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The map label for this gene is ispC [H]

Identifier: 152968767

GI number: 152968767

Start: 219232

End: 220434

Strand: Direct

Name: ispC [H]

Synonym: KPN_00186

Alternate gene names: 152968767

Gene position: 219232-220434 (Clockwise)

Preceding gene: 152968766

Following gene: 152968768

Centisome position: 4.12

GC content: 60.35

Gene sequence:

>1203_bases
ATGAAGCAATTAACCGTTCTCGGTTCGACCGGCTCCATTGGCTGTAGCACGCTGGACGTGGTGCGCCATAACCCTGGTCG
CTTTTCCGTCGCCGCGCTGGTCGCGGGGAAAAACGTTGACCGTATGGTTGAACAATGCCTGGAATTTACCCCCCGTTACG
CGGTGATGGACGATGCGCAAAGCGCTGAGCGGTTGCGCACCCGTCTGCATGAGCATGGCAGTCGCACCGAGGTGCTGAGC
GGCCAGCAGGCGGCGGCAGAGGTCGCGGCGCTGGATGAGGTCGATCAGGTCATGGCGGCAATCGTCGGCGCGGCGGGACT
GGTGCCGACGCTGGCGGCGATCCGCGCCGGGAAAACGGTATTGTTAGCGAATAAAGAATCGCTGGTGACCTGCGGTCGCT
TATTCATGGAGGCGGTGCAACAGTCGGGGGCGCGATTGCTACCGGTCGATAGCGAGCATAACGCTATTTTTCAGAGTATG
CCGGAGACAATTCAGCAGCATCTGGGATACGCTGATCTTGCCCAGAACGGCGTCTCGTCTATCCTGCTGACCGGCTCCGG
TGGGCCTTTCCGCGAGACGGCCGTGGCTGAACTGGCGGCGATGACCCCGGATCAGGCCTGCCGTCATCCCAACTGGTCGA
TGGGGCGCAAGATCTCCGTTGATTCGGCCACGATGATGAACAAAGGCCTGGAATATATTGAAGCGCGCTGGCTGTTTAAC
GCCTCGGCCCAGCAGATGGAAGTGCTGATCCATCCGCAGTCGGTGATCCACTCGATGGTTCGCTATCAGGATGGCAGCGT
GCTGGCCCAGCTTGGGGAGCCGGATATGCGCACGCCGATCGCCCATACCATGGGCTGGCCGCAGCGACTGAATTCCGGCG
TTAAACCGCTGGACTTCTGTCAGTTGAGCAATCTGAGCTTCTCCGCGCCGGACTATACGCGATATCCGTGCCTGAAGCTG
GCGATGGACGCGTTTGACGTCGGCCAGGCCGCGACCACGACGCTGAATGCGGCTAATGAAGAGTCGGTTGCCGCGTTTCT
GCATGGCGATATCCGCTTTACCGATATTGCGGCGGTTAACCTGGCGGTGCTGGATAAGATGGACCTGCAGGAGCCGCAAA
GCATTGATGATGTTCTGGTTATCGACGCCGAGGCGCGCGCAATCGCTCACCAGCAGCTGCAGCGGTTGGTTGCGCAGGCG
TAA

Upstream 100 bases:

>100_bases
TGAAGGCTTAATGCCGCCGTCTCATCCCCGATAGTTGCTGCGTAGGGGCTTGCTGGCGGCGTTTTGCTTTTTATTCTGTT
TCCACTCTTCTGGACGTCTC

Downstream 100 bases:

>100_bases
CGCGGTGATATTTGTGGGCGCAAGGCTTCGGTGATATAGTCTGCGCCACCCGATCGTTGCATTTTGTTGTTAAAGATTAC
GGTGGCCGTGACAAAACACG

Product: 1-deoxy-D-xylulose 5-phosphate reductoisomerase

Products: NA

Alternate protein names: DXP reductoisomerase; 1-deoxyxylulose-5-phosphate reductoisomerase; 2-C-methyl-D-erythritol 4-phosphate synthase [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKQLTVLGSTGSIGCSTLDVVRHNPGRFSVAALVAGKNVDRMVEQCLEFTPRYAVMDDAQSAERLRTRLHEHGSRTEVLS
GQQAAAEVAALDEVDQVMAAIVGAAGLVPTLAAIRAGKTVLLANKESLVTCGRLFMEAVQQSGARLLPVDSEHNAIFQSM
PETIQQHLGYADLAQNGVSSILLTGSGGPFRETAVAELAAMTPDQACRHPNWSMGRKISVDSATMMNKGLEYIEARWLFN
ASAQQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDMRTPIAHTMGWPQRLNSGVKPLDFCQLSNLSFSAPDYTRYPCLKL
AMDAFDVGQAATTTLNAANEESVAAFLHGDIRFTDIAAVNLAVLDKMDLQEPQSIDDVLVIDAEARAIAHQQLQRLVAQA

Sequences:

>Translated_400_residues
MKQLTVLGSTGSIGCSTLDVVRHNPGRFSVAALVAGKNVDRMVEQCLEFTPRYAVMDDAQSAERLRTRLHEHGSRTEVLS
GQQAAAEVAALDEVDQVMAAIVGAAGLVPTLAAIRAGKTVLLANKESLVTCGRLFMEAVQQSGARLLPVDSEHNAIFQSM
PETIQQHLGYADLAQNGVSSILLTGSGGPFRETAVAELAAMTPDQACRHPNWSMGRKISVDSATMMNKGLEYIEARWLFN
ASAQQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDMRTPIAHTMGWPQRLNSGVKPLDFCQLSNLSFSAPDYTRYPCLKL
AMDAFDVGQAATTTLNAANEESVAAFLHGDIRFTDIAAVNLAVLDKMDLQEPQSIDDVLVIDAEARAIAHQQLQRLVAQA
>Mature_400_residues
MKQLTVLGSTGSIGCSTLDVVRHNPGRFSVAALVAGKNVDRMVEQCLEFTPRYAVMDDAQSAERLRTRLHEHGSRTEVLS
GQQAAAEVAALDEVDQVMAAIVGAAGLVPTLAAIRAGKTVLLANKESLVTCGRLFMEAVQQSGARLLPVDSEHNAIFQSM
PETIQQHLGYADLAQNGVSSILLTGSGGPFRETAVAELAAMTPDQACRHPNWSMGRKISVDSATMMNKGLEYIEARWLFN
ASAQQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDMRTPIAHTMGWPQRLNSGVKPLDFCQLSNLSFSAPDYTRYPCLKL
AMDAFDVGQAATTTLNAANEESVAAFLHGDIRFTDIAAVNLAVLDKMDLQEPQSIDDVLVIDAEARAIAHQQLQRLVAQA

Specific function: Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) [H]

COG id: COG0743

COG function: function code I; 1-deoxy-D-xylulose 5-phosphate reductoisomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DXR family [H]

Homologues:

Organism=Escherichia coli, GI1786369, Length=398, Percent_Identity=80.9045226130653, Blast_Score=661, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003821
- InterPro:   IPR013644
- InterPro:   IPR013512
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08436 DXP_redisom_C; PF02670 DXP_reductoisom [H]

EC number: =1.1.1.267 [H]

Molecular weight: Translated: 43236; Mature: 43236

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLTVLGSTGSIGCSTLDVVRHNPGRFSVAALVAGKNVDRMVEQCLEFTPRYAVMDDAQ
CCCEEEECCCCCCCCHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEECCCHH
SAERLRTRLHEHGSRTEVLSGQQAAAEVAALDEVDQVMAAIVGAAGLVPTLAAIRAGKTV
HHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LLANKESLVTCGRLFMEAVQQSGARLLPVDSEHNAIFQSMPETIQQHLGYADLAQNGVSS
EEECCHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCE
ILLTGSGGPFRETAVAELAAMTPDQACRHPNWSMGRKISVDSATMMNKGLEYIEARWLFN
EEEECCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCC
ASAQQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDMRTPIAHTMGWPQRLNSGVKPLDFC
CCHHHHEEEECCHHHHHHHHHCCCCCHHEECCCCCCCCCHHHHCCCHHHHCCCCCCHHHH
QLSNLSFSAPDYTRYPCLKLAMDAFDVGQAATTTLNAANEESVAAFLHGDIRFTDIAAVN
EECCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHEECCCCCHHEEHEEECCEEEEHHHHHH
LAVLDKMDLQEPQSIDDVLVIDAEARAIAHQQLQRLVAQA
HHHHCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKQLTVLGSTGSIGCSTLDVVRHNPGRFSVAALVAGKNVDRMVEQCLEFTPRYAVMDDAQ
CCCEEEECCCCCCCCHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEECCCHH
SAERLRTRLHEHGSRTEVLSGQQAAAEVAALDEVDQVMAAIVGAAGLVPTLAAIRAGKTV
HHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LLANKESLVTCGRLFMEAVQQSGARLLPVDSEHNAIFQSMPETIQQHLGYADLAQNGVSS
EEECCHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCE
ILLTGSGGPFRETAVAELAAMTPDQACRHPNWSMGRKISVDSATMMNKGLEYIEARWLFN
EEEECCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCC
ASAQQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDMRTPIAHTMGWPQRLNSGVKPLDFC
CCHHHHEEEECCHHHHHHHHHCCCCCHHEECCCCCCCCCHHHHCCCHHHHCCCCCCHHHH
QLSNLSFSAPDYTRYPCLKLAMDAFDVGQAATTTLNAANEESVAAFLHGDIRFTDIAAVN
EECCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHEECCCCCHHEEHEEECCEEEEHHHHHH
LAVLDKMDLQEPQSIDDVLVIDAEARAIAHQQLQRLVAQA
HHHHCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]