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The map label for this gene is bioB

Identifier: 151222536

GI number: 151222536

Start: 2552810

End: 2553817

Strand: Reverse

Name: bioB

Synonym: NWMN_2324

Alternate gene names: 151222536

Gene position: 2553817-2552810 (Counterclockwise)

Preceding gene: 151222537

Following gene: 151222535

Centisome position: 88.71

GC content: 33.23

Gene sequence:

>1008_bases
ATGAATTTGGCTAAACGCATATTACAAGGGGAACAATTAACAAAAGAGACTGTATTGAAAATTTATGAGGATACTAATAT
TGATACCTTAGATTTATTAAATGAGGCGTACATTTTAAGAAAACATTATTTTGGTAAAAAAGTAAAATTAAACATGATTT
TAAATGCTAAAAGTGGTATATGTCCTGAAAATTGTGGGTACTGTGGACAATCACGAGATATTAAACAAAAACAGCGATAT
GCTTTAATTCCAGAGGAACAAATTATCGATGGAGCAAAGGTGGCACATGATAATCATATTGGAACATATTGTATTGTTAT
GAGTGGTAGAGGACCGAGCGATAAAGAAGTTGATCATATTAGTAATACTGTAAGAACGATTAAATCTCAACACCCGCAAC
TAAAAATCTGTGCATGTTTAGGATTAACGAATGACGAACAAGCTAAGAAACTTAAGTCAGCTGGTGTAGACAGATATAAC
CACAATATTAATACAAGTGAAAATTACCATGATAACGTCGTGACAACGCATAGTTATAAAGATAGAACAGATACGATAGA
ACTAATGAAAGCGAATAATATATCACCATGTTCTGGCGTGATTTGTGGTATGGGAGAATCTAATCAAGATATTGTTGATA
TGGCATTTGCTTTAAAAGAAATGGATGCCGACAGTATTCCGATTAATTTTTTGCATCCAATCAAAGGCACAAAGTTTGGA
AGCATGGATGATTTAACACCAATGAAATGTTTAAGAATCGTAGCATTATTCCGATTAATCAATCCTACGAAAGAAATTCG
TATTGCTGGAGGAAGAGAGGTCAACTTACGTTCGTTACAGCCATTAGCATTAAAAGCGGCGAATTCAATATTTGTCGGTG
ATTATCTAATTACTGGTGGGCAACCGAACCAATTAGACTACGATATGATTAATGATTTAGGCTTTGAAATTGATTATGAC
ACTTGTGAAAATAAGGAAAACAAGAATGAAGTTTCAAGAGCAAATTGA

Upstream 100 bases:

>100_bases
ACGTTTGTGCCAGTGTTAAGTATGTCAAATAAAGAAGTGAAAACGATGGTACGTATTTTTAAAAAGGCAGTACATAACAT
TTTAGATAGGAAGTGTTAAT

Downstream 100 bases:

>100_bases
TTTATTACAACAGAAAGGGTTATATCGGTCGCTTAAATCGGTAGGGTATATAGATAGACAGTATATTGAAGTAGAAAATA
AGAGATGTACGAACTATACA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYFGKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRY
ALIPEEQIIDGAKVAHDNHIGTYCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFALKEMDADSIPINFLHPIKGTKFG
SMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNLRSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYD
TCENKENKNEVSRAN

Sequences:

>Translated_335_residues
MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYFGKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRY
ALIPEEQIIDGAKVAHDNHIGTYCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFALKEMDADSIPINFLHPIKGTKFG
SMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNLRSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYD
TCENKENKNEVSRAN
>Mature_335_residues
MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYFGKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRY
ALIPEEQIIDGAKVAHDNHIGTYCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFALKEMDADSIPINFLHPIKGTKFG
SMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNLRSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYD
TCENKENKNEVSRAN

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=307, Percent_Identity=32.2475570032573, Blast_Score=177, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6321725, Length=304, Percent_Identity=32.8947368421053, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 37559; Mature: 37559

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYFGKKVKLNMILNAKSGI
CCHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCC
CPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGTYCIVMSGRGPSDKEVDHI
CCCCCCCCCCCCCHHHHHHEECCCHHHHCCCHHHCCCCCCCEEEEEEECCCCCCCHHHHH
SNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYNHNINTSENYHDNVVTTHSYK
HHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCCHHCCCCCCCCCCCCCEEEECCCC
DRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFALKEMDADSIPINFLHPIKGTKFG
CCCCEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
SMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNLRSLQPLALKAANSIFVGDYLITGG
CCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCEECCCCCCHHHHHHCCCEEEEEEEEECC
QPNQLDYDMINDLGFEIDYDTCENKENKNEVSRAN
CCCCCCHHHHHHCCCEEECHHHCCCCCCCHHCCCC
>Mature Secondary Structure
MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYFGKKVKLNMILNAKSGI
CCHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCC
CPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGTYCIVMSGRGPSDKEVDHI
CCCCCCCCCCCCCHHHHHHEECCCHHHHCCCHHHCCCCCCCEEEEEEECCCCCCCHHHHH
SNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYNHNINTSENYHDNVVTTHSYK
HHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCCCCHHCCCCCCCCCCCCCEEEECCCC
DRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFALKEMDADSIPINFLHPIKGTKFG
CCCCEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
SMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNLRSLQPLALKAANSIFVGDYLITGG
CCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCEECCCCCCHHHHHHCCCEEEEEEEEECC
QPNQLDYDMINDLGFEIDYDTCENKENKNEVSRAN
CCCCCCHHHHHHCCCEEECHHHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA