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The map label for this gene is pcaR [H]

Identifier: 152970428

GI number: 152970428

Start: 2066165

End: 2067055

Strand: Direct

Name: pcaR [H]

Synonym: KPN_01876

Alternate gene names: 152970428

Gene position: 2066165-2067055 (Clockwise)

Preceding gene: 152970420

Following gene: 152970430

Centisome position: 38.87

GC content: 63.19

Gene sequence:

>891_bases
ATGGTGGTAAAACCGGCACAATTCAGCGCGCCCCATTTTGCGCAAGGTATAGTGATCTGCCGTGAGCTTTACGCTGCAAC
GGAGAAGGATGCGATGAGCGACAATATACCCAATACGCTGAGCGCCAGGCAGGCCGAGCACGACCCTAACTTTATGCTGT
CTCTGGCCCGCGGGCTTGAGGTCCTTAACGCCTTCACCCCCCAGCGCCAGCGGCTGACCATTTCCCAGCTCAGCCAGAAA
ACGCAAATCTCGCGCGCGGCGGTACGCCGCTGTTTGTATACCCTCGCCGCGCTGGGCATGGTGCACAGTCCGGATGGCCG
TAGCTATGAACTGCTCCCCCGGGTGCTGGCCGTCGGCCACGCCTATCTGGCAGGCACGCCGCTGGCCAAGGTGGCGCAGA
CGGCGCTGGATAATCTTGGCAAAGCGCTCGGCGAATCCTGTTCGGCGGCCACGCTCGACGGGGATAACGTGCTCTATATT
GCCCGCGCGGCGGTCAATAATTTACTGAGCATCGATCTGGGGCGAGGCAGCCGCCTGCCGGCGTGGGCCACGTCGATGGG
GCGGGTGTTGCTGAGCGCACTGCCGGAGGAACAGCTGGAAGTGACGTTGTCACGGGCTACGCTTATTCGCTACACTCCGC
ATACCCTGTGCGATTTGTCCGGTTTACGTGCAGAGATCGCCCGGGTGCGCATGCAGGGCTATGCCCTCGCCGACCGGCAG
ATCGAGGTAGGCTTGTGCTCGCTGGCGGTACCCGTGCTGTCGCGTCACGGTCAGGTGGTGGCCGCCCTGAATGTCGGGGT
ACCTGCCGCGACGGTCAGCGCCGCGGCGTTAAAAGAGAAGGCTCTCGCCCCGCTACGGCGCGCGGCGATGGATCTGTCGC
TGCAGCTATAG

Upstream 100 bases:

>100_bases
CATTGACAATAGGGTTCAGCCTGGCGGATGGTCAACGGCGATAATCGAAATGTTGTGCGATTATCGACGCGTTTGTTAAA
AGACGGTTATTAATGTGATC

Downstream 100 bases:

>100_bases
CGCCGACTGCGGTTTCGCCACGGTGGCGAGGCTGCGCCAGATCCCGGCGGCCAGCAGCAGAAGCAATACACCCGCCGTCG
CCAGCACCAGGCTATGAGCC

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MVVKPAQFSAPHFAQGIVICRELYAATEKDAMSDNIPNTLSARQAEHDPNFMLSLARGLEVLNAFTPQRQRLTISQLSQK
TQISRAAVRRCLYTLAALGMVHSPDGRSYELLPRVLAVGHAYLAGTPLAKVAQTALDNLGKALGESCSAATLDGDNVLYI
ARAAVNNLLSIDLGRGSRLPAWATSMGRVLLSALPEEQLEVTLSRATLIRYTPHTLCDLSGLRAEIARVRMQGYALADRQ
IEVGLCSLAVPVLSRHGQVVAALNVGVPAATVSAAALKEKALAPLRRAAMDLSLQL

Sequences:

>Translated_296_residues
MVVKPAQFSAPHFAQGIVICRELYAATEKDAMSDNIPNTLSARQAEHDPNFMLSLARGLEVLNAFTPQRQRLTISQLSQK
TQISRAAVRRCLYTLAALGMVHSPDGRSYELLPRVLAVGHAYLAGTPLAKVAQTALDNLGKALGESCSAATLDGDNVLYI
ARAAVNNLLSIDLGRGSRLPAWATSMGRVLLSALPEEQLEVTLSRATLIRYTPHTLCDLSGLRAEIARVRMQGYALADRQ
IEVGLCSLAVPVLSRHGQVVAALNVGVPAATVSAAALKEKALAPLRRAAMDLSLQL
>Mature_296_residues
MVVKPAQFSAPHFAQGIVICRELYAATEKDAMSDNIPNTLSARQAEHDPNFMLSLARGLEVLNAFTPQRQRLTISQLSQK
TQISRAAVRRCLYTLAALGMVHSPDGRSYELLPRVLAVGHAYLAGTPLAKVAQTALDNLGKALGESCSAATLDGDNVLYI
ARAAVNNLLSIDLGRGSRLPAWATSMGRVLLSALPEEQLEVTLSRATLIRYTPHTLCDLSGLRAEIARVRMQGYALADRQ
IEVGLCSLAVPVLSRHGQVVAALNVGVPAATVSAAALKEKALAPLRRAAMDLSLQL

Specific function: Positive regulator of all genes within the pca regulon, pcaBDC, pcaIJ and pcaF. Also required for the chemotactic response to aromatic compounds [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=226, Percent_Identity=30.5309734513274, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788131, Length=252, Percent_Identity=28.1746031746032, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87082359, Length=244, Percent_Identity=25.8196721311475, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1789999, Length=257, Percent_Identity=25.2918287937743, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1786715, Length=221, Percent_Identity=25.7918552036199, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 31667; Mature: 31667

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVKPAQFSAPHFAQGIVICRELYAATEKDAMSDNIPNTLSARQAEHDPNFMLSLARGLE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHH
VLNAFTPQRQRLTISQLSQKTQISRAAVRRCLYTLAALGMVHSPDGRSYELLPRVLAVGH
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
AYLAGTPLAKVAQTALDNLGKALGESCSAATLDGDNVLYIARAAVNNLLSIDLGRGSRLP
HHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEHHHHHHHHEEECCCCCCCC
AWATSMGRVLLSALPEEQLEVTLSRATLIRYTPHTLCDLSGLRAEIARVRMQGYALADRQ
HHHHHHHHHHHHHCCHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCC
IEVGLCSLAVPVLSRHGQVVAALNVGVPAATVSAAALKEKALAPLRRAAMDLSLQL
HHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVVKPAQFSAPHFAQGIVICRELYAATEKDAMSDNIPNTLSARQAEHDPNFMLSLARGLE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHH
VLNAFTPQRQRLTISQLSQKTQISRAAVRRCLYTLAALGMVHSPDGRSYELLPRVLAVGH
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
AYLAGTPLAKVAQTALDNLGKALGESCSAATLDGDNVLYIARAAVNNLLSIDLGRGSRLP
HHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEHHHHHHHHEEECCCCCCCC
AWATSMGRVLLSALPEEQLEVTLSRATLIRYTPHTLCDLSGLRAEIARVRMQGYALADRQ
HHHHHHHHHHHHHCCHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCC
IEVGLCSLAVPVLSRHGQVVAALNVGVPAATVSAAALKEKALAPLRRAAMDLSLQL
HHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8083169 [H]