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The map label for this gene is yajF [H]

Identifier: 152968910

GI number: 152968910

Start: 374516

End: 375463

Strand: Direct

Name: yajF [H]

Synonym: KPN_00337

Alternate gene names: 152968910

Gene position: 374516-375463 (Clockwise)

Preceding gene: 152968908

Following gene: 152968913

Centisome position: 7.05

GC content: 61.92

Gene sequence:

>948_bases
ATGCTTGCATTGTTAATCGGTGAGGAGCTGAGCGTGCGTATTGGTATTGATTTAGGTGGAACAAAAACCGAAGTGATAGC
CCTGAGCGACCAGGGAGAGCAGCTGTTTCGCCACCGGTTACCCACGCCGCGGCAGGACTACCGGCAAACCATCGAGACCA
TCGCCACTCTGGTGGCGATGGCCGAGCAGGCGACCGGACAGCAGGGTACCGTGGGGATGGGCATTCCTGGCTCAATTTCG
CCTTATACCGGGGTGGTGAAAAACGCCAACTCGACCTGGCTCAACGGCCAGCCCTTTGATAAGGACCTCAGCCTGCGCCT
TGAGCGGGAAGTCCGGCTGGCTAACGATGCCAACTGTCTGGCGGTGTCGGAAGCGGTGGACGGCGCGGCGGCGGGGGCGC
AAACGGTATTCGCGGTGATTATCGGCACCGGCTGCGGCGCCGGGGTGGCGCTCAACGGGCGGGCGCATATCGGCGGCAAT
GGTAACGCTGGCGAATGGGGACATAACCCATTGCCGTGGATGAACGACGATGAGCTGCGCTACCGTGCGGAAGTCCCTTG
CTACTGCGGCAAACAGGGGTGTATCGAAACCTTTATCTCCGGCACCGGTTTCGCCACCGACTATCAGCGGCTGAGCGGCA
AAGGGTTAACCGGCAGCGAGATTATGCGCCTGGTGGGAGAAGGTGACGAAAAGGCTGAACTGGCGCTTAGCCGCTATGAA
CAGCGGCTGGCGAAATCGCTGGCCCACGTGGTGAATATCCTCGACCCGGACGTTATCGTGCTGGGCGGCGGGATGAGCAA
CGTCGAACGACTGTATCAGACGGTGCCGGACCTGGTGAAGCAGTGGGTCTTCGGCGGCGAGTGTGAAACGCCGATCCGCA
AAGCGATGCACGGCGACTCCAGCGGGGTACGCGGCGCGGCCTGGCTGTGGCCGCTCCAGGGGACCTGA

Upstream 100 bases:

>100_bases
TCGCGGCTGAGACGGTAAACCATTAAATTTTTGAACCACAGCATGATAATTTCCACGGCTTTGTCGTTAAATCAGGCGGG
CATGATAGCGAATTGTCGCT

Downstream 100 bases:

>100_bases
ACCGCTTCCCCGCACCCGGATCCTCTCCCTCGGGAGAGGGTTAGGGTGAGGGAACATCGCCATAATTCAGACCTCACTCC
ACCGCAAACGCTTTGTCCAG

Product: NAGC-like transcriptional regulator

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MLALLIGEELSVRIGIDLGGTKTEVIALSDQGEQLFRHRLPTPRQDYRQTIETIATLVAMAEQATGQQGTVGMGIPGSIS
PYTGVVKNANSTWLNGQPFDKDLSLRLEREVRLANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGN
GNAGEWGHNPLPWMNDDELRYRAEVPCYCGKQGCIETFISGTGFATDYQRLSGKGLTGSEIMRLVGEGDEKAELALSRYE
QRLAKSLAHVVNILDPDVIVLGGGMSNVERLYQTVPDLVKQWVFGGECETPIRKAMHGDSSGVRGAAWLWPLQGT

Sequences:

>Translated_315_residues
MLALLIGEELSVRIGIDLGGTKTEVIALSDQGEQLFRHRLPTPRQDYRQTIETIATLVAMAEQATGQQGTVGMGIPGSIS
PYTGVVKNANSTWLNGQPFDKDLSLRLEREVRLANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGN
GNAGEWGHNPLPWMNDDELRYRAEVPCYCGKQGCIETFISGTGFATDYQRLSGKGLTGSEIMRLVGEGDEKAELALSRYE
QRLAKSLAHVVNILDPDVIVLGGGMSNVERLYQTVPDLVKQWVFGGECETPIRKAMHGDSSGVRGAAWLWPLQGT
>Mature_315_residues
MLALLIGEELSVRIGIDLGGTKTEVIALSDQGEQLFRHRLPTPRQDYRQTIETIATLVAMAEQATGQQGTVGMGIPGSIS
PYTGVVKNANSTWLNGQPFDKDLSLRLEREVRLANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGN
GNAGEWGHNPLPWMNDDELRYRAEVPCYCGKQGCIETFISGTGFATDYQRLSGKGLTGSEIMRLVGEGDEKAELALSRYE
QRLAKSLAHVVNILDPDVIVLGGGMSNVERLYQTVPDLVKQWVFGGECETPIRKAMHGDSSGVRGAAWLWPLQGT

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=300, Percent_Identity=89, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI1787363, Length=299, Percent_Identity=40.133779264214, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI1786891, Length=272, Percent_Identity=23.1617647058824, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790522, Length=319, Percent_Identity=26.9592476489028, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787878, Length=232, Percent_Identity=25.8620689655172, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 33640; Mature: 33640

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLALLIGEELSVRIGIDLGGTKTEVIALSDQGEQLFRHRLPTPRQDYRQTIETIATLVAM
CEEEEECCCEEEEEEEECCCCCEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
AEQATGQQGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSLRLEREVRLANDANCL
HHHHCCCCCCEECCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHEEEEEHEEECCCCCEE
AVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGNAGEWGHNPLPWMNDDELR
EEHHHHCCHHHHHHHEEEEEEECCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCCCEE
YRAEVPCYCGKQGCIETFISGTGFATDYQRLSGKGLTGSEIMRLVGEGDEKAELALSRYE
EEECCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHH
QRLAKSLAHVVNILDPDVIVLGGGMSNVERLYQTVPDLVKQWVFGGECETPIRKAMHGDS
HHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
SGVRGAAWLWPLQGT
CCCCCEEEEEECCCC
>Mature Secondary Structure
MLALLIGEELSVRIGIDLGGTKTEVIALSDQGEQLFRHRLPTPRQDYRQTIETIATLVAM
CEEEEECCCEEEEEEEECCCCCEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
AEQATGQQGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSLRLEREVRLANDANCL
HHHHCCCCCCEECCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHEEEEEHEEECCCCCEE
AVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGNAGEWGHNPLPWMNDDELR
EEHHHHCCHHHHHHHEEEEEEECCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCCCEE
YRAEVPCYCGKQGCIETFISGTGFATDYQRLSGKGLTGSEIMRLVGEGDEKAELALSRYE
EEECCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHH
QRLAKSLAHVVNILDPDVIVLGGGMSNVERLYQTVPDLVKQWVFGGECETPIRKAMHGDS
HHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
SGVRGAAWLWPLQGT
CCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]