| Definition | Klebsiella pneumoniae subsp. pneumoniae MGH 78578 chromosome, complete genome. |
|---|---|
| Accession | NC_009648 |
| Length | 5,315,120 |
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The map label for this gene is mrcB [H]
Identifier: 152968745
GI number: 152968745
Start: 191543
End: 194107
Strand: Direct
Name: mrcB [H]
Synonym: KPN_00164
Alternate gene names: 152968745
Gene position: 191543-194107 (Clockwise)
Preceding gene: 152968744
Following gene: 152968746
Centisome position: 3.6
GC content: 61.01
Gene sequence:
>2565_bases ATGGCGGGAGATGACCGCGAGCCTATCGGACGTAAGGGAAGACCCTCACGTCCGACTAAACAGAAAGTCACCCGTCGCCG GGTCCGGGAAGAGGACTACGACGACGAGTATGATGACGATGATTATGAGGATGAGAAACCGGTGCCGAGAAAAGCCAAAG GCAAAGGTGGCAAGCCTCGTCGGAAGCGCAGCTGGCTGTGGCTGCTGGTCAAGCTGGGGATTGTCTTCGCGGTGCTGATT GCCGCCTATGGCGTCTACCTCGACCAGAAAATCCGCAGCCGCATTGACGGTAAAGTCTGGGAGCTGCCGGCCGCGGTCTA TGGCCGGATGGTCAACCTTGAGCCGGACATGCAGATCAGCAAAAACGAGATGGTGCGTCTGCTCAACGCCACGCAGTATC GCCAGGTCAGCGCGATGACGCGCCCCGGCGAATATACCGTGCAGGCTAACAGCATCGAGATGATCCGTCGTCCGTTCGAT TTCCCGGACAGTAAGGAAGGGCAGGTGCGCGCGCGTCTTACCTTCGACGGCGACCATCTTGAGACCATCGAGAATATGGA TAATAACCGCCAGTTCGGTTTCTTCCGCCTCGATCCGCGTCTTATCACCATGCTGCAGTCGCCGAACGGCGAGCAGCGCC TGTTCGTGAAGCGCAGCGGCTTCCCCGATCTGCTGGTGGATACCCTGCTGGCGACCGAAGACCGCCACTTCTATGAGCAC GATGGGATCAGTCTCTACTCCATCGGCCGCGCGGTGCTGGCGAACCTGACGGCAGGCCGAACGGTGCAGGGGGCGAGTAC CCTGACCCAACAGCTGGTGAAAAACCTGTTCCTCTCCAGCGAACGCTCCTACTGGCGTAAAGCCAACGAAGCGTATATGG CGCTGATCGTCGACGCCCGCTACAGCAAGGATCGCATCCTTGAGCTGTATATGAACGAGGTGTACCTCGGCCAGAGCGGC GATAACGAAATTCGCGGCTTCCCGCTGGCCAGCCTGTATTACTTCGGCCGTCCGGTGGAAGAGCTGAGCCTCGACCAGCA GGCGCTGCTGGTGGGGATGGTGAAAGGGGCGTCGGTCTATAACCCGTGGCGTAACCCGAAACTGGCCCTTGAACGACGCA ATCTGGTGCTGCGTCTGCTGCAGCAGCAGCAGGTTATCGACCAGGAGCTGTACGACATGCTCAGCGCCCGTCCGCTGGGC GTGCAGCCGCGCGGTGGGGTGATTTCACCGCAGCCGGCGTTTATGCAGATGGTTCGTCAGGAACTGCAGGCGAAGCTGGG CGATAAAGTGAAAGATCTCTCCGGCGTGAAGATCTTCACCACTTTCGACTCGGTGGCGCAGGATGCGGCGGAAAAAGCGG CCTCCGAAGGGATCCCGGTTCTGAAGAAACAGCGTAAGCTGGCAGACCTGGAAACCGCCATGGTGGTGGTTGACCGCTTC ACCGGCGAAGTGCGGGCGATGGTCGGCGGCGCGGAGCCGCAGTTCGCCGGTTATAACCGCGCGATGCAGGCCCGCCGATC GATTGGTTCTCTGGCCAAGCCGGCGACCTATCTGACCGCGCTGAGCCAGCCGAATCAGTATCGCCTGAATACCTGGATCG CCGATGCGCCGGTGACGATTCGCCTGTCCAACGGTCAGACGTGGTCCCCGCAGAACGACGATCGTCGTTTCAGCGGTCAG GTGATGCTGGTCGATGCCCTGACTCGCTCAATGAACGTCCCGACGGTTAACCTCGGGATGGCGCTGGGCTTGCCGGCGGT AGTCGATACCTGGACTAAACTGGGGGCGCCGAAGAATCAGCTCAACGCGGTGCCGTCGATGCTGCTTGGCGCGCTGAACC TGACACCGATTGAAGTGGCCCAGGCCTTCCAGACCATCGCCAGCGGTGGGAACCGTGCGCCGCTTTCCGCGCTGCGGTCA GTGATTGCCGAAGATGGCACTGTGCTCTACCAGAGCTATCCGCAGGCGGAGCGCGCGGTGCCTGCCCAGGCGGCCTACAT GACGCTGTGGACTATGCAGCAGGTGGTGCAGCGCGGTACGGGGCGTCAGCTGGGGGCGAAGTATCCGGGCCTGCATCTGG CGGGGAAAACCGGGACCACCAACAATAACGTCGATACCTGGTTCGCCGGCATCGACGGTAGTCAGGTGACCATCACCTGG GTGGGGCGCGACAATAACCAGCCGACCAAACTGTACGGTGCCAGCGGCGCGATGTCGATTTACCAGCGCTATCTGGCGAA CCAGACGCCAACGCCGCTGGTGCTGACGGTGCCGGAAGACGTGGTTGATATGGGCGTGGACAGCAACGGCAACTTCGTCT GCAGCGGTGGTATGCGTTCGCTGCCGGTCTGGACCACGCAGCCGGATGCGCTGTGCCGTCAGGGCGAAATGATGCAACAG CAGCAGTTGCAGCAACAGGAAGCGAAAAATCCGTTTAATCAGTCCGGGCAGCAGCAGCAGCAGCAACAGCAGCAACAGCA GCAACAGCAGCAACAGCCGCCGAAGCAGCAGGAAAAGAGCGACGGCGTCGCGGGCTGGATCAAGGATATGTTCGGCAATA ACTGA
Upstream 100 bases:
>100_bases ATAAGCAGCCTGGACGTTAATTTGAGAGATTTCTTCTTCCTCTGAACAGGGGAAGTATTGAGAATCAGGCCTTTGCGCCT GAACGTTGCGGAGAAAAAGC
Downstream 100 bases:
>100_bases TTTTGACCGCACGACCAGGCCCCTTAATACGAAAGTATTAAGGGGTTTTTTTATGCCTGTAAAAAAGTTAACGGCTCTTT AACTCCATAATAACTATCTG
Product: penicillin-binding protein 1b
Products: NA
Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 854; Mature: 853
Protein sequence:
>854_residues MAGDDREPIGRKGRPSRPTKQKVTRRRVREEDYDDEYDDDDYEDEKPVPRKAKGKGGKPRRKRSWLWLLVKLGIVFAVLI AAYGVYLDQKIRSRIDGKVWELPAAVYGRMVNLEPDMQISKNEMVRLLNATQYRQVSAMTRPGEYTVQANSIEMIRRPFD FPDSKEGQVRARLTFDGDHLETIENMDNNRQFGFFRLDPRLITMLQSPNGEQRLFVKRSGFPDLLVDTLLATEDRHFYEH DGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFLSSERSYWRKANEAYMALIVDARYSKDRILELYMNEVYLGQSG DNEIRGFPLASLYYFGRPVEELSLDQQALLVGMVKGASVYNPWRNPKLALERRNLVLRLLQQQQVIDQELYDMLSARPLG VQPRGGVISPQPAFMQMVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEKAASEGIPVLKKQRKLADLETAMVVVDRF TGEVRAMVGGAEPQFAGYNRAMQARRSIGSLAKPATYLTALSQPNQYRLNTWIADAPVTIRLSNGQTWSPQNDDRRFSGQ VMLVDALTRSMNVPTVNLGMALGLPAVVDTWTKLGAPKNQLNAVPSMLLGALNLTPIEVAQAFQTIASGGNRAPLSALRS VIAEDGTVLYQSYPQAERAVPAQAAYMTLWTMQQVVQRGTGRQLGAKYPGLHLAGKTGTTNNNVDTWFAGIDGSQVTITW VGRDNNQPTKLYGASGAMSIYQRYLANQTPTPLVLTVPEDVVDMGVDSNGNFVCSGGMRSLPVWTTQPDALCRQGEMMQQ QQLQQQEAKNPFNQSGQQQQQQQQQQQQQQQQPPKQQEKSDGVAGWIKDMFGNN
Sequences:
>Translated_854_residues MAGDDREPIGRKGRPSRPTKQKVTRRRVREEDYDDEYDDDDYEDEKPVPRKAKGKGGKPRRKRSWLWLLVKLGIVFAVLI AAYGVYLDQKIRSRIDGKVWELPAAVYGRMVNLEPDMQISKNEMVRLLNATQYRQVSAMTRPGEYTVQANSIEMIRRPFD FPDSKEGQVRARLTFDGDHLETIENMDNNRQFGFFRLDPRLITMLQSPNGEQRLFVKRSGFPDLLVDTLLATEDRHFYEH DGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFLSSERSYWRKANEAYMALIVDARYSKDRILELYMNEVYLGQSG DNEIRGFPLASLYYFGRPVEELSLDQQALLVGMVKGASVYNPWRNPKLALERRNLVLRLLQQQQVIDQELYDMLSARPLG VQPRGGVISPQPAFMQMVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEKAASEGIPVLKKQRKLADLETAMVVVDRF TGEVRAMVGGAEPQFAGYNRAMQARRSIGSLAKPATYLTALSQPNQYRLNTWIADAPVTIRLSNGQTWSPQNDDRRFSGQ VMLVDALTRSMNVPTVNLGMALGLPAVVDTWTKLGAPKNQLNAVPSMLLGALNLTPIEVAQAFQTIASGGNRAPLSALRS VIAEDGTVLYQSYPQAERAVPAQAAYMTLWTMQQVVQRGTGRQLGAKYPGLHLAGKTGTTNNNVDTWFAGIDGSQVTITW VGRDNNQPTKLYGASGAMSIYQRYLANQTPTPLVLTVPEDVVDMGVDSNGNFVCSGGMRSLPVWTTQPDALCRQGEMMQQ QQLQQQEAKNPFNQSGQQQQQQQQQQQQQQQQPPKQQEKSDGVAGWIKDMFGNN >Mature_853_residues AGDDREPIGRKGRPSRPTKQKVTRRRVREEDYDDEYDDDDYEDEKPVPRKAKGKGGKPRRKRSWLWLLVKLGIVFAVLIA AYGVYLDQKIRSRIDGKVWELPAAVYGRMVNLEPDMQISKNEMVRLLNATQYRQVSAMTRPGEYTVQANSIEMIRRPFDF PDSKEGQVRARLTFDGDHLETIENMDNNRQFGFFRLDPRLITMLQSPNGEQRLFVKRSGFPDLLVDTLLATEDRHFYEHD GISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFLSSERSYWRKANEAYMALIVDARYSKDRILELYMNEVYLGQSGD NEIRGFPLASLYYFGRPVEELSLDQQALLVGMVKGASVYNPWRNPKLALERRNLVLRLLQQQQVIDQELYDMLSARPLGV QPRGGVISPQPAFMQMVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEKAASEGIPVLKKQRKLADLETAMVVVDRFT GEVRAMVGGAEPQFAGYNRAMQARRSIGSLAKPATYLTALSQPNQYRLNTWIADAPVTIRLSNGQTWSPQNDDRRFSGQV MLVDALTRSMNVPTVNLGMALGLPAVVDTWTKLGAPKNQLNAVPSMLLGALNLTPIEVAQAFQTIASGGNRAPLSALRSV IAEDGTVLYQSYPQAERAVPAQAAYMTLWTMQQVVQRGTGRQLGAKYPGLHLAGKTGTTNNNVDTWFAGIDGSQVTITWV GRDNNQPTKLYGASGAMSIYQRYLANQTPTPLVLTVPEDVVDMGVDSNGNFVCSGGMRSLPVWTTQPDALCRQGEMMQQQ QLQQQEAKNPFNQSGQQQQQQQQQQQQQQQQPPKQQEKSDGVAGWIKDMFGNN
Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal
COG id: COG0744
COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)
Gene ontology:
Cell location: Cell inner membrane; Single-pass type II membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI1786343, Length=856, Percent_Identity=86.6822429906542, Blast_Score=1413, Evalue=0.0, Organism=Escherichia coli, GI87082258, Length=249, Percent_Identity=34.9397590361446, Blast_Score=153, Evalue=4e-38, Organism=Escherichia coli, GI1788867, Length=580, Percent_Identity=27.5862068965517, Blast_Score=135, Evalue=1e-32, Organism=Escherichia coli, GI1789601, Length=127, Percent_Identity=37.007874015748, Blast_Score=79, Evalue=2e-15,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011813 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: =2.4.1.129 [H]
Molecular weight: Translated: 95662; Mature: 95531
Theoretical pI: Translated: 9.60; Mature: 9.60
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 3.4 %Met (Translated Protein) 3.6 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 3.3 %Met (Mature Protein) 3.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAGDDREPIGRKGRPSRPTKQKVTRRRVREEDYDDEYDDDDYEDEKPVPRKAKGKGGKPR CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC RKRSWLWLLVKLGIVFAVLIAAYGVYLDQKIRSRIDGKVWELPAAVYGRMVNLEPDMQIS HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHCEEECCCCCCCCC KNEMVRLLNATQYRQVSAMTRPGEYTVQANSIEMIRRPFDFPDSKEGQVRARLTFDGDHL HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHCCCCCCCCCCCCEEEEEEECCHHH ETIENMDNNRQFGFFRLDPRLITMLQSPNGEQRLFVKRSGFPDLLVDTLLATEDRHFYEH HHHHCCCCCCCEEEEEECHHHHHEECCCCCCCEEEEECCCCHHHHHHHHHHCCCCCHHHC DGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFLSSERSYWRKANEAYMALIVDAR CCEEHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEEEEECC YSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYFGRPVEELSLDQQALLVGMVKGASVY CCHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHCCCHHHHCCCHHHHHHHHHCCCCCC NPWRNPKLALERRNLVLRLLQQQQVIDQELYDMLSARPLGVQPRGGVISPQPAFMQMVRQ CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH ELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEKAASEGIPVLKKQRKLADLETAMVVVDRF HHHHHHCCHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH TGEVRAMVGGAEPQFAGYNRAMQARRSIGSLAKPATYLTALSQPNQYRLNTWIADAPVTI CCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEEECCCEEE RLSNGQTWSPQNDDRRFSGQVMLVDALTRSMNVPTVNLGMALGLPAVVDTWTKLGAPKNQ EECCCCCCCCCCCCCCCCCCEEEEEHHHHCCCCCEEECHHHHCCHHHHHHHHHCCCCHHH LNAVPSMLLGALNLTPIEVAQAFQTIASGGNRAPLSALRSVIAEDGTVLYQSYPQAERAV HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHCC PAQAAYMTLWTMQQVVQRGTGRQLGAKYPGLHLAGKTGTTNNNVDTWFAGIDGSQVTITW CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEECCCCCEEEEEE VGRDNNQPTKLYGASGAMSIYQRYLANQTPTPLVLTVPEDVVDMGVDSNGNFVCSGGMRS EECCCCCCCEEECCCHHHHHHHHHHCCCCCCCEEEECCHHHHHCCCCCCCCEEECCCCCC LPVWTTQPDALCRQGEMMQQQQLQQQEAKNPFNQSGQQQQQQQQQQQQQQQQPPKQQEKS CCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHC DGVAGWIKDMFGNN CCHHHHHHHHCCCC >Mature Secondary Structure AGDDREPIGRKGRPSRPTKQKVTRRRVREEDYDDEYDDDDYEDEKPVPRKAKGKGGKPR CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC RKRSWLWLLVKLGIVFAVLIAAYGVYLDQKIRSRIDGKVWELPAAVYGRMVNLEPDMQIS HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHCEEECCCCCCCCC KNEMVRLLNATQYRQVSAMTRPGEYTVQANSIEMIRRPFDFPDSKEGQVRARLTFDGDHL HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHCCCCCCCCCCCCEEEEEEECCHHH ETIENMDNNRQFGFFRLDPRLITMLQSPNGEQRLFVKRSGFPDLLVDTLLATEDRHFYEH HHHHCCCCCCCEEEEEECHHHHHEECCCCCCCEEEEECCCCHHHHHHHHHHCCCCCHHHC DGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFLSSERSYWRKANEAYMALIVDAR CCEEHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEEEEECC YSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYFGRPVEELSLDQQALLVGMVKGASVY CCHHHHHHHHHHHHEECCCCCCCCCCCCHHHHHHHCCCHHHHCCCHHHHHHHHHCCCCCC NPWRNPKLALERRNLVLRLLQQQQVIDQELYDMLSARPLGVQPRGGVISPQPAFMQMVRQ CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH ELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEKAASEGIPVLKKQRKLADLETAMVVVDRF HHHHHHCCHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH TGEVRAMVGGAEPQFAGYNRAMQARRSIGSLAKPATYLTALSQPNQYRLNTWIADAPVTI CCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEEECCCEEE RLSNGQTWSPQNDDRRFSGQVMLVDALTRSMNVPTVNLGMALGLPAVVDTWTKLGAPKNQ EECCCCCCCCCCCCCCCCCCEEEEEHHHHCCCCCEEECHHHHCCHHHHHHHHHCCCCHHH LNAVPSMLLGALNLTPIEVAQAFQTIASGGNRAPLSALRSVIAEDGTVLYQSYPQAERAV HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHCC PAQAAYMTLWTMQQVVQRGTGRQLGAKYPGLHLAGKTGTTNNNVDTWFAGIDGSQVTITW CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEECCCCCEEEEEE VGRDNNQPTKLYGASGAMSIYQRYLANQTPTPLVLTVPEDVVDMGVDSNGNFVCSGGMRS EECCCCCCCEEECCCHHHHHHHHHHCCCCCCCEEEECCHHHHHCCCCCCCCEEECCCCCC LPVWTTQPDALCRQGEMMQQQQLQQQEAKNPFNQSGQQQQQQQQQQQQQQQQPPKQQEKS CCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHC DGVAGWIKDMFGNN CCHHHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 3882429; 8202364; 9278503; 3920658; 8645198; 9244263; 3330753; 1885547; 10037771; 10564478; 9841666 [H]