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The map label for this gene is sbmA [H]

Identifier: 152968892

GI number: 152968892

Start: 360021

End: 361244

Strand: Direct

Name: sbmA [H]

Synonym: KPN_00319

Alternate gene names: 152968892

Gene position: 360021-361244 (Clockwise)

Preceding gene: 152968891

Following gene: 152968893

Centisome position: 6.77

GC content: 56.94

Gene sequence:

>1224_bases
ATGATGTTTAAATCTTTTTTCCCCAAACCGGGGCCGTTTTTTATTTCTGCCTTTATCTGGTCGCTGCTGGCCGTGATCTT
TTGGCAGGCGGGCGGCGGCGACTGGCTGCTGCGGGTCACCGGGGCATCGCAAAATGTGGCGATCAGCGCGGCGCGCTTCT
GGTCGCTCAACTATCTGGTGTTCTACGCTTACTATCTGTTTTGCGTCGGCGTGTTCGCGCTGTTCTGGTTTGTCTACTGC
CCGCACCGCTGGCAGTACTGGTCGATTCTCGGCACCTCGCTCATCATCTTCGTCACCTGGTTTCTGGTGGAAGTCGGGGT
GGCGATCAACGCCTGGTATGCGCCGTTCTACGACCTGATCCAGAGCGCGCTGGCCACCCCGCATAAGGTCAGCATCAACC
AGTTTTATCAGGAAATCGGCGTCTTTCTCGGCATCGCGATCATCGCGGTGATTATTGGCGTGATGAACAACTTCTTCGTC
AGCCACTACGTCTTCCGCTGGCGCACGGCGATGAACGAGCACTATATGGCCCACTGGCAGCATCTGCGCCATATCGAAGG
CGCCGCGCAGCGTGTGCAGGAAGATACCATGCGTTTTGCTTCCACCCTCGAAGATATGGGCGTGAGCTTTATCAACGCGG
TGATGACCCTGATCGCCTTCCTGCCGGTGCTGGTGACGCTCTCCGAACATGTTCCCGACCTGCCTATCGTCGGCCATCTG
CCGTATGGCCTGGTGATTGCCGCTATCGTCTGGTCGCTGATGGGTACCGGCCTGCTGGCGGTGGTGGGGATTAAGCTGCC
GGGCCTGGAGTTTAAAAACCAGCGCGTGGAAGCGGCCTATCGTAAAGAGCTGGTTTACGGCGAGGACGATGAAACTCGCG
CCACGCCGCCGACGGTGCGCGAGCTATTCCGCGCGGTGCGTCGCAACTATTTCCGTCTCTATTTCCACTATATGTATTTC
AATATCGCCCGCATCCTCTATCTGCAGGTGGATAACGTTTTCGGCCTTTTCCTGCTGTTTCCGTCGATCGTTGCCGGTAC
GATTACCCTCGGGCTGATGACGCAAATTACCAACGTCTTCGGCCAGGTGCGCGGCTCGTTCCAGTACCTGATCAGCTCGT
GGACAACGCTGGTGGAACTGATGTCCATCTACAAACGTCTGCGCAGTTTTGAACGCGAGCTGGACGGCAAACCGCTGCAG
GAAGCGATCCCGACATTACGTTAA

Upstream 100 bases:

>100_bases
GTCTTATCATTCGTCTGTCCCGCGAGGGCTCATCCGCGCGGCGGCCGACGTATGCGTCGTACACAGCAATAGCAGAACGA
TAAGAAGTTAGCAGGAGACA

Downstream 100 bases:

>100_bases
TCAAGACAAGGAAGCATAATGGCCGCACGTTTATCGCGCATTATTCCCGTTTCATTGACGCTGCTGGCGGCCATGGCGCT
GAGCGCCTGTACCAGCCAGC

Product: transport protein

Products: ADP; phosphate; microcin B17 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MMFKSFFPKPGPFFISAFIWSLLAVIFWQAGGGDWLLRVTGASQNVAISAARFWSLNYLVFYAYYLFCVGVFALFWFVYC
PHRWQYWSILGTSLIIFVTWFLVEVGVAINAWYAPFYDLIQSALATPHKVSINQFYQEIGVFLGIAIIAVIIGVMNNFFV
SHYVFRWRTAMNEHYMAHWQHLRHIEGAAQRVQEDTMRFASTLEDMGVSFINAVMTLIAFLPVLVTLSEHVPDLPIVGHL
PYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVEAAYRKELVYGEDDETRATPPTVRELFRAVRRNYFRLYFHYMYF
NIARILYLQVDNVFGLFLLFPSIVAGTITLGLMTQITNVFGQVRGSFQYLISSWTTLVELMSIYKRLRSFERELDGKPLQ
EAIPTLR

Sequences:

>Translated_407_residues
MMFKSFFPKPGPFFISAFIWSLLAVIFWQAGGGDWLLRVTGASQNVAISAARFWSLNYLVFYAYYLFCVGVFALFWFVYC
PHRWQYWSILGTSLIIFVTWFLVEVGVAINAWYAPFYDLIQSALATPHKVSINQFYQEIGVFLGIAIIAVIIGVMNNFFV
SHYVFRWRTAMNEHYMAHWQHLRHIEGAAQRVQEDTMRFASTLEDMGVSFINAVMTLIAFLPVLVTLSEHVPDLPIVGHL
PYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVEAAYRKELVYGEDDETRATPPTVRELFRAVRRNYFRLYFHYMYF
NIARILYLQVDNVFGLFLLFPSIVAGTITLGLMTQITNVFGQVRGSFQYLISSWTTLVELMSIYKRLRSFERELDGKPLQ
EAIPTLR
>Mature_407_residues
MMFKSFFPKPGPFFISAFIWSLLAVIFWQAGGGDWLLRVTGASQNVAISAARFWSLNYLVFYAYYLFCVGVFALFWFVYC
PHRWQYWSILGTSLIIFVTWFLVEVGVAINAWYAPFYDLIQSALATPHKVSINQFYQEIGVFLGIAIIAVIIGVMNNFFV
SHYVFRWRTAMNEHYMAHWQHLRHIEGAAQRVQEDTMRFASTLEDMGVSFINAVMTLIAFLPVLVTLSEHVPDLPIVGHL
PYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVEAAYRKELVYGEDDETRATPPTVRELFRAVRRNYFRLYFHYMYF
NIARILYLQVDNVFGLFLLFPSIVAGTITLGLMTQITNVFGQVRGSFQYLISSWTTLVELMSIYKRLRSFERELDGKPLQ
EAIPTLR

Specific function: Required for the uptake of the peptide antibiotic microcin B17 (MccB17) [H]

COG id: COG1133

COG function: function code I; ABC-type long-chain fatty acid transport system, fused permease and ATPase components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To R.meliloti BacA [H]

Homologues:

Organism=Escherichia coli, GI1786575, Length=405, Percent_Identity=86.6666666666667, Blast_Score=741, Evalue=0.0,
Organism=Escherichia coli, GI1787772, Length=327, Percent_Identity=23.5474006116208, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011527
- InterPro:   IPR009248 [H]

Pfam domain/function: PF05992 SbmA_BacA [H]

EC number: NA

Molecular weight: Translated: 46868; Mature: 46868

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMFKSFFPKPGPFFISAFIWSLLAVIFWQAGGGDWLLRVTGASQNVAISAARFWSLNYLV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEHHHHHHHHHHHH
FYAYYLFCVGVFALFWFVYCPHRWQYWSILGTSLIIFVTWFLVEVGVAINAWYAPFYDLI
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSALATPHKVSINQFYQEIGVFLGIAIIAVIIGVMNNFFVSHYVFRWRTAMNEHYMAHWQ
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLRHIEGAAQRVQEDTMRFASTLEDMGVSFINAVMTLIAFLPVLVTLSEHVPDLPIVGHL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVEAAYRKELVYGEDDETRATPPTVR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
ELFRAVRRNYFRLYFHYMYFNIARILYLQVDNVFGLFLLFPSIVAGTITLGLMTQITNVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQVRGSFQYLISSWTTLVELMSIYKRLRSFERELDGKPLQEAIPTLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; microcin B17 [Periplasm]; H2O [C]

Specific reaction: ATP + microcin B17 [Periplasm] + H2O = ADP + phosphate + microcin B17 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]