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The map label for this gene is amiA [H]

Identifier: 152971309

GI number: 152971309

Start: 3045688

End: 3046560

Strand: Direct

Name: amiA [H]

Synonym: KPN_02778

Alternate gene names: 152971309

Gene position: 3045688-3046560 (Clockwise)

Preceding gene: 152971299

Following gene: 152971310

Centisome position: 57.3

GC content: 57.5

Gene sequence:

>873_bases
ATGAGCACTTTTAAACCCTTAAAAATACTTGCTTCGCGCCGTCAGGTGCTGAAAGCCGGGCTGGCTGCGATGACGCTGAG
CGGCGTCGCCTCACAGGTCAGCGCAAAAGAACAGCCACTGAAAACGTCAAATGGACATAGCAAACCCGCCGCCAAAAAGG
CCGGAGGTCGCCGGATTGTAATGTTAGATCCGGGCCACGGCGGCATCGATACCGGCGCTATCGGCCATAACGGCTCGAAA
GAGAAGCATGTGGTGTTGGCGATCGCTAAGAACGTGCGCAGCATTTTGCGCAGCAACGGCATCGACGCCCGCCTGACCCG
CACCGGGGATACCTTTATCCCGCTGTACGACCGCGTGGAGATCGCCCATCAGCACGGCGCCGATCTGTTTATGTCAATCC
ACGCCGATGGCTTCACCAACCCGAGCGCCGCCGGCGCCTCGGTGTTCGCCCTGTCAAACCGCGGCGCCAGTAGCGCCATG
GCGAAATACCTCTCCGATCGCGAAAACCGCGCCGATGAGGTCGCCGGTAAGAAAGCGACGGATAAAGACCATCTGCTGCA
GCAGGTACTGTTCGATCTGGTGCAGACCGACACCATCAAAAACAGCCTGACGCTGGGCTCGCATATCCTGAAAAAGATCA
AGCCGGTACATAAGCTGCACAGCCGCAATACCGAGCAGGCGGCGTTCGTGGTGCTGAAGTCACCATCCATACCGTCCGTG
CTGGTGGAAACCTCGTTTATCACCAACCCGAACGAGGAGAAACTGCTCGGCACCACCGCCTTCCGGCAGAAAATCGCCAC
CGCGATTGCCAACGGCATTATCAGCTATTTCCACTGGTTCGATAACCAGAAAGCCCATTCGAAGAGACGTTAA

Upstream 100 bases:

>100_bases
TATTGAAATGAAAAGTAAAACAATTCTTATTTTGAATGGTCGTAACGGCAAACGCGATACGATATAACATTGGACATCAC
TTGTTTACAATTCAGGCCGA

Downstream 100 bases:

>100_bases
TGAAACCCGATGCCGCGCAGGTAAAAACTTTTCTGCTCCAGCTCCAGGACAGCCTCTGTCAGCAGCTGAGCGCCGTGGAT
GGCGCGCCGTTTATTGAAGA

Product: N-acetylmuramoyl-l-alanine amidase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MSTFKPLKILASRRQVLKAGLAAMTLSGVASQVSAKEQPLKTSNGHSKPAAKKAGGRRIVMLDPGHGGIDTGAIGHNGSK
EKHVVLAIAKNVRSILRSNGIDARLTRTGDTFIPLYDRVEIAHQHGADLFMSIHADGFTNPSAAGASVFALSNRGASSAM
AKYLSDRENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSIPSV
LVETSFITNPNEEKLLGTTAFRQKIATAIANGIISYFHWFDNQKAHSKRR

Sequences:

>Translated_290_residues
MSTFKPLKILASRRQVLKAGLAAMTLSGVASQVSAKEQPLKTSNGHSKPAAKKAGGRRIVMLDPGHGGIDTGAIGHNGSK
EKHVVLAIAKNVRSILRSNGIDARLTRTGDTFIPLYDRVEIAHQHGADLFMSIHADGFTNPSAAGASVFALSNRGASSAM
AKYLSDRENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSIPSV
LVETSFITNPNEEKLLGTTAFRQKIATAIANGIISYFHWFDNQKAHSKRR
>Mature_289_residues
STFKPLKILASRRQVLKAGLAAMTLSGVASQVSAKEQPLKTSNGHSKPAAKKAGGRRIVMLDPGHGGIDTGAIGHNGSKE
KHVVLAIAKNVRSILRSNGIDARLTRTGDTFIPLYDRVEIAHQHGADLFMSIHADGFTNPSAAGASVFALSNRGASSAMA
KYLSDRENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSIPSVL
VETSFITNPNEEKLLGTTAFRQKIATAIANGIISYFHWFDNQKAHSKRR

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm. Note=Distributed throughout the periplasm in all cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=290, Percent_Identity=90, Blast_Score=536, Evalue=1e-154,
Organism=Escherichia coli, GI87082163, Length=236, Percent_Identity=47.0338983050847, Blast_Score=215, Evalue=2e-57,
Organism=Escherichia coli, GI1790611, Length=244, Percent_Identity=38.5245901639344, Blast_Score=171, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 31369; Mature: 31238

Theoretical pI: Translated: 10.91; Mature: 10.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFKPLKILASRRQVLKAGLAAMTLSGVASQVSAKEQPLKTSNGHSKPAAKKAGGRRIV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCEEE
MLDPGHGGIDTGAIGHNGSKEKHVVLAIAKNVRSILRSNGIDARLTRTGDTFIPLYDRVE
EECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCEEEEECCCCEECHHHHHH
IAHQHGADLFMSIHADGFTNPSAAGASVFALSNRGASSAMAKYLSDRENRADEVAGKKAT
HHHHCCCEEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCC
DKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSIPSV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCE
LVETSFITNPNEEKLLGTTAFRQKIATAIANGIISYFHWFDNQKAHSKRR
EEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCC
>Mature Secondary Structure 
STFKPLKILASRRQVLKAGLAAMTLSGVASQVSAKEQPLKTSNGHSKPAAKKAGGRRIV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCEEE
MLDPGHGGIDTGAIGHNGSKEKHVVLAIAKNVRSILRSNGIDARLTRTGDTFIPLYDRVE
EECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCEEEEECCCCEECHHHHHH
IAHQHGADLFMSIHADGFTNPSAAGASVFALSNRGASSAMAKYLSDRENRADEVAGKKAT
HHHHCCCEEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCC
DKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSIPSV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCE
LVETSFITNPNEEKLLGTTAFRQKIATAIANGIISYFHWFDNQKAHSKRR
EEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503; 1903834 [H]