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The map label for this gene is hisF [H]

Identifier: 152971020

GI number: 152971020

Start: 2705413

End: 2706189

Strand: Direct

Name: hisF [H]

Synonym: KPN_02481

Alternate gene names: 152971020

Gene position: 2705413-2706189 (Clockwise)

Preceding gene: 152971019

Following gene: 152971021

Centisome position: 50.9

GC content: 60.23

Gene sequence:

>777_bases
ATGCTGGCAAAACGGATAATCCCCTGCCTCGACGTCCGCGACGGCCAGGTAGTGAAAGGCGTGCAGTTCCGTAATCACGA
GATCATTGGCGACATCGTCCCGCTGGCCAAGCGCTATGCCGACGAAGGGGCCGACGAACTGGTATTCTATGATATCACCG
CCTCCAGCGACGGTCGGGTGGTCGACAAGAGCTGGGTGTCGCGGGTAGCGGAAGTGATTGATATTCCTTTCTGCGTGGCG
GGCGGGATCAAGTCGCTGGAAGACGCGGCGCAGATCCTCTCCTTCGGCGCCGATAAGATCTCGATTAACTCCCCTGCTCT
CGCCGACCCGACGCTGATCACCCGCCTTGCCGACCGCTTTGGCGTGCAGTGTATCGTGGTGGGGATTGATACCTGGTTTG
ATGCAGAGACCGGCAAGTATCACGTCAACCAGTATACCGGCGACGAGAGCCGCACCCGGGTGACGCAGTGGGAAACCCTC
GACTGGGTGGAGGAAGTGCAGAAGCGCGGCGCAGGCGAGATCGTTCTCAATATGATGAACCAGGACGGGGTGCGCAACGG
TTACGACCTGCAGCAGCTGGCGAAGGTTCGCGCGGTGTGCCACGTCCCGCTGATCGCCTCCGGCGGCGCGGGCACTATGG
AACACTTCCTCGAAGCCTTCCGCGACGCCGACGTTGACGGCGCGCTGGCGGCTTCGGTATTCCACAAACAGATTATTAAT
ATTGGCGAACTGAAAGCGTACCTGGCGGCACAGGGCGTGGAGATAAGGGTATGTTGA

Upstream 100 bases:

>100_bases
ATCTCAAAGATATTGCCGCGCTGCGCGGCACCGGGGTGCGCGGCGTGATTGTTGGCCGCGCGCTGCTGGAAGGTAAATTC
AACGTAACGGAGGCCATTCA

Downstream 100 bases:

>100_bases
CTGAACAGCTGGACTGGGAAAAAACGGACGGCATGATGCCGGCCATCGTGCAGCACGCCGTATCCGGCGAAGTGCTGATG
CACGGCTACATGAACAAGGA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYADEGADELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVA
GGIKSLEDAAQILSFGADKISINSPALADPTLITRLADRFGVQCIVVGIDTWFDAETGKYHVNQYTGDESRTRVTQWETL
DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLQQLAKVRAVCHVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
IGELKAYLAAQGVEIRVC

Sequences:

>Translated_258_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYADEGADELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVA
GGIKSLEDAAQILSFGADKISINSPALADPTLITRLADRFGVQCIVVGIDTWFDAETGKYHVNQYTGDESRTRVTQWETL
DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLQQLAKVRAVCHVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
IGELKAYLAAQGVEIRVC
>Mature_258_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYADEGADELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVA
GGIKSLEDAAQILSFGADKISINSPALADPTLITRLADRFGVQCIVVGIDTWFDAETGKYHVNQYTGDESRTRVTQWETL
DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLQQLAKVRAVCHVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
IGELKAYLAAQGVEIRVC

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=96.5116279069768, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI87082028, Length=228, Percent_Identity=25.4385964912281, Blast_Score=66, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319725, Length=318, Percent_Identity=31.1320754716981, Blast_Score=128, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 28265; Mature: 28265

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYADEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCE
VDKSWVSRVAEVIDIPFCVAGGIKSLEDAAQILSFGADKISINSPALADPTLITRLADRF
ECHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDTWFDAETGKYHVNQYTGDESRTRVTQWETLDWVEEVQKRGAGEIVLNMMN
CCEEEEEEECCCCCCCCCCEEEEEECCCCHHHEEHHHHHHHHHHHHHHCCCCCEEHHHHC
QDGVRNGYDLQQLAKVRAVCHVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IGELKAYLAAQGVEIRVC
HHHHHHHHHHCCCEEEEC
>Mature Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYADEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCE
VDKSWVSRVAEVIDIPFCVAGGIKSLEDAAQILSFGADKISINSPALADPTLITRLADRF
ECHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDTWFDAETGKYHVNQYTGDESRTRVTQWETLDWVEEVQKRGAGEIVLNMMN
CCEEEEEEECCCCCCCCCCEEEEEECCCCHHHEEHHHHHHHHHHHHHHCCCCCEEHHHHC
QDGVRNGYDLQQLAKVRAVCHVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IGELKAYLAAQGVEIRVC
HHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA