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The map label for this gene is cycB [H]

Identifier: 152969000

GI number: 152969000

Start: 469698

End: 470930

Strand: Direct

Name: cycB [H]

Synonym: KPN_00427

Alternate gene names: 152969000

Gene position: 469698-470930 (Clockwise)

Preceding gene: 152968997

Following gene: 152969001

Centisome position: 8.84

GC content: 59.04

Gene sequence:

>1233_bases
ATGAAAAAGAACACGCTTGCTGCACTGATCCTTACCACCCTGGCGGCGGGGCAACTCGCCAGCCTGCAGGCACATGCCGC
CGGGCAGCTCAACGTCTGGGAGGACATTAAAAAATCCGCTGGCATCAAAACCGCGGTTAGCGATTTTGAAAAGCAGTACA
ACGTGAAGGTCAATCTCCAGGAGATGCCCTACGCCCAGCAACTGGAAAAACTGCGCCTCGACGGCCCGGCGGGCATTGGG
CCGGACGTGCTGGTGATCCCGAATGACCAGCTCGGCGGCGCCGTGGTTCAGGGACTACTCTCGCCGCTCAGCGTCGATCA
GGCGAAACAGGATGCCTTTACGCCAGCCTCCATCAATGCCTTCCGCATGGACAACGCGCTGTATGGGATCCCGAAGGCGG
TGGAAACCCTGGTGCTTATCTACAACAAGGACCTGATCGACAAGCCGCTGGACAGCCTGCAGGCCTGGCTGGACTACTCG
AAAACCCAGCGCGAGCAGAACAAATACGGCCTGCTCGCCAAGTTTGACCAGATCTATTACAGCTGGGGGGCGATTGGCCC
GATGGGCGGCTATATCTTTGCTAAAAACGACAGCGGCGGCTTTAACCCGCAGCAGGTGGGTCTCAATACCCCCGGCGCCG
TCGAAGCCGTCACCTTCCTGAAAAAATTCTATGCCGAGAAGGTCTTTCCGGCGGGGATCCTCGGCGATAACGGGCTGAAT
GCCATCGATTCCCTGTTTACCGAGAAAAAAGCGGCGGCGGTGATCAACGGCCCCTGGGCCTTCCAGCCGTATGAAGCCGC
TGGCATTAACTATGGCGTGGCGCCCCTGCCGACCCTGCCGGACGGCAAGCCGATGAGCTCCTTCCTCGGCGTCAAAGGCT
ATGTAGTATCGACCTGGAGTAAAGACAAAGCGCTGGCGCAACAGTTTATCGAGTTCATCAACCAGCCGCAGTATGTGAAA
GCCCGCTATGTCGCCACTGGCGAAATTCCGCCGCTGAAGGCGATGATTGACGATCCGGTGATTAAAAACGACCAAAAGGC
CAGCGCCGTCGCCATCCAGTCGGCGCGAGCGGTAGCGATGCCGGGCATTCCCGAGATGGGGGAAGTGTGGGGGCCGGCCA
ACGCCGCGCTTGAACTGAGCCTCACCGGTAAGCAGGCGCCGCAGGCCGCGCTTGATAACGCGGTGAAACAGATCACGATG
CAGATCGAAGCGATGCAGGCCAGCAATCAGTAA

Upstream 100 bases:

>100_bases
TCCACATAATACGGCAGTTTGAAAAGATTTATTTTTCTAACTGGCAGACGGAATTCAAATAAAAACCATTAAATTGTTAT
ACCTTCACATGAGGTTGATA

Downstream 100 bases:

>100_bases
TTCACCGTCACGTGAAGTCACCGAGCGGGAGGGGGACCTCCCGTTTGTAGAAGGAGTCGAGTGTGAGCATCCATTCCAGC
GAAAATTTTAGCGACGCACG

Product: periplasmic maltose-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKKNTLAALILTTLAAGQLASLQAHAAGQLNVWEDIKKSAGIKTAVSDFEKQYNVKVNLQEMPYAQQLEKLRLDGPAGIG
PDVLVIPNDQLGGAVVQGLLSPLSVDQAKQDAFTPASINAFRMDNALYGIPKAVETLVLIYNKDLIDKPLDSLQAWLDYS
KTQREQNKYGLLAKFDQIYYSWGAIGPMGGYIFAKNDSGGFNPQQVGLNTPGAVEAVTFLKKFYAEKVFPAGILGDNGLN
AIDSLFTEKKAAAVINGPWAFQPYEAAGINYGVAPLPTLPDGKPMSSFLGVKGYVVSTWSKDKALAQQFIEFINQPQYVK
ARYVATGEIPPLKAMIDDPVIKNDQKASAVAIQSARAVAMPGIPEMGEVWGPANAALELSLTGKQAPQAALDNAVKQITM
QIEAMQASNQ

Sequences:

>Translated_410_residues
MKKNTLAALILTTLAAGQLASLQAHAAGQLNVWEDIKKSAGIKTAVSDFEKQYNVKVNLQEMPYAQQLEKLRLDGPAGIG
PDVLVIPNDQLGGAVVQGLLSPLSVDQAKQDAFTPASINAFRMDNALYGIPKAVETLVLIYNKDLIDKPLDSLQAWLDYS
KTQREQNKYGLLAKFDQIYYSWGAIGPMGGYIFAKNDSGGFNPQQVGLNTPGAVEAVTFLKKFYAEKVFPAGILGDNGLN
AIDSLFTEKKAAAVINGPWAFQPYEAAGINYGVAPLPTLPDGKPMSSFLGVKGYVVSTWSKDKALAQQFIEFINQPQYVK
ARYVATGEIPPLKAMIDDPVIKNDQKASAVAIQSARAVAMPGIPEMGEVWGPANAALELSLTGKQAPQAALDNAVKQITM
QIEAMQASNQ
>Mature_410_residues
MKKNTLAALILTTLAAGQLASLQAHAAGQLNVWEDIKKSAGIKTAVSDFEKQYNVKVNLQEMPYAQQLEKLRLDGPAGIG
PDVLVIPNDQLGGAVVQGLLSPLSVDQAKQDAFTPASINAFRMDNALYGIPKAVETLVLIYNKDLIDKPLDSLQAWLDYS
KTQREQNKYGLLAKFDQIYYSWGAIGPMGGYIFAKNDSGGFNPQQVGLNTPGAVEAVTFLKKFYAEKVFPAGILGDNGLN
AIDSLFTEKKAAAVINGPWAFQPYEAAGINYGVAPLPTLPDGKPMSSFLGVKGYVVSTWSKDKALAQQFIEFINQPQYVK
ARYVATGEIPPLKAMIDDPVIKNDQKASAVAIQSARAVAMPGIPEMGEVWGPANAALELSLTGKQAPQAALDNAVKQITM
QIEAMQASNQ

Specific function: Binding protein for cyclodextrin; involved in its cellular uptake. Interacts with all natural cyclodextrins:alpha, beta and gamma [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=399, Percent_Identity=29.3233082706767, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 44092; Mature: 44092

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNTLAALILTTLAAGQLASLQAHAAGQLNVWEDIKKSAGIKTAVSDFEKQYNVKVNLQ
CCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHCCEEEEHH
EMPYAQQLEKLRLDGPAGIGPDVLVIPNDQLGGAVVQGLLSPLSVDQAKQDAFTPASINA
HCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCCEE
FRMDNALYGIPKAVETLVLIYNKDLIDKPLDSLQAWLDYSKTQREQNKYGLLAKFDQIYY
EEECCCHHCCHHHHHHEEHEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SWGAIGPMGGYIFAKNDSGGFNPQQVGLNTPGAVEAVTFLKKFYAEKVFPAGILGDNGLN
HHCCCCCCCCEEEEECCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCHH
AIDSLFTEKKAAAVINGPWAFQPYEAAGINYGVAPLPTLPDGKPMSSFLGVKGYVVSTWS
HHHHHHHHHHHHEEECCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEECCC
KDKALAQQFIEFINQPQYVKARYVATGEIPPLKAMIDDPVIKNDQKASAVAIQSARAVAM
HHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHCCCCCCCCCCHHHHHHHCCCEEEC
PGIPEMGEVWGPANAALELSLTGKQAPQAALDNAVKQITMQIEAMQASNQ
CCCCCHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKNTLAALILTTLAAGQLASLQAHAAGQLNVWEDIKKSAGIKTAVSDFEKQYNVKVNLQ
CCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHCCEEEEHH
EMPYAQQLEKLRLDGPAGIGPDVLVIPNDQLGGAVVQGLLSPLSVDQAKQDAFTPASINA
HCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCCEE
FRMDNALYGIPKAVETLVLIYNKDLIDKPLDSLQAWLDYSKTQREQNKYGLLAKFDQIYY
EEECCCHHCCHHHHHHEEHEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SWGAIGPMGGYIFAKNDSGGFNPQQVGLNTPGAVEAVTFLKKFYAEKVFPAGILGDNGLN
HHCCCCCCCCEEEEECCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCHH
AIDSLFTEKKAAAVINGPWAFQPYEAAGINYGVAPLPTLPDGKPMSSFLGVKGYVVSTWS
HHHHHHHHHHHHEEECCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEECCC
KDKALAQQFIEFINQPQYVKARYVATGEIPPLKAMIDDPVIKNDQKASAVAIQSARAVAM
HHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHCCCCCCCCCCHHHHHHHCCCEEEC
PGIPEMGEVWGPANAALELSLTGKQAPQAALDNAVKQITMQIEAMQASNQ
CCCCCHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]