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The map label for this gene is emrB [H]

Identifier: 152971542

GI number: 152971542

Start: 3313360

End: 3314898

Strand: Direct

Name: emrB [H]

Synonym: KPN_03015

Alternate gene names: 152971542

Gene position: 3313360-3314898 (Clockwise)

Preceding gene: 152971541

Following gene: 152971543

Centisome position: 62.34

GC content: 61.14

Gene sequence:

>1539_bases
ATGCAACAGCAAAAACCGCTGGAAGGCGCGCAACTGGTCATTATGACCATTGCGCTGTCGCTCGCGACGTTCATGCAGGT
GCTGGATTCCACTATCGCCAACGTGGCGATCCCGACCATTGCCGGCAACCTTGGCTCGTCGCTGAGCCAGGGGACCTGGG
TCATCACCTCGTTCGGGGTCGCTAACGCCATCTCCATCCCGATCACCGGCTGGCTGGCCAAGCGCGTCGGCGAGGTGAAG
CTGTTCCTGTGGTCAACCACCGCCTTCGCCATTGCCTCCTGGGCCTGCGGCGTCTCCAACAGCCTGACGATGCTGATTTT
CTTCCGCGTCATTCAGGGGATCGTCGCCGGGCCGCTGATCCCGCTGTCGCAAAGTCTGCTGCTCAGCAACTATCCCCCGG
CGAAGCGCTCTATCGCTCTGGCGCTGTGGTCAATGACGGTGATTGTCGCGCCCATCTGCGGTCCGATCCTCGGCGGCTAT
ATCAGCGATAATTATCACTGGGGCTGGATCTTCTTTATCAACGTGCCCATTGGCGTGGCGGTGGTGCTGATGACCCTGCA
GACCCTGCGCAACCGGGAAACCAAAACCGAACAGCGGCGGATCGACGGCGTCGGTCTGGCGCTGCTGATCATCGGTATCG
GTAGCCTGCAGGTGATGCTGGACCGGGGTAAAGAGCTGGACTGGTTCTCCTCCAATGAGATTATCATTCTGACCATCGTG
GCGGTGGTGGCGATAAGCTTCCTGATCGTCTGGGAGCTAACCGACGATAACCCGATTGTCGATCTGTCGCTATTTAAGTC
GCGCAACTTCACCATCGGCTGCCTCTGTATCAGTCTGGCCTATATGCTCTACTTCGGCGCCATCGTACTGCTGCCGCAGC
TGTTGCAGGAGGTCTACGGCTACACCGCGACCTGGGCCGGCCTGGCGTCGGCGCCGGTGGGGGTGATCCCGGTCATTCTG
TCGCCGATTATCGGCCGCTTCGCGCATAAGCTCGACATGCGGCGGCTGGTCACCTTCAGCTTTATTATGTACGCGGTCTG
CTTCTACTGGCGCGCCTGGACCTTCGAACCGGGGATGGACTTTGGCGCCTCGGCGTGGCCACAGTTTATCCAGGGCTTCG
CGGTGGCCTGCTTCTTCATGCCGTTGACCACCATCACCCTCTCCGGCCTGCCGCCGGAGCGGCTGGCGGCGGCGTCGAGC
CTGTCGAACTTCACCCGTACCCTTGCCGGCTCCATCGGGACCTCGATCACCACCACCCTGTGGACCAACCGCGAGGCGCT
GCATCATGCGCAGCTCACCGAATCGGTGACCCCCTTTAACCCCAACGCGCAGCAGATATACGATCAGCTGCAGGGCATGG
GAATGACGCAGCAGCAGGCGTCCGGCTGGATAGCGCAGCAGATAACCAACCAGGGGCTGATTATCTCCGCCAACGAAATC
TTCTGGATTTCTGCGGCTATTTTCATCCTGCTGTTGGGGCTGGTGTGGTTCGCCAGACCACCGTTCAGCGCCGGCGGAGG
CGGCGGCGGCGCGCACTAA

Upstream 100 bases:

>100_bases
GGTTTACGAGAGCAATGCCCGCGAAATCGCTCTCGATCCGGTTAATAAGCTGATAGACGAGATCATTCAGGCCAACGCCG
GATAATTCGGGGTGAGTGTA

Downstream 100 bases:

>100_bases
GCCTCACATAAAGGGACCTCCGGGTCCCTTTTTTACGGCGTCAGGTTGTCCAGTTCTCGCTAGTTTCTGTCTCGTTTGCG
AAAGCCCGTCACCCTGCGCT

Product: multidrug resistance protein membrane translocase

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGVANAISIPITGWLAKRVGEVK
LFLWSTTAFAIASWACGVSNSLTMLIFFRVIQGIVAGPLIPLSQSLLLSNYPPAKRSIALALWSMTVIVAPICGPILGGY
ISDNYHWGWIFFINVPIGVAVVLMTLQTLRNRETKTEQRRIDGVGLALLIIGIGSLQVMLDRGKELDWFSSNEIIILTIV
AVVAISFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGVIPVIL
SPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAWTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAAASS
LSNFTRTLAGSIGTSITTTLWTNREALHHAQLTESVTPFNPNAQQIYDQLQGMGMTQQQASGWIAQQITNQGLIISANEI
FWISAAIFILLLGLVWFARPPFSAGGGGGGAH

Sequences:

>Translated_512_residues
MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGVANAISIPITGWLAKRVGEVK
LFLWSTTAFAIASWACGVSNSLTMLIFFRVIQGIVAGPLIPLSQSLLLSNYPPAKRSIALALWSMTVIVAPICGPILGGY
ISDNYHWGWIFFINVPIGVAVVLMTLQTLRNRETKTEQRRIDGVGLALLIIGIGSLQVMLDRGKELDWFSSNEIIILTIV
AVVAISFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGVIPVIL
SPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAWTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAAASS
LSNFTRTLAGSIGTSITTTLWTNREALHHAQLTESVTPFNPNAQQIYDQLQGMGMTQQQASGWIAQQITNQGLIISANEI
FWISAAIFILLLGLVWFARPPFSAGGGGGGAH
>Mature_512_residues
MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGVANAISIPITGWLAKRVGEVK
LFLWSTTAFAIASWACGVSNSLTMLIFFRVIQGIVAGPLIPLSQSLLLSNYPPAKRSIALALWSMTVIVAPICGPILGGY
ISDNYHWGWIFFINVPIGVAVVLMTLQTLRNRETKTEQRRIDGVGLALLIIGIGSLQVMLDRGKELDWFSSNEIIILTIV
AVVAISFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGVIPVIL
SPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAWTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAAASS
LSNFTRTLAGSIGTSITTTLWTNREALHHAQLTESVTPFNPNAQQIYDQLQGMGMTQQQASGWIAQQITNQGLIISANEI
FWISAAIFILLLGLVWFARPPFSAGGGGGGAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=502, Percent_Identity=94.0239043824701, Blast_Score=937, Evalue=0.0,
Organism=Escherichia coli, GI1788710, Length=506, Percent_Identity=62.0553359683794, Blast_Score=634, Evalue=0.0,
Organism=Escherichia coli, GI1790195, Length=423, Percent_Identity=32.6241134751773, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1788392, Length=404, Percent_Identity=29.2079207920792, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI87081983, Length=425, Percent_Identity=26.8235294117647, Blast_Score=122, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6322958, Length=535, Percent_Identity=21.6822429906542, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319770, Length=313, Percent_Identity=26.1980830670927, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=31.3333333333333, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=137, Percent_Identity=29.1970802919708, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55667; Mature: 55667

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCEEEEEECCC
ANAISIPITGWLAKRVGEVKLFLWSTTAFAIASWACGVSNSLTMLIFFRVIQGIVAGPLI
CCEEECCHHHHHHHCCCCEEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
PLSQSLLLSNYPPAKRSIALALWSMTVIVAPICGPILGGYISDNYHWGWIFFINVPIGVA
CCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECHHHHH
VVLMTLQTLRNRETKTEQRRIDGVGLALLIIGIGSLQVMLDRGKELDWFSSNEIIILTIV
HHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCHHHHHHHCCCCCCCCCCCCEEHHHHH
AVVAISFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYG
HHHHHHHHEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YTATWAGLASAPVGVIPVILSPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAWTFEPGMD
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAAASSLSNFTRTLAGSIGTSITTTL
CCCHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
WTNREALHHAQLTESVTPFNPNAQQIYDQLQGMGMTQQQASGWIAQQITNQGLIISANEI
ECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHCCCCEEEECCHH
FWISAAIFILLLGLVWFARPPFSAGGGGGGAH
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCEEEEEECCC
ANAISIPITGWLAKRVGEVKLFLWSTTAFAIASWACGVSNSLTMLIFFRVIQGIVAGPLI
CCEEECCHHHHHHHCCCCEEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
PLSQSLLLSNYPPAKRSIALALWSMTVIVAPICGPILGGYISDNYHWGWIFFINVPIGVA
CCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECHHHHH
VVLMTLQTLRNRETKTEQRRIDGVGLALLIIGIGSLQVMLDRGKELDWFSSNEIIILTIV
HHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCHHHHHHHCCCCCCCCCCCCEEHHHHH
AVVAISFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYG
HHHHHHHHEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YTATWAGLASAPVGVIPVILSPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAWTFEPGMD
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAAASSLSNFTRTLAGSIGTSITTTL
CCCHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
WTNREALHHAQLTESVTPFNPNAQQIYDQLQGMGMTQQQASGWIAQQITNQGLIISANEI
ECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHCCCCEEEECCHH
FWISAAIFILLLGLVWFARPPFSAGGGGGGAH
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]