Click here to switch to the map view.

The map label for this gene is apbE [H]

Identifier: 152970101

GI number: 152970101

Start: 1724594

End: 1725544

Strand: Direct

Name: apbE [H]

Synonym: KPN_01549

Alternate gene names: 152970101

Gene position: 1724594-1725544 (Clockwise)

Preceding gene: 152970100

Following gene: 152970104

Centisome position: 32.45

GC content: 59.83

Gene sequence:

>951_bases
ATGTCCGATAACCGCGTCTATAGCTACTCCGCCGTGTTGATGGGCTCGCCCATCCTGCTCAAACTCTGTTCCCACGATGA
GGCGATGGCCTCCCGCGTTTTCCAGCTTATCAAGCGCTACGAAGACCTGCTTACCGTTAACCGCGCCGAGTCGCAGGTGA
TGGATATCAACCATGCCGCCGGGCGTCATCCGGTCACCGTCAGCCGACCGGTCTTTCAGCTGATCCAGTGCGCCAAAGCC
GCCAGCATGGTGCGCGACAGCGCCTTTAACCTCGCCATCGGCCCGCTGGTGAAGCTGTGGCGAATTGGTTTTCACGGGCA
CAGCGTGCCTGACGCGGCTGAGATCAGAGCCCGACTGGCGCTGACCCGCCCACAGGAGGTGATCCTTGATGAGGCAACCT
GCAGCGTGTTTCTCCAACAGCCGGGGATGGAGCTGGATCTCGGGGCGATCGCCAAAGGCTATATCGCTGACCGCGTGCGC
GATTTTCTCCGTCAGCAGCAGGTGGAGAAGGCGCTGATCAACCTCGGCGGCAATGTGCATACCCTCGGCGAATGGGCCAT
TGGCCTGAAAAAACCATTTGCCGACGCGCAGGCGCTGATCGGTTCCCTGACGGTCAACGGGCAATCGGTAGTCACCTCCG
GGACCTACGAGCGCTATTTCGAACAGGATGGCAAACGCTGGCACCATATTCTCGACCCGCGCAGCGGTTATCCGCTGGAC
AACGAACTCGACAGCGTGACGGTCATTTCCGCGGATTCTTTAGACGGCGACATCTGGACCACGCTGCTGTTCGGCCTCGG
CGTGGAGAAGGGCTGCGCAGCGCTGCGTCAGCGGCAGGATATCGACGCGATTTTCGTTACCAAAAATCGCGACATTATTC
TCTCATCGCCGCAGCGGCTGCGTTTTGCGCCGCTGGACAGCGGCTATCGGGTTATTGACTGTACTGCTTAA

Upstream 100 bases:

>100_bases
GGCCATCAGGCGGCGAAACGTGCCAGAGGATAACCTGTAACCTTGCTCCTGCCGGGTTGGCGCTTTCACCGCCCGGCTTG
CCCCTTCTAAGGAGAGCCCG

Downstream 100 bases:

>100_bases
GCAAACTGTACTGTTCGGCGACCAGGCGGTAGCGATAGACCGGCCGCCCGGTAGCGCCGTAGTGGATAGTGGTATAGAGA
ATATTGATCTGCGCCAGCCA

Product: putative thiamine biosynthesis lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSDNRVYSYSAVLMGSPILLKLCSHDEAMASRVFQLIKRYEDLLTVNRAESQVMDINHAAGRHPVTVSRPVFQLIQCAKA
ASMVRDSAFNLAIGPLVKLWRIGFHGHSVPDAAEIRARLALTRPQEVILDEATCSVFLQQPGMELDLGAIAKGYIADRVR
DFLRQQQVEKALINLGGNVHTLGEWAIGLKKPFADAQALIGSLTVNGQSVVTSGTYERYFEQDGKRWHHILDPRSGYPLD
NELDSVTVISADSLDGDIWTTLLFGLGVEKGCAALRQRQDIDAIFVTKNRDIILSSPQRLRFAPLDSGYRVIDCTA

Sequences:

>Translated_316_residues
MSDNRVYSYSAVLMGSPILLKLCSHDEAMASRVFQLIKRYEDLLTVNRAESQVMDINHAAGRHPVTVSRPVFQLIQCAKA
ASMVRDSAFNLAIGPLVKLWRIGFHGHSVPDAAEIRARLALTRPQEVILDEATCSVFLQQPGMELDLGAIAKGYIADRVR
DFLRQQQVEKALINLGGNVHTLGEWAIGLKKPFADAQALIGSLTVNGQSVVTSGTYERYFEQDGKRWHHILDPRSGYPLD
NELDSVTVISADSLDGDIWTTLLFGLGVEKGCAALRQRQDIDAIFVTKNRDIILSSPQRLRFAPLDSGYRVIDCTA
>Mature_315_residues
SDNRVYSYSAVLMGSPILLKLCSHDEAMASRVFQLIKRYEDLLTVNRAESQVMDINHAAGRHPVTVSRPVFQLIQCAKAA
SMVRDSAFNLAIGPLVKLWRIGFHGHSVPDAAEIRARLALTRPQEVILDEATCSVFLQQPGMELDLGAIAKGYIADRVRD
FLRQQQVEKALINLGGNVHTLGEWAIGLKKPFADAQALIGSLTVNGQSVVTSGTYERYFEQDGKRWHHILDPRSGYPLDN
ELDSVTVISADSLDGDIWTTLLFGLGVEKGCAALRQRQDIDAIFVTKNRDIILSSPQRLRFAPLDSGYRVIDCTA

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=274, Percent_Identity=30.2919708029197, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 34992; Mature: 34860

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNRVYSYSAVLMGSPILLKLCSHDEAMASRVFQLIKRYEDLLTVNRAESQVMDINHAA
CCCCEEEEEEHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCC
GRHPVTVSRPVFQLIQCAKAASMVRDSAFNLAIGPLVKLWRIGFHGHSVPDAAEIRARLA
CCCCCEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LTRPQEVILDEATCSVFLQQPGMELDLGAIAKGYIADRVRDFLRQQQVEKALINLGGNVH
CCCCHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
TLGEWAIGLKKPFADAQALIGSLTVNGQSVVTSGTYERYFEQDGKRWHHILDPRSGYPLD
EHHHHHHHCCCCHHHHHHHHHHEEECCCEEEECCHHHHHHHHCCCHHHHHCCCCCCCCCC
NELDSVTVISADSLDGDIWTTLLFGLGVEKGCAALRQRQDIDAIFVTKNRDIILSSPQRL
CCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCEEEECCCCCE
RFAPLDSGYRVIDCTA
EEEECCCCCEEEEECC
>Mature Secondary Structure 
SDNRVYSYSAVLMGSPILLKLCSHDEAMASRVFQLIKRYEDLLTVNRAESQVMDINHAA
CCCEEEEEEHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCC
GRHPVTVSRPVFQLIQCAKAASMVRDSAFNLAIGPLVKLWRIGFHGHSVPDAAEIRARLA
CCCCCEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
LTRPQEVILDEATCSVFLQQPGMELDLGAIAKGYIADRVRDFLRQQQVEKALINLGGNVH
CCCCHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
TLGEWAIGLKKPFADAQALIGSLTVNGQSVVTSGTYERYFEQDGKRWHHILDPRSGYPLD
EHHHHHHHCCCCHHHHHHHHHHEEECCCEEEECCHHHHHHHHCCCHHHHHCCCCCCCCCC
NELDSVTVISADSLDGDIWTTLLFGLGVEKGCAALRQRQDIDAIFVTKNRDIILSSPQRL
CCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCEEEECCCCCE
RFAPLDSGYRVIDCTA
EEEECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]