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The map label for this gene is ackA [H]

Identifier: 152971225

GI number: 152971225

Start: 2957929

End: 2959131

Strand: Direct

Name: ackA [H]

Synonym: KPN_02687

Alternate gene names: 152971225

Gene position: 2957929-2959131 (Clockwise)

Preceding gene: 152971219

Following gene: 152971226

Centisome position: 55.65

GC content: 55.94

Gene sequence:

>1203_bases
ATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGCTCCTCTCTGAAATTTGCTATCCTTGACGCCGTGAACGGCGA
CGAATATCTGTCCGGTTTAGCGGAATGCTTCCACCTGCCGGAAGCCCGTATCAAATGGAAAATGGACGGCAGCAAACAAG
AAGCCGAGTTAGGCGCGGGCGCCGCGCACAGTGAAGCGCTGAACTTTATCGTTAACACTATTCTGGCACAAAAACCAGAA
CTGTCTGCTCAGCTGACCGCAATCGGCCACCGTATCGTACACGGCGGTGAAAAATACACCAGCTCCGTGGTGATCGACGA
GTCCGTAATTCAGGGTATTAAAGACGCCGCGTCTTTCGCACCGCTGCACAACCCGGCACACCTGATCGGCATCGCTGAAG
CGCTGAAATCCTTCCCGCACCTGAAAGACAAAAACGTGGCTGTATTCGATACGGCGTTCCATCAGACCATGCCGGAAGAA
TCCTATCTCTACGCCCTGCCGTACAGCCTGTACAAAGAGCACGGCGTTCGTCGTTACGGCGCGCACGGCACCAGCCACTT
CTACGTGACTCAGGAAGCCGCTAAGATCCTCAACAAGCCGGTGGAAGAGCTGAACATCATCACCTGCCACCTCGGCAACG
GTGGTTCTGTTTCCGCTATCCGTAACGGTAAATGCGTTGACACCTCCATGGGTCTGACCCCGCTGGAAGGCCTGGTGATG
GGTACCCGCTCCGGTGACATCGATCCGGCCATCATCTTCCATCTGCACGACACCCTGGGCATGAGCGTTGACGCGATCAA
CAAAATGCTGACCAAAGAGTCCGGCCTGCTGGGTCTGACCGAAGTCACCAGCGACTGCCGCTACGTGGAAGACAACTATC
AGGAAAAAGCCGACGCTAAACGCGCAATGGACGTTTACTGCCACCGCCTGGCGAAATACATCGGTTCCTACACCGCGCTG
ATGGACGGCCGTCTTGACGCCGTTATTTTCACCGGTGGTATCGGTGAAAACGCCGCGATGGTGCGTGAGCTGTCGCTGGG
CAAACTGGGCGTACTGGGCTTTGAAGTTGACCATGAGCGTAACCTTGCTGCCCGCTTCGGCAAGTCTGGCTTCATCAACA
AAGAAGGCACCCGTCCTGCCGTGGTTATCCCGACCAACGAAGAGCTGGTCATCGCGCAAGACGCCAGCCGTCTGACCGCA
TAA

Upstream 100 bases:

>100_bases
GAACAAGGCATGATGTTAATCATAAATGTCGAGGTCATCCTGCGCTACGCTAATGACACACTGACGTTTTTTTAGCCACG
TATCATAAAAAGGTACTTCC

Downstream 100 bases:

>100_bases
TTCCACATCGCCAGCCCTGACCCGCAATAATTCGAGCTGCAGGAAGGCGGCGACACAGCAAAACCCCGGCCGCGTACAGC
GCGTACGCCACCGGGGTGAG

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSSKLVLVLNCGSSSLKFAILDAVNGDEYLSGLAECFHLPEARIKWKMDGSKQEAELGAGAAHSEALNFIVNTILAQKPE
LSAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDAASFAPLHNPAHLIGIAEALKSFPHLKDKNVAVFDTAFHQTMPEE
SYLYALPYSLYKEHGVRRYGAHGTSHFYVTQEAAKILNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVM
GTRSGDIDPAIIFHLHDTLGMSVDAINKMLTKESGLLGLTEVTSDCRYVEDNYQEKADAKRAMDVYCHRLAKYIGSYTAL
MDGRLDAVIFTGGIGENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA

Sequences:

>Translated_400_residues
MSSKLVLVLNCGSSSLKFAILDAVNGDEYLSGLAECFHLPEARIKWKMDGSKQEAELGAGAAHSEALNFIVNTILAQKPE
LSAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDAASFAPLHNPAHLIGIAEALKSFPHLKDKNVAVFDTAFHQTMPEE
SYLYALPYSLYKEHGVRRYGAHGTSHFYVTQEAAKILNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVM
GTRSGDIDPAIIFHLHDTLGMSVDAINKMLTKESGLLGLTEVTSDCRYVEDNYQEKADAKRAMDVYCHRLAKYIGSYTAL
MDGRLDAVIFTGGIGENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA
>Mature_399_residues
SSKLVLVLNCGSSSLKFAILDAVNGDEYLSGLAECFHLPEARIKWKMDGSKQEAELGAGAAHSEALNFIVNTILAQKPEL
SAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDAASFAPLHNPAHLIGIAEALKSFPHLKDKNVAVFDTAFHQTMPEES
YLYALPYSLYKEHGVRRYGAHGTSHFYVTQEAAKILNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVMG
TRSGDIDPAIIFHLHDTLGMSVDAINKMLTKESGLLGLTEVTSDCRYVEDNYQEKADAKRAMDVYCHRLAKYIGSYTALM
DGRLDAVIFTGGIGENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=400, Percent_Identity=96.25, Blast_Score=800, Evalue=0.0,
Organism=Escherichia coli, GI145693184, Length=396, Percent_Identity=43.6868686868687, Blast_Score=311, Evalue=4e-86,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43275; Mature: 43144

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKLVLVLNCGSSSLKFAILDAVNGDEYLSGLAECFHLPEARIKWKMDGSKQEAELGAG
CCCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHCCCCHHEEEEECCCCHHHHHCCC
AAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDAASFA
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHEEEECHHHHHHHHHHHHHC
PLHNPAHLIGIAEALKSFPHLKDKNVAVFDTAFHQTMPEESYLYALPYSLYKEHGVRRYG
CCCCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCCEEEECCHHHHHHCCCEECC
AHGTSHFYVTQEAAKILNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVM
CCCCEEEEEEHHHHHHHHCCHHHCEEEEEEECCCCCEEEECCCEEEECCCCCCCCCCEEE
GTRSGDIDPAIIFHLHDTLGMSVDAINKMLTKESGLLGLTEVTSDCRYVEDNYQEKADAK
ECCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHCCCCEEHHHHHCCCHHHHHHHHHHHHHH
RAMDVYCHRLAKYIGSYTALMDGRLDAVIFTGGIGENAAMVRELSLGKLGVLGFEVDHER
HHHHHHHHHHHHHHCCHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCEEEEEEEECCCC
NLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA
CHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEECCHHCCC
>Mature Secondary Structure 
SSKLVLVLNCGSSSLKFAILDAVNGDEYLSGLAECFHLPEARIKWKMDGSKQEAELGAG
CCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHCCCCHHEEEEECCCCHHHHHCCC
AAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDAASFA
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHEEEECHHHHHHHHHHHHHC
PLHNPAHLIGIAEALKSFPHLKDKNVAVFDTAFHQTMPEESYLYALPYSLYKEHGVRRYG
CCCCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCCEEEECCHHHHHHCCCEECC
AHGTSHFYVTQEAAKILNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVM
CCCCEEEEEEHHHHHHHHCCHHHCEEEEEEECCCCCEEEECCCEEEECCCCCCCCCCEEE
GTRSGDIDPAIIFHLHDTLGMSVDAINKMLTKESGLLGLTEVTSDCRYVEDNYQEKADAK
ECCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHCCCCEEHHHHHCCCHHHHHHHHHHHHHH
RAMDVYCHRLAKYIGSYTALMDGRLDAVIFTGGIGENAAMVRELSLGKLGVLGFEVDHER
HHHHHHHHHHHHHHCCHHHHHCCCEEEEEEECCCCCCHHHHHHHCCCCEEEEEEEECCCC
NLAARFGKSGFINKEGTRPAVVIPTNEELVIAQDASRLTA
CHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]