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The map label for this gene is sucB [H]

Identifier: 152969297

GI number: 152969297

Start: 824637

End: 825863

Strand: Direct

Name: sucB [H]

Synonym: KPN_00733

Alternate gene names: 152969297

Gene position: 824637-825863 (Clockwise)

Preceding gene: 152969296

Following gene: 152969298

Centisome position: 15.51

GC content: 59.49

Gene sequence:

>1227_bases
ATGAGTAGCGTAGATATTCTGGTTCCCGACCTGCCTGAGTCCGTAGCTGACGCAACCGTCGCCACCTGGCACAAAAAACC
GGGCGACGCGGTCGTCCGTGACGAAGTTCTGGTCGAAATCGAAACTGACAAAGTGGTACTGGAAGTACCGGCATCAGCGG
ACGGCATTCTGGATGCAGTGCTGGAAGACGAGGGCGCCACCGTCCTGTCTCGCCAGATCCTTGGCCGCCTGCGCGAAGGC
AACAGCGCAGGTAAAGAAAGCAGCGAGAAAGCCGATGCGAAAGCGTCCACTCCGGCGCAGCGTCAGCAGGCCTCTCTGGA
AGAGCAGAACAACGACGCGCTGAGCCCGGCGATCCGTCGTCTGCTGGCTGAACACAACCTTGACCCAGCGGCCATCAAAG
GCACTGGCGTCGGCGGCCGTCTGACCCGCGAAGACGTTGAGAAACATCTGGCGAAAGCCCCGGCGCCGGCAGAAGCCAAA
GCGCCAGCCGCCGCCCCGGCAGCCGCGCCGGCGCCGCAGCTGGGCCACCGTTCGGAAAAACGCGTTCCGATGACCCGTCT
GCGTAAACGCGTAGCCGAACGTCTGCTGGAAGCGAAAAACTCTACCGCCATGCTGACGACCTTTAACGAAGTCAACATGA
AGCCCATCATGGATCTGCGTAAGCAGTACGGTGAGGCGTTCGAAAAACGTCATGGCATCCGCCTGGGCTTTATGTCCTTC
TACGTCAAAGCGGTAGTGGAAGCGCTGAAACGCTATCCGGAAGTGAACGCTTCCATCGATGGCGACGACGTGGTGTACCA
CAACTACTTCGACGTCAGCATGGCCGTATCCACTCCGCGCGGTCTGGTCACCCCGGTGCTGCGTGACGTCGACCTGCTGG
GCATGGCGGATATCGAAAAGAATATCAAAGAGCTGGCAGTGAAAGGCCGCGACGGCAAGCTGACCGTAGACGACCTGACC
GGCGGTAACTTCACTATTACCAACGGCGGCGTCTTCGGCTCCCTGATGTCCACGCCGATCATCAACCCACCGCAGAGCGC
TATTCTGGGTATGCATGCCATTAAAGACCGTCCGATGGCGGTCAATGGCAAGGTAGAGATCCTGCCGATGATGTACCTGG
CGCTCTCCTATGACCACCGTCTGATCGACGGACGTGAATCGGTAGGCTTCCTGGTGGCGATTAAAGAGCTGCTGGAAGAT
CCGACCCGTCTGCTGCTGGACGTGTAG

Upstream 100 bases:

>100_bases
CCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTCAATGACGCGCTGAACGTC
GATTAATTAAAGGATACACA

Downstream 100 bases:

>100_bases
TCAGCAAGCGTATTACCTACCGTAGGCCGGATAACACGCGTTAGCGCCGTTATCAGGCCTACAGGTTTAAAGATAATTAC
ACCCTGAAGGATGGACTGAA

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAVLEDEGATVLSRQILGRLREG
NSAGKESSEKADAKASTPAQRQQASLEEQNNDALSPAIRRLLAEHNLDPAAIKGTGVGGRLTREDVEKHLAKAPAPAEAK
APAAAPAAAPAPQLGHRSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEAFEKRHGIRLGFMSF
YVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDLLGMADIEKNIKELAVKGRDGKLTVDDLT
GGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVNGKVEILPMMYLALSYDHRLIDGRESVGFLVAIKELLED
PTRLLLDV

Sequences:

>Translated_408_residues
MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAVLEDEGATVLSRQILGRLREG
NSAGKESSEKADAKASTPAQRQQASLEEQNNDALSPAIRRLLAEHNLDPAAIKGTGVGGRLTREDVEKHLAKAPAPAEAK
APAAAPAAAPAPQLGHRSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEAFEKRHGIRLGFMSF
YVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDLLGMADIEKNIKELAVKGRDGKLTVDDLT
GGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVNGKVEILPMMYLALSYDHRLIDGRESVGFLVAIKELLED
PTRLLLDV
>Mature_407_residues
SSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAVLEDEGATVLSRQILGRLREGN
SAGKESSEKADAKASTPAQRQQASLEEQNNDALSPAIRRLLAEHNLDPAAIKGTGVGGRLTREDVEKHLAKAPAPAEAKA
PAAAPAAAPAPQLGHRSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEAFEKRHGIRLGFMSFY
VKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDLLGMADIEKNIKELAVKGRDGKLTVDDLTG
GNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVNGKVEILPMMYLALSYDHRLIDGRESVGFLVAIKELLEDP
TRLLLDV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=251, Percent_Identity=58.1673306772908, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI31711992, Length=435, Percent_Identity=30.5747126436782, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=28.6031042128603, Blast_Score=172, Evalue=7e-43,
Organism=Homo sapiens, GI203098816, Length=451, Percent_Identity=28.6031042128603, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI110671329, Length=425, Percent_Identity=27.2941176470588, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI260898739, Length=168, Percent_Identity=34.5238095238095, Blast_Score=105, Evalue=9e-23,
Organism=Escherichia coli, GI1786946, Length=408, Percent_Identity=92.6470588235294, Blast_Score=739, Evalue=0.0,
Organism=Escherichia coli, GI1786305, Length=407, Percent_Identity=31.9410319410319, Blast_Score=171, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI25146366, Length=415, Percent_Identity=41.4457831325301, Blast_Score=310, Evalue=9e-85,
Organism=Caenorhabditis elegans, GI17560088, Length=445, Percent_Identity=29.8876404494382, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=420, Percent_Identity=25, Blast_Score=158, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=312, Percent_Identity=31.4102564102564, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320352, Length=409, Percent_Identity=41.8092909535452, Blast_Score=315, Evalue=9e-87,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=27.6169265033408, Blast_Score=146, Evalue=6e-36,
Organism=Drosophila melanogaster, GI18859875, Length=434, Percent_Identity=28.8018433179723, Blast_Score=161, Evalue=6e-40,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44215; Mature: 44084

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAV
CCCCCEECCCCCHHHHHHHHHHHCCCCCCCEEECEEEEEEECCEEEEECCCCCCHHHHHH
LEDEGATVLSRQILGRLREGNSAGKESSEKADAKASTPAQRQQASLEEQNNDALSPAIRR
HCCCCHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHH
LLAEHNLDPAAIKGTGVGGRLTREDVEKHLAKAPAPAEAKAPAAAPAAAPAPQLGHRSEK
HHHHCCCCCCEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEAFEKRHGIRLGFMSF
CCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHH
YVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDLLGMADIEK
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
NIKELAVKGRDGKLTVDDLTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMA
HHHHHHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEE
VNGKVEILPMMYLALSYDHRLIDGRESVGFLVAIKELLEDPTRLLLDV
ECCCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCC
>Mature Secondary Structure 
SSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAV
CCCCEECCCCCHHHHHHHHHHHCCCCCCCEEECEEEEEEECCEEEEECCCCCCHHHHHH
LEDEGATVLSRQILGRLREGNSAGKESSEKADAKASTPAQRQQASLEEQNNDALSPAIRR
HCCCCHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHH
LLAEHNLDPAAIKGTGVGGRLTREDVEKHLAKAPAPAEAKAPAAAPAAAPAPQLGHRSEK
HHHHCCCCCCEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEAFEKRHGIRLGFMSF
CCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHH
YVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDLLGMADIEK
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
NIKELAVKGRDGKLTVDDLTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMA
HHHHHHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEE
VNGKVEILPMMYLALSYDHRLIDGRESVGFLVAIKELLEDPTRLLLDV
ECCCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]