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The map label for this gene is entB [H]

Identifier: 152969184

GI number: 152969184

Start: 695288

End: 696139

Strand: Direct

Name: entB [H]

Synonym: KPN_00613

Alternate gene names: 152969184

Gene position: 695288-696139 (Clockwise)

Preceding gene: 152969183

Following gene: 152969185

Centisome position: 13.08

GC content: 60.56

Gene sequence:

>852_bases
ATGGCAATCCCTAAATTACAGGCATATGCGCTGCCGGAAGCCAGCGATATCCCGGCGAACAAGGTCAACTGGGCCTTTGA
GCCGTCGCGCGCCGCGCTGCTGATCCACGATATGCAGGAATATTTCCTCAACTTCTGGGGCGAAAACAGCGCGATGATGG
AGAAAGTGGTGGCTAATATCGCCGCCCTGCGCGACTTCTGCAAACAGAACGGCATTCCGGTGTACTACACCGCCCAGCCG
AAAGAGCAGAGCGATGAAGACCGCGCCCTGCTGAATGATATGTGGGGGCCGGGTCTGACCCGCTCGCCGGAGCAGCAGCA
GGTGATTGCCGCGCTGGCGCCGGATGAAGACGATACCGTGCTGGTGAAATGGCGCTACAGCGCGTTTCATCGCTCGCCGC
TGGAGGAGATGCTGAAAGAGACCGGTCGCGACCAGCTGATAATCACCGGCGTTTACGCCCATATCGGCTGCATGACCACC
GCCACCGATGCTTTCATGCGCGATATCAAACCGTTCTTTGTCGCCGACGCGCTGGCAGATTTCAGCCGTGAAGAGCACCT
GATGGCGCTGAAATACGTCGCTGGCCGCTCCGGCCGCGTGGTGATGACCGAAGAGCTGCTGCCGCTGCCGGCCTCCAAAG
CGGCGCTGCGCGCGCTGATCCTGCCGCTGCTCGACGAATCCGACGAACCGCTGGATGATGAAAATCTGATCGACTACGGT
CTGGATTCGGTGCGGATGATGGCCCTGGCCGCCCGCTGGCGCAAAGTGCACGGCGATATCGACTTCGTGATGCTGGCGAA
GAACCCGACCATCGACGCCTGGTGGGCGCTGCTCTCCCGTGAGGTGAAATAA

Upstream 100 bases:

>100_bases
TGCGTTGCCGCGCTGCCGCTGACGCCGGTGGGCAAAGTCGATAAAAAACAATTACGTCAGTGGCTGGCCGAAGGCAAGCT
GGGCTGAAGGAGAACAGAGA

Downstream 100 bases:

>100_bases
TGGCGGCGCTGGATTTCCACGGTCAGACCGTGTGGGTGACCGGCGCAGGTAAAGGGATTGGCTATGCCACCGCGCTGGCT
TTCGTCGAGGCCGGGGCCAA

Product: 2,3-dihydro-2,3-dihydroxybenzoate synthetase, isochroismatase

Products: NA

Alternate protein names: 2,3 dihydro-2,3 dihydroxybenzoate synthase; Enterobactin synthase component B; Enterochelin synthase B; Isochorismate lyase [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MAIPKLQAYALPEASDIPANKVNWAFEPSRAALLIHDMQEYFLNFWGENSAMMEKVVANIAALRDFCKQNGIPVYYTAQP
KEQSDEDRALLNDMWGPGLTRSPEQQQVIAALAPDEDDTVLVKWRYSAFHRSPLEEMLKETGRDQLIITGVYAHIGCMTT
ATDAFMRDIKPFFVADALADFSREEHLMALKYVAGRSGRVVMTEELLPLPASKAALRALILPLLDESDEPLDDENLIDYG
LDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAWWALLSREVK

Sequences:

>Translated_283_residues
MAIPKLQAYALPEASDIPANKVNWAFEPSRAALLIHDMQEYFLNFWGENSAMMEKVVANIAALRDFCKQNGIPVYYTAQP
KEQSDEDRALLNDMWGPGLTRSPEQQQVIAALAPDEDDTVLVKWRYSAFHRSPLEEMLKETGRDQLIITGVYAHIGCMTT
ATDAFMRDIKPFFVADALADFSREEHLMALKYVAGRSGRVVMTEELLPLPASKAALRALILPLLDESDEPLDDENLIDYG
LDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAWWALLSREVK
>Mature_282_residues
AIPKLQAYALPEASDIPANKVNWAFEPSRAALLIHDMQEYFLNFWGENSAMMEKVVANIAALRDFCKQNGIPVYYTAQPK
EQSDEDRALLNDMWGPGLTRSPEQQQVIAALAPDEDDTVLVKWRYSAFHRSPLEEMLKETGRDQLIITGVYAHIGCMTTA
TDAFMRDIKPFFVADALADFSREEHLMALKYVAGRSGRVVMTEELLPLPASKAALRALILPLLDESDEPLDDENLIDYGL
DSVRMMALAARWRKVHGDIDFVMLAKNPTIDAWWALLSREVK

Specific function: Required for production of 2,3-DHB. Also serves as an aryl carrier protein and plays a role in enterobactin assembly [H]

COG id: COG1535

COG function: function code Q; Isochorismate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

Organism=Escherichia coli, GI1786811, Length=285, Percent_Identity=87.719298245614, Blast_Score=524, Evalue=1e-150,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009081
- InterPro:   IPR016291
- InterPro:   IPR000868
- InterPro:   IPR006163 [H]

Pfam domain/function: PF00857 Isochorismatase; PF00550 PP-binding [H]

EC number: =3.3.2.1 [H]

Molecular weight: Translated: 31946; Mature: 31815

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS50075 ACP_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPKLQAYALPEASDIPANKVNWAFEPSRAALLIHDMQEYFLNFWGENSAMMEKVVANI
CCCCCCHHEECCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AALRDFCKQNGIPVYYTAQPKEQSDEDRALLNDMWGPGLTRSPEQQQVIAALAPDEDDTV
HHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCHHHEEEEECCCCCCEE
LVKWRYSAFHRSPLEEMLKETGRDQLIITGVYAHIGCMTTATDAFMRDIKPFFVADALAD
EEEEEHHHHCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
FSREEHLMALKYVAGRSGRVVMTEELLPLPASKAALRALILPLLDESDEPLDDENLIDYG
HCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHC
LDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAWWALLSREVK
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AIPKLQAYALPEASDIPANKVNWAFEPSRAALLIHDMQEYFLNFWGENSAMMEKVVANI
CCCCCHHEECCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AALRDFCKQNGIPVYYTAQPKEQSDEDRALLNDMWGPGLTRSPEQQQVIAALAPDEDDTV
HHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCHHHEEEEECCCCCCEE
LVKWRYSAFHRSPLEEMLKETGRDQLIITGVYAHIGCMTTATDAFMRDIKPFFVADALAD
EEEEEHHHHCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
FSREEHLMALKYVAGRSGRVVMTEELLPLPASKAALRALILPLLDESDEPLDDENLIDYG
HCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHC
LDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAWWALLSREVK
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]