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The map label for this gene is fhlA [H]

Identifier: 152971590

GI number: 152971590

Start: 3362541

End: 3364598

Strand: Direct

Name: fhlA [H]

Synonym: KPN_03068

Alternate gene names: 152971590

Gene position: 3362541-3364598 (Clockwise)

Preceding gene: 152971589

Following gene: 152971595

Centisome position: 63.26

GC content: 60.98

Gene sequence:

>2058_bases
ATGAGCGATCTCGGCCAGCAGGGGCTGTTCGATATCACCCGGCTTTTATTACAGCAGCCCGATCTGGCCGCGCTAAGCGA
AACCCTGACCCGTCTGGTGCAACAGTCCGCGCTGGCCGATGAGGCGGCCATCATCCTGTGGAACGCGGGTAATCACCGCG
CGGCCAGGTACGCCTGCGATGAGGCCGGCCATCCGGTAAGCTACGAGGATGAAACCGTCCTGGCCCACGGTCCGGTGCGT
CGCTTACTCTCCCGGCCGGATGCCCTGCATTGCGATCATGAAACCTTTGCCGATACCTGGCCGCAGCTTATCCGCAGCGG
ACTGTATCGTCCGTTCGGTTACTACTCGCTGCTGCCGCTGGCGGCGGACGGGCGGATCTTTGGCGGTTGCGAATTCCTCC
GCCGTGACAACCGGCCGTGGAGCGAGAAAGAGTTCCAGCGGCTGCATACCTTCGCGCAGATCGTCGCCGTCGTCACCGAA
CAGATTCAGAACCGGGTCAGCAATAACGTCGATTACGATCTGCTGTGCCACGAGCGCGACAACTTTCGCATCCTGGTGGC
GATCACCAACGCGGTGCTCTCGCGACTGGATATCGACGAGCTGGTCAGCGAGGTGGCGAAAGAGATCCATCGCTACTTCC
GGATCGACGCCATCAGCGTGGTGCTGCGCAGCGACCGCAAGGGGAAGCTCAACATCTATTCCACGCATTATCTTGACGCC
AGCCACCCGGTTCACGACCAGAGCGAGGTGGATGAAGCCGGCACCCTCACCGAGCGCGTGTTCAAAAGCAAAGAGATGCT
GCTGCTTAATCTGCACGAACATGACACTCTCGCGCCGTATGAGAAAATGCTCTTCGAGATGTGGGGCAATAAGATCCAGA
CCCTGTGCCTGCTGCCGCTGATGTCCGGCAATACCCTGCTCGGGGTGCTTAAGCTGGCGCAGTGCGACGAACAGGTCTTC
ACCACCACCAATCTCAAGCTGCTGCGGCAGATCGCCGAGCGGGTGTCGATCGCTATCGATAACGCCCTCGCCTACCGCGA
AATTCAGCGCCTGAAAGAGCGGCTGGTGGATGAGAATCTCGCCCTCACCGAGCAGCTCAATAATGTCGAGAGCGAATTCG
GGGAGATTATCGGCCGCAGCGAGGCGATGAACAATGTGCTCAAGCAGGTAGAGATGGTGGCGCACAGCGACAGCACCGTG
CTGATCCTCGGCGAAACCGGCACCGGCAAAGAGCTTATCGCCCGCGCTATCCACAACCTCAGCGGACGCAACGGTCGGCG
GATGGTGAAGATGAACTGCGCCGCGATGCCCGCCGGGCTGCTGGAGAGCGATCTGTTCGGCCACGAACGCGGCGCGTTCA
CCGGGGCCAGCGCCCAGCGTATCGGCCGCTTCGAACTGGCGGACAAAAGCTCCCTGTTCCTCGATGAAGTGGGCGATATG
CCGCTGGAACTGCAGCCCAAACTGCTGCGCGTCCTCCAGGAGCAGGAGTTCGAACGTCTGGGGAGCAACAAGCTGATTCA
GACCGACGTTCGATTGATCGCCGCCACCAACCGCGATCTCAAGCAGATGGTTATCGACAGGGAATTCCGCAGCGATCTCT
ACTACCGGCTCAACGTGTTCCCGATCCACCTACCGCCGCTGCGCGAACGTCCCGACGATATCCCGCTGCTGGTGAAGGCG
TTTACCTTTAAAATCGCCCGCCGCATGGGGCGCAATATCGACAGCATTCCGGCGGAAACCCTGCGCACTCTGACGCGTAT
GGAGTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTTATCGAACGGGCGGTATTGCTCACCCGCGGCAACGTACTGCAGC
TCTCCCTGCCGGAGCGGGATATCGTGGAAGCCCCGCGGACGCCGGCGGTCCTGCCTGAGGAAGGTGAGGATGAATATCAA
CTGATCGTCCGGGTGCTGAAGGAGAGCAACGGCGTGGTGGCCGGGCCGAAAGGCGCCGCCCAGCGGCTGGGGCTCAAGCG
CACCACGCTCCTGTCACGCATGAAGCGGCTGGGTATTAATAAAGACGAACTGGTGTAA

Upstream 100 bases:

>100_bases
GGCAATAACCGCTTTATTAACATACTATTATTTGTACCATCATTTTCTTATGCGCCGCGTTGTCGGCGCGTTTTGGACGT
AAGCAATGTCGTATACACCG

Downstream 100 bases:

>100_bases
CCTCGCGCGCTCCCCCTTTCCGCGCCGGTGAGAGGGAGCGCTGGCGCAGCGCGCTATTTATCGCTGGTAAAACCGTTGCT
GGTCGCCAGTTCGGCAAACC

Product: formate hydrogen-lyase transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 685; Mature: 684

Protein sequence:

>685_residues
MSDLGQQGLFDITRLLLQQPDLAALSETLTRLVQQSALADEAAIILWNAGNHRAARYACDEAGHPVSYEDETVLAHGPVR
RLLSRPDALHCDHETFADTWPQLIRSGLYRPFGYYSLLPLAADGRIFGGCEFLRRDNRPWSEKEFQRLHTFAQIVAVVTE
QIQNRVSNNVDYDLLCHERDNFRILVAITNAVLSRLDIDELVSEVAKEIHRYFRIDAISVVLRSDRKGKLNIYSTHYLDA
SHPVHDQSEVDEAGTLTERVFKSKEMLLLNLHEHDTLAPYEKMLFEMWGNKIQTLCLLPLMSGNTLLGVLKLAQCDEQVF
TTTNLKLLRQIAERVSIAIDNALAYREIQRLKERLVDENLALTEQLNNVESEFGEIIGRSEAMNNVLKQVEMVAHSDSTV
LILGETGTGKELIARAIHNLSGRNGRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLDEVGDM
PLELQPKLLRVLQEQEFERLGSNKLIQTDVRLIAATNRDLKQMVIDREFRSDLYYRLNVFPIHLPPLRERPDDIPLLVKA
FTFKIARRMGRNIDSIPAETLRTLTRMEWPGNVRELENVIERAVLLTRGNVLQLSLPERDIVEAPRTPAVLPEEGEDEYQ
LIVRVLKESNGVVAGPKGAAQRLGLKRTTLLSRMKRLGINKDELV

Sequences:

>Translated_685_residues
MSDLGQQGLFDITRLLLQQPDLAALSETLTRLVQQSALADEAAIILWNAGNHRAARYACDEAGHPVSYEDETVLAHGPVR
RLLSRPDALHCDHETFADTWPQLIRSGLYRPFGYYSLLPLAADGRIFGGCEFLRRDNRPWSEKEFQRLHTFAQIVAVVTE
QIQNRVSNNVDYDLLCHERDNFRILVAITNAVLSRLDIDELVSEVAKEIHRYFRIDAISVVLRSDRKGKLNIYSTHYLDA
SHPVHDQSEVDEAGTLTERVFKSKEMLLLNLHEHDTLAPYEKMLFEMWGNKIQTLCLLPLMSGNTLLGVLKLAQCDEQVF
TTTNLKLLRQIAERVSIAIDNALAYREIQRLKERLVDENLALTEQLNNVESEFGEIIGRSEAMNNVLKQVEMVAHSDSTV
LILGETGTGKELIARAIHNLSGRNGRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLDEVGDM
PLELQPKLLRVLQEQEFERLGSNKLIQTDVRLIAATNRDLKQMVIDREFRSDLYYRLNVFPIHLPPLRERPDDIPLLVKA
FTFKIARRMGRNIDSIPAETLRTLTRMEWPGNVRELENVIERAVLLTRGNVLQLSLPERDIVEAPRTPAVLPEEGEDEYQ
LIVRVLKESNGVVAGPKGAAQRLGLKRTTLLSRMKRLGINKDELV
>Mature_684_residues
SDLGQQGLFDITRLLLQQPDLAALSETLTRLVQQSALADEAAIILWNAGNHRAARYACDEAGHPVSYEDETVLAHGPVRR
LLSRPDALHCDHETFADTWPQLIRSGLYRPFGYYSLLPLAADGRIFGGCEFLRRDNRPWSEKEFQRLHTFAQIVAVVTEQ
IQNRVSNNVDYDLLCHERDNFRILVAITNAVLSRLDIDELVSEVAKEIHRYFRIDAISVVLRSDRKGKLNIYSTHYLDAS
HPVHDQSEVDEAGTLTERVFKSKEMLLLNLHEHDTLAPYEKMLFEMWGNKIQTLCLLPLMSGNTLLGVLKLAQCDEQVFT
TTNLKLLRQIAERVSIAIDNALAYREIQRLKERLVDENLALTEQLNNVESEFGEIIGRSEAMNNVLKQVEMVAHSDSTVL
ILGETGTGKELIARAIHNLSGRNGRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMP
LELQPKLLRVLQEQEFERLGSNKLIQTDVRLIAATNRDLKQMVIDREFRSDLYYRLNVFPIHLPPLRERPDDIPLLVKAF
TFKIARRMGRNIDSIPAETLRTLTRMEWPGNVRELENVIERAVLLTRGNVLQLSLPERDIVEAPRTPAVLPEEGEDEYQL
IVRVLKESNGVVAGPKGAAQRLGLKRTTLLSRMKRLGINKDELV

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=689, Percent_Identity=83.8896952104499, Blast_Score=1179, Evalue=0.0,
Organism=Escherichia coli, GI87082117, Length=572, Percent_Identity=50.5244755244755, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI1790437, Length=313, Percent_Identity=46.0063897763578, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1788550, Length=320, Percent_Identity=46.25, Blast_Score=262, Evalue=6e-71,
Organism=Escherichia coli, GI87082152, Length=332, Percent_Identity=41.2650602409639, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI1788905, Length=310, Percent_Identity=43.2258064516129, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI1790299, Length=371, Percent_Identity=40.7008086253369, Blast_Score=238, Evalue=7e-64,
Organism=Escherichia coli, GI1789233, Length=325, Percent_Identity=40.6153846153846, Blast_Score=236, Evalue=4e-63,
Organism=Escherichia coli, GI87081872, Length=232, Percent_Identity=47.4137931034483, Blast_Score=203, Evalue=3e-53,
Organism=Escherichia coli, GI1786524, Length=332, Percent_Identity=38.5542168674699, Blast_Score=198, Evalue=1e-51,
Organism=Escherichia coli, GI1787583, Length=329, Percent_Identity=36.4741641337386, Blast_Score=190, Evalue=3e-49,
Organism=Escherichia coli, GI1789828, Length=263, Percent_Identity=34.9809885931559, Blast_Score=143, Evalue=4e-35,
Organism=Escherichia coli, GI87081858, Length=331, Percent_Identity=32.02416918429, Blast_Score=141, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 77652; Mature: 77521

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLGQQGLFDITRLLLQQPDLAALSETLTRLVQQSALADEAAIILWNAGNHRAARYACD
CCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
EAGHPVSYEDETVLAHGPVRRLLSRPDALHCDHETFADTWPQLIRSGLYRPFGYYSLLPL
CCCCCCCCCCCEEECHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHEEEE
AADGRIFGGCEFLRRDNRPWSEKEFQRLHTFAQIVAVVTEQIQNRVSNNVDYDLLCHERD
CCCCCEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
NFRILVAITNAVLSRLDIDELVSEVAKEIHRYFRIDAISVVLRSDRKGKLNIYSTHYLDA
CCEEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECC
SHPVHDQSEVDEAGTLTERVFKSKEMLLLNLHEHDTLAPYEKMLFEMWGNKIQTLCLLPL
CCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHH
MSGNTLLGVLKLAQCDEQVFTTTNLKLLRQIAERVSIAIDNALAYREIQRLKERLVDENL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ALTEQLNNVESEFGEIIGRSEAMNNVLKQVEMVAHSDSTVLILGETGTGKELIARAIHNL
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHC
SGRNGRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLDEVGDM
CCCCCCEEEEEEHHHCCCHHHHHHHCCCCCCCCCCCHHHHCCCEEECCCCHHHHHHHCCC
PLELQPKLLRVLQEQEFERLGSNKLIQTDVRLIAATNRDLKQMVIDREFRSDLYYRLNVF
CCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHEEECCHHHHHHHHHHHHHHCCEEEEEEE
PIHLPPLRERPDDIPLLVKAFTFKIARRMGRNIDSIPAETLRTLTRMEWPGNVRELENVI
EEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHH
ERAVLLTRGNVLQLSLPERDIVEAPRTPAVLPEEGEDEYQLIVRVLKESNGVVAGPKGAA
HHHHHHCCCCEEEEECCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCHHH
QRLGLKRTTLLSRMKRLGINKDELV
HHCCCHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure 
SDLGQQGLFDITRLLLQQPDLAALSETLTRLVQQSALADEAAIILWNAGNHRAARYACD
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
EAGHPVSYEDETVLAHGPVRRLLSRPDALHCDHETFADTWPQLIRSGLYRPFGYYSLLPL
CCCCCCCCCCCEEECHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHEEEE
AADGRIFGGCEFLRRDNRPWSEKEFQRLHTFAQIVAVVTEQIQNRVSNNVDYDLLCHERD
CCCCCEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
NFRILVAITNAVLSRLDIDELVSEVAKEIHRYFRIDAISVVLRSDRKGKLNIYSTHYLDA
CCEEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECC
SHPVHDQSEVDEAGTLTERVFKSKEMLLLNLHEHDTLAPYEKMLFEMWGNKIQTLCLLPL
CCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHH
MSGNTLLGVLKLAQCDEQVFTTTNLKLLRQIAERVSIAIDNALAYREIQRLKERLVDENL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ALTEQLNNVESEFGEIIGRSEAMNNVLKQVEMVAHSDSTVLILGETGTGKELIARAIHNL
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHC
SGRNGRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLDEVGDM
CCCCCCEEEEEEHHHCCCHHHHHHHCCCCCCCCCCCHHHHCCCEEECCCCHHHHHHHCCC
PLELQPKLLRVLQEQEFERLGSNKLIQTDVRLIAATNRDLKQMVIDREFRSDLYYRLNVF
CCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHEEECCHHHHHHHHHHHHHHCCEEEEEEE
PIHLPPLRERPDDIPLLVKAFTFKIARRMGRNIDSIPAETLRTLTRMEWPGNVRELENVI
EEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHH
ERAVLLTRGNVLQLSLPERDIVEAPRTPAVLPEEGEDEYQLIVRVLKESNGVVAGPKGAA
HHHHHHCCCCEEEEECCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCHHH
QRLGLKRTTLLSRMKRLGINKDELV
HHCCCHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]