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The map label for this gene is ddl [H]

Identifier: 152968677

GI number: 152968677

Start: 113999

End: 114919

Strand: Direct

Name: ddl [H]

Synonym: KPN_00096

Alternate gene names: 152968677

Gene position: 113999-114919 (Clockwise)

Preceding gene: 152968676

Following gene: 152968678

Centisome position: 2.14

GC content: 56.35

Gene sequence:

>921_bases
ATGGCTGATAAAATCGCGGTGCTTTTCGGTGGAACCTCCGCTGAACGTGAAGTGTCGCTGAATTCAGGCGCGGCGGTGCT
GGCCGGGCTGCGTGAAGGCGGGGTGGACGCCCATCCGGTCGACCCGCGCGATGTTGATATTACCCAGCTAAAAAAGCAGG
GCTTTAAGAAAGCGTTTATTGCCCTCCACGGCCGCGGCGGGGAAGATGGCACTCTGCAGGGGCTGCTGGAGTTAATCCAG
CTGCCTTATACCGGCAGCGGCGTGATGGCTTCGGCGATTTCAATGGATAAACTGCGCAGCAAGTGGCTGTGGCAGGGCGC
CGGATTACCGGTCGCGCCGTGGGTGGCGCTGACCCGCGCCCAGTTTACCGCCGGGCTGTCTACTGACGTCGAACAGCAAA
TTGCGGCGCTGGGTCTGCCGGTCATCATCAAGCCGAGCCGTGAAGGCTCCAGCGTCGGGATGTCGAAAGTCAGCGAAAGT
TGTGATTTAGCGAGTGCATTGGCGTTGGCTTTTCAGCATGATGATGAAGTTTTGGTCGAAAAATGGCTCAGTGGACCGGA
ATTTACCGTCGCCATAGTCGGTGAAGAAATTTTACCGTCAATACGTATCCAGGCCGCGGGAACCTTCTATGATTATGAGG
CGAAGTATCTCTCTGATGAGACGCAATATTTCTGCCCAGGATTTGAAGATCCCGCGCGTGAGTCAGCTATTCAGAACCTG
GTGCTTAAAGCCTGGAATGTTCTGGGTTGCAAAGGCTGGGGCCGTATTGACGTGATGCTGGACAGCGATGGCCAGTTTTA
TCTGCTGGAAGCGAATACTTCCCCGGGCATGACCAGCCATAGCCTTGTGCCAATGGCGGCGCGTCAGGCAGGAATGAGCT
TCTCCCAGCTTGTTGTACGAATTCTGGACCTGGCGGGGTGA

Upstream 100 bases:

>100_bases
AACGATTTAGTACTGGTGCAGGGCGCCGGTAACATCGGTAAAATCGCCCGCCACCTGGCTGAAATCAAACTGATCCCGCA
AAAAACGGAGGAGGAGCGCC

Downstream 100 bases:

>100_bases
TATGTCGCAGGCTGCGCTGAATACGCGGAACCATGAAGAAGAGGCTTCTTCTTCGCGTCGGAGTAATGGAACGCGGCTGG
CAGGGATCGTTTTCCTGCTG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase B; D-alanylalanine synthetase B [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MADKIAVLFGGTSAEREVSLNSGAAVLAGLREGGVDAHPVDPRDVDITQLKKQGFKKAFIALHGRGGEDGTLQGLLELIQ
LPYTGSGVMASAISMDKLRSKWLWQGAGLPVAPWVALTRAQFTAGLSTDVEQQIAALGLPVIIKPSREGSSVGMSKVSES
CDLASALALAFQHDDEVLVEKWLSGPEFTVAIVGEEILPSIRIQAAGTFYDYEAKYLSDETQYFCPGFEDPARESAIQNL
VLKAWNVLGCKGWGRIDVMLDSDGQFYLLEANTSPGMTSHSLVPMAARQAGMSFSQLVVRILDLAG

Sequences:

>Translated_306_residues
MADKIAVLFGGTSAEREVSLNSGAAVLAGLREGGVDAHPVDPRDVDITQLKKQGFKKAFIALHGRGGEDGTLQGLLELIQ
LPYTGSGVMASAISMDKLRSKWLWQGAGLPVAPWVALTRAQFTAGLSTDVEQQIAALGLPVIIKPSREGSSVGMSKVSES
CDLASALALAFQHDDEVLVEKWLSGPEFTVAIVGEEILPSIRIQAAGTFYDYEAKYLSDETQYFCPGFEDPARESAIQNL
VLKAWNVLGCKGWGRIDVMLDSDGQFYLLEANTSPGMTSHSLVPMAARQAGMSFSQLVVRILDLAG
>Mature_305_residues
ADKIAVLFGGTSAEREVSLNSGAAVLAGLREGGVDAHPVDPRDVDITQLKKQGFKKAFIALHGRGGEDGTLQGLLELIQL
PYTGSGVMASAISMDKLRSKWLWQGAGLPVAPWVALTRAQFTAGLSTDVEQQIAALGLPVIIKPSREGSSVGMSKVSESC
DLASALALAFQHDDEVLVEKWLSGPEFTVAIVGEEILPSIRIQAAGTFYDYEAKYLSDETQYFCPGFEDPARESAIQNLV
LKAWNVLGCKGWGRIDVMLDSDGQFYLLEANTSPGMTSHSLVPMAARQAGMSFSQLVVRILDLAG

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=305, Percent_Identity=82.2950819672131, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI1786579, Length=349, Percent_Identity=31.8051575931232, Blast_Score=170, Evalue=9e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 32732; Mature: 32601

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKIAVLFGGTSAEREVSLNSGAAVLAGLREGGVDAHPVDPRDVDITQLKKQGFKKAFI
CCCEEEEEECCCCCCCEEECCCCHHEEEHHHHCCCCCCCCCCCCCCHHHHHHHCCCEEEE
ALHGRGGEDGTLQGLLELIQLPYTGSGVMASAISMDKLRSKWLWQGAGLPVAPWVALTRA
EEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
QFTAGLSTDVEQQIAALGLPVIIKPSREGSSVGMSKVSESCDLASALALAFQHDDEVLVE
HHHCCCCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
KWLSGPEFTVAIVGEEILPSIRIQAAGTFYDYEAKYLSDETQYFCPGFEDPARESAIQNL
HHCCCCCEEEEEECHHHCCCEEEEECCEEEECCHHHCCCCCCEECCCCCCHHHHHHHHHH
VLKAWNVLGCKGWGRIDVMLDSDGQFYLLEANTSPGMTSHSLVPMAARQAGMSFSQLVVR
HHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHH
ILDLAG
HHHHCC
>Mature Secondary Structure 
ADKIAVLFGGTSAEREVSLNSGAAVLAGLREGGVDAHPVDPRDVDITQLKKQGFKKAFI
CCEEEEEECCCCCCCEEECCCCHHEEEHHHHCCCCCCCCCCCCCCHHHHHHHCCCEEEE
ALHGRGGEDGTLQGLLELIQLPYTGSGVMASAISMDKLRSKWLWQGAGLPVAPWVALTRA
EEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
QFTAGLSTDVEQQIAALGLPVIIKPSREGSSVGMSKVSESCDLASALALAFQHDDEVLVE
HHHCCCCCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
KWLSGPEFTVAIVGEEILPSIRIQAAGTFYDYEAKYLSDETQYFCPGFEDPARESAIQNL
HHCCCCCEEEEEECHHHCCCEEEEECCEEEECCHHHCCCCCCEECCCCCCHHHHHHHHHH
VLKAWNVLGCKGWGRIDVMLDSDGQFYLLEANTSPGMTSHSLVPMAARQAGMSFSQLVVR
HHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHH
ILDLAG
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]