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The map label for this gene is ybdL [H]

Identifier: 152969216

GI number: 152969216

Start: 733218

End: 734378

Strand: Direct

Name: ybdL [H]

Synonym: KPN_00645

Alternate gene names: 152969216

Gene position: 733218-734378 (Clockwise)

Preceding gene: 152969213

Following gene: 152969221

Centisome position: 13.79

GC content: 63.22

Gene sequence:

>1161_bases
ATGAACAATAACCCCTTGATTCCAGAGAGTAAACTTCCTGCGCTCGGCACCACGATTTTTACCCAGATGAGCGCCCTGGC
CCAGCAGCATCAGGCCATAAACCTGTCCCAGGGCTTTCCCGATTTTGACGGCCCGCGCTATCTTCAGGAGCGTCTGGCGT
ACCACGTGGCGCAGGGGGCGAATCAGTACGCCCCGATGACCGGCGTGCCGGCGCTGCGCGAGGCGATCGCCGGGAAGACG
GCGGAGCTGTATGGTTACCAGCCCGACGTTAACAGCGAGATCACGGTCACCGCCGGCGCGACGGAGGCCCTGTATGCGGC
GATCACCGCCCTGGTGCGCCGCGGCGACGAGGTGATCTGTTTCGATCCCAGCTATGACAGCTACGCCCCCGCGGTGGCGC
TGGCCGGCGGCGAGTTACGTCGTATTGCGCTGCAGCCGCCGCATTTTCGCGTCGACTGGCGGCAATTTGCCGCCGCCCTG
AGCGAGAAAACCCGGCTGGTGATCCTCAACACCCCGCACAACCCTTCGGCCACCGTCTGGCAGCGCGAGGATTTTGCCGC
CCTGTGGCAGGCGATCGCCGAACGCGAGATCTATGTCCTGAGCGATGAAGTCTATGAGCATATCTGCTTTGCTGAGGGCG
GCCACGCCAGCGTACTGGCGCATCCACAGCTGCGCGAGCGGGCGGTGGCGGTCTCTTCGTTTGGCAAAACCTTTCATATG
ACCGGCTGGAAGGTCGGCTACTGCGTGGCCCCGACGGCCATCAGCGCCGAACTGCGCAAGGTGCACCAGTATCTGACCTT
CTCGGTGAATACCCCGGCGCAGCTGGCGATCGCCGACATGCTGCGCGAAGCGCCGGAGCATTATCGTGAGCTGCCGGCGT
TTTATCGGGAACGTCGCGATCTGTTTATTGAAGCGCTGCGCCCAAGCCGGCTGGAAATCCTGCCCTGTGAAGGGACCTAT
TTCCTGCTGGCGGACTACAGCGCCATCTCCGATCTTGACGATGTCAGCTTCTGCCGCTGGTTGACCACTCAGATCGGCGT
GGCGGCGATCCCGCTCTCCGTCTTCTGCGCCGATCCGTTCCCGCACAAGCTGATCCGCTTGTGCTTCGCCAAACAGCCGG
CGACTCTGCTGGCTGCGGCGGCGCGTTTGTGCCAGCTGTAG

Upstream 100 bases:

>100_bases
GAATAACATTAGCATTTAGACGTCTAAGCGTCTTGATTGCCAAATATTAACATCGTGTTATAGTGGCTTCAACAACAGCA
TTACAATCGGTATAAAAAGA

Downstream 100 bases:

>100_bases
TTACTTAATCGTCCACGCCTGGGAGAACTGGCGGCCGGCGAACATTTCTGTCAGGCCGCTAATTTGCTTCAGACGCAGCA
CTTCATCGGCATCCATCCCC

Product: putative aminotransferase

Products: oxaloacetate; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MNNNPLIPESKLPALGTTIFTQMSALAQQHQAINLSQGFPDFDGPRYLQERLAYHVAQGANQYAPMTGVPALREAIAGKT
AELYGYQPDVNSEITVTAGATEALYAAITALVRRGDEVICFDPSYDSYAPAVALAGGELRRIALQPPHFRVDWRQFAAAL
SEKTRLVILNTPHNPSATVWQREDFAALWQAIAEREIYVLSDEVYEHICFAEGGHASVLAHPQLRERAVAVSSFGKTFHM
TGWKVGYCVAPTAISAELRKVHQYLTFSVNTPAQLAIADMLREAPEHYRELPAFYRERRDLFIEALRPSRLEILPCEGTY
FLLADYSAISDLDDVSFCRWLTTQIGVAAIPLSVFCADPFPHKLIRLCFAKQPATLLAAAARLCQL

Sequences:

>Translated_386_residues
MNNNPLIPESKLPALGTTIFTQMSALAQQHQAINLSQGFPDFDGPRYLQERLAYHVAQGANQYAPMTGVPALREAIAGKT
AELYGYQPDVNSEITVTAGATEALYAAITALVRRGDEVICFDPSYDSYAPAVALAGGELRRIALQPPHFRVDWRQFAAAL
SEKTRLVILNTPHNPSATVWQREDFAALWQAIAEREIYVLSDEVYEHICFAEGGHASVLAHPQLRERAVAVSSFGKTFHM
TGWKVGYCVAPTAISAELRKVHQYLTFSVNTPAQLAIADMLREAPEHYRELPAFYRERRDLFIEALRPSRLEILPCEGTY
FLLADYSAISDLDDVSFCRWLTTQIGVAAIPLSVFCADPFPHKLIRLCFAKQPATLLAAAARLCQL
>Mature_386_residues
MNNNPLIPESKLPALGTTIFTQMSALAQQHQAINLSQGFPDFDGPRYLQERLAYHVAQGANQYAPMTGVPALREAIAGKT
AELYGYQPDVNSEITVTAGATEALYAAITALVRRGDEVICFDPSYDSYAPAVALAGGELRRIALQPPHFRVDWRQFAAAL
SEKTRLVILNTPHNPSATVWQREDFAALWQAIAEREIYVLSDEVYEHICFAEGGHASVLAHPQLRERAVAVSSFGKTFHM
TGWKVGYCVAPTAISAELRKVHQYLTFSVNTPAQLAIADMLREAPEHYRELPAFYRERRDLFIEALRPSRLEILPCEGTY
FLLADYSAISDLDDVSFCRWLTTQIGVAAIPLSVFCADPFPHKLIRLCFAKQPATLLAAAARLCQL

Specific function: Shows aminotransferase activity with methionine and histidine as substrates, and to a lesser extent also with phenylalanine [H]

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI56713254, Length=399, Percent_Identity=33.5839598997494, Blast_Score=222, Evalue=4e-58,
Organism=Homo sapiens, GI56713256, Length=399, Percent_Identity=33.5839598997494, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI95147551, Length=408, Percent_Identity=31.6176470588235, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI169881279, Length=408, Percent_Identity=31.6176470588235, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI169881281, Length=406, Percent_Identity=27.0935960591133, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI4507369, Length=343, Percent_Identity=23.3236151603499, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1786816, Length=386, Percent_Identity=83.6787564766839, Blast_Score=678, Evalue=0.0,
Organism=Escherichia coli, GI1788722, Length=340, Percent_Identity=26.1764705882353, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1788627, Length=230, Percent_Identity=23.4782608695652, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71994476, Length=403, Percent_Identity=31.7617866004963, Blast_Score=211, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI71994472, Length=403, Percent_Identity=31.7617866004963, Blast_Score=211, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI17567369, Length=398, Percent_Identity=27.6381909547739, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17567663, Length=348, Percent_Identity=20.9770114942529, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6322401, Length=395, Percent_Identity=29.620253164557, Blast_Score=183, Evalue=4e-47,
Organism=Drosophila melanogaster, GI28573069, Length=401, Percent_Identity=32.4189526184539, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24646114, Length=401, Percent_Identity=32.4189526184539, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI28573067, Length=401, Percent_Identity=32.4189526184539, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI28573065, Length=401, Percent_Identity=32.4189526184539, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI18859735, Length=241, Percent_Identity=28.6307053941909, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: 2.6.1.1

Molecular weight: Translated: 42827; Mature: 42827

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNNPLIPESKLPALGTTIFTQMSALAQQHQAINLSQGFPDFDGPRYLQERLAYHVAQGA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHCC
NQYAPMTGVPALREAIAGKTAELYGYQPDVNSEITVTAGATEALYAAITALVRRGDEVIC
CCCCCCCCCHHHHHHHCCCCHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEE
FDPSYDSYAPAVALAGGELRRIALQPPHFRVDWRQFAAALSEKTRLVILNTPHNPSATVW
ECCCCCCCCCEEEECCCCEEEEEECCCCCEECHHHHHHHHCCCCEEEEEECCCCCCCCEE
QREDFAALWQAIAEREIYVLSDEVYEHICFAEGGHASVLAHPQLRERAVAVSSFGKTFHM
ECHHHHHHHHHHHCCCEEEECHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCEEEE
TGWKVGYCVAPTAISAELRKVHQYLTFSVNTPAQLAIADMLREAPEHYRELPAFYRERRD
CCCEEEEEECCHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFIEALRPSRLEILPCEGTYFLLADYSAISDLDDVSFCRWLTTQIGVAAIPLSVFCADPF
HHHHHCCCCCEEEEECCCCEEEEECCHHHCCCHHHHHHHHHHHHHCHHHHCCEEEECCCC
PHKLIRLCFAKQPATLLAAAARLCQL
HHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNNNPLIPESKLPALGTTIFTQMSALAQQHQAINLSQGFPDFDGPRYLQERLAYHVAQGA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHCC
NQYAPMTGVPALREAIAGKTAELYGYQPDVNSEITVTAGATEALYAAITALVRRGDEVIC
CCCCCCCCCHHHHHHHCCCCHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEE
FDPSYDSYAPAVALAGGELRRIALQPPHFRVDWRQFAAALSEKTRLVILNTPHNPSATVW
ECCCCCCCCCEEEECCCCEEEEEECCCCCEECHHHHHHHHCCCCEEEEEECCCCCCCCEE
QREDFAALWQAIAEREIYVLSDEVYEHICFAEGGHASVLAHPQLRERAVAVSSFGKTFHM
ECHHHHHHHHHHHCCCEEEECHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCEEEE
TGWKVGYCVAPTAISAELRKVHQYLTFSVNTPAQLAIADMLREAPEHYRELPAFYRERRD
CCCEEEEEECCHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFIEALRPSRLEILPCEGTYFLLADYSAISDLDDVSFCRWLTTQIGVAAIPLSVFCADPF
HHHHHCCCCCEEEEECCCCEEEEECCHHHCCCHHHHHHHHHHHHHCHHHHCCEEEECCCC
PHKLIRLCFAKQPATLLAAAARLCQL
HHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate; 2-oxoglutarate

Specific reaction: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]