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The map label for this gene is phoE [H]

Identifier: 152968855

GI number: 152968855

Start: 320001

End: 321029

Strand: Direct

Name: phoE [H]

Synonym: KPN_00282

Alternate gene names: 152968855

Gene position: 320001-321029 (Clockwise)

Preceding gene: 152968852

Following gene: 152968862

Centisome position: 6.02

GC content: 54.91

Gene sequence:

>1029_bases
ATGATGATGGGCTTTGTGGCTTCAACAGCGACGCAGGCAGCGGAAGTTTATAATAAGAACGCGAACAAGCTGGATGTGTA
CGGCAAGATCAAAGCCATGCACTATTTCAGCGACTATGACAGCAAGGATGGCGATCAGACCTACGTGCGTTTCGGTATTA
AAGGCGAAACGCAGATTAACGACGACCTGACCGGCTATGGCCGTTGGGAATCTGAATTCTCCGGTAACAAAACCGAGAGC
GACTCCAGCCAGAAAACCCGTCTGGCGTTCGCCGGCGTGAAGCTGAAGAACTACGGCTCCTTCGATTACGGTCGTAACCT
TGGCGCCCTGTACGACGTGGAAGCCTGGACCGATATGTTCCCGGAATTCGGCGGCGATTCCTCTGCCCAGACCGATAACT
TTATGACCAAGCGCGCCAGCGGCCTGGCGACCTACCGCAACACCGACTTCTTCGGTCTGGTGGATGGCCTGGATCTGACC
CTGCAGTACCAGGGTAAAAACGAAGGCCGTGAAGCGAAGAAACAGAACGGCGACGGCGTCGGCACCTCGTTAAGCTATGA
TTTCGGCGGCAGCGACTTCGCCGTCAGCGCAGCCTACACCAGCTCCGACCGTACCAACGATCAGAACCTGCTGGCCCGCG
GCCAGGGTTCGAAAGCGGAAGCCTGGGCGACCGGCCTGAAATATGACGCCAACAATATCTACCTGGCGACCATGTACTCT
GAAACCCGCAAAATGACCCCGATCAGCGGCGGCTTTGCCAACAAAGCGCAGAACTTTGAAGCGGTGGCGCAGTATCAGTT
CGACTTCGGTCTGCGTCCGTCCCTCGGCTATGTGCTGTCGAAAGGGAAGGATATCGAAGGGGTGGGGAGTGAAGATCTGG
TTAACTACATCGACGTGGGCCTGACCTACTACTTCAACAAAAACATGAACGCCTTCGTGGATTACAAAATCAACCAGCTG
AAAAGCGATAACAAACTCGGCATCAACGATGACGACATCGTCGCGCTGGGTATGACCTACCAGTTCTGA

Upstream 100 bases:

>100_bases
TAAAAAACCTCCAGTTTTTGATGGGCGAGCGCCCGCAATTGAATAGCGCGTATAAATAAATCACCAGGACTGAATAATGA
AAAAGAGTACTCTGGCATTA

Downstream 100 bases:

>100_bases
TTGACCGCAGAACCACCCGCCGCAGCCCTGGCTGCGGCTTTTTTTTAGCGGCGATTCTTCGCCAGATGCAGGGTGTCGCT
GTTCATGGTGATCTGCGCGT

Product: outer membrane phosphoporin protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MMMGFVASTATQAAEVYNKNANKLDVYGKIKAMHYFSDYDSKDGDQTYVRFGIKGETQINDDLTGYGRWESEFSGNKTES
DSSQKTRLAFAGVKLKNYGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGLVDGLDLT
LQYQGKNEGREAKKQNGDGVGTSLSYDFGGSDFAVSAAYTSSDRTNDQNLLARGQGSKAEAWATGLKYDANNIYLATMYS
ETRKMTPISGGFANKAQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGVGSEDLVNYIDVGLTYYFNKNMNAFVDYKINQL
KSDNKLGINDDDIVALGMTYQF

Sequences:

>Translated_342_residues
MMMGFVASTATQAAEVYNKNANKLDVYGKIKAMHYFSDYDSKDGDQTYVRFGIKGETQINDDLTGYGRWESEFSGNKTES
DSSQKTRLAFAGVKLKNYGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGLVDGLDLT
LQYQGKNEGREAKKQNGDGVGTSLSYDFGGSDFAVSAAYTSSDRTNDQNLLARGQGSKAEAWATGLKYDANNIYLATMYS
ETRKMTPISGGFANKAQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGVGSEDLVNYIDVGLTYYFNKNMNAFVDYKINQL
KSDNKLGINDDDIVALGMTYQF
>Mature_342_residues
MMMGFVASTATQAAEVYNKNANKLDVYGKIKAMHYFSDYDSKDGDQTYVRFGIKGETQINDDLTGYGRWESEFSGNKTES
DSSQKTRLAFAGVKLKNYGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGLVDGLDLT
LQYQGKNEGREAKKQNGDGVGTSLSYDFGGSDFAVSAAYTSSDRTNDQNLLARGQGSKAEAWATGLKYDANNIYLATMYS
ETRKMTPISGGFANKAQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGVGSEDLVNYIDVGLTYYFNKNMNAFVDYKINQL
KSDNKLGINDDDIVALGMTYQF

Specific function: Uptake of inorganic phosphate, phosphorylated compounds, and some other negatively charged solutes [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Gram-negative porin family [H]

Homologues:

Organism=Escherichia coli, GI1786436, Length=343, Percent_Identity=83.9650145772595, Blast_Score=597, Evalue=1e-172,
Organism=Escherichia coli, GI1787641, Length=356, Percent_Identity=60.1123595505618, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI1787160, Length=352, Percent_Identity=58.8068181818182, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1788544, Length=363, Percent_Identity=57.0247933884297, Blast_Score=392, Evalue=1e-110,

Paralogues:

None

Copy number: 794 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001897
- InterPro:   IPR013793
- InterPro:   IPR001702 [H]

Pfam domain/function: PF00267 Porin_1 [H]

EC number: NA

Molecular weight: Translated: 37851; Mature: 37851

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00576 GRAM_NEG_PORIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMMGFVASTATQAAEVYNKNANKLDVYGKIKAMHYFSDYDSKDGDQTYVRFGIKGETQIN
CCEEEEHHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCCCCCCCCEEEEEECCCCCEEC
DDLTGYGRWESEFSGNKTESDSSQKTRLAFAGVKLKNYGSFDYGRNLGALYDVEAWTDMF
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCEEECHHHHHHH
PEFGGDSSAQTDNFMTKRASGLATYRNTDFFGLVDGLDLTLQYQGKNEGREAKKQNGDGV
HCCCCCCCCCCCHHHHHHHCCCEEECCCCEEEEECCCEEEEEECCCCCCHHHHHCCCCCC
GTSLSYDFGGSDFAVSAAYTSSDRTNDQNLLARGQGSKAEAWATGLKYDANNIYLATMYS
CCEEEECCCCCCEEEEEEECCCCCCCCCHHEECCCCCCCHHHHCCCEECCCCEEEEEEEC
ETRKMTPISGGFANKAQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGVGSEDLVNYIDVG
CCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHCC
LTYYFNKNMNAFVDYKINQLKSDNKLGINDDDIVALGMTYQF
EEEEEECCCCEEEEEEEECCCCCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MMMGFVASTATQAAEVYNKNANKLDVYGKIKAMHYFSDYDSKDGDQTYVRFGIKGETQIN
CCEEEEHHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCCCCCCCCEEEEEECCCCCEEC
DDLTGYGRWESEFSGNKTESDSSQKTRLAFAGVKLKNYGSFDYGRNLGALYDVEAWTDMF
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCEEECHHHHHHH
PEFGGDSSAQTDNFMTKRASGLATYRNTDFFGLVDGLDLTLQYQGKNEGREAKKQNGDGV
HCCCCCCCCCCCHHHHHHHCCCEEECCCCEEEEECCCEEEEEECCCCCCHHHHHCCCCCC
GTSLSYDFGGSDFAVSAAYTSSDRTNDQNLLARGQGSKAEAWATGLKYDANNIYLATMYS
CCEEEECCCCCCEEEEEEECCCCCCCCCHHEECCCCCCCHHHHCCCEECCCCEEEEEEEC
ETRKMTPISGGFANKAQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGVGSEDLVNYIDVG
CCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHCC
LTYYFNKNMNAFVDYKINQLKSDNKLGINDDDIVALGMTYQF
EEEEEECCCCEEEEEEEECCCCCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3032618 [H]