| Definition | Klebsiella pneumoniae subsp. pneumoniae MGH 78578 chromosome, complete genome. |
|---|---|
| Accession | NC_009648 |
| Length | 5,315,120 |
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The map label for this gene is topB [H]
Identifier: 152969762
GI number: 152969762
Start: 1358244
End: 1360190
Strand: Direct
Name: topB [H]
Synonym: KPN_01208
Alternate gene names: 152969762
Gene position: 1358244-1360190 (Clockwise)
Preceding gene: 152969761
Following gene: 152969763
Centisome position: 25.55
GC content: 62.04
Gene sequence:
>1947_bases ATGCGGTTGTTTATTGCAGAAAAACCGAGTCTCGGTCGGGCTATCGCCGATGTGTTGCCTAAACCGCACCGCAAAGGCGA TGGCTATATTGAGTGCGGCAACGGGCAGGTGGTCACCTGGTGTATCGGCCACCTGCTGGAGCAGGCGCAGCCGGACATCT ACGACAGCCGCTATGCCCGGTGGAATCTCAACGATCTGCCGATTGTGCCGGAGAAATGGCAGCTGCAGCCAAAGCCCTCG GTCACCAAACAGCTGAATGTCATCAAGCGCCTGCTCGGCGACGCCGAGGAGGTTATCCACGCCGGCGACCCGGACAGAGA AGGGCAGCTGCTGGTGGACGAAGTACTCGACTACCTGCAGCTGGCGCCGGAGAAGCGTCAGCAGGTGCAGCGCTGTCTGA TTAACGATCTCAACCCGCAGGCGGTGGAGCGGGCGATCAACCGTCTGCGCGCCAACAGCGAGTTCGTGCCGCTCTGCGTG TCGGCGCTGGCGCGGGCCCGCGCCGACTGGCTGTACGGCATCAATATGACCCGCGCCTACACCCTGCTGGGGCGCAACGC CGGCTATCAGGGGGTGCTCTCGGTGGGGCGGGTGCAGACCCCGGTACTTGGCCTGGTGGTCCGTCGCGATGAAGAGATTG AAAATTTCGTCGCCAAAGATTTCTTCGACGTGAAGGCGCATATCGTCACGCCGCAGGAGGAGCGTTTTGTCGCCACCTGG GTGCCGAGCGAAGCCTGCGAACCTTACCAGGATGAAGAAGGGCGCCTGCTGCATCGTCCGCTGGCCGAGCATGTGGTTAA GCGCATCGAAGGCCAGCCGGCAATCGTGACCGGCTATAACGATAAGCGCGATTCCGAACCCGCGCCGCTGCCGTTCTCGC TGTCGGCGCTGCAGATTGAGGCGGCCAAACGTTTCGGCTTCAGCGCGCAGAACGTGCTGGATATCTGCCAGAAGCTGTAC GAAACCCACAAACTCATCACCTATCCGCGTTCGGACAGCCGCTATCTGCCGGAAGAGCACTTCGCTGGCCGCCATGCGGT GTTGAATGCGATTGCCGTCCATGCGGCGGACCTGCTGCCGCAGCCGGTAGTGGATCCGGAGATCCGCAACCGCTGCTGGG ATGATAAAAAGGTCGACGCGCACCATGCGATTATCCCAACCGCCCGCAGCAGCCAGGTGAAGCTCACCGATAACGAAGCG AAGGTGTACACCCTGATCGCCCGCCAGTATCTGATGCAGTTCTGCCCTGACGCAGTGTTCCGCAAATGCCAGATTGATCT TGAGATCGCCAACGGCAAGTTTGTCGCCAAGGCGCGTTTTCTGGCCGAGGCGGGCTGGCGGACGCTGCTTGGCAGCAAAG AGCGCGATGAAGAGAACGACGGCACGCCGCTGCCGGTGGTGGCCAAAGGCGATGAACTGCTGTGCGAGAGGGGTGAGGTG GTGGAGCGTCAGACCCAGCCGCCGCGCCACTTTACCGACGCCACGCTGCTGTCGGCGATGACCGGGATTGCCCGCTTCGT GCAGGATAAAGATCTGAAGAAGATCCTCCGCGCCACCGACGGCCTCGGCACGGAAGCGACCCGCGCAGGGATTATCGAGC TGCTCTTCAAGCGTGGTTTCCTGAGCAAAAAGGGGCGCTATATCCACTCTTCGGAGGCCGGCAGGGCGCTCATTCATTCC CTGCCGGAGATGGCCGGGCGACCGGACATGACCGCCCACTGGGAGTCGGTGTTGACGCAGATCAGCGAGAAACAGTGCCG CTATCAGGACTTTATGCAGCCGCTGGTGGGGACGCTGTATCAACTGATCGATCAGGCGCGCAGCACGCCGGTGCGTCAGT TCCGCGGCCTGGTGGCGCCGGGCGGGGCGAAGAAGTCGTTCAGCAAAGGTAAAGGGAAGCCGAAAGGCAAAAAAGCGGCA GACGATACCGCGCCGCCGCCGCAGTAA
Upstream 100 bases:
>100_bases GTTCAGGCCGCCGCGGCGGAGTTCGGCATTAAGCTGGCCGCCATCGGCGAGCTGGTGACCGCCCGCGGCGGGCGACCCAT GATCGAGATCCGTTGATTCG
Downstream 100 bases:
>100_bases CCCGCCGGTGGTCGCGAGGATGCGTTATCCCGCGGCCACCGCTTCTCCGCCAGTCAGGCCAATGGTCTACACTAAACAGC CGGGAAGAGCCTGGACGCGG
Product: DNA topoisomerase III
Products: NA
Alternate protein names: DNA topoisomerase III [H]
Number of amino acids: Translated: 648; Mature: 648
Protein sequence:
>648_residues MRLFIAEKPSLGRAIADVLPKPHRKGDGYIECGNGQVVTWCIGHLLEQAQPDIYDSRYARWNLNDLPIVPEKWQLQPKPS VTKQLNVIKRLLGDAEEVIHAGDPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAINRLRANSEFVPLCV SALARARADWLYGINMTRAYTLLGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVAKDFFDVKAHIVTPQEERFVATW VPSEACEPYQDEEGRLLHRPLAEHVVKRIEGQPAIVTGYNDKRDSEPAPLPFSLSALQIEAAKRFGFSAQNVLDICQKLY ETHKLITYPRSDSRYLPEEHFAGRHAVLNAIAVHAADLLPQPVVDPEIRNRCWDDKKVDAHHAIIPTARSSQVKLTDNEA KVYTLIARQYLMQFCPDAVFRKCQIDLEIANGKFVAKARFLAEAGWRTLLGSKERDEENDGTPLPVVAKGDELLCERGEV VERQTQPPRHFTDATLLSAMTGIARFVQDKDLKKILRATDGLGTEATRAGIIELLFKRGFLSKKGRYIHSSEAGRALIHS LPEMAGRPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQARSTPVRQFRGLVAPGGAKKSFSKGKGKPKGKKAA DDTAPPPQ
Sequences:
>Translated_648_residues MRLFIAEKPSLGRAIADVLPKPHRKGDGYIECGNGQVVTWCIGHLLEQAQPDIYDSRYARWNLNDLPIVPEKWQLQPKPS VTKQLNVIKRLLGDAEEVIHAGDPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAINRLRANSEFVPLCV SALARARADWLYGINMTRAYTLLGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVAKDFFDVKAHIVTPQEERFVATW VPSEACEPYQDEEGRLLHRPLAEHVVKRIEGQPAIVTGYNDKRDSEPAPLPFSLSALQIEAAKRFGFSAQNVLDICQKLY ETHKLITYPRSDSRYLPEEHFAGRHAVLNAIAVHAADLLPQPVVDPEIRNRCWDDKKVDAHHAIIPTARSSQVKLTDNEA KVYTLIARQYLMQFCPDAVFRKCQIDLEIANGKFVAKARFLAEAGWRTLLGSKERDEENDGTPLPVVAKGDELLCERGEV VERQTQPPRHFTDATLLSAMTGIARFVQDKDLKKILRATDGLGTEATRAGIIELLFKRGFLSKKGRYIHSSEAGRALIHS LPEMAGRPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQARSTPVRQFRGLVAPGGAKKSFSKGKGKPKGKKAA DDTAPPPQ >Mature_648_residues MRLFIAEKPSLGRAIADVLPKPHRKGDGYIECGNGQVVTWCIGHLLEQAQPDIYDSRYARWNLNDLPIVPEKWQLQPKPS VTKQLNVIKRLLGDAEEVIHAGDPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAINRLRANSEFVPLCV SALARARADWLYGINMTRAYTLLGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVAKDFFDVKAHIVTPQEERFVATW VPSEACEPYQDEEGRLLHRPLAEHVVKRIEGQPAIVTGYNDKRDSEPAPLPFSLSALQIEAAKRFGFSAQNVLDICQKLY ETHKLITYPRSDSRYLPEEHFAGRHAVLNAIAVHAADLLPQPVVDPEIRNRCWDDKKVDAHHAIIPTARSSQVKLTDNEA KVYTLIARQYLMQFCPDAVFRKCQIDLEIANGKFVAKARFLAEAGWRTLLGSKERDEENDGTPLPVVAKGDELLCERGEV VERQTQPPRHFTDATLLSAMTGIARFVQDKDLKKILRATDGLGTEATRAGIIELLFKRGFLSKKGRYIHSSEAGRALIHS LPEMAGRPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQARSTPVRQFRGLVAPGGAKKSFSKGKGKPKGKKAA DDTAPPPQ
Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]
COG id: COG0550
COG function: function code L; Topoisomerase IA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Homo sapiens, GI4507635, Length=619, Percent_Identity=21.8093699515347, Blast_Score=107, Evalue=3e-23, Organism=Homo sapiens, GI10835218, Length=617, Percent_Identity=23.8249594813614, Blast_Score=93, Evalue=6e-19, Organism=Escherichia coli, GI1788061, Length=645, Percent_Identity=89.6124031007752, Blast_Score=1203, Evalue=0.0, Organism=Escherichia coli, GI1787529, Length=549, Percent_Identity=24.408014571949, Blast_Score=128, Evalue=1e-30, Organism=Caenorhabditis elegans, GI32563869, Length=628, Percent_Identity=21.8152866242038, Blast_Score=99, Evalue=7e-21, Organism=Caenorhabditis elegans, GI17555378, Length=646, Percent_Identity=22.2910216718266, Blast_Score=96, Evalue=4e-20, Organism=Drosophila melanogaster, GI24640096, Length=631, Percent_Identity=22.3454833597464, Blast_Score=96, Evalue=5e-20, Organism=Drosophila melanogaster, GI24585251, Length=529, Percent_Identity=24.952741020794, Blast_Score=92, Evalue=8e-19,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003601 - InterPro: IPR013497 - InterPro: IPR013824 - InterPro: IPR013825 - InterPro: IPR000380 - InterPro: IPR003602 - InterPro: IPR005738 - InterPro: IPR006171 [H]
Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]
EC number: =5.99.1.2 [H]
Molecular weight: Translated: 72751; Mature: 72751
Theoretical pI: Translated: 8.33; Mature: 8.33
Prosite motif: PS00396 TOPOISOMERASE_I_PROK
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.7 %Cys (Translated Protein) 1.1 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 1.7 %Cys (Mature Protein) 1.1 %Met (Mature Protein) 2.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRLFIAEKPSLGRAIADVLPKPHRKGDGYIECGNGQVVTWCIGHLLEQAQPDIYDSRYAR CEEEEECCCCHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCHHCCCCCC WNLNDLPIVPEKWQLQPKPSVTKQLNVIKRLLGDAEEVIHAGDPDREGQLLVDEVLDYLQ CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHH LAPEKRQQVQRCLINDLNPQAVERAINRLRANSEFVPLCVSALARARADWLYGINMTRAY CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHH TLLGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVAKDFFDVKAHIVTPQEERFVATW HHHCCCCCCHHHHHHCCHHCCHHHHHHHCHHHHHHHHHHHHHHCCEEEECCCCCCEEEEE VPSEACEPYQDEEGRLLHRPLAEHVVKRIEGQPAIVTGYNDKRDSEPAPLPFSLSALQIE CCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHHH AAKRFGFSAQNVLDICQKLYETHKLITYPRSDSRYLPEEHFAGRHAVLNAIAVHAADLLP HHHHCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCC QPVVDPEIRNRCWDDKKVDAHHAIIPTARSSQVKLTDNEAKVYTLIARQYLMQFCPDAVF CCCCCHHHHHCCCCCCCCCCCCEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHH RKCQIDLEIANGKFVAKARFLAEAGWRTLLGSKERDEENDGTPLPVVAKGDELLCERGEV HHHEEEEEECCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHCCHH VERQTQPPRHFTDATLLSAMTGIARFVQDKDLKKILRATDGLGTEATRAGIIELLFKRGF HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC LSKKGRYIHSSEAGRALIHSLPEMAGRPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLY CCCCCCEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH QLIDQARSTPVRQFRGLVAPGGAKKSFSKGKGKPKGKKAADDTAPPPQ HHHHHHHCCHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCC >Mature Secondary Structure MRLFIAEKPSLGRAIADVLPKPHRKGDGYIECGNGQVVTWCIGHLLEQAQPDIYDSRYAR CEEEEECCCCHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCHHCCCCCC WNLNDLPIVPEKWQLQPKPSVTKQLNVIKRLLGDAEEVIHAGDPDREGQLLVDEVLDYLQ CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHH LAPEKRQQVQRCLINDLNPQAVERAINRLRANSEFVPLCVSALARARADWLYGINMTRAY CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHH TLLGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVAKDFFDVKAHIVTPQEERFVATW HHHCCCCCCHHHHHHCCHHCCHHHHHHHCHHHHHHHHHHHHHHCCEEEECCCCCCEEEEE VPSEACEPYQDEEGRLLHRPLAEHVVKRIEGQPAIVTGYNDKRDSEPAPLPFSLSALQIE CCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHHH AAKRFGFSAQNVLDICQKLYETHKLITYPRSDSRYLPEEHFAGRHAVLNAIAVHAADLLP HHHHCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCC QPVVDPEIRNRCWDDKKVDAHHAIIPTARSSQVKLTDNEAKVYTLIARQYLMQFCPDAVF CCCCCHHHHHCCCCCCCCCCCCEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCHHHH RKCQIDLEIANGKFVAKARFLAEAGWRTLLGSKERDEENDGTPLPVVAKGDELLCERGEV HHHEEEEEECCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHCCHH VERQTQPPRHFTDATLLSAMTGIARFVQDKDLKKILRATDGLGTEATRAGIIELLFKRGF HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC LSKKGRYIHSSEAGRALIHSLPEMAGRPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLY CCCCCCEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH QLIDQARSTPVRQFRGLVAPGGAKKSFSKGKGKPKGKKAADDTAPPPQ HHHHHHHCCHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 2553698; 9097039; 9278503; 10574789 [H]