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The map label for this gene is rutB [H]

Identifier: 151222756

GI number: 151222756

Start: 2803988

End: 2804548

Strand: Reverse

Name: rutB [H]

Synonym: NWMN_2544

Alternate gene names: 151222756

Gene position: 2804548-2803988 (Counterclockwise)

Preceding gene: 151222757

Following gene: 151222754

Centisome position: 97.42

GC content: 34.76

Gene sequence:

>561_bases
ATGTCTCGAAAAACGGCGCTATTAGTTTTGGATATGCAAGAAGGTATAGCGAGTAGTGTACCTAGAATAAAAAATATTAT
TAAAGCGAATCAGAGAGCAATTGAAGCAGCAAGACAACATCGAATACCAGTCATTTTCATACGTTTAGTGTTAGATAAGC
ATTTTAATGATGTCTCCTCGAGTAATAAAGTGTTTTCAACAATTAAAGCTCAAGGATATGCGATTACTGAAGCAGATGCA
TCTACACGAATACTTGAAGATTTAGCACCACTAGAAGATGAGCCGATTATTTCTAAGCGACGCTTTAGCGCATTTACAGG
TAGTTACTTGGAAGTTTATTTACGTGCAAATGATATTAATCATTTAGTATTAACGGGTGTCTCTACAAGTGGAGCTGTAT
TGAGCACGGCATTAGAAAGTGTAGATAAAGACTATTATATTACTGTTTTAGAAGATGCTGTTGGTGATAGATCAGATGAT
AAACATGACTTTATTATTGAACAAATTTTATCACGCTCATGTGACATTGAATCCGTAGAGTCATGGAAAAGTAGTTTATA
G

Upstream 100 bases:

>100_bases
ATTGTGAAGTATATGCAAAATGCAATGAATAGATTGTCCATCATTTTAACGTTATAATGAATTTAACGACTTAGAACTAC
ACAAGTAAAGGAGAATGAAG

Downstream 100 bases:

>100_bases
TTAATATAACGTCAATTAAAGCTCGGCAGTAATGTTTGAGAATAAGTACATTTGCTCATATTTATAAAATGTGTGAGATG
GCAATTGAAACGGATATGAT

Product: isochorismatase family protein

Products: 2,3-Dihydro-2,3-dihydroxybenzoate; Pyruvate [C]

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPVIFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADA
STRILEDLAPLEDEPIISKRRFSAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
KHDFIIEQILSRSCDIESVESWKSSL

Sequences:

>Translated_186_residues
MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPVIFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADA
STRILEDLAPLEDEPIISKRRFSAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
KHDFIIEQILSRSCDIESVESWKSSL
>Mature_185_residues
SRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPVIFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADAS
TRILEDLAPLEDEPIISKRRFSAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDDK
HDFIIEQILSRSCDIESVESWKSSL

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786811, Length=177, Percent_Identity=27.683615819209, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI87081820, Length=202, Percent_Identity=25.2475247524752, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: 3.3.2.1 [C]

Molecular weight: Translated: 20730; Mature: 20599

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPVIFIRLVLDKHFNDVSS
CCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
SNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRFSAFTGSYLEVYLRANDIN
CCHHHHHHHHCCEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEEEECCCC
HLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDDKHDFIIEQILSRSCDIESVE
EEEEECCCCCCHHHHHHHHHCCCCEEEEEEEHHCCCCCCCHHHHHHHHHHHCCCCHHHHH
SWKSSL
HHHHCC
>Mature Secondary Structure 
SRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPVIFIRLVLDKHFNDVSS
CCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
SNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRFSAFTGSYLEVYLRANDIN
CCHHHHHHHHCCEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEEEECCCC
HLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDDKHDFIIEQILSRSCDIESVE
EEEEECCCCCCHHHHHHHHHCCCCEEEEEEEHHCCCCCCCHHHHHHHHHHHCCCCHHHHH
SWKSSL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; Isochorismate [C]

Specific reaction: H2O + Isochorismate --> 2,3-Dihydro-2,3-dihydroxybenzoate + Pyruvate [C]

General reaction: Ether hydrolysis [C]

Inhibitor: Fe2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA