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The map label for this gene is celB [H]

Identifier: 152969785

GI number: 152969785

Start: 1382387

End: 1383640

Strand: Direct

Name: celB [H]

Synonym: KPN_01231

Alternate gene names: 152969785

Gene position: 1382387-1383640 (Clockwise)

Preceding gene: 152969784

Following gene: 152969786

Centisome position: 26.01

GC content: 57.5

Gene sequence:

>1254_bases
ATGCCGTTGACCCTCGCCGGGGCCATGTTTGTGTTAATTAACAACGTTTTTCTGAGCTTCGGCGAAGGCTCTTTCTTTTA
TTCCATGGGCATTCGCCTGGATGCCTCCACCATTGAAACCTTAAACGGCTTTAAGGCTATCGGCGGAAACGTCTATAACG
GCACCCTCGGGATTATGTCGCTAATGGCGCCGTTCTTTATCGGCTCCGCGCTGGCGGAAGAGCGCAAAGTTGACCCGATG
GCAGCTGGCCTGCTGGCGGTGGCGGCCTTCATGACCGTGACCCCGTATAGCGTGGGCGAAGCCTATGCGGTGGGCGCCAA
CTGGTTGGGCGGGCAGAACATCATCTCCGGGATGATTATCGGTCTGGTCGTTGCGGAACTGTTCACCTTCGTGATCCGTC
GCAACTGGGTGATTAGACTGCCGGACAGCGTGCCGGGGTCGGTATCACGCTCTTTCTCAGCGCTGATCCCGGGCTTCTTG
ATCCTCTCCATCTTCGGCATTATCTCGTGGGCCCTGGCGAGCTATGGCAGCAACTTCCACCAGATCATCATGGACTCTAT
CTCGACGCCGCTGGCGGCGATGGGCAGCGTGGTTGGCTGGGCATACGTTATCTTTAACTCTCTGCTGTGGTTCTTCGGTG
TGCACGGTTCTCTGGCGCTTACCGCCCTGGATAACGGCATCATGACGCCGTGGGCGCTGGAAAACATCGCGCTGTACAAC
CAGTACGGCTCTGTTGATGCGGCCATCGAAGCGGGCAAACAGTTCCACTTCTGGGCTAAGCCGATGCTCGACTCCTACAT
CCTGCTGGGTGGCTCCGGGGCGACGCTGGGTCTGATTATCGCCATCTTTATCGCTTCTCGCCGCGCTGACCATCGTCAGG
TTGCTAAGCTGGCGCTGCCGTCAGGCATCTTCCAGATTAACGAACCGATTCTGTTTGGTCTGCCGATCATCATGAACCCG
GTGATGTTCATCCCGTTCGTGCTGGTTCAGCCGATTCTGGCGGCCATTACGCTGGCGGCCTACAGCCTGGGGATTATCCC
ACCGGTGACCAACCTGGCGCCGTGGACTATGCCGACCGGTTTGGGAGCGTTCTTTAACAGTAACGGCAGCGTTGCCGCCC
TGCTGGTGGCCCTGTTCAACCTTGGCGTCGCTACCCTGGTCTACCTGCCGTTTGTGGTGCTGTCGAATAAAGCGCAGACC
GTTATCGAGCAGGAAGAGAGCGAAGAAGACATCGCTAACGCACTGAAATTCTGA

Upstream 100 bases:

>100_bases
TAAAGTGATCGATTCGCTTGAAAAGGTACTCCTTCCTTTTGCAGTGAAAATAGGAAAGCAGCCGCATATTAATGCAATAA
AAAATGGATTTATTAAATTA

Downstream 100 bases:

>100_bases
TACCGCGCGGTACGGGCAGCGTCCCGTACCGCCTGATAAATGAGGGAAATATTATGTTTGATCTGGACAACATCGACGCG
GTAGAGACCCCGGAAAATGA

Product: PTS system N,N'-diacetylchitobiose-specific transporter subunit IIC

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EIIC-Chb; PTS system N,N'-diacetylchitobiose-specific EIIC component [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MPLTLAGAMFVLINNVFLSFGEGSFFYSMGIRLDASTIETLNGFKAIGGNVYNGTLGIMSLMAPFFIGSALAEERKVDPM
AAGLLAVAAFMTVTPYSVGEAYAVGANWLGGQNIISGMIIGLVVAELFTFVIRRNWVIRLPDSVPGSVSRSFSALIPGFL
ILSIFGIISWALASYGSNFHQIIMDSISTPLAAMGSVVGWAYVIFNSLLWFFGVHGSLALTALDNGIMTPWALENIALYN
QYGSVDAAIEAGKQFHFWAKPMLDSYILLGGSGATLGLIIAIFIASRRADHRQVAKLALPSGIFQINEPILFGLPIIMNP
VMFIPFVLVQPILAAITLAAYSLGIIPPVTNLAPWTMPTGLGAFFNSNGSVAALLVALFNLGVATLVYLPFVVLSNKAQT
VIEQEESEEDIANALKF

Sequences:

>Translated_417_residues
MPLTLAGAMFVLINNVFLSFGEGSFFYSMGIRLDASTIETLNGFKAIGGNVYNGTLGIMSLMAPFFIGSALAEERKVDPM
AAGLLAVAAFMTVTPYSVGEAYAVGANWLGGQNIISGMIIGLVVAELFTFVIRRNWVIRLPDSVPGSVSRSFSALIPGFL
ILSIFGIISWALASYGSNFHQIIMDSISTPLAAMGSVVGWAYVIFNSLLWFFGVHGSLALTALDNGIMTPWALENIALYN
QYGSVDAAIEAGKQFHFWAKPMLDSYILLGGSGATLGLIIAIFIASRRADHRQVAKLALPSGIFQINEPILFGLPIIMNP
VMFIPFVLVQPILAAITLAAYSLGIIPPVTNLAPWTMPTGLGAFFNSNGSVAALLVALFNLGVATLVYLPFVVLSNKAQT
VIEQEESEEDIANALKF
>Mature_416_residues
PLTLAGAMFVLINNVFLSFGEGSFFYSMGIRLDASTIETLNGFKAIGGNVYNGTLGIMSLMAPFFIGSALAEERKVDPMA
AGLLAVAAFMTVTPYSVGEAYAVGANWLGGQNIISGMIIGLVVAELFTFVIRRNWVIRLPDSVPGSVSRSFSALIPGFLI
LSIFGIISWALASYGSNFHQIIMDSISTPLAAMGSVVGWAYVIFNSLLWFFGVHGSLALTALDNGIMTPWALENIALYNQ
YGSVDAAIEAGKQFHFWAKPMLDSYILLGGSGATLGLIIAIFIASRRADHRQVAKLALPSGIFQINEPILFGLPIIMNPV
MFIPFVLVQPILAAITLAAYSLGIIPPVTNLAPWTMPTGLGAFFNSNGSVAALLVALFNLGVATLVYLPFVVLSNKAQTV
IEQEESEEDIANALKF

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=417, Percent_Identity=87.5299760191847, Blast_Score=740, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: NA

Molecular weight: Translated: 44554; Mature: 44423

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTLAGAMFVLINNVFLSFGEGSFFYSMGIRLDASTIETLNGFKAIGGNVYNGTLGIMS
CCEEHHHHHHHHHHHHHHHCCCCCEEEEECCEECHHHHHHHHHHHHHCCCCCCHHHHHHH
LMAPFFIGSALAEERKVDPMAAGLLAVAAFMTVTPYSVGEAYAVGANWLGGQNIISGMII
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHH
GLVVAELFTFVIRRNWVIRLPDSVPGSVSRSFSALIPGFLILSIFGIISWALASYGSNFH
HHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
QIIMDSISTPLAAMGSVVGWAYVIFNSLLWFFGVHGSLALTALDNGIMTPWALENIALYN
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHH
QYGSVDAAIEAGKQFHFWAKPMLDSYILLGGSGATLGLIIAIFIASRRADHRQVAKLALP
CCCCHHHHHHCCCCEEEHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHCC
SGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYSLGIIPPVTNLAPWTMPTG
CCCCEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
LGAFFNSNGSVAALLVALFNLGVATLVYLPFVVLSNKAQTVIEQEESEEDIANALKF
CHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure 
PLTLAGAMFVLINNVFLSFGEGSFFYSMGIRLDASTIETLNGFKAIGGNVYNGTLGIMS
CEEHHHHHHHHHHHHHHHCCCCCEEEEECCEECHHHHHHHHHHHHHCCCCCCHHHHHHH
LMAPFFIGSALAEERKVDPMAAGLLAVAAFMTVTPYSVGEAYAVGANWLGGQNIISGMII
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHH
GLVVAELFTFVIRRNWVIRLPDSVPGSVSRSFSALIPGFLILSIFGIISWALASYGSNFH
HHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
QIIMDSISTPLAAMGSVVGWAYVIFNSLLWFFGVHGSLALTALDNGIMTPWALENIALYN
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHH
QYGSVDAAIEAGKQFHFWAKPMLDSYILLGGSGATLGLIIAIFIASRRADHRQVAKLALP
CCCCHHHHHHCCCCEEEHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHCC
SGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYSLGIIPPVTNLAPWTMPTG
CCCCEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
LGAFFNSNGSVAALLVALFNLGVATLVYLPFVVLSNKAQTVIEQEESEEDIANALKF
CHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2179047; 9097039; 9278503; 2092358; 9405618; 10913117 [H]