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The map label for this gene is seqA [H]

Identifier: 152969274

GI number: 152969274

Start: 795502

End: 796050

Strand: Direct

Name: seqA [H]

Synonym: KPN_00710

Alternate gene names: 152969274

Gene position: 795502-796050 (Clockwise)

Preceding gene: 152969268

Following gene: 152969275

Centisome position: 14.97

GC content: 54.46

Gene sequence:

>549_bases
ATGAAAACAATTGAAGTTGATGATGAACTCTATCGCTATATTGCCAGCCACACTCTGCATATCGGCGAGAGCGCATCCGA
CATTTTACGGCGTATGCTGAAATTTTCCGCCGTTTCCCAACCGGCTACACCGGTCAGCAAAGCCGCGCCGACGCTGGCGC
CAGCGCCTGTCGCTGAGGTGAAACCCGCTAACCCGGTGAAAGATAAAGTCCGCGCCATGCGCGAGATGCTGTTATCTGAT
GAATACGCCGAGCAGAAGCGCGCGGTGAACCGTTTTATGCTGGTGTTGACGACCCTCTACTCCCTGGACAGTAAAGCATT
TGCCGAGGCCACCGAGTCTCTGCACGGCCGCACGCGCGTTTACTTCGCGGAAGATGCGCGTACGCTGCTGAAAAGCGGTA
ATCAGACCAAACCGAAACAGGTTCCTGGTACACCGTGGTGGGTGATCACCAATACCAATACCGGCCGTAAATGCAGCATG
ATTGAGCACATCATGCAGTCGATGCAGTTCCCTGCGGAGCTGATTGAAAAGGTTTGCGGCACGATCTAG

Upstream 100 bases:

>100_bases
TGAGTTTTACCGCCATCCTGGTTTGACGCTATCCGCGGTTGGGATTTGTACTTATAATCCCAACGATTTGTATTCAGAAA
AGATATCGCACTGGATTAAG

Downstream 100 bases:

>100_bases
CTTTTTAACATTTGCACCAGAAGGATCAGGCAATGGCAATCGATAAACGTGCGGGGCAGCCTGCGCAACAGAGTGATTTG
ATTAACGTCGCCCAACTGAC

Product: replication initiation regulator SeqA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MKTIEVDDELYRYIASHTLHIGESASDILRRMLKFSAVSQPATPVSKAAPTLAPAPVAEVKPANPVKDKVRAMREMLLSD
EYAEQKRAVNRFMLVLTTLYSLDSKAFAEATESLHGRTRVYFAEDARTLLKSGNQTKPKQVPGTPWWVITNTNTGRKCSM
IEHIMQSMQFPAELIEKVCGTI

Sequences:

>Translated_182_residues
MKTIEVDDELYRYIASHTLHIGESASDILRRMLKFSAVSQPATPVSKAAPTLAPAPVAEVKPANPVKDKVRAMREMLLSD
EYAEQKRAVNRFMLVLTTLYSLDSKAFAEATESLHGRTRVYFAEDARTLLKSGNQTKPKQVPGTPWWVITNTNTGRKCSM
IEHIMQSMQFPAELIEKVCGTI
>Mature_182_residues
MKTIEVDDELYRYIASHTLHIGESASDILRRMLKFSAVSQPATPVSKAAPTLAPAPVAEVKPANPVKDKVRAMREMLLSD
EYAEQKRAVNRFMLVLTTLYSLDSKAFAEATESLHGRTRVYFAEDARTLLKSGNQTKPKQVPGTPWWVITNTNTGRKCSM
IEHIMQSMQFPAELIEKVCGTI

Specific function: Negative regulator of replication initiation. Required for sequestration, a process that blocks secondary initiation events. Could block the allosteric transition of dnaA from an inactive form to an active form. Binds hemimethylated oriC. This process has

COG id: COG3057

COG function: function code L; Negative regulator of replication initiationR

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786903, Length=182, Percent_Identity=83.5164835164835, Blast_Score=321, Evalue=2e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005621
- InterPro:   IPR010985 [H]

Pfam domain/function: PF03925 SeqA [H]

EC number: NA

Molecular weight: Translated: 20369; Mature: 20369

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIEVDDELYRYIASHTLHIGESASDILRRMLKFSAVSQPATPVSKAAPTLAPAPVAEV
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCC
KPANPVKDKVRAMREMLLSDEYAEQKRAVNRFMLVLTTLYSLDSKAFAEATESLHGRTRV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEEE
YFAEDARTLLKSGNQTKPKQVPGTPWWVITNTNTGRKCSMIEHIMQSMQFPAELIEKVCG
EEHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHC
TI
CC
>Mature Secondary Structure
MKTIEVDDELYRYIASHTLHIGESASDILRRMLKFSAVSQPATPVSKAAPTLAPAPVAEV
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCC
KPANPVKDKVRAMREMLLSDEYAEQKRAVNRFMLVLTTLYSLDSKAFAEATESLHGRTRV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEEE
YFAEDARTLLKSGNQTKPKQVPGTPWWVITNTNTGRKCSMIEHIMQSMQFPAELIEKVCG
EEHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHC
TI
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]