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The map label for this gene is rihA [H]

Identifier: 152970464

GI number: 152970464

Start: 2108264

End: 2109199

Strand: Direct

Name: rihA [H]

Synonym: KPN_01912

Alternate gene names: 152970464

Gene position: 2108264-2109199 (Clockwise)

Preceding gene: 152970462

Following gene: 152970467

Centisome position: 39.67

GC content: 62.61

Gene sequence:

>936_bases
ATGGCACTGCCGATTATGATTGATTGCGATCCGGGACATGATGACGCGATCGCCCTGGTCCTCGCCCTCGCCTCGCCGGA
GCTGGAGGTGAAAGCCGTCACCGCTTCCGCGGGCAACCAGACCCCGGAGAAGACCCTGCGCAACGTGCTGCGTATGTTGA
CGCTGCTGAACCGGCCGGACATTCCGGTTGCCGGCGGCGCGTGGAAGCCGTTGATGCGCGATCTGATCATCGCCGATAAC
GTGCACGGCGAGAGCGGACTGGATGGCCCGAGCCTGCCTGAGCCGGCCTTTGCGCCGCAGAACTGCACCGCCGTCGAGCT
GATGGCTAGCGTGCTGCGGGAAAGCCAGGAGTCGGTGACCCTGGTGGCCACCGGGCCGCAGACTAACGTGGCGTTGCTGC
TCGCCAGCCATCCGGAGCTGCACGCGAAGATCGCCCGCATCGTGATCATGGGGGGCGCCATGGGGCTGGGCAACTGGCAG
CCGGCGGCGGAGTTTAATATCTACGTTGATCCGCAGGCCGCGGAAATGGTCTTTCAGTCGGGGATCCCGGTGGTCATGGC
GGGGCTGGATGTTACGCATCGGGCGCAAATCCTTCCCGCCGATATCGAGCGATTCCGCCAGATTGGCAACCCGGTGTCGA
CCATCGTCGCCGAACTGCTCGACTTCTTTATGGCCTACCACAAAGATGAAAAGTGGGGCTTCGACGGCGCGCCGCTGCAC
GATCCCTGCACCATCGCCTGGCTCCTCAAGCCGGAGATCTTTACCACGATCGAACGCTGGGTTGGCGTAGAAACGGAAGG
CAAATATACCCAGGGGATGACGGTGGTCGATTACTACCATCTGACCGGCAATCGGCCCAATACCACCCTGATGCTGGATG
TGGACCGCGAAGCCTTTGTCGATCTGCTCGCCCAGCGGTTAGCCTTCTACGCCTGA

Upstream 100 bases:

>100_bases
CAGCAATCGTGGTCTCCATCACACTATTCTGCACACCATCTCGTCTGCCCCGACTCGCAGCGTTAGTCTGGTCACAGAAC
ATCGCCAACAAGGAGCTGCT

Downstream 100 bases:

>100_bases
CCTCTATGCCAGGCCCCTTTACCCTGGCGGAAAAAGGGGCCTGTTCAGTCGAAGCCCGCCAGGCTAGTCGTCCGCCGCGA
CGCGGATCACTACCTTACCG

Product: ribonucleoside hydrolase 1

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MALPIMIDCDPGHDDAIALVLALASPELEVKAVTASAGNQTPEKTLRNVLRMLTLLNRPDIPVAGGAWKPLMRDLIIADN
VHGESGLDGPSLPEPAFAPQNCTAVELMASVLRESQESVTLVATGPQTNVALLLASHPELHAKIARIVIMGGAMGLGNWQ
PAAEFNIYVDPQAAEMVFQSGIPVVMAGLDVTHRAQILPADIERFRQIGNPVSTIVAELLDFFMAYHKDEKWGFDGAPLH
DPCTIAWLLKPEIFTTIERWVGVETEGKYTQGMTVVDYYHLTGNRPNTTLMLDVDREAFVDLLAQRLAFYA

Sequences:

>Translated_311_residues
MALPIMIDCDPGHDDAIALVLALASPELEVKAVTASAGNQTPEKTLRNVLRMLTLLNRPDIPVAGGAWKPLMRDLIIADN
VHGESGLDGPSLPEPAFAPQNCTAVELMASVLRESQESVTLVATGPQTNVALLLASHPELHAKIARIVIMGGAMGLGNWQ
PAAEFNIYVDPQAAEMVFQSGIPVVMAGLDVTHRAQILPADIERFRQIGNPVSTIVAELLDFFMAYHKDEKWGFDGAPLH
DPCTIAWLLKPEIFTTIERWVGVETEGKYTQGMTVVDYYHLTGNRPNTTLMLDVDREAFVDLLAQRLAFYA
>Mature_310_residues
ALPIMIDCDPGHDDAIALVLALASPELEVKAVTASAGNQTPEKTLRNVLRMLTLLNRPDIPVAGGAWKPLMRDLIIADNV
HGESGLDGPSLPEPAFAPQNCTAVELMASVLRESQESVTLVATGPQTNVALLLASHPELHAKIARIVIMGGAMGLGNWQP
AAEFNIYVDPQAAEMVFQSGIPVVMAGLDVTHRAQILPADIERFRQIGNPVSTIVAELLDFFMAYHKDEKWGFDGAPLHD
PCTIAWLLKPEIFTTIERWVGVETEGKYTQGMTVVDYYHLTGNRPNTTLMLDVDREAFVDLLAQRLAFYA

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=311, Percent_Identity=85.8520900321543, Blast_Score=564, Evalue=1e-162,
Organism=Escherichia coli, GI1788486, Length=306, Percent_Identity=38.562091503268, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1786213, Length=309, Percent_Identity=42.3948220064725, Blast_Score=210, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI32565474, Length=189, Percent_Identity=31.7460317460317, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17565698, Length=188, Percent_Identity=34.0425531914894, Blast_Score=88, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6320608, Length=259, Percent_Identity=35.1351351351351, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24641839, Length=310, Percent_Identity=28.7096774193548, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24583915, Length=333, Percent_Identity=25.5255255255255, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24641837, Length=319, Percent_Identity=27.5862068965517, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 33902; Mature: 33771

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALPIMIDCDPGHDDAIALVLALASPELEVKAVTASAGNQTPEKTLRNVLRMLTLLNRPD
CCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCC
IPVAGGAWKPLMRDLIIADNVHGESGLDGPSLPEPAFAPQNCTAVELMASVLRESQESVT
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEE
LVATGPQTNVALLLASHPELHAKIARIVIMGGAMGLGNWQPAAEFNIYVDPQAAEMVFQS
EEEECCCCCEEEEEECCCHHHHHHHHHEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHC
GIPVVMAGLDVTHRAQILPADIERFRQIGNPVSTIVAELLDFFMAYHKDEKWGFDGAPLH
CCCEEEECCCHHHHCEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
DPCTIAWLLKPEIFTTIERWVGVETEGKYTQGMTVVDYYHLTGNRPNTTLMLDVDREAFV
CCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCEEEEEECCHHHHH
DLLAQRLAFYA
HHHHHHHHHCC
>Mature Secondary Structure 
ALPIMIDCDPGHDDAIALVLALASPELEVKAVTASAGNQTPEKTLRNVLRMLTLLNRPD
CCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCC
IPVAGGAWKPLMRDLIIADNVHGESGLDGPSLPEPAFAPQNCTAVELMASVLRESQESVT
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEE
LVATGPQTNVALLLASHPELHAKIARIVIMGGAMGLGNWQPAAEFNIYVDPQAAEMVFQS
EEEECCCCCEEEEEECCCHHHHHHHHHEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHC
GIPVVMAGLDVTHRAQILPADIERFRQIGNPVSTIVAELLDFFMAYHKDEKWGFDGAPLH
CCCEEEECCCHHHHCEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
DPCTIAWLLKPEIFTTIERWVGVETEGKYTQGMTVVDYYHLTGNRPNTTLMLDVDREAFV
CCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCEEEEEECCHHHHH
DLLAQRLAFYA
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA