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The map label for this gene is pcaR [H]

Identifier: 152968961

GI number: 152968961

Start: 428942

End: 429700

Strand: Direct

Name: pcaR [H]

Synonym: KPN_00388

Alternate gene names: 152968961

Gene position: 428942-429700 (Clockwise)

Preceding gene: 152968952

Following gene: 152968970

Centisome position: 8.07

GC content: 59.42

Gene sequence:

>759_bases
ATGCAAAACACCATTCATCCACGCGATCTGATCGTCGGTTTACAAAAAGGGCTGGCGCTAATTCAGCTTTTTTCCAAAAC
CTGCCCAAAGCTCACCGTCGCCCAGACGGCGAAAATGAGCGGCCTGACGCAAAGCGCCGCGCGACGTTTTCTGCTGACGC
TCCTGCATGAGCGCTATCTGCAAACCGACGGGCGCTATTACTGGCTTACCCCAAAGACGCTGCGGCTTGGCCAGGCCTAT
GTCGATTCGGCGCAGTTTCCTCGCATGGTACGGCCGATTGTCGAATACATCGCCAGCCGGACCGAAGAGCACGCCTCGGT
GGGGGTGGTGGATGAAGATGAACTGGTCTATATCGCCCGCAGTCGGCATACGCCGTTCAACTCTACGTCGGTGCGGCTTG
GGGAGCGGGTGCCCATTTTCTGTACCGCCGGGGGGCGACTGTGGCTGGCTTCGCTGCCGGAAGCAGAGTGTGAGACCGTC
CTGCAGCGCATAACCCGGGAACAACGTACCCCCTATACGGTGACCGATATCGCGTCGCTGATGGAGAAAATCGCCCAGGT
ACGGCGCCAGGGATACGCCACCATTGAGCAGGAGTTTGAGATTGGCATGCTGGTGCTGGCGGTGCCGTTAACCGACAGGG
AAGGGACCTGGTGGGGGGCGCTGAGTCTGACCAGTCATCAATCGCGCACTTCGCTGGAAGCGTTATGCCGCGATCACCTC
GATCTGCTCTACAGCGCGCAGGCGATGCTGGTGGGTTAA

Upstream 100 bases:

>100_bases
GGAGCGACAACGCAGAATTCGGACATGTCGGTTGTTAATCGCACGAACGCTGCGATAATCGCAGGAAATCGATAATGATG
TTGCAAAAATGTGAGCGAAT

Downstream 100 bases:

>100_bases
ACAGGGCAGCCAGTGGCGCTGCCCTAAGATAGACGTCGCTTAGGCCGACGCTTTGGTCAGAAAGGTGTTCCAGTCGCTGG
CCGCGTTATTCACGAAAGTA

Product: regulatory proteins, IclR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MQNTIHPRDLIVGLQKGLALIQLFSKTCPKLTVAQTAKMSGLTQSAARRFLLTLLHERYLQTDGRYYWLTPKTLRLGQAY
VDSAQFPRMVRPIVEYIASRTEEHASVGVVDEDELVYIARSRHTPFNSTSVRLGERVPIFCTAGGRLWLASLPEAECETV
LQRITREQRTPYTVTDIASLMEKIAQVRRQGYATIEQEFEIGMLVLAVPLTDREGTWWGALSLTSHQSRTSLEALCRDHL
DLLYSAQAMLVG

Sequences:

>Translated_252_residues
MQNTIHPRDLIVGLQKGLALIQLFSKTCPKLTVAQTAKMSGLTQSAARRFLLTLLHERYLQTDGRYYWLTPKTLRLGQAY
VDSAQFPRMVRPIVEYIASRTEEHASVGVVDEDELVYIARSRHTPFNSTSVRLGERVPIFCTAGGRLWLASLPEAECETV
LQRITREQRTPYTVTDIASLMEKIAQVRRQGYATIEQEFEIGMLVLAVPLTDREGTWWGALSLTSHQSRTSLEALCRDHL
DLLYSAQAMLVG
>Mature_252_residues
MQNTIHPRDLIVGLQKGLALIQLFSKTCPKLTVAQTAKMSGLTQSAARRFLLTLLHERYLQTDGRYYWLTPKTLRLGQAY
VDSAQFPRMVRPIVEYIASRTEEHASVGVVDEDELVYIARSRHTPFNSTSVRLGERVPIFCTAGGRLWLASLPEAECETV
LQRITREQRTPYTVTDIASLMEKIAQVRRQGYATIEQEFEIGMLVLAVPLTDREGTWWGALSLTSHQSRTSLEALCRDHL
DLLYSAQAMLVG

Specific function: Positive regulator of all genes within the pca regulon, pcaBDC, pcaIJ and pcaF. Also required for the chemotactic response to aromatic compounds [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=223, Percent_Identity=29.5964125560538, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788131, Length=207, Percent_Identity=29.4685990338164, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87082359, Length=222, Percent_Identity=22.972972972973, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786715, Length=218, Percent_Identity=22.9357798165138, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789999, Length=226, Percent_Identity=25.6637168141593, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 28482; Mature: 28482

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNTIHPRDLIVGLQKGLALIQLFSKTCPKLTVAQTAKMSGLTQSAARRFLLTLLHERYL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTDGRYYWLTPKTLRLGQAYVDSAQFPRMVRPIVEYIASRTEEHASVGVVDEDELVYIAR
HCCCCEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEEE
SRHTPFNSTSVRLGERVPIFCTAGGRLWLASLPEAECETVLQRITREQRTPYTVTDIASL
CCCCCCCCCCEECCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
MEKIAQVRRQGYATIEQEFEIGMLVLAVPLTDREGTWWGALSLTSHQSRTSLEALCRDHL
HHHHHHHHHCCCHHHHHHHCCCEEEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHH
DLLYSAQAMLVG
HHHHHHHHHCCC
>Mature Secondary Structure
MQNTIHPRDLIVGLQKGLALIQLFSKTCPKLTVAQTAKMSGLTQSAARRFLLTLLHERYL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTDGRYYWLTPKTLRLGQAYVDSAQFPRMVRPIVEYIASRTEEHASVGVVDEDELVYIAR
HCCCCEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCEEEEEE
SRHTPFNSTSVRLGERVPIFCTAGGRLWLASLPEAECETVLQRITREQRTPYTVTDIASL
CCCCCCCCCCEECCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
MEKIAQVRRQGYATIEQEFEIGMLVLAVPLTDREGTWWGALSLTSHQSRTSLEALCRDHL
HHHHHHHHHCCCHHHHHHHCCCEEEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHH
DLLYSAQAMLVG
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8083169 [H]