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The map label for this gene is gsiB [H]

Identifier: 152970406

GI number: 152970406

Start: 2043490

End: 2045112

Strand: Direct

Name: gsiB [H]

Synonym: KPN_01854

Alternate gene names: 152970406

Gene position: 2043490-2045112 (Clockwise)

Preceding gene: 152970405

Following gene: 152970407

Centisome position: 38.45

GC content: 60.75

Gene sequence:

>1623_bases
ATGCATCGTCATTTTCGCCTGCCCGCGCTGGCCGCGCTGTTTCTCACCGGCGCCTTTTCCGTCTGGGCCGCCGACACGCC
GGTTAAAGGCGGCACCTTAATTTATCTGGAGCAGCAACCGCACACCAATCTCTATCCGCCCGCCGGCGGCTTTTACCCGA
ATGGCGGGATTCTCAATCAGATAACCGACAAGCTCACCTGGCAGAACCCGAAAACGCTGGAGATTGAGCCGTGGATCGCC
GAGAGCTGGACCAGCAATGCCGATAAAACCGAGTACACTTTCCACCTGCGCAAAGGGGTCACTTTCTCAGATGGCACACC
GCTGGACGCCGCCGCCGTCGCCAAAAACTTCGATACCTATGGTCTGGGAGATAAAGCCCATCGTCTGCCCGTCTCGGAGG
TCATCAATAACTACCAGCGCAGCGAAGTGATCGACCCGCTGACGGTAAAATTCTACTTCAACAAGCCGTCGCCGGGCTTC
CTGCAGGGAACCGCCACCATCGGCTCCGGTCTGGTATCTCTCAGCACCCTGCAGCGTAATTTTGAAGAGCTGGGCGATGC
CCGACATATTATCGGCTCCGGCCCCTTCGTCGTGCAGGATGAAAAACCAGGCCGCGAGCTGACGCTGGTGGCGCGCAAGG
ATTACCAGTGGGGGCCGAAAAACATTGCCCAGCAGGGGCCCGCTAACCTCGACGGCATCACCTATATTGTGACGCCGGAA
GACAGCGTGCGTATCGGCGCGCTGCTGGCAGGTCAGGCGGGCTTTATTCGCCAGGTGCAGGCCTACGATGAAAAGCAGGC
TACCGATCAGGGATTTAAGATCTACGCTGCCCCTACCCGCGGGGTCAACGACAGCCTGAGCTTCCGCCCGGATAACCCAC
TGGTCGCCGACCTGCGGGTGCGTCAGGCCCTGCTCCATGCCACCAACGCCAGGCAGGTGGTGGAGACGCTGTTCTCCGCT
AACTACCCGCAAGCCACCTCCGTGCTGGCCAGCTCTGCCGCCGGCTATGTCAATCTCAGCGATAAACTCACCTTCGACCA
GGCCAAAGCCCGGCAGTTGCTGGATGACGCCGGCTGGAAGCCCGCCGCCGACGGTATTCGCAGCAAAGACGGTCAGCGGC
TGGCGCTGACCGTTTATGAGTCGCTGCCGCAGCCGCAAAATAAGGAAGTGCTGCAGCTGATCGCCCAGCAGTGGCGCCAG
GTGGGTGTGGCGTTGACGGTCAAAGCCGGGGATGCCGGTAGCCGTACGCTGGATAACCTCGACCCGCAGAAAACCCCGCT
CACCGTCTCAGAAGTGGGCCGCGCCGACCCGGATGTGGTGAAAAGTATGTTCTTCCCCAACAACCGCGATGCGCTGCTGC
AGAAAGGCGGCTCCAGCGATAAAGTGCAGCGCTTCCGCGATGATAAGCTCAATGACCTGCTGACCGGCATCTCCGCGGCG
GTTGAACCGCAGCAGCGCTTGCAGTTAACCGGTGACGCCCAGCGCTATCTGATCGATAACGCCTATGTGATCCCTATTTT
CGAAGAGCCGCAGGTGTTCGCCGGCGCGCCGTGGGTCAAAGGCGTCAGCTTCGAGGCCGTCGGTCGTCCGTCGTTCTACG
GCGCGTGGCTGGACAAGCACTAA

Upstream 100 bases:

>100_bases
GTAAATGCAATTTCCGCAAATGCTTAGCGCAATTCCTTGGTTCCTTTGTTTTTAGCCGCGCCCGATGATGGCAACACAAT
AGATAACAAAGGAATTCACC

Downstream 100 bases:

>100_bases
GAGGCCGGTTATGAGCGCTTATCTTCTGCGACGATTCGGCCAGGGGCTGCTGGTCCTGTGGGCGGCCTTTACCCTCACCT
TTTTCCTGCTGCAGGTCCTG

Product: nickel ABC transporter periplasmic substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MHRHFRLPALAALFLTGAFSVWAADTPVKGGTLIYLEQQPHTNLYPPAGGFYPNGGILNQITDKLTWQNPKTLEIEPWIA
ESWTSNADKTEYTFHLRKGVTFSDGTPLDAAAVAKNFDTYGLGDKAHRLPVSEVINNYQRSEVIDPLTVKFYFNKPSPGF
LQGTATIGSGLVSLSTLQRNFEELGDARHIIGSGPFVVQDEKPGRELTLVARKDYQWGPKNIAQQGPANLDGITYIVTPE
DSVRIGALLAGQAGFIRQVQAYDEKQATDQGFKIYAAPTRGVNDSLSFRPDNPLVADLRVRQALLHATNARQVVETLFSA
NYPQATSVLASSAAGYVNLSDKLTFDQAKARQLLDDAGWKPAADGIRSKDGQRLALTVYESLPQPQNKEVLQLIAQQWRQ
VGVALTVKAGDAGSRTLDNLDPQKTPLTVSEVGRADPDVVKSMFFPNNRDALLQKGGSSDKVQRFRDDKLNDLLTGISAA
VEPQQRLQLTGDAQRYLIDNAYVIPIFEEPQVFAGAPWVKGVSFEAVGRPSFYGAWLDKH

Sequences:

>Translated_540_residues
MHRHFRLPALAALFLTGAFSVWAADTPVKGGTLIYLEQQPHTNLYPPAGGFYPNGGILNQITDKLTWQNPKTLEIEPWIA
ESWTSNADKTEYTFHLRKGVTFSDGTPLDAAAVAKNFDTYGLGDKAHRLPVSEVINNYQRSEVIDPLTVKFYFNKPSPGF
LQGTATIGSGLVSLSTLQRNFEELGDARHIIGSGPFVVQDEKPGRELTLVARKDYQWGPKNIAQQGPANLDGITYIVTPE
DSVRIGALLAGQAGFIRQVQAYDEKQATDQGFKIYAAPTRGVNDSLSFRPDNPLVADLRVRQALLHATNARQVVETLFSA
NYPQATSVLASSAAGYVNLSDKLTFDQAKARQLLDDAGWKPAADGIRSKDGQRLALTVYESLPQPQNKEVLQLIAQQWRQ
VGVALTVKAGDAGSRTLDNLDPQKTPLTVSEVGRADPDVVKSMFFPNNRDALLQKGGSSDKVQRFRDDKLNDLLTGISAA
VEPQQRLQLTGDAQRYLIDNAYVIPIFEEPQVFAGAPWVKGVSFEAVGRPSFYGAWLDKH
>Mature_540_residues
MHRHFRLPALAALFLTGAFSVWAADTPVKGGTLIYLEQQPHTNLYPPAGGFYPNGGILNQITDKLTWQNPKTLEIEPWIA
ESWTSNADKTEYTFHLRKGVTFSDGTPLDAAAVAKNFDTYGLGDKAHRLPVSEVINNYQRSEVIDPLTVKFYFNKPSPGF
LQGTATIGSGLVSLSTLQRNFEELGDARHIIGSGPFVVQDEKPGRELTLVARKDYQWGPKNIAQQGPANLDGITYIVTPE
DSVRIGALLAGQAGFIRQVQAYDEKQATDQGFKIYAAPTRGVNDSLSFRPDNPLVADLRVRQALLHATNARQVVETLFSA
NYPQATSVLASSAAGYVNLSDKLTFDQAKARQLLDDAGWKPAADGIRSKDGQRLALTVYESLPQPQNKEVLQLIAQQWRQ
VGVALTVKAGDAGSRTLDNLDPQKTPLTVSEVGRADPDVVKSMFFPNNRDALLQKGGSSDKVQRFRDDKLNDLLTGISAA
VEPQQRLQLTGDAQRYLIDNAYVIPIFEEPQVFAGAPWVKGVSFEAVGRPSFYGAWLDKH

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=353, Percent_Identity=29.4617563739377, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789887, Length=444, Percent_Identity=27.027027027027, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789966, Length=496, Percent_Identity=23.1854838709677, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787762, Length=473, Percent_Identity=24.1014799154334, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787551, Length=515, Percent_Identity=22.3300970873786, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59294; Mature: 59294

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRHFRLPALAALFLTGAFSVWAADTPVKGGTLIYLEQQPHTNLYPPAGGFYPNGGILNQ
CCCCCCCHHHHHHHHHHHHHEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHH
ITDKLTWQNPKTLEIEPWIAESWTSNADKTEYTFHLRKGVTFSDGTPLDAAAVAKNFDTY
HHHHHCCCCCCEEEECCCHHHCCCCCCCCCEEEEEEECCCEECCCCCCHHHHHHHCCCCC
GLGDKAHRLPVSEVINNYQRSEVIDPLTVKFYFNKPSPGFLQGTATIGSGLVSLSTLQRN
CCCCHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHH
FEELGDARHIIGSGPFVVQDEKPGRELTLVARKDYQWGPKNIAQQGPANLDGITYIVTPE
HHHHCCHHEECCCCCEEEECCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCEEEEECCC
DSVRIGALLAGQAGFIRQVQAYDEKQATDQGFKIYAAPTRGVNDSLSFRPDNPLVADLRV
CCEEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHH
RQALLHATNARQVVETLFSANYPQATSVLASSAAGYVNLSDKLTFDQAKARQLLDDAGWK
HHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHCCCC
PAADGIRSKDGQRLALTVYESLPQPQNKEVLQLIAQQWRQVGVALTVKAGDAGSRTLDNL
CCCCCCCCCCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCC
DPQKTPLTVSEVGRADPDVVKSMFFPNNRDALLQKGGSSDKVQRFRDDKLNDLLTGISAA
CCCCCCEEHHHHCCCCHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
VEPQQRLQLTGDAQRYLIDNAYVIPIFEEPQVFAGAPWVKGVSFEAVGRPSFYGAWLDKH
CCHHHHEEECCCCCEEEECCEEEEEEECCCCEEECCCCCCCCCEEECCCCCEECCCCCCC
>Mature Secondary Structure
MHRHFRLPALAALFLTGAFSVWAADTPVKGGTLIYLEQQPHTNLYPPAGGFYPNGGILNQ
CCCCCCCHHHHHHHHHHHHHEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHH
ITDKLTWQNPKTLEIEPWIAESWTSNADKTEYTFHLRKGVTFSDGTPLDAAAVAKNFDTY
HHHHHCCCCCCEEEECCCHHHCCCCCCCCCEEEEEEECCCEECCCCCCHHHHHHHCCCCC
GLGDKAHRLPVSEVINNYQRSEVIDPLTVKFYFNKPSPGFLQGTATIGSGLVSLSTLQRN
CCCCHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHH
FEELGDARHIIGSGPFVVQDEKPGRELTLVARKDYQWGPKNIAQQGPANLDGITYIVTPE
HHHHCCHHEECCCCCEEEECCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCEEEEECCC
DSVRIGALLAGQAGFIRQVQAYDEKQATDQGFKIYAAPTRGVNDSLSFRPDNPLVADLRV
CCEEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHH
RQALLHATNARQVVETLFSANYPQATSVLASSAAGYVNLSDKLTFDQAKARQLLDDAGWK
HHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHCCCC
PAADGIRSKDGQRLALTVYESLPQPQNKEVLQLIAQQWRQVGVALTVKAGDAGSRTLDNL
CCCCCCCCCCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCC
DPQKTPLTVSEVGRADPDVVKSMFFPNNRDALLQKGGSSDKVQRFRDDKLNDLLTGISAA
CCCCCCEEHHHHCCCCHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
VEPQQRLQLTGDAQRYLIDNAYVIPIFEEPQVFAGAPWVKGVSFEAVGRPSFYGAWLDKH
CCHHHHEEECCCCCEEEECCEEEEEEECCCCEEECCCCCCCCCEEECCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA