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The map label for this gene is yebU [H]

Identifier: 152970898

GI number: 152970898

Start: 2576551

End: 2577939

Strand: Direct

Name: yebU [H]

Synonym: KPN_02351

Alternate gene names: 152970898

Gene position: 2576551-2577939 (Clockwise)

Preceding gene: 152970897

Following gene: 152970899

Centisome position: 48.48

GC content: 62.49

Gene sequence:

>1389_bases
ATGCGCGCGGCGATGCCAGCGCATCTCTCTTTCGACGACTTTATCGCCGCCTGTCAGCGCCCGCTACGCCGCAGTATTCG
CGTCAATACGCTGAAGATTAGCGTCGATGATTTCCTCTCTCTGGTTGCCCCCTACGGCTGGCAGCTCGCGCCGGTGCCCT
GGTGCGCAGAGGGATTCTGGATCGAGCGAGAGGATGACGACGCGCTGCCGCTGGGCAGTACCGCCGAACATCTCAGCGGG
CTGTTCTATATTCAGGAAGCCAGTTCGATGCTGCCGGTCGCCGCCCTGTTCGCCGATAACCGGCAGCCCGAACGGGTGAT
GGACGTTGCCGCCGCCCCGGGATCGAAAACCACCCAAATTGCAGCCCGAATGGGCAACGCGGGCGGCATTCTCGCCAATG
AATTCTCCGCCAGCCGGGTGAAGGTGCTGCATGCCAACATCAGCCGCTGCGGCATCAGCAACGTCGCTCTGACCCACTTC
GACGGCCGGGTTTTTGGCGCGGCCTTGCCCGAAACCTTCGATGCCATCCTGCTTGACGCCCCCTGCTCCGGCGAAGGGGT
TGTACGTAAAGACGCCGACGCGCTGAAAAACTGGTCGCCGGAGAGCAACCTCGACATCGCCGCCACTCAGCGCGAGCTTA
TCGACAGCGCCTTCCATGCTCTGCGCCCTGGCGGGACGCTGGTGTATTCCACCTGCACCCTGAATCGTGAAGAAAATCAA
TCGGTCATTGAGTGGCTGCTGTCCCGCTATCCGCAGGCGGTGGAGATCCTGCCGCTGGGAGAGTTGTTTCCCGGCGCAGC
CGACGCCTTAACCGCGGAAGGTTTTCTGCACGTTTTTCCGCAAATTTACGATTGCGAAGGCTTCTTTGTCGCAAGGTTGC
GCAAAACTGCGGCGATCGATCCGCTCCCGGCGCCAGGCTATAAGGTCGGTAAATTCCCGTTTACGCCGCTGAAGGACCGG
GAGGCCGCCGCGGTAACTGCCGCCGCCAGGGCTGTTGGCCTGGAGTGGGACGCAGGCCATACGCTGTGGCAGCGGGATAA
AGAGCTGTGGCTGTTTCCGCTGGCTTTGGAGCCCCTGTTCGGCAAGGTGCGCTTTTCTCGCATCGGCGTGCGCCTGGCGG
AGCTGCACAATAAGGGGTACCGCTGGCAGCATGAGGCGGTTATCGCCTTTGCCGCGCCGCAGCGGGCCTTCGAGCTGTCG
CAGGAGGAAGCGGAGGAATGGTATCGCGGGCGCGACGTCTATCCGCAAACCGCGCCTGGGCAGGATGAAACCATTGTGAC
CTTCCAGGGCGTCCCGCTGGGCCTGGCGAAGCGCGTCGGCTCCCGACTGAAGAACAGCTATCCGCGCGAACTGGTGCGCG
ATGGAAAACTTTTTGCCGGCAAGGTGTGA

Upstream 100 bases:

>100_bases
GCGCCGGGGCGTTTGTGCTAAACTGCGCACCCTTTTTTTCTGTGGTGCGCTGCCCGTGGCTGAAAATACTGTCTATTTCC
CTGAAGCGTTTCTCGCTCAA

Downstream 100 bases:

>100_bases
GGCCGCGCAAAAAATGCGCACTTTTTGACTGGCGCTTTTCGCCCCATTGACTACGCTGAAAAATAACGGCCATAACTGGT
CGTACCACAACTCAGCTGAA

Product: rRNA (cytosine-C(5)-)-methyltransferase RsmF

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MRAAMPAHLSFDDFIAACQRPLRRSIRVNTLKISVDDFLSLVAPYGWQLAPVPWCAEGFWIEREDDDALPLGSTAEHLSG
LFYIQEASSMLPVAALFADNRQPERVMDVAAAPGSKTTQIAARMGNAGGILANEFSASRVKVLHANISRCGISNVALTHF
DGRVFGAALPETFDAILLDAPCSGEGVVRKDADALKNWSPESNLDIAATQRELIDSAFHALRPGGTLVYSTCTLNREENQ
SVIEWLLSRYPQAVEILPLGELFPGAADALTAEGFLHVFPQIYDCEGFFVARLRKTAAIDPLPAPGYKVGKFPFTPLKDR
EAAAVTAAARAVGLEWDAGHTLWQRDKELWLFPLALEPLFGKVRFSRIGVRLAELHNKGYRWQHEAVIAFAAPQRAFELS
QEEAEEWYRGRDVYPQTAPGQDETIVTFQGVPLGLAKRVGSRLKNSYPRELVRDGKLFAGKV

Sequences:

>Translated_462_residues
MRAAMPAHLSFDDFIAACQRPLRRSIRVNTLKISVDDFLSLVAPYGWQLAPVPWCAEGFWIEREDDDALPLGSTAEHLSG
LFYIQEASSMLPVAALFADNRQPERVMDVAAAPGSKTTQIAARMGNAGGILANEFSASRVKVLHANISRCGISNVALTHF
DGRVFGAALPETFDAILLDAPCSGEGVVRKDADALKNWSPESNLDIAATQRELIDSAFHALRPGGTLVYSTCTLNREENQ
SVIEWLLSRYPQAVEILPLGELFPGAADALTAEGFLHVFPQIYDCEGFFVARLRKTAAIDPLPAPGYKVGKFPFTPLKDR
EAAAVTAAARAVGLEWDAGHTLWQRDKELWLFPLALEPLFGKVRFSRIGVRLAELHNKGYRWQHEAVIAFAAPQRAFELS
QEEAEEWYRGRDVYPQTAPGQDETIVTFQGVPLGLAKRVGSRLKNSYPRELVRDGKLFAGKV
>Mature_462_residues
MRAAMPAHLSFDDFIAACQRPLRRSIRVNTLKISVDDFLSLVAPYGWQLAPVPWCAEGFWIEREDDDALPLGSTAEHLSG
LFYIQEASSMLPVAALFADNRQPERVMDVAAAPGSKTTQIAARMGNAGGILANEFSASRVKVLHANISRCGISNVALTHF
DGRVFGAALPETFDAILLDAPCSGEGVVRKDADALKNWSPESNLDIAATQRELIDSAFHALRPGGTLVYSTCTLNREENQ
SVIEWLLSRYPQAVEILPLGELFPGAADALTAEGFLHVFPQIYDCEGFFVARLRKTAAIDPLPAPGYKVGKFPFTPLKDR
EAAAVTAAARAVGLEWDAGHTLWQRDKELWLFPLALEPLFGKVRFSRIGVRLAELHNKGYRWQHEAVIAFAAPQRAFELS
QEEAEEWYRGRDVYPQTAPGQDETIVTFQGVPLGLAKRVGSRLKNSYPRELVRDGKLFAGKV

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=296, Percent_Identity=35.8108108108108, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI76150623, Length=296, Percent_Identity=35.8108108108108, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI11545785, Length=176, Percent_Identity=39.2045454545455, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI32698918, Length=165, Percent_Identity=36.3636363636364, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI39995082, Length=206, Percent_Identity=31.5533980582524, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI301336155, Length=206, Percent_Identity=31.5533980582524, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI40316918, Length=228, Percent_Identity=30.2631578947368, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI23199998, Length=317, Percent_Identity=28.7066246056782, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI8922322, Length=317, Percent_Identity=28.7066246056782, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI270288816, Length=317, Percent_Identity=28.7066246056782, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI270288818, Length=317, Percent_Identity=28.7066246056782, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI87081985, Length=463, Percent_Identity=80.5615550755939, Blast_Score=775, Evalue=0.0,
Organism=Escherichia coli, GI2367212, Length=273, Percent_Identity=29.6703296703297, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17536757, Length=299, Percent_Identity=34.4481605351171, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI25143471, Length=193, Percent_Identity=31.0880829015544, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71998419, Length=311, Percent_Identity=27.9742765273312, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI115534021, Length=227, Percent_Identity=29.5154185022026, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324268, Length=288, Percent_Identity=37.1527777777778, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6319447, Length=206, Percent_Identity=31.0679611650485, Blast_Score=107, Evalue=3e-24,
Organism=Drosophila melanogaster, GI22024126, Length=306, Percent_Identity=34.640522875817, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI21355201, Length=208, Percent_Identity=33.1730769230769, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24668781, Length=180, Percent_Identity=35.5555555555556, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI21356513, Length=180, Percent_Identity=35.5555555555556, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19920494, Length=177, Percent_Identity=28.2485875706215, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 50889; Mature: 50889

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAAMPAHLSFDDFIAACQRPLRRSIRVNTLKISVDDFLSLVAPYGWQLAPVPWCAEGFW
CCCCCCCCCCHHHHHHHHHHHHHHCEEEEEEEEEHHHHHHHHCCCCCEECCCCCCCCCEE
IEREDDDALPLGSTAEHLSGLFYIQEASSMLPVAALFADNRQPERVMDVAAAPGSKTTQI
EECCCCCCCCCCCCHHHHHHEEEEECCCCCCHHHHHHHCCCCHHHHHHHHCCCCCCHHHH
AARMGNAGGILANEFSASRVKVLHANISRCGISNVALTHFDGRVFGAALPETFDAILLDA
HHHCCCCCCEEECCCCCCEEEEEECCHHHCCCCCEEEEEECCEEEECCCCCCCCEEEEEC
PCSGEGVVRKDADALKNWSPESNLDIAATQRELIDSAFHALRPGGTLVYSTCTLNREENQ
CCCCCCCEECCHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCH
SVIEWLLSRYPQAVEILPLGELFPGAADALTAEGFLHVFPQIYDCEGFFVARLRKTAAID
HHHHHHHHHCCCEEEEEECHHHCCCCHHHHHHCCHHHHCCHHCCCCCHHHHHHHHHHCCC
PLPAPGYKVGKFPFTPLKDREAAAVTAAARAVGLEWDAGHTLWQRDKELWLFPLALEPLF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEEEEHHHHH
GKVRFSRIGVRLAELHNKGYRWQHEAVIAFAAPQRAFELSQEEAEEWYRGRDVYPQTAPG
HHHHHHHHHHHHHHHHCCCCCEECEEEEEEECCHHHHHCCHHHHHHHHCCCCCCCCCCCC
QDETIVTFQGVPLGLAKRVGSRLKNSYPRELVRDGKLFAGKV
CCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHCCCEEECCC
>Mature Secondary Structure
MRAAMPAHLSFDDFIAACQRPLRRSIRVNTLKISVDDFLSLVAPYGWQLAPVPWCAEGFW
CCCCCCCCCCHHHHHHHHHHHHHHCEEEEEEEEEHHHHHHHHCCCCCEECCCCCCCCCEE
IEREDDDALPLGSTAEHLSGLFYIQEASSMLPVAALFADNRQPERVMDVAAAPGSKTTQI
EECCCCCCCCCCCCHHHHHHEEEEECCCCCCHHHHHHHCCCCHHHHHHHHCCCCCCHHHH
AARMGNAGGILANEFSASRVKVLHANISRCGISNVALTHFDGRVFGAALPETFDAILLDA
HHHCCCCCCEEECCCCCCEEEEEECCHHHCCCCCEEEEEECCEEEECCCCCCCCEEEEEC
PCSGEGVVRKDADALKNWSPESNLDIAATQRELIDSAFHALRPGGTLVYSTCTLNREENQ
CCCCCCCEECCHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCH
SVIEWLLSRYPQAVEILPLGELFPGAADALTAEGFLHVFPQIYDCEGFFVARLRKTAAID
HHHHHHHHHCCCEEEEEECHHHCCCCHHHHHHCCHHHHCCHHCCCCCHHHHHHHHHHCCC
PLPAPGYKVGKFPFTPLKDREAAAVTAAARAVGLEWDAGHTLWQRDKELWLFPLALEPLF
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEEEEHHHHH
GKVRFSRIGVRLAELHNKGYRWQHEAVIAFAAPQRAFELSQEEAEEWYRGRDVYPQTAPG
HHHHHHHHHHHHHHHHCCCCCEECEEEEEEECCHHHHHCCHHHHHHHHCCCCCCCCCCCC
QDETIVTFQGVPLGLAKRVGSRLKNSYPRELVRDGKLFAGKV
CCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA