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The map label for this gene is citE2

Identifier: 152968617

GI number: 152968617

Start: 37512

End: 38381

Strand: Direct

Name: citE2

Synonym: KPN_00036

Alternate gene names: 152968617

Gene position: 37512-38381 (Clockwise)

Preceding gene: 152968616

Following gene: 152968618

Centisome position: 0.71

GC content: 58.51

Gene sequence:

>870_bases
ATGAAACCACGTCGCAGTATGTTGTTCATCCCTGGCGCCAATGCCGCCATGTTAAGCACGTCATTCGTCTACGGCGCCGA
TGCGGTGATGTTCGACCTGGAAGATGCCGTTTCGCTGCGCGAGAAAGATACCGCTCGTCTGCTGGTGTATCAGGCGCTGC
AGCATCCACTGTATCAGGATATCGAAACCGTGGTGCGCATTAACCCGCTGAATACGCCGTTCGGTCTTGCCGACCTTGAA
GCGGTAGTCCGCGCGGGCGTGGATATGGTGCGTCTGCCGAAAACCGACAGCAAAGAAGATATCCATGAGCTGGAAGCGCA
TGTTGAGCGGATTGAACGCGAGTGCGGCCGGGAAGTGGGCAGCACCAAGTTAATGGCGGCGATCGAGTCGGCGCTGGGCG
TGGTGAACGCGGTGGAAATCGCCCGTGCCAGCCCGCGTCTGGCGGCGATCGCGCTGGCGGCCTTCGATTACGTGATGGAT
ATGGGCACCTCCCGCGGCGACGGTACTGAACTGTTCTACGCCCGCTGCGCTGTACTGCATGCCGCCCGCGTTGCCGGCAT
CGCCGCCTATGACGTGGTGTGGTCGGATATCAATAATGAAGAGGGCTTCCTGGCGGAAGCGAATCTGGCCAAAAACCTCG
GCTTTAACGGCAAATCGTTGGTTAACCCACGACAAATTGAACTCCTGCATCAGGTCTATGCCCCGACGCGCAAAGAGGTC
GATCACGCGCTGGAAGTGATTGCCGCGGCGGAAGAAGCCGAAACGCGAGGTCTGGGTGTGGTATCGCTGAACGGCAAGAT
GATCGATGGACCGATTATCGACCATGCTCGCAAAGTGGTGGCGCTCTCGGCTTCCGGTATTCGTGATTAA

Upstream 100 bases:

>100_bases
TGGTCGATGATAAAGGCGCGCTGGAATGTGTTTTGCGAGCTCGCGTACAGGCCGCGGCGCTGCGCGCGGCGCAACAGACC
CAATTACAATGGAGCCAGCT

Downstream 100 bases:

>100_bases
GGGGAATAAGATGAAAGAGACAGTAGCAATGCTTAATCAGCAGTACGTGATGCCGAATGGACTGACACCTTATGCCGGCG
TAACGGCGAAAAGTCCCTGG

Product: putative citrate lyase beta chain (acyl lyase subunit)

Products: NA

Alternate protein names: Citrase beta chain; Citrate (pro-3S)-lyase subunit beta; Citryl-CoA lyase subunit

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MKPRRSMLFIPGANAAMLSTSFVYGADAVMFDLEDAVSLREKDTARLLVYQALQHPLYQDIETVVRINPLNTPFGLADLE
AVVRAGVDMVRLPKTDSKEDIHELEAHVERIERECGREVGSTKLMAAIESALGVVNAVEIARASPRLAAIALAAFDYVMD
MGTSRGDGTELFYARCAVLHAARVAGIAAYDVVWSDINNEEGFLAEANLAKNLGFNGKSLVNPRQIELLHQVYAPTRKEV
DHALEVIAAAEEAETRGLGVVSLNGKMIDGPIIDHARKVVALSASGIRD

Sequences:

>Translated_289_residues
MKPRRSMLFIPGANAAMLSTSFVYGADAVMFDLEDAVSLREKDTARLLVYQALQHPLYQDIETVVRINPLNTPFGLADLE
AVVRAGVDMVRLPKTDSKEDIHELEAHVERIERECGREVGSTKLMAAIESALGVVNAVEIARASPRLAAIALAAFDYVMD
MGTSRGDGTELFYARCAVLHAARVAGIAAYDVVWSDINNEEGFLAEANLAKNLGFNGKSLVNPRQIELLHQVYAPTRKEV
DHALEVIAAAEEAETRGLGVVSLNGKMIDGPIIDHARKVVALSASGIRD
>Mature_289_residues
MKPRRSMLFIPGANAAMLSTSFVYGADAVMFDLEDAVSLREKDTARLLVYQALQHPLYQDIETVVRINPLNTPFGLADLE
AVVRAGVDMVRLPKTDSKEDIHELEAHVERIERECGREVGSTKLMAAIESALGVVNAVEIARASPRLAAIALAAFDYVMD
MGTSRGDGTELFYARCAVLHAARVAGIAAYDVVWSDINNEEGFLAEANLAKNLGFNGKSLVNPRQIELLHQVYAPTRKEV
DHALEVIAAAEEAETRGLGVVSLNGKMIDGPIIDHARKVVALSASGIRD

Specific function: Represents a citryl-ACP lyase

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit subfamily

Homologues:

Organism=Homo sapiens, GI45545437, Length=299, Percent_Identity=27.4247491638796, Blast_Score=128, Evalue=6e-30,
Organism=Escherichia coli, GI87081764, Length=290, Percent_Identity=64.8275862068965, Blast_Score=370, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17557458, Length=299, Percent_Identity=28.0936454849498, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CITE_KLEPN (P17725)

Other databases:

- EMBL:   X79817
- PIR:   S60775
- ProteinModelPortal:   P17725
- SMR:   P17725
- BRENDA:   4.1.3.34
- BRENDA:   4.1.3.6
- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR006475
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PIRSF:   PIRSF015582
- TIGRFAMs:   TIGR01588

Pfam domain/function: PF03328 HpcH_HpaI; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =4.1.3.6; =4.1.3.34

Molecular weight: Translated: 31352; Mature: 31352

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: BINDING 66-66 BINDING 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPRRSMLFIPGANAAMLSTSFVYGADAVMFDLEDAVSLREKDTARLLVYQALQHPLYQD
CCCCCCEEEECCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCHHHHH
IETVVRINPLNTPFGLADLEAVVRAGVDMVRLPKTDSKEDIHELEAHVERIERECGREVG
HHHHEEECCCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHC
STKLMAAIESALGVVNAVEIARASPRLAAIALAAFDYVMDMGTSRGDGTELFYARCAVLH
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
AARVAGIAAYDVVWSDINNEEGFLAEANLAKNLGFNGKSLVNPRQIELLHQVYAPTRKEV
HHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHH
DHALEVIAAAEEAETRGLGVVSLNGKMIDGPIIDHARKVVALSASGIRD
HHHHHHHHHHHHHHHCCCEEEEECCEEECCHHHHHHHHHEEEECCCCCC
>Mature Secondary Structure
MKPRRSMLFIPGANAAMLSTSFVYGADAVMFDLEDAVSLREKDTARLLVYQALQHPLYQD
CCCCCCEEEECCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCHHHHH
IETVVRINPLNTPFGLADLEAVVRAGVDMVRLPKTDSKEDIHELEAHVERIERECGREVG
HHHHEEECCCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHC
STKLMAAIESALGVVNAVEIARASPRLAAIALAAFDYVMDMGTSRGDGTELFYARCAVLH
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
AARVAGIAAYDVVWSDINNEEGFLAEANLAKNLGFNGKSLVNPRQIELLHQVYAPTRKEV
HHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHH
DHALEVIAAAEEAETRGLGVVSLNGKMIDGPIIDHARKVVALSASGIRD
HHHHHHHHHHHHHHHCCCEEEEECCEEECCHHHHHHHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2205500; 7830578