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The map label for this gene is phrB [H]

Identifier: 152969283

GI number: 152969283

Start: 806682

End: 808124

Strand: Direct

Name: phrB [H]

Synonym: KPN_00719

Alternate gene names: 152969283

Gene position: 806682-808124 (Clockwise)

Preceding gene: 152969282

Following gene: 152969285

Centisome position: 15.18

GC content: 62.72

Gene sequence:

>1443_bases
ATGGCCACCCATCTGGTCTGGTTACGCACGGATTTACGCATTCACGATAACCTGGCGCTGGCTGCCGCCTGCCGCGATCC
GCAGGCGCAGGTGCTGGCGCTGTATATCGCCACCCCGGGGCAGTGGCGTGAACATCACCTGGCGCCACGGCAGGCCGCTT
TTATCGCCAGCCATTTACAGAGTCTGCATGCCGCGCTGGCGGAACGGGGCATACCGCTGTGGGTAGAAGAGGCGGACGAT
TTTACCGCCAGCGTCGAACGGCTGGCCGATTTTTGCCAGCAGCATCAGGTCAGTCACCTGTTCTACAACTATCAGTACGA
ATTCAATGAACGCCAGCGCGATGCCGCCGTTGAGAACACCTTGTGCGACGTGATTTGCCAGGGCTTTGACGACAGCGTCC
TGTTGCCCCCCGGCAGCGTACTGACCGGTGGCGGCGAAATGTATAAAGTCTTCACCCCGTTTAAAAATGCGTTTATTCGC
CGTCTGCGGGACGGGCTGCCGGCGTGCGTGGCGGCCCCGAAGCCGCGCCAGGCGCCTGCCCGCCAGGCGCCGCCGCTGCC
TGAGCTTAACTACCCGCAAACGCCGTTTGACGGGCTGCTGTTTGCCGCGGATGAAAAAACGGCGCTGGCGCGTCTGCGCG
CGTTTTGTCAGCAAGCGGCGGCGGATTACGAGGGGCAGCGCGACTTCCCGGCCGTGGAAGGGACCAGTCGCCTGTCGCCC
TGCCTGGCCATCGGCGTGCTGTCCCCCCGCCAGTGCCTGCATCGTCTGCTGACGGAACATCCCGCCGCGCTGGACGGCGG
AGCCGGCGCCACCTGGCTTAACGAGCTGATCTGGCGCGAGTTTTACCGCCATCTGATGGTCTACTATCCGAAGCTCTGTA
AAGGGCGGCCATTTACTGCCTGGACAGATAAGGTCGCCTGGCGCGCAGAGGAGGCCTCCCTACAGGCCTGGCAGCGCGGG
GAGACCGGTTTTCCGATTGTTGATGCCGCCATGCGCCAGCTTAACGCCACCGGCTGGATGCATAACCGGTTGCGCATGAT
TGTAGCCAGCTTCCTGACCAAAGATTTGCGCCTCGACTGGCGGGCGGGCGAGCGCTATTTCATGAGCCAGCTGATTGATG
GCGACCTGGCGGCGAATAACGGCGGCTGGCAGTGGGCGGCTTCAACGGGCACCGACGCCGCGCCCTATTTCCGCATATTT
AATCCCACCACCCAGGGCGAAAAGTTTGATAAACAGGGGGTGTTTATACGTCGCTGGCTGCCGGAGCTGGCAAAGGTGCC
GGAGAAGGCCCTGCATCAGCCGTGGGCGTGGGCGGATAAACAAGGGATCACGCTGGATTATTCGCGCCCGATAGTTGATC
ACAAGCAGGCGCGGCAGGAGACGCTGGCGGCCTGGGAGGCCGCCAGAAAAGGAATTACTCCACCGCCAGACGGCGGGTCC
TGA

Upstream 100 bases:

>100_bases
CGCCGCCTTGGAATTGGCAGGTGCGTATTTTGCCGGAGCGCGCTATTTTTGCCGGCTTAGATCATAAGATGAGTATTGCT
GACGGATAATAAGGAGTGAT

Downstream 100 bases:

>100_bases
CTTTCAGCGAATGATACAGCCAGATGACCAGCACCACGCCGACGCAGGCCAGCGCGCCCCAGGTGATCTGGCTGAAGACG
GTAATGTAGGCGTCAATGGA

Product: deoxyribodipyrimidine photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MATHLVWLRTDLRIHDNLALAAACRDPQAQVLALYIATPGQWREHHLAPRQAAFIASHLQSLHAALAERGIPLWVEEADD
FTASVERLADFCQQHQVSHLFYNYQYEFNERQRDAAVENTLCDVICQGFDDSVLLPPGSVLTGGGEMYKVFTPFKNAFIR
RLRDGLPACVAAPKPRQAPARQAPPLPELNYPQTPFDGLLFAADEKTALARLRAFCQQAAADYEGQRDFPAVEGTSRLSP
CLAIGVLSPRQCLHRLLTEHPAALDGGAGATWLNELIWREFYRHLMVYYPKLCKGRPFTAWTDKVAWRAEEASLQAWQRG
ETGFPIVDAAMRQLNATGWMHNRLRMIVASFLTKDLRLDWRAGERYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRIF
NPTTQGEKFDKQGVFIRRWLPELAKVPEKALHQPWAWADKQGITLDYSRPIVDHKQARQETLAAWEAARKGITPPPDGGS

Sequences:

>Translated_480_residues
MATHLVWLRTDLRIHDNLALAAACRDPQAQVLALYIATPGQWREHHLAPRQAAFIASHLQSLHAALAERGIPLWVEEADD
FTASVERLADFCQQHQVSHLFYNYQYEFNERQRDAAVENTLCDVICQGFDDSVLLPPGSVLTGGGEMYKVFTPFKNAFIR
RLRDGLPACVAAPKPRQAPARQAPPLPELNYPQTPFDGLLFAADEKTALARLRAFCQQAAADYEGQRDFPAVEGTSRLSP
CLAIGVLSPRQCLHRLLTEHPAALDGGAGATWLNELIWREFYRHLMVYYPKLCKGRPFTAWTDKVAWRAEEASLQAWQRG
ETGFPIVDAAMRQLNATGWMHNRLRMIVASFLTKDLRLDWRAGERYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRIF
NPTTQGEKFDKQGVFIRRWLPELAKVPEKALHQPWAWADKQGITLDYSRPIVDHKQARQETLAAWEAARKGITPPPDGGS
>Mature_479_residues
ATHLVWLRTDLRIHDNLALAAACRDPQAQVLALYIATPGQWREHHLAPRQAAFIASHLQSLHAALAERGIPLWVEEADDF
TASVERLADFCQQHQVSHLFYNYQYEFNERQRDAAVENTLCDVICQGFDDSVLLPPGSVLTGGGEMYKVFTPFKNAFIRR
LRDGLPACVAAPKPRQAPARQAPPLPELNYPQTPFDGLLFAADEKTALARLRAFCQQAAADYEGQRDFPAVEGTSRLSPC
LAIGVLSPRQCLHRLLTEHPAALDGGAGATWLNELIWREFYRHLMVYYPKLCKGRPFTAWTDKVAWRAEEASLQAWQRGE
TGFPIVDAAMRQLNATGWMHNRLRMIVASFLTKDLRLDWRAGERYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRIFN
PTTQGEKFDKQGVFIRRWLPELAKVPEKALHQPWAWADKQGITLDYSRPIVDHKQARQETLAAWEAARKGITPPPDGGS

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=488, Percent_Identity=27.6639344262295, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI188536103, Length=394, Percent_Identity=28.4263959390863, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI4758072, Length=481, Percent_Identity=27.027027027027, Blast_Score=136, Evalue=4e-32,
Organism=Escherichia coli, GI1786926, Length=471, Percent_Identity=69.8513800424628, Blast_Score=703, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324962, Length=508, Percent_Identity=31.2992125984252, Blast_Score=253, Evalue=6e-68,
Organism=Drosophila melanogaster, GI17137248, Length=508, Percent_Identity=26.5748031496063, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24585455, Length=508, Percent_Identity=26.5748031496063, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24648152, Length=539, Percent_Identity=27.0871985157699, Blast_Score=132, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 54055; Mature: 53924

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATHLVWLRTDLRIHDNLALAAACRDPQAQVLALYIATPGQWREHHLAPRQAAFIASHLQ
CCCEEEEEEECCEEECCEEEEECCCCCCCEEEEEEEECCCCHHHHCCCHHHHHHHHHHHH
SLHAALAERGIPLWVEEADDFTASVERLADFCQQHQVSHLFYNYQYEFNERQRDAAVENT
HHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHH
LCDVICQGFDDSVLLPPGSVLTGGGEMYKVFTPFKNAFIRRLRDGLPACVAAPKPRQAPA
HHHHHHCCCCCCEECCCCCEEECCCCEEEEEHHHHHHHHHHHHCCCCEECCCCCCCCCCC
RQAPPLPELNYPQTPFDGLLFAADEKTALARLRAFCQQAAADYEGQRDFPAVEGTSRLSP
CCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCH
CLAIGVLSPRQCLHRLLTEHPAALDGGAGATWLNELIWREFYRHLMVYYPKLCKGRPFTA
HHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
WTDKVAWRAEEASLQAWQRGETGFPIVDAAMRQLNATGWMHNRLRMIVASFLTKDLRLDW
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RAGERYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRIFNPTTQGEKFDKQGVFIRRWL
CCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHH
PELAKVPEKALHQPWAWADKQGITLDYSRPIVDHKQARQETLAAWEAARKGITPPPDGGS
HHHHHCCHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ATHLVWLRTDLRIHDNLALAAACRDPQAQVLALYIATPGQWREHHLAPRQAAFIASHLQ
CCEEEEEEECCEEECCEEEEECCCCCCCEEEEEEEECCCCHHHHCCCHHHHHHHHHHHH
SLHAALAERGIPLWVEEADDFTASVERLADFCQQHQVSHLFYNYQYEFNERQRDAAVENT
HHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHH
LCDVICQGFDDSVLLPPGSVLTGGGEMYKVFTPFKNAFIRRLRDGLPACVAAPKPRQAPA
HHHHHHCCCCCCEECCCCCEEECCCCEEEEEHHHHHHHHHHHHCCCCEECCCCCCCCCCC
RQAPPLPELNYPQTPFDGLLFAADEKTALARLRAFCQQAAADYEGQRDFPAVEGTSRLSP
CCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCH
CLAIGVLSPRQCLHRLLTEHPAALDGGAGATWLNELIWREFYRHLMVYYPKLCKGRPFTA
HHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
WTDKVAWRAEEASLQAWQRGETGFPIVDAAMRQLNATGWMHNRLRMIVASFLTKDLRLDW
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RAGERYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRIFNPTTQGEKFDKQGVFIRRWL
CCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHH
PELAKVPEKALHQPWAWADKQGITLDYSRPIVDHKQARQETLAAWEAARKGITPPPDGGS
HHHHHCCHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6325460; 8905232; 9278503; 2200511; 7604260 [H]