANPAVCHHIAEVMKLPV HHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEECHHHHHHHHHHHCCCE LVVVGNNGGWGAVAGGTKALYPDGYAARAETIPATAFTTSPDFAAIAASSRAAALSVSRA EEEEECCCCCEEECCCCCEECCCCCCCCCCCCCCEEEECCCCCCEEECCCCHHEEEHHHH EDLPGVLEEAVSLIRTRRQSVLVDVQLAR HCCHHHHHHHHHHHHHHHHHEEEEEEECC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 7542800 [H]