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The map label for this gene is narX [H]

Identifier: 152970763

GI number: 152970763

Start: 2428392

End: 2430170

Strand: Direct

Name: narX [H]

Synonym: KPN_02216

Alternate gene names: 152970763

Gene position: 2428392-2430170 (Clockwise)

Preceding gene: 152970757

Following gene: 152970764

Centisome position: 45.69

GC content: 60.71

Gene sequence:

>1779_bases
ATGTTAAAACGTCTTTTTACCCCGCTGACGCTGGTCAATCAACTGGCGTTAATCGTGCTGCTCGCCACGATTATTGGCGT
CGCCGGCATGGCGATCTCCGCGCGCCTGGTGAACGGCGTGCAGGGCAGCGCGCACGCCATTAACAAGGCCGGTTCATTGC
GTATGCAAAGCTACCGGCTGCTGGCGGCGATCCCGCTGAATGAAAACGATCAAAAACTGGTGGCGGACATGACCGCCACC
GTCTTCAGCCCGGAGCTGCAAAATTCCGCCCGCCGCGACGGCCAGGAAATCCAGCTTAAAGCCCTGCAGCAGTACTGGCA
GCTGGCGCTGGCCCCCGGGATGCAGCGCGCAGTCAATCAGGCGGAAGTGGCCCAGGATGTCGCCGACTTTGTCGATCGTA
TCGACCAGTTGGTCACCGCATTCGACCACACCACCGAGCAGCGTATCGAACGGGTGGTCTGGATCTATCGCATCCTCGCC
ATTGGTATGGCGCTGCTGCTGATCTTCACTATCATCTGGCTACGCGCCCGCCTGCTGCGGCCGTGGAAACAGCTGCTGAG
CATGGCGCGCGCGGTGAGCCAGCGTGATTTCACCCAGCGGGCGCACATCAGCGGCCGCAACGAAATGGCGACCCTGGGCA
TGGCGTTGAATAACATGTCGGAAGAGCTGGCGGAAAGCTATGCCGTGCTCGAGCGGCGGGTGCAGGAGAAGACTGCCGGA
CTGGAGCAGAAGAACGAGATCCTCGCTTTCTTATGGCAGGCCAACCGCCGGCTGCACTCCAGCGCCCCGCTCTGCGAACG
TATTTCGCCGGTACTGAATGGCCTGCAGGGGCTGACGCTGCTGCGCGATATCGAGGTACGGGTTTACGACCTGGAAGACG
AAGATAATCACCAGGAGTTTACCTGCCACTCGGATGATGACTGTGATGATAAAGGCTGCTACCTCTGTCCACGCAATCTG
CCGCCGCTGCCGGATGGCGGAACAACGCTGAAGTGGCGGCTGTCGGATGCGCATAGTCAGTACGGCATCCTGTTGGCGAC
GCTGCCGGTTGGCCGCCATCTCAGCCACGATCAGCAGCAGCTGGTGGATACCCTGGTGGAACAGCTGACCAGCACCCTGG
CGCTGGACCGCCATCAGGAGAAGCAGCAGCAGCTGATCGTCATGGAAGAACGGGCCACTATCGCCCGCGAGCTGCACGAC
TCCATCGCCCAGTCGCTATCCTGTATGAAGATGCAGGTTAGCTGTCTGCAGATGCAAGGCGACGCCCTGCCGACGGAGAG
CCGCCAGCTGCTGGGGCAGATCCGTAACGAACTGAATACCTCGTGGGCCCAGCTGCGGGAGCTGTTAACCACCTTCCGCC
TGCAGTTGACCGAGCCGGGGCTGCGCCCGGCGCTGGAGGCCAGCTGTCAGGAGTACAGCGCCCACTTCGGCTTTACGGTG
CAGCTTGATTACCAGCTCCCGCCGCGCTTTGTGCCCTCGCATCAGGCGATCCACCTGCTGCAGATCGCTCGCGAAGCGTT
GAGCAACGCTCTGAAGCATGCCAGCGCGACCGAAGTGACCGTGACGGTGTCACAACGCGATAATCAGGTTCGTCTGGTGG
TAGCGGATAACGGCCGCGGCGTACCGGACCACGCCGAACGGAGTAACCATTACGGTTTAATTATTATGCGGGATCGCGCC
CAGAGCTTGCGCGGTGACTGTCAGGTACGACGCCGGGAAACCGGTGGAACCGAAGTGATCGTCACGTTTATCCCGGAAAA
AACGTTTTCCATCCAATAA

Upstream 100 bases:

>100_bases
AATTAAGGAGTAACTCTTTGGGGGTATGGGTATACTCCAGGGGATAGTCGAGAATAAGCGCCACTCCCGGAGACATGACC
GGTCAGGAGCCACGCATTCC

Downstream 100 bases:

>100_bases
GGAGACCCTCATGAGTCAACAGGAACGGGCAACTATCCTTCTCATTGACGACCATCCGATGCTGCGCACCGGCGTGAAAC
AGTTGATCAGCATGGCGCCG

Product: nitrate/nitrite sensor protein NarX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MLKRLFTPLTLVNQLALIVLLATIIGVAGMAISARLVNGVQGSAHAINKAGSLRMQSYRLLAAIPLNENDQKLVADMTAT
VFSPELQNSARRDGQEIQLKALQQYWQLALAPGMQRAVNQAEVAQDVADFVDRIDQLVTAFDHTTEQRIERVVWIYRILA
IGMALLLIFTIIWLRARLLRPWKQLLSMARAVSQRDFTQRAHISGRNEMATLGMALNNMSEELAESYAVLERRVQEKTAG
LEQKNEILAFLWQANRRLHSSAPLCERISPVLNGLQGLTLLRDIEVRVYDLEDEDNHQEFTCHSDDDCDDKGCYLCPRNL
PPLPDGGTTLKWRLSDAHSQYGILLATLPVGRHLSHDQQQLVDTLVEQLTSTLALDRHQEKQQQLIVMEERATIARELHD
SIAQSLSCMKMQVSCLQMQGDALPTESRQLLGQIRNELNTSWAQLRELLTTFRLQLTEPGLRPALEASCQEYSAHFGFTV
QLDYQLPPRFVPSHQAIHLLQIAREALSNALKHASATEVTVTVSQRDNQVRLVVADNGRGVPDHAERSNHYGLIIMRDRA
QSLRGDCQVRRRETGGTEVIVTFIPEKTFSIQ

Sequences:

>Translated_592_residues
MLKRLFTPLTLVNQLALIVLLATIIGVAGMAISARLVNGVQGSAHAINKAGSLRMQSYRLLAAIPLNENDQKLVADMTAT
VFSPELQNSARRDGQEIQLKALQQYWQLALAPGMQRAVNQAEVAQDVADFVDRIDQLVTAFDHTTEQRIERVVWIYRILA
IGMALLLIFTIIWLRARLLRPWKQLLSMARAVSQRDFTQRAHISGRNEMATLGMALNNMSEELAESYAVLERRVQEKTAG
LEQKNEILAFLWQANRRLHSSAPLCERISPVLNGLQGLTLLRDIEVRVYDLEDEDNHQEFTCHSDDDCDDKGCYLCPRNL
PPLPDGGTTLKWRLSDAHSQYGILLATLPVGRHLSHDQQQLVDTLVEQLTSTLALDRHQEKQQQLIVMEERATIARELHD
SIAQSLSCMKMQVSCLQMQGDALPTESRQLLGQIRNELNTSWAQLRELLTTFRLQLTEPGLRPALEASCQEYSAHFGFTV
QLDYQLPPRFVPSHQAIHLLQIAREALSNALKHASATEVTVTVSQRDNQVRLVVADNGRGVPDHAERSNHYGLIIMRDRA
QSLRGDCQVRRRETGGTEVIVTFIPEKTFSIQ
>Mature_592_residues
MLKRLFTPLTLVNQLALIVLLATIIGVAGMAISARLVNGVQGSAHAINKAGSLRMQSYRLLAAIPLNENDQKLVADMTAT
VFSPELQNSARRDGQEIQLKALQQYWQLALAPGMQRAVNQAEVAQDVADFVDRIDQLVTAFDHTTEQRIERVVWIYRILA
IGMALLLIFTIIWLRARLLRPWKQLLSMARAVSQRDFTQRAHISGRNEMATLGMALNNMSEELAESYAVLERRVQEKTAG
LEQKNEILAFLWQANRRLHSSAPLCERISPVLNGLQGLTLLRDIEVRVYDLEDEDNHQEFTCHSDDDCDDKGCYLCPRNL
PPLPDGGTTLKWRLSDAHSQYGILLATLPVGRHLSHDQQQLVDTLVEQLTSTLALDRHQEKQQQLIVMEERATIARELHD
SIAQSLSCMKMQVSCLQMQGDALPTESRQLLGQIRNELNTSWAQLRELLTTFRLQLTEPGLRPALEASCQEYSAHFGFTV
QLDYQLPPRFVPSHQAIHLLQIAREALSNALKHASATEVTVTVSQRDNQVRLVVADNGRGVPDHAERSNHYGLIIMRDRA
QSLRGDCQVRRRETGGTEVIVTFIPEKTFSIQ

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate availability to the narL protein and of both nitrate/nitrite to the narP protein. NarX probably activates narL and narP by phosphorylation in the presence of nitrate. NarX also plays a

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=590, Percent_Identity=78.3050847457627, Blast_Score=958, Evalue=0.0,
Organism=Escherichia coli, GI1788812, Length=594, Percent_Identity=29.4612794612795, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI87082311, Length=218, Percent_Identity=29.3577981651376, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR003661
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 66726; Mature: 66726

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRLFTPLTLVNQLALIVLLATIIGVAGMAISARLVNGVQGSAHAINKAGSLRMQSYRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHCEEE
LAAIPLNENDQKLVADMTATVFSPELQNSARRDGQEIQLKALQQYWQLALAPGMQRAVNQ
EEEECCCCCCHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
AEVAQDVADFVDRIDQLVTAFDHTTEQRIERVVWIYRILAIGMALLLIFTIIWLRARLLR
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PWKQLLSMARAVSQRDFTQRAHISGRNEMATLGMALNNMSEELAESYAVLERRVQEKTAG
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LEQKNEILAFLWQANRRLHSSAPLCERISPVLNGLQGLTLLRDIEVRVYDLEDEDNHQEF
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECEEEEEECCCCCCCCCE
TCHSDDDCDDKGCYLCPRNLPPLPDGGTTLKWRLSDAHSQYGILLATLPVGRHLSHDQQQ
ECCCCCCCCCCCCEECCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCHHHH
LVDTLVEQLTSTLALDRHQEKQQQLIVMEERATIARELHDSIAQSLSCMKMQVSCLQMQG
HHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DALPTESRQLLGQIRNELNTSWAQLRELLTTFRLQLTEPGLRPALEASCQEYSAHFGFTV
CCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEE
QLDYQLPPRFVPSHQAIHLLQIAREALSNALKHASATEVTVTVSQRDNQVRLVVADNGRG
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCC
VPDHAERSNHYGLIIMRDRAQSLRGDCQVRRRETGGTEVIVTFIPEKTFSIQ
CCCHHHCCCCEEEEEECCCHHHHHCHHHHHHCCCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MLKRLFTPLTLVNQLALIVLLATIIGVAGMAISARLVNGVQGSAHAINKAGSLRMQSYRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHCEEE
LAAIPLNENDQKLVADMTATVFSPELQNSARRDGQEIQLKALQQYWQLALAPGMQRAVNQ
EEEECCCCCCHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
AEVAQDVADFVDRIDQLVTAFDHTTEQRIERVVWIYRILAIGMALLLIFTIIWLRARLLR
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PWKQLLSMARAVSQRDFTQRAHISGRNEMATLGMALNNMSEELAESYAVLERRVQEKTAG
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LEQKNEILAFLWQANRRLHSSAPLCERISPVLNGLQGLTLLRDIEVRVYDLEDEDNHQEF
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECEEEEEECCCCCCCCCE
TCHSDDDCDDKGCYLCPRNLPPLPDGGTTLKWRLSDAHSQYGILLATLPVGRHLSHDQQQ
ECCCCCCCCCCCCEECCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCHHHH
LVDTLVEQLTSTLALDRHQEKQQQLIVMEERATIARELHDSIAQSLSCMKMQVSCLQMQG
HHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DALPTESRQLLGQIRNELNTSWAQLRELLTTFRLQLTEPGLRPALEASCQEYSAHFGFTV
CCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEE
QLDYQLPPRFVPSHQAIHLLQIAREALSNALKHASATEVTVTVSQRDNQVRLVVADNGRG
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCC
VPDHAERSNHYGLIIMRDRAQSLRGDCQVRRRETGGTEVIVTFIPEKTFSIQ
CCCHHHCCCCEEEEEECCCHHHHHCHHHHHHCCCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]