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The map label for this gene is braD [H]

Identifier: 152970169

GI number: 152970169

Start: 1797708

End: 1799282

Strand: Direct

Name: braD [H]

Synonym: KPN_01617

Alternate gene names: 152970169

Gene position: 1797708-1799282 (Clockwise)

Preceding gene: 152970168

Following gene: 152970170

Centisome position: 33.82

GC content: 65.21

Gene sequence:

>1575_bases
ATGAACGCATTGCGTCTCATGAGTGTGTTGGCGCTATTGCTGACCTTGCTGCCATGGCGGGCGCAGGCCGCGGAGGCGGA
CGACTTCGTTGCCGCCAGTCGCAGCCAGCAGGCGCAGCTGTTGACCCAGTGGGCGGCAGCGCCGCAGGCGGATCGTCTGC
CGCTGCTGCGGGCGTTGACCACCGAGAGCCTGGTGATGGACGACGGAAAACATGCTTTCCGCACCCGACAGGGCGGATTA
CAGCCATTGGGCGCTGTCGCCGCGCCGCAGGGCGAAACCCGACCGGTCCGTCTGACCAACAGGCTGCGCAATCTGGCTGC
CGGCGCGCTGGCCAGCCACCTTATTTTAAGTGACAACGTCACAGAGCGAGCTTCCGCCGCACGAACGCTACAGCGGGAAG
CGACCCCGGCCATGGCCGCGCTGCTGCAACAGCGGCTGCAGGCGGAAACCGATGATAATGTGCGGGGTCTGCTGGAGGTG
GCGCTGGCGCGCCTGCAGCTCACTCAGCCTGAGGCCAGCGCGCGGCTGGCCGCCGTGACGCTGCTCGGCCATTCCGCGGA
CCCGGAAACCCAGGCGCTGCTGATCCCCTTTACCGATGCCCAGCATGAGCCGGATGCGGCGGTGCGCGAGGCGGCCAGCG
ATAGCCTGCAAAAGATCAAACATCGTCTGCTGCTCGGCGATCTGCTGGGCCAGGCCTTTATGGGGCTGTCGCTGGGCTCG
GTGCTGCTGCTGGCCGCGCTGGGGCTGGCGATCACCTATGGCCTGCTGGGGGTGATTAACATGGCCCACGGCGAGATGTT
AATGATCGGCGCCTACAGCTGCTGGCTGGTACAGCAGGCGCTGGCGCAGCTCGCGCCGCAATGGCTGGCCTTTTATCCGC
TGGTCGCGCTGCCGGTGGCGTTTCTGGTGACCGCCGGGATCGGCATGGCGCTGGAGCGCACCATTATTCGTCATCTGTAT
GGTCGGCCGCTGGAAACGCTGCTCGCCACCTGGGGGATCAGCCTGATGCTAATCCAGCTGGTGAGGATGCTGTTTGGCGC
GCAGAACGTGGAGGTGGCAAACCCGGCCTGGCTCTCCGGCGGCGTGCAGGTCCTGCCGAACCTGATCCTGCCGTGGAACC
GGCTGGCGGTGCTGGCGTTCGTCCTGCTGGTGCTGTGCTTTACCTGGCTCATCCTGAACCGCACCCGTCTGGGGATGAAC
GTCCGCGCCGTGACGCAGAACCGGGCGATGGCCGCCTGCTGCGGCGTACCTACCGGGCGGGTGGATATGCTGGCCTTTGG
CCTCGGCTCCGGTATTGCCGGGCTGGGCGGCGTGGCGCTGTCGCAGCTTGGCAACGTCGGGCCTGAACTGGGTCAGGGAT
ACATCATCGACTCGTTTCTGGTGGTGGTGCTGGGCGGCGTCGGCCAGCTGGCCGGCAGCGTGGCGGCGGCCTTTGGGCTG
GGCATTTTCAACAAAATTCTCGAACCGCAAATGGGCGCGGTGCTGGGCAAGATCCTGATCCTGGTGATGATTATTCTGTT
TATTCAGAAACGACCGCAGGGTCTGTTCGCGCTGAAAGGGAGGGTTATCGACTAA

Upstream 100 bases:

>100_bases
AAGAAACCTGACCATCCGGTGAAAACCGTCAGCCAGTAAGCGCCACGGCCGACGGGGCGGGAGCGTCGCGCCCGTCGGCC
GCTGACCAGAGGAGAACGAC

Downstream 100 bases:

>100_bases
TGAGCCAACCAATCACCTTAACGCTGGCGCAGCGCGCGCCGCGGCCTCTGCGCTGGCTGGGGATCCTGCTGGTGCTGGGG
CTGCTGAGTATGCCATTCCT

Product: high-affinity branched-chain amino acid ABC transporter membrane protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MNALRLMSVLALLLTLLPWRAQAAEADDFVAASRSQQAQLLTQWAAAPQADRLPLLRALTTESLVMDDGKHAFRTRQGGL
QPLGAVAAPQGETRPVRLTNRLRNLAAGALASHLILSDNVTERASAARTLQREATPAMAALLQQRLQAETDDNVRGLLEV
ALARLQLTQPEASARLAAVTLLGHSADPETQALLIPFTDAQHEPDAAVREAASDSLQKIKHRLLLGDLLGQAFMGLSLGS
VLLLAALGLAITYGLLGVINMAHGEMLMIGAYSCWLVQQALAQLAPQWLAFYPLVALPVAFLVTAGIGMALERTIIRHLY
GRPLETLLATWGISLMLIQLVRMLFGAQNVEVANPAWLSGGVQVLPNLILPWNRLAVLAFVLLVLCFTWLILNRTRLGMN
VRAVTQNRAMAACCGVPTGRVDMLAFGLGSGIAGLGGVALSQLGNVGPELGQGYIIDSFLVVVLGGVGQLAGSVAAAFGL
GIFNKILEPQMGAVLGKILILVMIILFIQKRPQGLFALKGRVID

Sequences:

>Translated_524_residues
MNALRLMSVLALLLTLLPWRAQAAEADDFVAASRSQQAQLLTQWAAAPQADRLPLLRALTTESLVMDDGKHAFRTRQGGL
QPLGAVAAPQGETRPVRLTNRLRNLAAGALASHLILSDNVTERASAARTLQREATPAMAALLQQRLQAETDDNVRGLLEV
ALARLQLTQPEASARLAAVTLLGHSADPETQALLIPFTDAQHEPDAAVREAASDSLQKIKHRLLLGDLLGQAFMGLSLGS
VLLLAALGLAITYGLLGVINMAHGEMLMIGAYSCWLVQQALAQLAPQWLAFYPLVALPVAFLVTAGIGMALERTIIRHLY
GRPLETLLATWGISLMLIQLVRMLFGAQNVEVANPAWLSGGVQVLPNLILPWNRLAVLAFVLLVLCFTWLILNRTRLGMN
VRAVTQNRAMAACCGVPTGRVDMLAFGLGSGIAGLGGVALSQLGNVGPELGQGYIIDSFLVVVLGGVGQLAGSVAAAFGL
GIFNKILEPQMGAVLGKILILVMIILFIQKRPQGLFALKGRVID
>Mature_524_residues
MNALRLMSVLALLLTLLPWRAQAAEADDFVAASRSQQAQLLTQWAAAPQADRLPLLRALTTESLVMDDGKHAFRTRQGGL
QPLGAVAAPQGETRPVRLTNRLRNLAAGALASHLILSDNVTERASAARTLQREATPAMAALLQQRLQAETDDNVRGLLEV
ALARLQLTQPEASARLAAVTLLGHSADPETQALLIPFTDAQHEPDAAVREAASDSLQKIKHRLLLGDLLGQAFMGLSLGS
VLLLAALGLAITYGLLGVINMAHGEMLMIGAYSCWLVQQALAQLAPQWLAFYPLVALPVAFLVTAGIGMALERTIIRHLY
GRPLETLLATWGISLMLIQLVRMLFGAQNVEVANPAWLSGGVQVLPNLILPWNRLAVLAFVLLVLCFTWLILNRTRLGMN
VRAVTQNRAMAACCGVPTGRVDMLAFGLGSGIAGLGGVALSQLGNVGPELGQGYIIDSFLVVVLGGVGQLAGSVAAAFGL
GIFNKILEPQMGAVLGKILILVMIILFIQKRPQGLFALKGRVID

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=297, Percent_Identity=28.6195286195286, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 55836; Mature: 55836

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALRLMSVLALLLTLLPWRAQAAEADDFVAASRSQQAQLLTQWAAAPQADRLPLLRALT
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHH
TESLVMDDGKHAFRTRQGGLQPLGAVAAPQGETRPVRLTNRLRNLAAGALASHLILSDNV
HHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCH
TERASAARTLQREATPAMAALLQQRLQAETDDNVRGLLEVALARLQLTQPEASARLAAVT
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LLGHSADPETQALLIPFTDAQHEPDAAVREAASDSLQKIKHRLLLGDLLGQAFMGLSLGS
HHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLLLAALGLAITYGLLGVINMAHGEMLMIGAYSCWLVQQALAQLAPQWLAFYPLVALPVA
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLVTAGIGMALERTIIRHLYGRPLETLLATWGISLMLIQLVRMLFGAQNVEVANPAWLSG
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCC
GVQVLPNLILPWNRLAVLAFVLLVLCFTWLILNRTRLGMNVRAVTQNRAMAACCGVPTGR
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHCCHHHHHHCCCCCC
VDMLAFGLGSGIAGLGGVALSQLGNVGPELGQGYIIDSFLVVVLGGVGQLAGSVAAAFGL
HHHHHHHHCCCHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
GIFNKILEPQMGAVLGKILILVMIILFIQKRPQGLFALKGRVID
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
>Mature Secondary Structure
MNALRLMSVLALLLTLLPWRAQAAEADDFVAASRSQQAQLLTQWAAAPQADRLPLLRALT
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHH
TESLVMDDGKHAFRTRQGGLQPLGAVAAPQGETRPVRLTNRLRNLAAGALASHLILSDNV
HHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCH
TERASAARTLQREATPAMAALLQQRLQAETDDNVRGLLEVALARLQLTQPEASARLAAVT
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LLGHSADPETQALLIPFTDAQHEPDAAVREAASDSLQKIKHRLLLGDLLGQAFMGLSLGS
HHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLLLAALGLAITYGLLGVINMAHGEMLMIGAYSCWLVQQALAQLAPQWLAFYPLVALPVA
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLVTAGIGMALERTIIRHLYGRPLETLLATWGISLMLIQLVRMLFGAQNVEVANPAWLSG
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCC
GVQVLPNLILPWNRLAVLAFVLLVLCFTWLILNRTRLGMNVRAVTQNRAMAACCGVPTGR
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHCCHHHHHHCCCCCC
VDMLAFGLGSGIAGLGGVALSQLGNVGPELGQGYIIDSFLVVVLGGVGQLAGSVAAAFGL
HHHHHHHHCCCHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
GIFNKILEPQMGAVLGKILILVMIILFIQKRPQGLFALKGRVID
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]