| Definition | Klebsiella pneumoniae subsp. pneumoniae MGH 78578 chromosome, complete genome. |
|---|---|
| Accession | NC_009648 |
| Length | 5,315,120 |
Click here to switch to the map view.
The map label for this gene is topA [H]
Identifier: 152969824
GI number: 152969824
Start: 1424042
End: 1426639
Strand: Direct
Name: topA [H]
Synonym: KPN_01270
Alternate gene names: 152969824
Gene position: 1424042-1426639 (Clockwise)
Preceding gene: 152969822
Following gene: 152969825
Centisome position: 26.79
GC content: 57.7
Gene sequence:
>2598_bases ATGGGTAAAGCTCTCGTTATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAATAAGTATCTGGGTAATGACTACGTGGT GAAATCCAGCGTCGGTCATATCCGCGATTTGCCGACCAGTGGATCAGCTTCCAAAAAGAGCGCCGACTCTACCGCCACCA AAGGGGCTAAAAAGCCTAAAAAGGACGAACGTAGTGCTCTGGTTAACCGTATGGGCGTGGACCCATGGCATGACTGGAAT GCGCACTATGAAGTACTTCCGGGTAAAGAAAAGGTAGTTTCAGAACTCAAACAGTTGGCGGAAAAAGCTGACCACATCTA TCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCATTGCCTGGCACCTGCGGGAAGTCATCGGCGGCGATGAACAGCGTT ACAGCCGCGTGGTGTTCAATGAGATCACCAAAAACGCTATTCGTCAGGCCTTTGAAAAGCCGGGCGAACTGAATATCGAT CGGGTTAACGCCCAGCAGGCGCGTCGTTTTATGGACCGCGTGGTGGGCTATATGGTTTCGCCGCTGCTGTGGAAAAAAAT CGCCCGTGGTCTTTCTGCCGGGCGCGTGCAGTCCGTCGCGGTCCGCCTGGTGGTGGAGCGTGAACGTGAAATTAAAGCGT TCGTCCCGGAAGAGTACTGGGAAGTCGACGCCAGCACCACCACGCCGGGCGGTGACGCCCTGCCGCTGCAGGTGACCCAT AAGGACGATAAGCCGTTCCGTCCGGTCAGCCGCGATGAGACCATGGCGGCGGTATCGCTGCTGGAAAAAGCCAGCTACAG CGTACTGGAGCGTGAAGATAAGCCGACCAGCAGCAAGCCGGGCGCGCCGTTCATCACCTCCACCCTGCAGCAGGCGGCCA GCACCCGTCTGGGCTTCGGCGTGAAAAAGACCATGATGATGGCTCAGCGACTCTATGAGGCAGGGCACATCACCTATATG CGTACCGACTCCACCAACCTGAGTCAGGACGCGCTGAACATGGTTCGCGGCTACATCAGCGACAAGTTTGGCAAAAAATA TCTGCCGGATAGCGCGAACCAGTACGCCAGCAAAGAAAACTCGCAGGAAGCGCACGAAGCGATTCGTCCGTCTGATGTGA ACGTGCTGGCGGAAACGCTGAAAGATATGGAAGCGGATGCGCAGAAGCTTTATCAGCTGATCTGGCGCCAGTTTGTCGCC TGTCAGATGACGCCGGCGCAGTACGACTCCACCACGCTGACCGTGGCTGCGGGTGATTTCAAACTGAAAGCGCGCGGTCG CACTTTACGTTTCGACGGCTGGACAAAAGTCATGCCGGCGCTGCGCAAGGGCGATGAAGACCGGACGCTGCCGCTGGTCA AACAGGGCGACCGCCTGAGCCTGGTGGAGCTGACGCCTGCGCAGCACTTTACCAAGCCGCCGGCGCGTTTCAGCGAGGCC TCGCTGGTGAAAGAGCTGGAAAAACGCGGGATTGGCCGTCCGTCGACCTACGCGTCGATCATTTCGACCATTCAGGATCG CGGCTACGTGCGGGTGGAAAATCGTCGTTTTTACGCCGAAAAAATGGGCGAGATCGTTACCGACCGGCTGGAAGAAAACT TCCGCGATCTGATGAACTACGATTTTACCGCGCAGATGGAGGATCGCCTCGACCAGGTTGCGAACCACCAGGCCGAGTGG AAAGAGGTGCTGAACCACTTCTTCGGCGATTTTACCACCCAGCTGGCGACGGCAGAGAAAGATCCGGAAGAGGGCGGCAT GCAGCCGAACCCGATGGTGCTCACCAGCATCGACTGCCCGACCTGCGGCCGTAAAATGGGGATCCGTACCGCCAGCACCG GCGTTTTCCTCGGCTGTTCGGGCTATGCCTTGCCGCCGAAAGAGCGGTGCAAAACCACCATCAACCTGGTGCCGGAGAAC GAAGTCCTCAACGTGCTGGAAGGCGATGACGCGGAAACCAACGCGCTGCGCGCCAAACGTCGCTGCCAGAAGTGTGGGAC GGCGATGGACAGCTACCTTATCGATCCTAAGCGTAAGCTGCATGTCTGTGGTAATAACCCGACCTGCGACGGCTACGAGA TTGAAGAGGGCGAATTCCGCATCAAGGGCTATGATGGCCCGGTGGTGGAGTGCGAGAAGTGCGGGTCTGAAATGCACCTA AAAATGGGGCGTTTCGGTAAATACATGGCCTGTACCAACGACGAGTGTAAGAACACGCGTAAGATCCTGCGCAATGGCGA AGTGGCTCCGCCGAAGGAAGATCCGGTGCCGCTGCCGGAGCTGCCGTGTGAGAAGTCTGACGCCTACTTTGTGCTGCGCG ACGGGGCGGCGGGCGTCTTCCTGGCGGCCAACACCTTCCCGAAATCACGGGAAACGCGTGCGCCGCTGGTGGAGGAGCTG TACCGCTTCCGCGATCGTCTGCCGGAAAAACTGCGTTATCTCGCCGATGCGCCGCAGCAGGATCCGGAAGGCAATAAAAC GCTGGTACGCTTTAGCCGTAAAACCAAGCAGCAGTACGTGGCGTCTGAAAAAGAGGGCAAAGCGACCGGCTGGTCAGCTT TCTTTATCGACGGTAAGTGGACGGAAGCCAAAAAGTAA
Upstream 100 bases:
>100_bases ATATTCACCGACGGTGCCGCAACATATGAGGCAGTCGCCAGTGGAGGGTGTATGAATGTGCGATGTATTCCTGGATGAAT CAAATTAGGTAAAGGTGAAT
Downstream 100 bases:
>100_bases TGGCGTTCGTTCGGGCAGGAAAAGCGCGCTTTTCCTGCTCAGGCGACCGGCAGAGAGAGTGCATTTCTCTGCCGTGTCGT CTTCCCCTCTTTGCGTTAAT
Product: DNA topoisomerase I
Products: NA
Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]
Number of amino acids: Translated: 865; Mature: 864
Protein sequence:
>865_residues MGKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKSADSTATKGAKKPKKDERSALVNRMGVDPWHDWN AHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIAWHLREVIGGDEQRYSRVVFNEITKNAIRQAFEKPGELNID RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEYWEVDASTTTPGGDALPLQVTH KDDKPFRPVSRDETMAAVSLLEKASYSVLEREDKPTSSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGHITYM RTDSTNLSQDALNMVRGYISDKFGKKYLPDSANQYASKENSQEAHEAIRPSDVNVLAETLKDMEADAQKLYQLIWRQFVA CQMTPAQYDSTTLTVAAGDFKLKARGRTLRFDGWTKVMPALRKGDEDRTLPLVKQGDRLSLVELTPAQHFTKPPARFSEA SLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRDLMNYDFTAQMEDRLDQVANHQAEW KEVLNHFFGDFTTQLATAEKDPEEGGMQPNPMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPEN EVLNVLEGDDAETNALRAKRRCQKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPVVECEKCGSEMHL KMGRFGKYMACTNDECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVEEL YRFRDRLPEKLRYLADAPQQDPEGNKTLVRFSRKTKQQYVASEKEGKATGWSAFFIDGKWTEAKK
Sequences:
>Translated_865_residues MGKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKSADSTATKGAKKPKKDERSALVNRMGVDPWHDWN AHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIAWHLREVIGGDEQRYSRVVFNEITKNAIRQAFEKPGELNID RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEYWEVDASTTTPGGDALPLQVTH KDDKPFRPVSRDETMAAVSLLEKASYSVLEREDKPTSSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGHITYM RTDSTNLSQDALNMVRGYISDKFGKKYLPDSANQYASKENSQEAHEAIRPSDVNVLAETLKDMEADAQKLYQLIWRQFVA CQMTPAQYDSTTLTVAAGDFKLKARGRTLRFDGWTKVMPALRKGDEDRTLPLVKQGDRLSLVELTPAQHFTKPPARFSEA SLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRDLMNYDFTAQMEDRLDQVANHQAEW KEVLNHFFGDFTTQLATAEKDPEEGGMQPNPMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPEN EVLNVLEGDDAETNALRAKRRCQKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPVVECEKCGSEMHL KMGRFGKYMACTNDECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVEEL YRFRDRLPEKLRYLADAPQQDPEGNKTLVRFSRKTKQQYVASEKEGKATGWSAFFIDGKWTEAKK >Mature_864_residues GKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKSADSTATKGAKKPKKDERSALVNRMGVDPWHDWNA HYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIAWHLREVIGGDEQRYSRVVFNEITKNAIRQAFEKPGELNIDR VNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEYWEVDASTTTPGGDALPLQVTHK DDKPFRPVSRDETMAAVSLLEKASYSVLEREDKPTSSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGHITYMR TDSTNLSQDALNMVRGYISDKFGKKYLPDSANQYASKENSQEAHEAIRPSDVNVLAETLKDMEADAQKLYQLIWRQFVAC QMTPAQYDSTTLTVAAGDFKLKARGRTLRFDGWTKVMPALRKGDEDRTLPLVKQGDRLSLVELTPAQHFTKPPARFSEAS LVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRDLMNYDFTAQMEDRLDQVANHQAEWK EVLNHFFGDFTTQLATAEKDPEEGGMQPNPMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPENE VLNVLEGDDAETNALRAKRRCQKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPVVECEKCGSEMHLK MGRFGKYMACTNDECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVEELY RFRDRLPEKLRYLADAPQQDPEGNKTLVRFSRKTKQQYVASEKEGKATGWSAFFIDGKWTEAKK
Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]
COG id: COG0550
COG function: function code L; Topoisomerase IA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Homo sapiens, GI4507635, Length=608, Percent_Identity=24.8355263157895, Blast_Score=147, Evalue=3e-35, Organism=Homo sapiens, GI10835218, Length=575, Percent_Identity=25.7391304347826, Blast_Score=144, Evalue=3e-34, Organism=Escherichia coli, GI1787529, Length=865, Percent_Identity=92.7167630057804, Blast_Score=1690, Evalue=0.0, Organism=Escherichia coli, GI1788061, Length=647, Percent_Identity=23.9567233384853, Blast_Score=120, Evalue=4e-28, Organism=Caenorhabditis elegans, GI17555378, Length=527, Percent_Identity=25.8064516129032, Blast_Score=134, Evalue=2e-31, Organism=Caenorhabditis elegans, GI32563869, Length=515, Percent_Identity=25.4368932038835, Blast_Score=130, Evalue=2e-30, Organism=Saccharomyces cerevisiae, GI6323263, Length=487, Percent_Identity=24.435318275154, Blast_Score=106, Evalue=2e-23, Organism=Drosophila melanogaster, GI24585251, Length=613, Percent_Identity=23.8172920065253, Blast_Score=130, Evalue=3e-30, Organism=Drosophila melanogaster, GI24640096, Length=503, Percent_Identity=24.8508946322068, Blast_Score=122, Evalue=1e-27,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003601 - InterPro: IPR013497 - InterPro: IPR013824 - InterPro: IPR013825 - InterPro: IPR000380 - InterPro: IPR003602 - InterPro: IPR013498 - InterPro: IPR005733 - InterPro: IPR013263 - InterPro: IPR006171 [H]
Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]
EC number: =5.99.1.2 [H]
Molecular weight: Translated: 97298; Mature: 97166
Theoretical pI: Translated: 8.47; Mature: 8.47
Prosite motif: PS00396 TOPOISOMERASE_I_PROK
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.6 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 4.3 %Cys+Met (Translated Protein) 1.6 %Cys (Mature Protein) 2.5 %Met (Mature Protein) 4.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MGKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKSADSTATKGAKKPK CCCEEEEEECCCHHHHHHHHHCCCEEEECCCCHHHCCCCCCCCCCCCCCHHHHCCCCCCC KDERSALVNRMGVDPWHDWNAHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIA HHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHH WHLREVIGGDEQRYSRVVFNEITKNAIRQAFEKPGELNIDRVNAQQARRFMDRVVGYMVS HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHH PLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEYWEVDASTTTPGGDALPLQVTH HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEEEEEC KDDKPFRPVSRDETMAAVSLLEKASYSVLEREDKPTSSKPGAPFITSTLQQAASTRLGFG CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC VKKTMMMAQRLYEAGHITYMRTDSTNLSQDALNMVRGYISDKFGKKYLPDSANQYASKEN HHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCC SQEAHEAIRPSDVNVLAETLKDMEADAQKLYQLIWRQFVACQMTPAQYDSTTLTVAAGDF HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCE KLKARGRTLRFDGWTKVMPALRKGDEDRTLPLVKQGDRLSLVELTPAQHFTKPPARFSEA EEEECCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHHCCCCCCCHHHH SLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRDLMNY HHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC DFTAQMEDRLDQVANHQAEWKEVLNHFFGDFTTQLATAEKDPEEGGMQPNPMVLTSIDCP CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCC TCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPENEVLNVLEGDDAETNALRAKR CCCHHHCCEEECCCEEEECCCCCCCCHHHCCCEEEECCCHHHHHHHCCCCCHHHHHHHHH RCQKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPVVECEKCGSEMHL HHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCCEEEECCCCCCHHHHHCCCHHHH KMGRFGKYMACTNDECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVF HHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEE LAANTFPKSRETRAPLVEELYRFRDRLPEKLRYLADAPQQDPEGNKTLVRFSRKTKQQYV EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH ASEKEGKATGWSAFFIDGKWTEAKK HCCCCCCCCCEEEEEECCCCCCCCC >Mature Secondary Structure GKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKSADSTATKGAKKPK CCEEEEEECCCHHHHHHHHHCCCEEEECCCCHHHCCCCCCCCCCCCCCHHHHCCCCCCC KDERSALVNRMGVDPWHDWNAHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIA HHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHH WHLREVIGGDEQRYSRVVFNEITKNAIRQAFEKPGELNIDRVNAQQARRFMDRVVGYMVS HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHH PLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEYWEVDASTTTPGGDALPLQVTH HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEEEEEC KDDKPFRPVSRDETMAAVSLLEKASYSVLEREDKPTSSKPGAPFITSTLQQAASTRLGFG CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC VKKTMMMAQRLYEAGHITYMRTDSTNLSQDALNMVRGYISDKFGKKYLPDSANQYASKEN HHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCC SQEAHEAIRPSDVNVLAETLKDMEADAQKLYQLIWRQFVACQMTPAQYDSTTLTVAAGDF HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCE KLKARGRTLRFDGWTKVMPALRKGDEDRTLPLVKQGDRLSLVELTPAQHFTKPPARFSEA EEEECCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHHCCCCCCCHHHH SLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRDLMNY HHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC DFTAQMEDRLDQVANHQAEWKEVLNHFFGDFTTQLATAEKDPEEGGMQPNPMVLTSIDCP CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCC TCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPENEVLNVLEGDDAETNALRAKR CCCHHHCCEEECCCEEEECCCCCCCCHHHCCCEEEECCCHHHHHHHCCCCCHHHHHHHHH RCQKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPVVECEKCGSEMHL HHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEECCCCEEEECCCCCCHHHHHCCCHHHH KMGRFGKYMACTNDECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVF HHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEE LAANTFPKSRETRAPLVEELYRFRDRLPEKLRYLADAPQQDPEGNKTLVRFSRKTKQQYV EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH ASEKEGKATGWSAFFIDGKWTEAKK HCCCCCCCCCEEEEEECCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 3029379; 9097039; 9278503; 3032952; 8114910; 7779808 [H]