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The map label for this gene is tilS [H]

Identifier: 152968782

GI number: 152968782

Start: 238090

End: 239403

Strand: Direct

Name: tilS [H]

Synonym: KPN_00201

Alternate gene names: 152968782

Gene position: 238090-239403 (Clockwise)

Preceding gene: 152968781

Following gene: 152968785

Centisome position: 4.48

GC content: 65.37

Gene sequence:

>1314_bases
ATGTTTGTGGATATGTTGGCTGATATTGCGCATACGCTTCATCCCCATCGCCAGCTGCTGGTGGCCTTCAGCGGCGGTCT
GGACTCGACGGTTCTCCTGCACCAGCTGGTGCAGCTGCGGGAACAGGATCCCGCGCTGACGCTGCGGGCGGTGCATGTTC
ATCACGGCCTTAGCGCCCATGCAGACCAGTGGGTGGCGCATTGTCGTCAGGTTTGCCAGCAGTGGCAGGTGCCGCTGGTG
GTGCATCACGTGACGTTAGCCAGGGGAGGGCTCGGCGTCGAGGCCCACGCTCGTGCGGCGCGCTATCAGGCCTTTCAGGA
GACGCTCAACGCCGGTGAGGTGTTAGTGACCGCCCAGCACCAGGATGACCAGTGTGAAACCCTGCTGCTGGCGTTAAAAC
GCGGCAGCGGTCCTACCGGCCTGTCGGCGATGGCGCCATCCTCTGCCTTTGCCGGCAGTCGACTACTGCGTCCTCTCCTC
AATGAAACCCGCGAATCACTACGCCAGTGGGCGCTCGCCCATCAGCTGTCGTGGATCGAAGATGAGAGTAATCAGGATGA
TACCTATGACCGCAATTTCCTGCGCCTGCGGGTGATCCCGGTGCTGCGCGAGCGTTGGCCGCACTTTAGCGAGGCCGTCG
CCCGCAGCGCCAGCCTGTGCGCCGAGCAGGAACAACTGCTGGACGAGATGTTGGCGGCGGAGCTGGCGTCGCTGGTGGCC
GAAGATGGCTCGCTGGCTATCGCGCCGCTGGCGACGATGAGTCCGCCGCGCCGAGCGGCGCTGCTGCGTCGCTGGCTCGC
CGGGCAGCAGGCGCCGATGCCCGCCCGCGAGGTGCCTGAGCGCCTCTGGCACGAGGTGGCGCTGGCGCGGGAAGATGCCA
GCCCCTGTCTGCGGCTGGGGGAATTTACCGTCCGCCGTTTTCAACAGCGTTTGTACTGGGTGAAGTATCTTCCCGGTCAG
ACGGACAGCGTTCAGCGCTGGCCCGACTGGCGCCAGCCGCTGCGGCTGGCGGACGGTCTCGGCGAGCTGACGCTGCAGCC
CGGCGGCAGGCTGCGCCCGCCGTCGGCGGATGAGCCGGTCACCGTCCGCTTTCGCGCCAGCGGCCACCTGCATATTGTCG
GGCGCCATGGCGGACGCAAGCTTAAGAAGCTCTGGCAGGAGCTGGGCGTTGCTCCGTGGCGACGAGACACCACGCCGCTG
CTGTTTTACGGCGAAACGCCGATTGCCGCAGCGGACGATCTGTTTGTGACCACAGAAGGTGAAGTGAAGGACGGGGAAGG
GGTCAGGCTGATGTGGAGGAAAACGGGCGATTAA

Upstream 100 bases:

>100_bases
TCTTTAGCGATCCGGACGACCTGCCGCTGGAAATATATCAGCAGTAAACCTTGCCAGCGCGACCCTGGCCCGGTAACGTG
CCGGGCCATCACTTCTCGGT

Downstream 100 bases:

>100_bases
GCCCGTTTTTGATTCAGGATTCGCTCACCACCACGGTGCCGATTTCCGGGTGACTGAAGCTGGCGATTTTATCCAGGCGC
AGCTCGCGGACGTTGCCGGC

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MFVDMLADIAHTLHPHRQLLVAFSGGLDSTVLLHQLVQLREQDPALTLRAVHVHHGLSAHADQWVAHCRQVCQQWQVPLV
VHHVTLARGGLGVEAHARAARYQAFQETLNAGEVLVTAQHQDDQCETLLLALKRGSGPTGLSAMAPSSAFAGSRLLRPLL
NETRESLRQWALAHQLSWIEDESNQDDTYDRNFLRLRVIPVLRERWPHFSEAVARSASLCAEQEQLLDEMLAAELASLVA
EDGSLAIAPLATMSPPRRAALLRRWLAGQQAPMPAREVPERLWHEVALAREDASPCLRLGEFTVRRFQQRLYWVKYLPGQ
TDSVQRWPDWRQPLRLADGLGELTLQPGGRLRPPSADEPVTVRFRASGHLHIVGRHGGRKLKKLWQELGVAPWRRDTTPL
LFYGETPIAAADDLFVTTEGEVKDGEGVRLMWRKTGD

Sequences:

>Translated_437_residues
MFVDMLADIAHTLHPHRQLLVAFSGGLDSTVLLHQLVQLREQDPALTLRAVHVHHGLSAHADQWVAHCRQVCQQWQVPLV
VHHVTLARGGLGVEAHARAARYQAFQETLNAGEVLVTAQHQDDQCETLLLALKRGSGPTGLSAMAPSSAFAGSRLLRPLL
NETRESLRQWALAHQLSWIEDESNQDDTYDRNFLRLRVIPVLRERWPHFSEAVARSASLCAEQEQLLDEMLAAELASLVA
EDGSLAIAPLATMSPPRRAALLRRWLAGQQAPMPAREVPERLWHEVALAREDASPCLRLGEFTVRRFQQRLYWVKYLPGQ
TDSVQRWPDWRQPLRLADGLGELTLQPGGRLRPPSADEPVTVRFRASGHLHIVGRHGGRKLKKLWQELGVAPWRRDTTPL
LFYGETPIAAADDLFVTTEGEVKDGEGVRLMWRKTGD
>Mature_437_residues
MFVDMLADIAHTLHPHRQLLVAFSGGLDSTVLLHQLVQLREQDPALTLRAVHVHHGLSAHADQWVAHCRQVCQQWQVPLV
VHHVTLARGGLGVEAHARAARYQAFQETLNAGEVLVTAQHQDDQCETLLLALKRGSGPTGLSAMAPSSAFAGSRLLRPLL
NETRESLRQWALAHQLSWIEDESNQDDTYDRNFLRLRVIPVLRERWPHFSEAVARSASLCAEQEQLLDEMLAAELASLVA
EDGSLAIAPLATMSPPRRAALLRRWLAGQQAPMPAREVPERLWHEVALAREDASPCLRLGEFTVRRFQQRLYWVKYLPGQ
TDSVQRWPDWRQPLRLADGLGELTLQPGGRLRPPSADEPVTVRFRASGHLHIVGRHGGRKLKKLWQELGVAPWRRDTTPL
LFYGETPIAAADDLFVTTEGEVKDGEGVRLMWRKTGD

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=431, Percent_Identity=66.3573085846868, Blast_Score=559, Evalue=1e-160,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 49076; Mature: 49076

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVDMLADIAHTLHPHRQLLVAFSGGLDSTVLLHQLVQLREQDPALTLRAVHVHHGLSAH
CHHHHHHHHHHHHCHHHHEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEHHCCCCHH
ADQWVAHCRQVCQQWQVPLVVHHVTLARGGLGVEAHARAARYQAFQETLNAGEVLVTAQH
HHHHHHHHHHHHHHCCCCCEEHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECC
QDDQCETLLLALKRGSGPTGLSAMAPSSAFAGSRLLRPLLNETRESLRQWALAHQLSWIE
CCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DESNQDDTYDRNFLRLRVIPVLRERWPHFSEAVARSASLCAEQEQLLDEMLAAELASLVA
CCCCCCCCCCCCEEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDGSLAIAPLATMSPPRRAALLRRWLAGQQAPMPAREVPERLWHEVALAREDASPCLRLG
CCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
EFTVRRFQQRLYWVKYLPGQTDSVQRWPDWRQPLRLADGLGELTLQPGGRLRPPSADEPV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCCCE
TVRFRASGHLHIVGRHGGRKLKKLWQELGVAPWRRDTTPLLFYGETPIAAADDLFVTTEG
EEEEECCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCEEEEECC
EVKDGEGVRLMWRKTGD
CCCCCCCEEEEEEECCC
>Mature Secondary Structure
MFVDMLADIAHTLHPHRQLLVAFSGGLDSTVLLHQLVQLREQDPALTLRAVHVHHGLSAH
CHHHHHHHHHHHHCHHHHEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEHHCCCCHH
ADQWVAHCRQVCQQWQVPLVVHHVTLARGGLGVEAHARAARYQAFQETLNAGEVLVTAQH
HHHHHHHHHHHHHHCCCCCEEHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECC
QDDQCETLLLALKRGSGPTGLSAMAPSSAFAGSRLLRPLLNETRESLRQWALAHQLSWIE
CCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DESNQDDTYDRNFLRLRVIPVLRERWPHFSEAVARSASLCAEQEQLLDEMLAAELASLVA
CCCCCCCCCCCCEEEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDGSLAIAPLATMSPPRRAALLRRWLAGQQAPMPAREVPERLWHEVALAREDASPCLRLG
CCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
EFTVRRFQQRLYWVKYLPGQTDSVQRWPDWRQPLRLADGLGELTLQPGGRLRPPSADEPV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCCCE
TVRFRASGHLHIVGRHGGRKLKKLWQELGVAPWRRDTTPLLFYGETPIAAADDLFVTTEG
EEEEECCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCEEEEECC
EVKDGEGVRLMWRKTGD
CCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]