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The map label for this gene is rnt

Identifier: 152970543

GI number: 152970543

Start: 2185316

End: 2185963

Strand: Direct

Name: rnt

Synonym: KPN_01991

Alternate gene names: 152970543

Gene position: 2185316-2185963 (Clockwise)

Preceding gene: 152970542

Following gene: 152970545

Centisome position: 41.12

GC content: 59.72

Gene sequence:

>648_bases
ATGTCCGAGAACGCTCAACTGAATGGTCTGTGCGACCGTTTCCGCGGTTTTTATCCGGTGGTGATCGATGTTGAAACCGC
AGGATTTAACGCCAAAACCGACGCCCTGCTGGAGATCGCCGCCATTACGTTGAAGATGGATGAGCACGGCTGGCTGATGC
CCGATGAAACGCTGCATTTCCATGTTGAACCTTTCGAGGGCGCCAATCTGCAGCCCGAAGCGCTGGCTTTTAACGGGATT
AACCCGCATGACCCGCAGCGCGGGGCCGTCAGCGAATACGATGCCCTGCATGCCATCTTCAAAATGGTGCGCAAAGGGAT
GAAAGAGAGCGACTGCAGCCGGGCAATCATGGTGGCGCACAATGCCACCTTTGACCACAGCTTCACCATGACCGCCGCCG
AACGCGCGGGACTTAAACGCAACCCTTTCCATCCGTTTGTTACCTTCGACACCGCCGCGCTAAGCGGGCTGGCGCTGGGC
CAGACGGTGCTGTCGAAAGCCTGTATTGCCGCGGGTATGCCGTTCGACGGCGCGCAGGCTCACTCCGCCCTGTATGATAC
CGAGCAGACGGCGCAGCTGTTTTGCGAGATCGTGAACCGCTGGAAGCGTCTGGGCGGCTGGCCGCTGCCCGTAGCCACCC
CGGAGTGA

Upstream 100 bases:

>100_bases
CCCGGGCGCTACGGCGCCCGTTGTTTTCACCCCTGCCTGCTGCAAGCCGCGGCAAAATTTGCCATAATGCGCGCTACTTT
TTTCCAGCTAAGAGAATCTG

Downstream 100 bases:

>100_bases
TAAAAAAAGCGACTTCCAGGAAGTCGCTTTTTTACTGATGGCGGGAATTACTCCGCTTTTGGCTCGTCGGTATGGTACTT
TGCCGCCGTCTCTTTGATCA

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MSENAQLNGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITLKMDEHGWLMPDETLHFHVEPFEGANLQPEALAFNGI
NPHDPQRGAVSEYDALHAIFKMVRKGMKESDCSRAIMVAHNATFDHSFTMTAAERAGLKRNPFHPFVTFDTAALSGLALG
QTVLSKACIAAGMPFDGAQAHSALYDTEQTAQLFCEIVNRWKRLGGWPLPVATPE

Sequences:

>Translated_215_residues
MSENAQLNGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITLKMDEHGWLMPDETLHFHVEPFEGANLQPEALAFNGI
NPHDPQRGAVSEYDALHAIFKMVRKGMKESDCSRAIMVAHNATFDHSFTMTAAERAGLKRNPFHPFVTFDTAALSGLALG
QTVLSKACIAAGMPFDGAQAHSALYDTEQTAQLFCEIVNRWKRLGGWPLPVATPE
>Mature_214_residues
SENAQLNGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITLKMDEHGWLMPDETLHFHVEPFEGANLQPEALAFNGIN
PHDPQRGAVSEYDALHAIFKMVRKGMKESDCSRAIMVAHNATFDHSFTMTAAERAGLKRNPFHPFVTFDTAALSGLALGQ
TVLSKACIAAGMPFDGAQAHSALYDTEQTAQLFCEIVNRWKRLGGWPLPVATPE

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain

Homologues:

Organism=Escherichia coli, GI1787941, Length=212, Percent_Identity=85.8490566037736, Blast_Score=382, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNT_KLEP7 (A6TA01)

Other databases:

- EMBL:   CP000647
- RefSeq:   YP_001335652.1
- ProteinModelPortal:   A6TA01
- SMR:   A6TA01
- STRING:   A6TA01
- GeneID:   5340174
- GenomeReviews:   CP000647_GR
- KEGG:   kpn:KPN_01991
- eggNOG:   COG0847
- HOGENOM:   HBG289562
- OMA:   YDAEKTA
- ProtClustDB:   PRK05168
- GO:   GO:0005622
- HAMAP:   MF_00157
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337
- SMART:   SM00479
- TIGRFAMs:   TIGR01298

Pfam domain/function: PF00929 Exonuc_X-T; SSF53098 RNaseH_fold

EC number: 3.1.13.-

Molecular weight: Translated: 23606; Mature: 23475

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENAQLNGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITLKMDEHGWLMPDETLHF
CCCCCCCHHHHHHHCCCEEEEEEEEECCCCCCHHHHHEEEEEEEEECCCCCCCCCCCEEE
HVEPFEGANLQPEALAFNGINPHDPQRGAVSEYDALHAIFKMVRKGMKESDCSRAIMVAH
EECCCCCCCCCCHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
NATFDHSFTMTAAERAGLKRNPFHPFVTFDTAALSGLALGQTVLSKACIAAGMPFDGAQA
CCCCCCCEEEHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
HSALYDTEQTAQLFCEIVNRWKRLGGWPLPVATPE
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SENAQLNGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITLKMDEHGWLMPDETLHF
CCCCCCHHHHHHHCCCEEEEEEEEECCCCCCHHHHHEEEEEEEEECCCCCCCCCCCEEE
HVEPFEGANLQPEALAFNGINPHDPQRGAVSEYDALHAIFKMVRKGMKESDCSRAIMVAH
EECCCCCCCCCCHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
NATFDHSFTMTAAERAGLKRNPFHPFVTFDTAALSGLALGQTVLSKACIAAGMPFDGAQA
CCCCCCCEEEHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
HSALYDTEQTAQLFCEIVNRWKRLGGWPLPVATPE
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA